scholarly journals Bone formation potential of collagen type I-based recombinant peptide particles in rat calvaria defects

2021 ◽  
Vol 16 ◽  
pp. 12-22
Author(s):  
Yasunori Akiyama ◽  
Masaaki Ito ◽  
Taku Toriumi ◽  
Takahiro Hiratsuka ◽  
Yoshinori Arai ◽  
...  
Keyword(s):  
Bone Formation ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Recombinant Peptide ◽  
Formation Potential ◽  
Rat Calvaria

scholarly journals Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo

2012 ◽  
Vol 23 (9) ◽  
pp. 2227-2233 ◽  
Author(s):  
Markus D. Schofer ◽  
Lisa Tünnermann ◽  
Hendric Kaiser ◽  
Philip P. Roessler ◽  
Christina Theisen ◽  
...  
Keyword(s):  
Bone Formation ◽  
Collagen Type ◽  
Collagen Type I ◽  
Cooperative Effect ◽  
Type I ◽  
Nanofiber Scaffolds

scholarly journals Serum N-Terminal Propeptide of Collagen Type I is Associated with the Number of Bone Metastases in Breast and Prostate Cancer and Correlates to Other Bone Related Markers

2011 ◽  
Vol 3 ◽  
pp. BIC.S6484 ◽  
Author(s):  
D.J. Leeming ◽  
M. Koizumi ◽  
P. Qvist ◽  
V. Barkholt ◽  
C. Zhang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Resorption ◽  
Bone Formation ◽  
Bone Metastases ◽  
Cancer Patients ◽  
Collagen Type ◽  
Collagen Type I ◽  
Tartrate Resistant Acid Phosphatase ◽  
Type I ◽  
Breast And Prostate Cancer

Background A number of biomarkers have been proven potentially useful for their ability to indicate bone metastases (BM) in cancer patients. The aim of this study was to investigate the relative utility of a newly developed N-terminal propeptide of collagen type I (PINP) human serum assay for the detection of BM in cancer patients. This assay has a corresponding rat PINP assay which in the future might help in translational science between rodent and human trials. Methods Participants were 161 prostate, lung and breast cancer patients stratified by number of BM(Soloway score). PINP was assessed and correlated to number of BM. Additionally, the PINP marker was correlated to bone resorption of young (ALPHA CTX-I)- and aged bone (BETA CTX-I); number of osteoclasts (Tartrate-resistant acid phosphatase 5b, TRACP5B) and osteoclast activity (CTX-I/TRACP5B). Results PINP was significantly elevated in breast- and prostate cancer patients +BM, compared to –BM ( P < 0.001), however not in lung cancer patients. A strong linear association was seen between PINP and the number of BMs. Significant elevation of PINP was observed at Soloway scores 1–4 (<0 BM) compared with score 0 (0 BM) ( P < 0.001). The correlation between bone resorption of young bone or aged bone and bone formation was highly significant in patients +BM and –BM ( P < 0.0001). Conclusions Data suggest that the present PINP potentially could determine skeletal involvement in patients with breast or prostate cancer. Correlations suggested that coupling between bone resorption and bone formation was maintained in breast- and prostate cancer patients.

scholarly journals Osteogenesis and mineralization of mesenchymal stem cells in collagen type I-based recombinant peptide scaffolds

2017 ◽  
Vol 105 (7) ◽  
pp. 1856-1866 ◽  
Author(s):  
Kendell M. Pawelec ◽  
Davide Confalonieri ◽  
Franziska Ehlicke ◽  
Huibert A. van Boxtel ◽  
Heike Walles ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Recombinant Peptide ◽  
Peptide Scaffolds

scholarly journals Relative rates of biosynthesis of collagen type I, type V and type VI in calf cornea

1991 ◽  
Vol 274 (2) ◽  
pp. 615-617 ◽  
Author(s):  
P Kern ◽  
M Menasche ◽  
L Robert
Keyword(s):  
Type I Collagen ◽  
Collagen Type ◽  
Corneal Stroma ◽  
Collagen Type I ◽  
Type I ◽  
Type Vi Collagen ◽  
Sds Page ◽  
Proline Incorporation ◽  
Type V

The biosynthesis of type I, type V and type VI collagens was studied by incubation of calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated by salt fractionation and quantified by SDS/PAGE. Expressed as proportions of the total hydroxyproline solubilized, corneal stroma comprised 75% type I, 8% type V and 17% type VI collagen. The rates of [3H]proline incorporation, linear up to 24 h for each collagen type, were highest for type VI collagen and lowest for type I collagen. From pulse-chase experiments, the calculated apparent half-lives for types I, V and VI collagens were 36 h, 10 h and 6 h respectively.

scholarly journals Confocal Blood Flow Videomicroscopy of Thrombus Formation over Human Arteries and Local Targeting of P2X7

2021 ◽  
Vol 22 (8) ◽  
pp. 4066
Author(s):  
Patrizia Marchese ◽  
Maria Lombardi ◽  
Maria Elena Mantione ◽  
Domenico Baccellieri ◽  
David Ferrara ◽  
...  
Keyword(s):  
Blood Flow ◽  
Vascular Function ◽  
Collagen Type ◽  
Thrombus Formation ◽  
Collagen Type I ◽  
Type I ◽  
Prevention Therapy ◽  
Healthy Donors ◽  
Internal Mammary ◽  
Human Arteries

Atherothrombosis exposes vascular components to blood. Currently, new antithrombotic therapies are emerging. Herein we investigated thrombogenesis of human arteries with/without atherosclerosis, and the interaction of coagulation and vascular components, we and explored the anti-thrombogenic efficacy of blockade of the P2X purinoceptor 7 (P2X7). A confocal blood flow videomicroscopy system was performed on cryosections of internal mammary artery (IMA) or carotid plaque (CPL) determining/localizing platelets and fibrin. Blood from healthy donors elicited thrombi over arterial layers. Confocal microscopy associated thrombus with tissue presence of collagen type I, laminin, fibrin(ogen) and tissue factor (TF). The addition of antibodies blocking TF (aTF) or factor XI (aFXI) to blood significantly reduced fibrin deposition, variable platelet aggregation and aTF + aFXI almost abolished thrombus formation, showing synergy between coagulation pathways. A scarce effect of aTF over sub-endothelial regions, more abundant in tissue TF and bundles of laminin and collagen type I than deep intima, may suggest tissue thrombogenicity as molecular structure-related. Consistently with TF-related vascular function and expression of P2X7, the sections from CPL but not IMA tissue cultures pre-treated with the P2X7 antagonist A740003 demonstrated poor thrombogenesis in flow experiments. These data hint to local targeting studies on P2X7 modulation for atherothrombosis prevention/therapy.

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