scholarly journals Meloidogyne Species Associated with Weeds in Rio Grande do Sul

2019 ◽  
Vol 37 ◽  
Author(s):  
C. BELLÉ ◽  
T.E. KASPARY ◽  
R.R. BALARDIN ◽  
R.F. RAMOS ◽  
Z.I. ANTONIOLLI
Keyword(s):  
Host Plants ◽  
Management Practices ◽  
Rio Grande ◽  
Meloidogyne Javanica ◽  
Rio Grande Do Sul ◽  
Weed Species ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Meloidogyne Spp ◽  
Meloidogyne Species

ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was detected in 15.6% of the samples. M. incognita Est. I2 (Rm: 1.0, 1.1), M. ethiopica Est. E3 (Rm: 0.9, 1.15, 1.30), M. enterolobii Est. M2 (Rm: 0.7, 0.75, 0.9, 0.95) and M. hapla Est. H1 (Rm: 1.17) in 13.3%, 8.9%, 6.7% and 2.2% of the samples, respectively. Therefore, knowledge of the range of host plants to different species of the root-knot nematode can positively contribute to the adoption of management practices that allow the reduction of their populations in the soil.

scholarly journals Marigold (Tagete erecta): An Effective Meloidogyne incognita Trap Plant

2021 ◽  
Vol 25 (02) ◽  
pp. 271-276
Author(s):  
Wentao Wu
Keyword(s):  
Root Exudates ◽  
Host Plants ◽  
Inhibitory Effect ◽  
Root Knot Nematode ◽  
Root Cells ◽  
Biological Pest Control ◽  
Root Knot Nematodes ◽  
Meloidogyne Spp ◽  
Trap Plants ◽  
Strong Inhibitory Effect

Root-knot nematodes (Meloidogyne spp.) are soil-borne pathogens that can cause severe damage to agricultural production. The most common approaches to prevent root-knot nematode infections are based on crop rotation with non-host plants, use of chemical insecticides, biological control methods, and use of nematode-antagonistic or trap plants. Marigolds (Tagetes erecta) are used as nematode-killing plants, but there is controversy over the mechanism through which they control root-knot nematodes. This study confirmed that marigold root-exudates are lethal to root-knot nematodes, illustrated that marigolds act as trap plants for root-knot nematodes when planted close to nematode host plants such as tomato. We investigated the rates of infection and development of nematode larvae injected into the marigold root system to evaluate whether marigolds could act as a non-host plant for root-knot nematodes. We found that aqueous solutions of marigold root-exudates showed strong lethal and inhibitory effects on sec-stage juveniles and eggs of root-knot nematodes. Marigold roots secreted substances that attracted nematodes from the surrounding environment. Furthermore, marigold root cells contained substances that had a strong inhibitory effect on the development of root-knot nematodes, resulting in diapause in nematodes, and inhibition of further infection. Herein we report a preliminary exploration of the antagonistic mechanism in marigolds for controlling the growth and development of root-knot nematodes. Our research provides basis for promoting the use of marigold for the control of nematodes as an important part of sustainable cropping strategies that rely on biological pest control. © 2021 Friends Science Publishers

scholarly journals Kök-Ur Nematodları (Meloidogyne spp.)’nda Parazitizm

2014 ◽  
Vol 2 (4) ◽  
pp. 160
Author(s):  
Gökhan Aydınlı ◽  
Sevilhan Mennan
Keyword(s):  
Molecular Basis ◽  
Host Response ◽  
Host Plants ◽  
Point Of View ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Management ◽  
Meloidogyne Spp ◽  
Nematode Parasitism ◽  
Complex Relationships

Root-knot nematodes (Meloidogyne spp.) have specialized and complex relationships with their host plants. A better understanding of interaction between nematode and their host will help to provide new point of view for root-knot nematode management. For this purpose, recently investigations on cellular and molecular basis of root-knot nematode parasitism and host response were reviewed.

scholarly journals The Use of Essential Oil and Hydrosol Extracted from Cuminum cyminum Seeds for the Control of Meloidogyne incognita and Meloidogyne javanica

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 46
Author(s):  
Iro Pardavella ◽  
Demetra Daferera ◽  
Theodoros Tselios ◽  
Panagiota Skiada ◽  
Ioannis Giannakou
Keyword(s):  
Essential Oil ◽  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Cuminum Cyminum ◽  
Clear Effect ◽  
Second Stage ◽  
Low Concentrations ◽  
Meloidogyne Species

