Genome-Wide Identification of TIFY Genes and Their Response to Various Pathogen Infections in Cucumber (Cucumis sativus L.)

2022 ◽  
Vol 295 ◽  
pp. 110814
Author(s):  
Zhuonan Dai ◽  
Shaoyun Dong ◽  
Han Miao ◽  
Xiaoping Liu ◽  
Jianan Han ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Cucumis Sativus L ◽  
Genome Wide

scholarly journals Genome-wide analysis of valine-glutamine motif-containing proteins related to abiotic stress response in cucumber (Cucumis sativus L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nan Shan ◽  
Zijin Xiang ◽  
Jingyu Sun ◽  
Qianglong Zhu ◽  
Yao Xiao ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Genetic Basis ◽  
Resistance Breeding ◽  
Abiotic Stress Response ◽  
Phylogenetic Tree Analysis ◽  
Cucumis Sativus L ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Specific Proteins ◽  
Spatio Temporal

Abstract Background Cucumber (Cucumis sativus L.) is one of the most important economic crops and is susceptible to various abiotic stresses. The valine-glutamine (VQ) motif-containing proteins are plant-specific proteins with a conserved “FxxhVQxhTG” amino acid sequence that regulates plant growth and development. However, little is known about the function of VQ proteins in cucumber. Results In this study, a total of 32 CsVQ proteins from cucumber were confirmed and characterized using comprehensive genome-wide analysis, and they all contain a conserved motif with 10 variations. Phylogenetic tree analysis revealed that these CsVQ proteins were classified into nine groups by comparing the CsVQ proteins with those of Arabidopsis thaliana, melon and rice. CsVQ genes were distributed on seven chromosomes. Most of these genes were predicted to be localized in the nucleus. In addition, cis-elements in response to different stresses and hormones were observed in the promoters of the CsVQ genes. A network of CsVQ proteins interacting with WRKY transcription factors (CsWRKYs) was proposed. Moreover, the transcripts of CsVQ gene were spatio-temporal specific and were induced by abiotic adversities. CsVQ4, CsVQ6, CsVQ16–2, CsVQ19, CsVQ24, CsVQ30, CsVQ32, CsVQ33, and CsVQ34 were expressed in the range of organs and tissues at higher levels and could respond to multiple hormones and different stresses, indicating that these genes were involved in the response to stimuli. Conclusions Together, our results reveal novel VQ resistance gene resources, and provide critical information on CsVQ genes and their encoded proteins, which supplies important genetic basis for VQ resistance breeding of cucumber plants.

scholarly journals Genome-wide identification and expression analysis of CYP450 gene family in Cucumis sativus L.

2021 ◽  
Author(s):  
hongyu wang ◽  
Pengfei Li ◽  
Yu Wang ◽  
Chunyu Chi ◽  
Guohua Ding
Keyword(s):  
Subcellular Localization ◽  
Gene Family ◽  
Cucumis Sativus ◽  
Expression Profile ◽  
Evolutionary Relationship ◽  
Evolutionary Analysis ◽  
Cucumis Sativus L ◽  
P450 Gene ◽  
Genome Wide ◽  
A Genome

Abstract The cytochrome P450 (CYP450) gene family plays a vital role in basic metabolism and enhances plant resistance to stress and pests. However, little information is available on the genome-wide characterization and evolutionary relationship of the CYP450 gene family in Cucumis sativus L. In the present study, a genome-wide bioinformatics analysis was performed, including gene structure, conserved motif, cis-acting promoter element, evolutionary analysis, collinearity, subcellular localization, and expression profile. The gene expression profile of CYP450 was verified using transcriptome sequencing and quantitative reverse transcription polymerase chain reaction. A total of 165 P450 genes were identified in the cucumber genome. These genes were classified into eight subfamilies and unevenly distributed on seven chromosomes. Subcellular localization predicted that most of P450 genes were located in chloroplasts and a few were located on the plasma membrane. CYP450 genes were differentially expressed in different tissues and in response to salicylic acid (SA) treatment. The sizes of all cucumber P450 proteins ranged from 317 to 1,056 aa, the theoretical isoelectric points ranged from 5.05 to 10.31, and the molecular weights ranged from 36,095 to 121,403 KD. This study provides a theoretical basis for further research on the biological functions of the P450 gene in cucumber plants.

scholarly journals Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)

BMC Genomics ◽  
2010 ◽  
Vol 11 (1) ◽  
Author(s):  
Pablo F Cavagnaro ◽  
Douglas A Senalik ◽  
Luming Yang ◽  
Philipp W Simon ◽  
Timothy T Harkins ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Simple Sequence Repeats ◽  
Cucumis Sativus L ◽  
Genome Wide ◽  
Simple Sequence

scholarly journals Genome-wide analysis of OVATE family proteins in cucumber (Cucumis sativus L.)

2019 ◽  
Author(s):  
Lijie Han ◽  
Xiaofei Song ◽  
Zhongyi Wang ◽  
Xiaofeng Liu ◽  
Liying Yan ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Reproductive Organs ◽  
Cucumis Sativus L ◽  
Hormone Response Elements ◽  
Genome Wide ◽  
Cucumber Fruit ◽  
Specific Proteins ◽  
Gibberellin Treatment

Abstract Background: OVATE family proteins (OFPs) are plant-specific proteins with the conserved OVATE domain that regulating plant growth and development. Although these OFPs have been studied in several species, the biological functions of this OFP gene family remain largely unknown in cucumber (Cucumis sativus L.). Results: In this study, we identified 19 CsOFPs in cucumber. This CsOFPs are distributed on seven chromosomes and can be divided into four subgroups. Most CsOFP genes are expressed in reproductive organs although have different expression patterns. Cis-elements analysis showed that there are six kinds of hormone response elements in CsOFPs and exogenous gibberellin treatment leads to a ‘first increase then decrease’ expression pattern of CsOFP7, CsOFP11 and CsOFP12. Ectopic expression of CsOFP11 in Arabidopsis resulted in shorter and blunt siliques. Conclusions: Together, these results indicated that CsOFPs may play important roles in cucumber fruit development.

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