scholarly journals Protoplast isolation and development of a transient expression system for sweet cherry (Prunus avium L.)

2016 ◽  
Vol 209 ◽  
pp. 14-21 ◽  
Author(s):  
Liping Yao ◽  
Xiong Liao ◽  
Zhizhe Gan ◽  
Xiang Peng ◽  
Peng Wang ◽  
...  
2013 ◽  
Vol 150 ◽  
pp. 206-212 ◽  
Author(s):  
Hongyu Huang ◽  
Zhenyu Wang ◽  
Jintao Cheng ◽  
Wenchao Zhao ◽  
Xin Li ◽  
...  

2020 ◽  
Vol 21 (7) ◽  
pp. 2264 ◽  
Author(s):  
Rui Ren ◽  
Jie Gao ◽  
Chuqiao Lu ◽  
Yonglu Wei ◽  
Jianpeng Jin ◽  
...  

Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for Cymbidium orchids. Herein, we established an efficient protoplast isolation protocol from orchid petals through optimization of enzymatic conditions. It requires optimal D-mannitol concentration (0.5 M), enzyme concentration (1.2 % (w/v) cellulose and 0.6 % (w/v) macerozyme) and digestion time (6 h). With this protocol, the highest yield (3.50 × 107/g fresh weight of orchid tissue) and viability (94.21%) of protoplasts were obtained from flower petals of Cymbidium. In addition, we achieved high transfection efficiency (80%) through the optimization of factors affecting polyethylene glycol (PEG)-mediated protoplast transfection including incubation time, final PEG4000 concentration and plasmid DNA amount. This highly efficient protoplast-based transient expression system (PTES) was further used for protein subcellular localization, bimolecular fluorescence complementation (BiFC) assay and gene regulation studies of flowering related genes in Cymbidium orchids. Taken together, our protoplast isolation and transfection protocol is highly efficient, stable and time-saving. It can be used for gene function and molecular analyses in orchids and other economically important monocot crops.


2021 ◽  
Vol 175 ◽  
pp. 111494
Author(s):  
Excequel Ponce ◽  
Blanca Alzola ◽  
Natalia Cáceres ◽  
Madeline Gas ◽  
Catalina Ferreira ◽  
...  

2008 ◽  
Vol 4 (4) ◽  
pp. 897-910 ◽  
Author(s):  
James W. Olmstead ◽  
Audrey M. Sebolt ◽  
Antonio Cabrera ◽  
Suneth S. Sooriyapathirana ◽  
Sue Hammar ◽  
...  

2017 ◽  
Vol 8 ◽  
Author(s):  
Xiliang Qi ◽  
Congli Liu ◽  
Lulu Song ◽  
Yuhong Li ◽  
Ming Li

Author(s):  
Michaela Benková ◽  
Iveta Čičová ◽  
Daniela Benedikova ◽  
Lubomir Mendel ◽  
Miroslav Glasa

Abstract The work is focused on the evaluation of variability of morphological and pomological characteristics of several old sweet cherries (Prunus avium L.) that were found in different Slovak regions. The experimental work has been performed during two years, 2014 and 2015. The following characteristics according to the descriptor list of subgenus Cerasus were evaluated - period of flowering and ripening, morphological characteristics of the flowers, fruit size, fruit weight, and quality parameters. The results showed high variability of evaluated accessions. From the 13 surveyed localities, the most valuable accessions were found in the locality Hornį Streda - places Čachtice, Krakovany, Nitra, and Brdárka. During the collecting expeditions, 170 accessions of sweet cherry, with fruit of the different quality were found. The most interesting accessions were grafted onto rootstocks with different intensity of growth (Prunus avium L., Prunus mahaleb L., and ‘Gisela5’). Some of the selected cherry accessions can be used for commercial growing after tests, while some of them can be used only for collection of genetic resources and as potential genitors in breeding programmes.


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