The essential oil (EO) and hydrosol (HL) isolated from Cuminum cyminum (cumin) seeds were evaluated against the root-knot nematodes Meloidogyne incognita and M. javanica. The efficacy of extracts on the motility, hatching, and survival in soil of second-stage juveniles (J2s), and the activity on egg differentiation were tested. All J2s were paralyzed after immersion in the EO at 62.5 μL/L concentration for 96 h. Encouraging results were recorded using HL equal to or higher than 10% concentration for both Meloidogyne species tested. More than 70% paralyzed J2s were recorded after immersion for 48 h, while the percentage was increased to higher than 90% after 96 h of immersion. A clear effect on egg differentiation was observed after immersion in EO or HL. A significant decrease in egg differentiation was revealed at even low concentrations of EO while an evident decrease in egg differentiation was recorded after immersion of eggs in 50% HL dilution. Decreased hatching of M. incognita and M. javanica J2s was observed with the increase in concentration. The lowest numbers of hatched J2s were recorded when EO was used at 1000 and 2000 μL/L concentrations. A constant reduction in root-knot nematode J2 hatching was observed upon increasing the concentration of HL from 5% to 50%. The EO of C. cyminum is characterized by the presence of γ-terpinene-7-al (34.95%), cumin aldehydes (26.48), and α-terpinene-7-al (12.77%). The above constituents were observed in HL following the same order as that observed in EO. The components γ-terpinene (11.09%) and ο-cymene (6.56%) were also recorded in EO while they were absent in HL.

scholarly journals Members of the Meloidogyne Avirulence Protein Family Contain Multiple Plant Ligand-Like Motifs

2014 ◽  
Vol 104 (8) ◽  
pp. 879-885 ◽  
Author(s):  
William B. Rutter ◽  
Tarek Hewezi ◽  
Tom R. Maier ◽  
Melissa G. Mitchum ◽  
Eric L. Davis ◽  
...  
Keyword(s):  
Host Plants ◽  
Effector Proteins ◽  
Parasitic Nematodes ◽  
In Planta ◽  
Root Knot Nematode ◽  
Cyst Nematodes ◽  
Root Knot Nematodes ◽  
Cle Peptides ◽  
Variable Domains ◽  
Meloidogyne Spp

Sedentary plant-parasitic nematodes engage in complex interactions with their host plants by secreting effector proteins. Some effectors of both root-knot nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera and Globodera spp.) mimic plant ligand proteins. Most prominently, cyst nematodes secrete effectors that mimic plant CLAVATA3/ESR-related (CLE) ligand proteins. However, only cyst nematodes have been shown to secrete such effectors and to utilize CLE ligand mimicry in their interactions with host plants. Here, we document the presence of ligand-like motifs in bona fide root-knot nematode effectors that are most similar to CLE peptides from plants and cyst nematodes. We have identified multiple tandem CLE-like motifs conserved within the previously identified Meloidogyne avirulence protein (MAP) family that are secreted from root-knot nematodes and have been shown to function in planta. By searching all 12 MAP family members from multiple Meloidogyne spp., we identified 43 repetitive CLE-like motifs composing 14 unique variants. At least one CLE-like motif was conserved in each MAP family member. Furthermore, we documented the presence of other conserved sequences that resemble the variable domains described in Heterodera and Globodera CLE effectors. These findings document that root-knot nematodes appear to use CLE ligand mimicry and point toward a common host node targeted by two evolutionarily diverse groups of nematodes. As a consequence, it is likely that CLE signaling pathways are important in other phytonematode pathosystems as well.

Genetic diversity of Meloidogyne spp. parasitising potato in Brazil and aggressiveness of M. javanica populations on susceptible cultivars

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Israel L. Medina ◽  
Cesar B. Gomes ◽  
Valdir R. Correa ◽  
Vanessa S. Mattos ◽  
Philippe Castagnone-Sereno ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Potato Production ◽  
Potato Cultivars ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Reproduction ◽  
Meloidogyne Spp ◽  
Prevalent Species ◽  
Reproduction Factor

