Evaluation of antidiabetic and wound healing properties of ethanol extract of Hedera nepalensis in alloxan-induced diabetic rats

Impaired wound healing in diabetic patients is a serious complication that often leads to amputation or even death with limited effective treatments. Tuo-Li-Xiao-Du-San (TLXDS), a traditional Chinese medicine formula for refractory wounds, has been prescribed for nearly 400 years in China and shows good efficacy in promoting healing. In this study, we explored the effect of TLXDS on healing of diabetic wounds and investigated underlying mechanisms. Four weeks after intravenous injection of streptozotocin, two full-thickness excisional wounds were created with a 10 mm diameter sterile biopsy punch on the back of rats. The ethanol extract of TLXDS was given once daily by oral gavage. Wound area, histological change, inflammation, angiogenesis, and collagen synthesis were evaluated. TLXDS treatment significantly accelerated healing of diabetic rats and improved the healing quality. These effects were associated with reduced neutrophil infiltration and macrophage accumulation, enhanced angiogenesis, and increased collagen deposition. This study shows that TLXDS improves diabetes-impaired wound healing.

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Study on the Effect of Cerium Oxide Nanocubes on Full-Thickness Cutaneous Wound Healing of Type 2 Diabetic Rats

Diabetes ◽

10.2337/db18-643-p ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 643-P ◽

Cited By ~ 2

Author(s):

YANFEI HAN ◽

LINDONG LI ◽

YANJUN LIU ◽

YOU WANG ◽

CHUNHUA YAN ◽

...

Keyword(s):

Wound Healing ◽

Cerium Oxide ◽

Diabetic Rats ◽

Cutaneous Wound Healing ◽

Full Thickness ◽

Cutaneous Wound ◽

Type 2 Diabetic

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Effect of Hydroalcoholic Extract of Teucrium polium on the Process of Wound Healing in Diabetic Rats

passer ◽

10.24271/psr.09 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Mohsin Salih

Keyword(s):

Wound Healing ◽

Diabetic Rats ◽

Hydroalcoholic Extract ◽

Teucrium Polium

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Effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing

Journal of Chinese Integrative Medicine ◽

10.3736/jcim20070612 ◽

2007 ◽

Vol 5 (6) ◽

pp. 661-664 ◽

Cited By ~ 2

Author(s):

Fulun Li

Keyword(s):

Wound Healing ◽

Granulation Tissue ◽

Diabetic Rats

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Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530321666201230113018 ◽

2020 ◽

Vol 21 ◽

Author(s):

Hana M. Hammad ◽

Amer Imraish ◽

Maysa Al-Hussaini ◽

Malek Zihlif ◽

Amani A. Harb ◽

...

Keyword(s):

Wound Healing ◽

Rural Communities ◽

Ex Vivo ◽

Ethanol Extract ◽

Aortic Ring ◽

Treated Group ◽

Control Group ◽

Dependent Manner ◽

Achillea Fragrantissima

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

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Acidified Nitrite Accelerates Wound Healing in Type 2 Diabetic Male Rats: A Histological and Stereological Evaluation

Molecules ◽

10.3390/molecules26071872 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1872

Author(s):

Hamideh Afzali ◽

Mohammad Khaksari ◽

Sajad Jeddi ◽

Khosrow Kashfi ◽

Mohammad-Amin Abdollahifar ◽

...

Keyword(s):

Wound Healing ◽

Fibrous Tissue ◽

Diabetic Rats ◽

Male Rats ◽

Numerical Density ◽

Basal Cells ◽

Diabetic Wound Healing ◽

Diabetic Wounds ◽

Endothelial Growth Factor

Impaired skin nitric oxide production contributes to delayed wound healing in type 2 diabetes (T2D). This study aims to determine improved wound healing mechanisms by acidified nitrite (AN) in rats with T2D. Wistar rats were assigned to four subgroups: Untreated control, AN-treated control, untreated diabetes, and AN-treated diabetes. AN was applied daily from day 3 to day 28 after wounding. On days 3, 7, 14, 21, and 28, the wound levels of vascular endothelial growth factor (VEGF) were measured, and histological and stereological evaluations were performed. AN in diabetic rats increased the numerical density of basal cells (1070 ± 15.2 vs. 936.6 ± 37.5/mm3) and epidermal thickness (58.5 ± 3.5 vs. 44.3 ± 3.4 μm) (all p < 0.05); The dermis total volume and numerical density of fibroblasts at days 14, 21, and 28 were also higher (all p < 0.05). The VEGF levels were increased in the treated diabetic wounds at days 7 and 14, as was the total volume of fibrous tissue and hydroxyproline content at days 14 and 21 (all p < 0.05). AN improved diabetic wound healing by accelerating the dermis reconstruction, neovascularization, and collagen deposition.

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