In vitro efficiency of embryo rescue of intra- and interspecific hybrid crosses of sweet cherry and Chinese cherry cultivars

Abstract Arachis hypogaea L. cv. NC 4 was used as a model plant system in an effort to develop an in vitro embryo rescue protocol which could have application to interspecific hybrid embryos, which often abort at very early growth stages. Embryo growth and development was studied in 1- to 4-day-old peg tips containing proembryos equivalent to a stage where many interspecific hybrid embryos abort. Three independent experiments were conducted to 1) determine the most favorable basal media, 2) evaluate the effects of auxins and cytokinins on growth, and 3) determine a favorable combination of auxins and cytokinins for in vitro peanut embryo growth. The results indicated that MS (Murashige and Skoog) medium with 3% sucrose was the most favorable basal medium among seven media and two sucrose concentrations analyzed. IAA (indole-3-acetic acid) at 1.5 mg L-1 in combination with a range of KN (kinetin) levels from 0.5 to 1.25 mg L-1 were the growth regulator combinations of choice. Proembryo growth reached the multicellular globular stage, but differentiation into heart-shaped embryos did not occur.

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In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2015.14577 ◽

2015 ◽

Vol 14 (27) ◽

pp. 2191-2201

Author(s):

Buttibwa Mary ◽

S Kawuki Robert ◽

K Tugume Arthur ◽

Akol Jacinta ◽

Magambo Stephen ◽

...

Keyword(s):

Plant Regeneration ◽

Manihot Esculenta ◽

Embryo Rescue ◽

Manihot Esculenta Crantz ◽

Irradiated Pollen ◽

Self Pollination

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Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202010000300008 ◽

2010 ◽

Vol 22 (3) ◽

pp. 209-215 ◽

Cited By ~ 12

Author(s):

Zanderluce G. Luis ◽

Kadja Milena G. Bezerra ◽

Jonny Everson Scherwinski-Pereira

Keyword(s):

Oil Palm ◽

Embryo Rescue ◽

Morphological Characteristics ◽

Growth Conditions ◽

Epidermal Cells ◽

In Vitro Cultivation ◽

Vascular Bundles ◽

Anatomical Plasticity ◽

Germinated Seeds

Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal and hypodermal thickness, and the values for the expansion cells and leaf mesophile. Anatomically, the oil palm leaves present the same tissues composition in both growth conditions, with uniseriate epidermal cells, and tetracitic stomata occurring in both epidermal surfaces. Epidermal cells from in vitro plants are thinner than ones from greenhouse. The midrib of leaves from greenhouse plants are more developed and is composed by only one central vascular bundle, while plants from in vitro cultivation developed three to four collateral vascular bundles.

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Interspecific Hybridization Of Red Clover (Trifolium Pratense L.) With Alsike Clover (Trifolium Hybndum L.) Using In Vitro Embryo Rescue

Genetic Manipulation in Plant Breeding ◽

10.1515/9783110871944-081 ◽

1986 ◽

pp. 469-472

Keyword(s):

Interspecific Hybridization ◽

Trifolium Pratense ◽

Embryo Rescue ◽

Red Clover ◽

Trifolium Pratense L

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Effect of Plant Growth Regulators on in vitro Propagation of Vitis ficifolia var. ganebu and its Interspecific Hybrid Grape

Asian Journal of Plant Sciences ◽

10.3923/ajps.2005.466.471 ◽

2005 ◽

Vol 4 (5) ◽

pp. 466-471 ◽

Cited By ~ 1

Author(s):

Puspa Raj Poudel ◽

Ikuo Kataoka . ◽

Ryosuke Mochioka .

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Interspecific Hybrid ◽

In Vitro Propagation

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Enhanced Seed Germination of Ilex dumosa R. (Aquifoliaceae) through In Vitro Culture of Cut Pyrenes

HortScience ◽

10.21273/hortsci.46.2.278 ◽

2011 ◽

Vol 46 (2) ◽

pp. 278-281 ◽

Cited By ~ 3

Author(s):

Natalia R. Dolce ◽

Luis A. Mroginski ◽

Hebe Y. Rey

Keyword(s):

