Approach to New peach rootstocks resistant to root-knot nematodes (Meloidogyne species) selected from local Mit-Ghamer peach cultivar

Abstract The objectives of this study were to determine the efficacy, range of activity and effective concentrations (EC50 and EC80) of abamectin as a seed treatment of tomato against Meloidogyne incognita, M. arenaria and M. javanica. The study revealed that abamectin seed treatment at concentrations ranging between 0.3 and 1 mg a.s. seed−1 is highly effective against the three species of root-knot nematodes, retaining its efficacy in the soil for 8 weeks. The highest EC50 found was reached at 0.2 mg a.s. seed−1. The highest EC80 for the number of egg masses per g root in the three Meloidogyne species was attained at 0.51 mg a.s. seed−1. Abamectin as a seed treatment is promising for the control of root-knot nematodes in the field since only low amounts of active ingredient are required to give adequate protection in the most sensitive stages of tomato root growth and development.

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Differences in Feeding Sites Induced by Root-Knot Nematodes, Meloidogyne spp., in Chickpea

Phytopathology ◽

10.1094/phyto-95-0368 ◽

2005 ◽

Vol 95 (4) ◽

pp. 368-375 ◽

Cited By ~ 19

Author(s):

Nicola Vovlas ◽

Hava F. Rapoport ◽

Rafael M. Jiménez Díaz ◽

Pablo Castillo

Keyword(s):

Giant Cell ◽

Faba Bean ◽

Nematode Species ◽

Giant Cells ◽

The Other ◽

Root Knot Nematodes ◽

Feeding Site ◽

Feeding Sites ◽

Meloidogyne Spp ◽

Meloidogyne Species

Root-knot nematodes (Meloidogyne spp.) are sedentary, obligate endoparasites in plants, where they induce specialized feeding sites. The feeding sites act as strong metabolic sinks to which photosynthates are mobilized. The histopathological modifications in the nematode-induced feeding sites of artificially inoculated chickpea cv. UC 27 were qualitatively and quantitatively compared using five isolates of M. artiellia and one isolate each of M. arenaria, M. incognita, and M. javanica. All Meloidogyne isolates infected chickpea plants, but root gall thickening was significantly less for M. artiellia isolates than for the other Meloidogyne species. Nevertheless, neither the number of giant cells in the feeding site (averaging four to six) nor the area of individual giant cells was influenced by nematode species or isolate. However, the number of nuclei per giant cell was significantly smaller, and the maximum diameters of nuclei and nucleoli were significantly greater, in giant cells induced by M. artiellia isolates than in those induced by M. arenaria, M. incognita, or M. javanica. In a second experiment, M. artiellia-induced giant cells in faba bean and rapeseed also contained a small number of large nuclei.

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Meloidogyne Species Associated with Weeds in Rio Grande do Sul

Planta Daninha ◽

10.1590/s0100-83582019370100095 ◽

2019 ◽

Vol 37 ◽

Cited By ~ 1

Author(s):

C. BELLÉ ◽

T.E. KASPARY ◽

R.R. BALARDIN ◽

R.F. RAMOS ◽

Z.I. ANTONIOLLI

Keyword(s):

Host Plants ◽

Management Practices ◽

Rio Grande ◽

Meloidogyne Javanica ◽

Rio Grande Do Sul ◽

Weed Species ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Meloidogyne Spp ◽

Meloidogyne Species

ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was detected in 15.6% of the samples. M. incognita Est. I2 (Rm: 1.0, 1.1), M. ethiopica Est. E3 (Rm: 0.9, 1.15, 1.30), M. enterolobii Est. M2 (Rm: 0.7, 0.75, 0.9, 0.95) and M. hapla Est. H1 (Rm: 1.17) in 13.3%, 8.9%, 6.7% and 2.2% of the samples, respectively. Therefore, knowledge of the range of host plants to different species of the root-knot nematode can positively contribute to the adoption of management practices that allow the reduction of their populations in the soil.

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Penetration and Reproduction of Four Major Root-knot Nematodes, Meloidogyne species, in Sweet Potato, Ipomea batatas

Nematological Research (Japanese Journal of Nematology) ◽

10.3725/jjn1993.31.1-2_37 ◽

2001 ◽

Vol 31 (1-2) ◽

pp. 37-42 ◽

Cited By ~ 3

Author(s):

Zen-ichi SANO ◽

Hideaki IWAHORI

Keyword(s):

Sweet Potato ◽

Root Knot Nematodes ◽

Ipomea Batatas ◽

Meloidogyne Species

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Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks

Genome ◽

10.1139/g98-008 ◽

1998 ◽

Vol 41 (2) ◽

pp. 199-207 ◽

Cited By ~ 55

Author(s):