Root-knot nematodes (Meloidogyne spp.) significantly impact potato production worldwide and in Brazil they are considered one of the most important group of nematodes affecting potatoes. The objectives of this study were to survey Meloidogyne spp. associated with potatoes in Brazil, determine their genetic diversity and assess the aggressiveness of M. javanica on two susceptible potato cultivars. Fifty-seven root-knot nematode populations were identified using esterase phenotyping, including Meloidogyne javanica, M. incognita, M. arenaria and M. ethiopica. Overall, root-knot nematodes were present in ca 43% of sampled sites, in which M. javanica was the most prevalent species, and the phenotypes Est J3, J2a and J2 occurred in 91.2, 6.7 and 2.1% of the positive samples, respectively. Other species, such as M. incognita, M. arenaria and M. ethiopica, were found less frequently and occurred at rates of 6.4, 4.3 and 2.1% of the samples, respectively. Sometimes, M. javanica was found in mixtures with other root-knot nematodes in ca 10.6% of sites containing Meloidogyne. After confirming the identification of 17 isolates of M. javanica and one isolate each of M. incognita, M. arenaria and M. ethiopica by SCAR markers, the populations were used to infer their genetic diversity using RAPD markers. Results revealed low intraspecifc genetic diversity among isolates (13.9%) for M. javanica. Similarly, M. javanica sub-populations (J2a) clustered together (81% of bootstrap), indicating subtle variation from typical J3 populations. The aggressiveness of four populations of M. javanica from different Brazilian states on two susceptible potato cultivars was tested under glasshouse conditions. Results indicated differences in aggressiveness among these populations and showed that potato disease was proportional to nematode reproduction factor.

scholarly journals Heterologous Expression of the Mi-1.2 Gene from Tomato Confers Resistance Against Nematodes but Not Aphids in Eggplant

2006 ◽  
Vol 19 (4) ◽  
pp. 383-388 ◽  
Author(s):  
Fiona L. Goggin ◽  
Lingling Jia ◽  
Gowri Shah ◽  
Stephanie Hebert ◽  
Valerie M. Williamson ◽  
...  
Keyword(s):  
Genetic Background ◽  
Meloidogyne Javanica ◽  
Nematode Resistance ◽  
Macrosiphum Euphorbiae ◽  
Leucine Rich Repeat ◽  
Root Knot Nematode ◽  
Potato Aphid ◽  
Tomato Line ◽  
Meloidogyne Spp ◽  
Susceptible Tomato

The Mi-1.2 gene in tomato (Solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (NB-LRR) class of plant resistance genes, and confers resistance against root-knot nematodes (Meloidogyne spp.), the potato aphid (Macrosiphum euphorbiae), and the sweet potato whitefly (Bemisia tabaci). Mi-1.2 mediates a rapid local defensive response at the site of infection, although the signaling and defensive pathways required for resistance are largely unknown. In this study, eggplant (S. melongena) was transformed with Mi-1.2 to determine whether this gene can function in a genetic background other than tomato. Eggplants that carried Mi-1.2 displayed resistance to the root-knot nematode Meloidogyne javanica but were fully susceptible to the potato aphid, whereas a susceptible tomato line transformed with the same transgene was resistant to nematodes and aphids. This study shows that Mi-1.2 can confer nematode resistance in another Solanaceous species. It also indicates that the requirements for Mi-mediated aphid and nematode resistance differ. Potentially, aphid resistance requires additional genes that are not conserved between tomato and eggplant.

scholarly journals Differences in Feeding Sites Induced by Root-Knot Nematodes, Meloidogyne spp., in Chickpea

2005 ◽  
Vol 95 (4) ◽  
pp. 368-375 ◽  
Author(s):  
Nicola Vovlas ◽  
Hava F. Rapoport ◽  
Rafael M. Jiménez Díaz ◽  
Pablo Castillo
Keyword(s):  
Giant Cell ◽  
Faba Bean ◽  
Nematode Species ◽  
Giant Cells ◽  
The Other ◽  
Root Knot Nematodes ◽  
Feeding Site ◽  
Feeding Sites ◽  
Meloidogyne Spp ◽  
Meloidogyne Species

Root-knot nematodes (Meloidogyne spp.) are sedentary, obligate endoparasites in plants, where they induce specialized feeding sites. The feeding sites act as strong metabolic sinks to which photosynthates are mobilized. The histopathological modifications in the nematode-induced feeding sites of artificially inoculated chickpea cv. UC 27 were qualitatively and quantitatively compared using five isolates of M. artiellia and one isolate each of M. arenaria, M. incognita, and M. javanica. All Meloidogyne isolates infected chickpea plants, but root gall thickening was significantly less for M. artiellia isolates than for the other Meloidogyne species. Nevertheless, neither the number of giant cells in the feeding site (averaging four to six) nor the area of individual giant cells was influenced by nematode species or isolate. However, the number of nuclei per giant cell was significantly smaller, and the maximum diameters of nuclei and nucleoli were significantly greater, in giant cells induced by M. artiellia isolates than in those induced by M. arenaria, M. incognita, or M. javanica. In a second experiment, M. artiellia-induced giant cells in faba bean and rapeseed also contained a small number of large nuclei.