In Vitro Culture ◽

Embryo Rescue ◽

Germination Percentage ◽

Effective Technique ◽

Cutting Surface ◽

Rescue Technique ◽

Growth Room ◽

Scalpel Blade ◽

Culture Protocol

An in vitro culture protocol was developed that increased the germination percentage and decreased the lag time to germination for Ilex dumosa R. pyrenes as a tool for replacing the laborious task of embryo rescue technique. This method involves transversely cutting surface-sterilized pyrenes with a scalpel blade, then placing the micropylar one-third end with the rudimentary embryo (≈0.25 mm long) on solidified (agar 0.65%) quarter-strength salts and vitamins of Murashige and Skoog, 1962 medium with 3% sucrose, and incubating in a growth room at 27 ± 2 °C with a 14-h photoperiod (116 μmol·m−2·s−1). Most of the cut pyrenes (greater than 50%) germinated within the first month after inoculation and achieved maximum germination (≈70%) in 2 months compared with whole pyrenes, which began to germinate 3 months after sowing and required more than 8 months for maximum germination (37%). Moreover, the germination percentage of cut pyrenes was significantly higher than the germination of isolated embryos (34%). Thus, the cut pyrenes culture is a simpler and more effective technique than embryo rescue. Easily, on average, a trained operator is able to culture ≈1000 cut pyrenes per day instead of ≈100 isolated embryos.

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The Profile and Content of Polyphenols and Carotenoids in Local and Commercial Sweet Cherry Fruits (Prunus avium L.) and Their Antioxidant Activity In Vitro

Antioxidants ◽

10.3390/antiox8110534 ◽

2019 ◽

Vol 8 (11) ◽

pp. 534 ◽

Cited By ~ 9

Author(s):

Dominika Średnicka-Tober ◽

Alicja Ponder ◽

Ewelina Hallmann ◽

Agnieszka Głowacka ◽

Elżbieta Rozpara

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Sweet Cherry ◽

Prunus Avium ◽

Sweet Cherries ◽

High Antioxidant Activity ◽

Positive Correlations ◽

Sweet Cherry Cultivars ◽

Prunus Avium L

The aim of this study was to evaluate and compare the content of a number of bioactive compounds and antioxidant activity of fruits of selected local and commercial sweet cherry (Prunus avium L.) cultivars. The experiment showed that the selected cultivars of sweet cherries differ significantly in the content of polyphenolic compounds and carotenoids. The fruits of commercial sweet cherry cultivars were, on average, richer in polyphenols (the sum of phenolic compounds determined chromatographically), flavonoids, as well as anthocyanins and were characterized by higher antioxidant activity when compared to the local, traditional cultivars. In the group of the traditional sweet cherry cultivars, particular attention could be paid to Black Late cv., showing the highest antioxidant activity of fruits. In the group of commercial sweet cherry cultivars, Cordia and Sylvia fruits could be recognized as being rich in bioactive compounds with high antioxidant activity. Yellow skin cultivars were characterized by the highest concentrations of carotenoids. Strong positive correlations between the identified bioactive compounds and antioxidant activity of fruits were also found. Although different cultivars of sweet cherries show a high variability in phenolics and carotenoids profiles as well as in the antioxidant activity of fruits, they all should be, similarly to other types of cherries, recognized as a rich source of bioactive compounds with an antioxidant potential.

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Embryo rescue from interspecific crosses in apple rootstocks

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2006000600011 ◽

2006 ◽

Vol 41 (6) ◽

pp. 969-973 ◽

Cited By ~ 5

Author(s):

Adriana Cibele de Mesquita Dantas ◽

José Itamar Boneti ◽

Rubens Onofre Nodari ◽

Miguel Pedro Guerra

Keyword(s):

Culture Medium ◽

Culture Media ◽

Embryo Rescue ◽

Casein Hydrolysate ◽

Female Parent ◽

Interspecific Crosses ◽

Open Pollination ◽

Apple Rootstocks ◽

Hand Pollination

The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from hand-pollination, cultivated in medium supplemented with 14 µM IAA, 5 µM kinetin and 1.5 µM Ga3 (MS1), mainly those of M. prunifolia x M. pumila, showed a high percentage of rusted embryos (96.2%). Embryos from open pollination of M. prunifolia and M. pumila formed calluses. It was possible to identify the influence of the female parent by the enhanced development of M. pumila shoots derived from open or hand-pollination. The crossing of responsive species and the use of the technique of embryo culture provided a rapid and uniform germination and, consequently, the development of fully normal seedlings.

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