Zhen-Xiang Lu ◽

B Sosinski ◽

G L Reighard ◽

W V Baird ◽

A G Abbott

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Prunus Persica ◽

Aflp Marker ◽

Aflp Markers ◽

Single Linkage ◽

Root Knot Nematodes ◽

Group I ◽

Peach Rootstocks

A genetic linkage map for peach (Prunus persica (L.) Batsch) rootstocks has been constructed using amplified fragment length polymorphism (AFLP) markers in 55 F2 individuals derived from the cross Lovell x Nemared. From 21 different primer combinations, a total of 169 AFLP markers were scored, of which, 153 were assigned to 15 linkage groups covering 1297 centimorgans (cM) of the peach nuclear genome. The average interval between these markers was 9.1 cM. Two genes (Mi and Mij) involved in resistance to root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood and Meloidogyne javanica (Treub) Chitwood) were mapped to a single linkage group (Group I). These two loci were separated by a 16.5-cM interval. One codominant AFLP marker (EAA/MCAT10) was tightly linked to the Mij locus (3.4 cM), and a dominant AFLP marker (EAT/MCAT2) was found to be closely associated with the Mi locus (6.0 cM). These markers are being studied for utilization in peach rootstock breeding with marker-assisted selection.Key words: peach rootstocks, root-knot nematodes, resistance, AFLP, mapping.

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Root-knot nematodes (Meloidogyne spp.) in Europe

Nematology ◽

10.1163/138855410x526831 ◽

2011 ◽

Vol 13 (1) ◽

pp. 3-16 ◽

Cited By ~ 115

Author(s):

Wim Wesemael ◽

Nicole Viaene ◽

Maurice Moens

Keyword(s):

Management Strategies ◽

Resistance Breeding ◽

Common Species ◽

Molecular Techniques ◽

Morphological Identification ◽

Meloidogyne Hapla ◽

Root Knot Nematodes ◽

Important Species ◽

Meloidogyne Spp ◽

Meloidogyne Species

AbstractIn Europe, root-knot nematodes are increasingly important. Out of more than 90 Meloidogyne species currently described, 23 have been found on the continent. In the cooler climates, Meloidogyne hapla, M. naasi, M. chitwoodi and M. fallax are prevalent. Meloidogyne arenaria, M. javanica and M. incognita are the most common species in warmer conditions of southern Europe, but also in glasshouses in northern Europe. Morphological identification of root-knot nematodes is difficult and time consuming; therefore, many research groups have been developing molecular techniques for identification of Meloidogyne species. Meloidogyne chitwoodi and M. fallax are quarantine organisms and subject to regulations, and the highly aggressive M. enterolobii has been added to the EPPO alert list. Differences between temperate and tropical Meloidogyne species and their prevalence in Europe imply the need for different management strategies in south and north Europe. Possible crop rotations for the control of root-knot nematodes are limited due to the wide host range of several important species. The banning of methyl bromide and restrictions on other fumigant pesticides in the EU have increased the application of biofumigation significantly in south Europe. The egg-parasitising fungus Paecilomyces lilacinus is commercialised in Germany and applied as dispersible granules for application in water. Intensive research is conducted on the egg-parasitising fungus Pochonia chlamydosporia, and the obligate parasitic bacterium Pasteuria penetrans. European research has paid much attention to resistance breeding and selection. The Mi gene of tomato is widely used but resistance-breaking populations of M. incognita and M. javanica have been reported in different countries.

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Suitable Hosts of Root Knot Nematode Attack: An Assessment on the Basis of Morphological Size Variations and Population Density under Field Conditions

International Journal of Phytopathology ◽

10.33687/phytopath.004.02.1239 ◽

2015 ◽

Vol 4 (2) ◽

pp. 87-92 ◽

Cited By ~ 1

Author(s):

Gamini Sahu ◽

Surendra K. Gautam ◽

Aditi N. Poddar

Keyword(s):

Population Density ◽

Crop Plants ◽

The Body ◽

Field Conditions ◽

Vegetable Crops ◽

Vegetable Crop ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Body Sizes ◽

Meloidogyne Species

Root knot nematodes (Meloidogyne species) are major pests of vegetable crops causing serious losses in quantity and quality of crop yield. This study involves an assessment of their suitable hosts on the basis of variations in their population densities and body sizes in different vegetable crop plants under field conditions. A random survey of root knot nematode infestation in vegetable crops was conducted in 9 villages of Abhanpur block, Chhattisgarh state, Central India, from January 2012 to March 2012. Diseased plants were identified on the basis of above ground symptoms and soil and root samples collected by digging. Extraction of nematodes was done by Cobb’s sieving and decantation method and Baerman’s funnel technique. Identification was done microscopically by morphological examination of perineal patterns of female nematodes. Body sizes were measured by using an eyepiece/ocular micrometer. Twenty nine percent of the total farm area surveyed suffered from root knot nematode attack. Among the several genera of vegetable crop plants surveyed, Lycopersicon esculentum, Dolichos lablab, Solanum melongena, Momordica charantia, Daucus carota,Capsicum annum,Cucumis sativus had root galls. Three species of root knot nematodes Meloidogyne incognita, M. javanica, M. areneria were identified from the above hosts and a comparative morphometric analysis of the body, head and neck size ratios of females were done. Non-significant body and head size variations existing between the females from D. lablab, S. melongena, C. annum, D. carota, L. esculentum showed that all the crops are equally susceptible to root knot nematode attack. However, on the basis of nematode population density, D. carrota appears to be the best suitable host of the Meloidogyne species other than L. esculentum and S. melongena.

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