scholarly journals Host plants of root-knot nematodes

1975 ◽  
Vol 32 (0) ◽  
pp. 527-530
Author(s):  
Luiz Gonzaga E. Lordello ◽  
Luiz Carlos Fazuoli ◽  
Condorcet Aranha ◽  
Rubens R.A. Lordello
Keyword(s):  
Coffea Arabica ◽  
Host Plants ◽  
Coffea Canephora ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Digitaria Sanguinalis ◽  
Bidens Pilosa ◽  
Root Knot Nematodes ◽  
Eleusine Indica ◽  
Solanum Americanum

Root-knot nematodes were found attacking Coffea spp. and also roots of a few weed species usually found in the coffee orchards in São Paulo. C. arabica cv. Catuaí, C. arabica cv. Mundo Novo, Timor Hybrid and a few plants of C. racemosa showed to be susceptible to Meloidogyne exigua. Roots of Ageratum conyzoides, Amaranthus viridis, Bidens pilosa, Coffea arabica cv. Mundo Novo, Coffea racemosa, Commelina virginica, Digitaria sanguinalis, Galinsoga parviflora, Gnaphalium spathulatum, Porophyllum ruderale, Portulaca oleracea, Pterocaulon virgatum and Solanum americanum were disfigured by M. incognita M. arenaria was found attacking roots of Eleusine indica and Gnaphalium spathulatum, and the presence of an unidentified Meloidogyne species was verified in roots of the following species: Vernonia ferruginea, C. arabica x C. canephora, Eupatorium pauciflorum, Coffea canephora cv. Kouillou, Coffea eugenioides, Coffea racemosa, Coffea stenophylla, Euphorbia pilullifera, Solanum americanum, Ageratum conyzoides, Phyllanthus corcovadensis, and Emilia sagittata.

scholarly journals First Report of the Root-Knot Nematode Meloidogyne floridensis on Tomato (Lycopersicon esculentum) in Florida

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 527-527
Author(s):  
G. T. Church
Keyword(s):  
Lycopersicon Esculentum ◽  
Field Experiments ◽  
The United States ◽  
The State ◽  
Bromophenol Blue ◽  
Root Knot Nematode ◽  
Fresh Market ◽  
Root Knot Nematodes ◽  
First Report ◽  
Meloidogyne Spp

The state of Florida is the largest producer of fresh market tomato (Lycopersicon esculentum L.) in the United States with 2003 yields of 634 million kg on 17,700 ha valued at 516 million dollars. Effective crop management is essential for production of vegetables in Florida because of the presence of intense pest pressure. The identification of the pests present is the first step in the development of a successful IPM (integrated pest management) program. Root-knot nematodes (Meloidogyne spp.) are common nematodes that parasitize vegetables in Florida and cause significant yield reductions when not properly managed. In 2003 field experiments, soil was collected from two research farms in Saint Lucie and Seminole counties in Florida. Galling caused by root-knot nematode was observed on tomato at both locations. Since females suitable for identification are difficult to obtain from field-grown roots, field soil was placed in pots in the greenhouse and planted with Lycopersicon esculentum cv. Rutgers. Standard morphological techniques, differential host tests, and isozyme phenotypes were used in nematode identification. Female root-knot nematodes were extracted from tomato roots and placed in extraction buffer (10% wt/vol sucrose, 2% vol/vol Triton X-100, 0.01% wt/vol bromophenol blue). The females were crushed, loaded on a polyacrylamide gel, and separated by electrophoresis using the PhastSystem (Amersham Biosciences, Piscataway, NJ). The activities of malate dehydrogenase and esterase enzymes were detected using standard techniques. Isozyme phenotypes consistent with Meloidogyne incognita (Kofoid and White) Chitwood and M. javanica (Treub) Chitwood as well as with the newly described M. floridensis Handoo (1) were observed at both locations. To our knowledge, this is the first report of M. floridensis naturally occurring on tomato in Florida. The identification and distribution of M. floridensis in vegetable production fields is important for disease management throughout the state since the host range is likely different from other Meloidogyne spp. Reference: (1) Z. A. Handoo et al. J. Nematol. 36:20, 2004.

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