Potential threat of antibiotics resistance genes in bioleaching of heavy metals from sediment

Abstract Baiyangdian Lake is a typical and largest multi-habitat lake in the North plain of China. To understand the generation and transmission of antibiotics resistance genes (ARGs) in multi-habitat lakes, the contents of nutrients (TC, TOC, TN, TP and TS), heavy metals (Zn, Cr, Ni, Cu, Pb, As, Cd and Hg), 22 antibiotics, 16S-rRNA(16S), Class I integron (intI1) and 20 ARGs were determined. Samples were taken from the Fuhe river, river estuaries, reed marshes, living area, fish poods and open water of Baiyangdian Lake. The results showed that quinolones (QNs) were the main pollutants, and the content range was ND-104.94 ng/g. Thereinto, aac (6') -IB, blaTEM-1, ermF, qnrA, qnrD, tetG, sul1, sul2 and tetM were detected in 100%. The absolute abundance of sul1 was the highest (5.25×105copies/g-6.21×107 copies/g), which was the dominant ARGs. In these different habitats, the abundance of antibiotics and ARGs in river estuary was the highest, and that in reed marshes was the lowest. There was a significant positive correlation between the abundance of heavy metals (Cu, Pb, Zn, Ni, Cd, Hg) and the absolute abundance of 11 ARGs (P<0.01). Redundancy analysis showed that Cu, Zn, intI1, TP and macrolides (MLs) were the important factors affecting the distribution of ARGs. Our findings provides a more likely driving and influencing factor for the transmission of ARGs in lakes with complex and diverse habitats.

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A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils

International Journal of Molecular Sciences ◽

10.3390/ijms22073438 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3438

Author(s):

Juan Liu ◽

Xiangwei He ◽

Jingya Sun ◽

Yuchao Ma

Keyword(s):

Soil Fertility ◽

Microbial Diversity ◽

Resistance Genes ◽

Bacterial Communities ◽

Rhizosphere Soil ◽

Antibiotics Resistance ◽

Plant Growth Promoting Bacteria ◽

Soil Microbial Diversity ◽

Antibiotic Target ◽

And Function

Bacterial communities associated with roots influence the health and nutrition of the host plant. However, the microbiome discrepancy are not well understood under different healthy conditions. Here, we tested the hypothesis that rhizosphere soil microbial diversity and function varies along a degeneration gradient of poplar, with a focus on plant growth promoting bacteria (PGPB) and antibiotic resistance genes. Comprehensive metagenomic analysis including taxonomic investigation, functional detection, and ARG (antibiotics resistance genes) annotation revealed that available potassium (AK) was correlated with microbial diversity and function. We proposed several microbes, Bradyrhizobium, Sphingomonas, Mesorhizobium, Nocardioides, Variovorax, Gemmatimonadetes, Rhizobacter, Pedosphaera, Candidatus Solibacter, Acidobacterium, and Phenylobacterium, as candidates to reflect the soil fertility and the plant health. The highest abundance of multidrug resistance genes and the four mainly microbial resistance mechanisms (antibiotic efflux, antibiotic target protection, antibiotic target alteration, and antibiotic target replacement) in healthy poplar rhizosphere, corroborated the relationship between soil fertility and microbial activity. This result suggested that healthy rhizosphere soil harbored microbes with a higher capacity and had more complex microbial interaction network to promote plant growing and reduce intracellular levels of antibiotics. Our findings suggested a correlation between the plant degeneration gradient and bacterial communities, and provided insight into the role of high-turnover microbial communities as well as potential PGPB as real-time indicators of forestry soil quality, and demonstrated the inner interaction contributed by the bacterial communities.

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Ryegrass (Lolium multiflorum L.) and Indian mustard (Brassica juncea L.) intercropping can improve the phytoremediation of antibiotics and antibiotic resistance genes but not heavy metals

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.147093 ◽

2021 ◽

pp. 147093

Author(s):

Erping Cui ◽

Bingjian Cui ◽

Xiangyang Fan ◽

Songjing Li ◽

Feng Gao

Keyword(s):

Heavy Metals ◽

Antibiotic Resistance ◽

Brassica Juncea ◽

Resistance Genes ◽

Lolium Multiflorum ◽

Indian Mustard ◽

Antibiotic Resistance Genes

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Occurrence of heavy metals, antibiotics, and antibiotic resistance genes in different kinds of land-applied manure in China

Environmental Science and Pollution Research ◽

10.1007/s11356-021-13307-9 ◽

2021 ◽

Author(s):

Jiajia Xue ◽

Jianqiang Wu ◽

Yaru Hu ◽

Chenyan Sha ◽

Shijie Yao ◽

...

Keyword(s):

Heavy Metals ◽

Antibiotic Resistance ◽

Resistance Genes ◽

Antibiotic Resistance Genes

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Effects of humic acid on sludge performance, antibiotics resistance genes propagation and functional genes expression during Cu(II)-containing wastewater treatment via metagenomics analysis

Bioresource Technology ◽

10.1016/j.biortech.2020.124575 ◽

2021 ◽

Vol 323 ◽

pp. 124575

Author(s):

Jianguo Zhao ◽

Yu Li ◽

Yahe Li ◽

Ke Zhang ◽

Hongzhong Zhang ◽

...

Keyword(s):

Wastewater Treatment ◽

Humic Acid ◽

Resistance Genes ◽

Functional Genes ◽

Antibiotics Resistance ◽

Genes Expression ◽

Metagenomics Analysis

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Screening of heavy metal and antibiotic resistance among Proteus vulgaris isolates from hospital wastewater of Northern India

Anti-Infective Agents ◽

10.2174/2211352519666210308101621 ◽

2021 ◽

Vol 19 ◽

Author(s):

Manzar Alam ◽

Mohd Imran ◽

Syed Sayeed Ahmad

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Multiple Drug Resistance ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Antibiotics Resistance ◽

Proteus Vulgaris ◽

Resistance To Antibiotics ◽

Transfer Frequency ◽

Resistance Patterns

Background: Microbial resistance to antibiotics and heavy metals is a rising problem in the world today. All the Proteus vulgaris isolates showed their MIC in between 50-1600 µg/ml. Of 70% and 46% of the isolates showed their MIC at 800-1200 µg/ml against Zn2+ and Cu2+ while 80% of the isolates showed their MIC at 100-200 µg/ml against Ni2+, respectively. All Proteus vulgaris isolates also exhibited multiple resistance patterns (2-7 heavy metals) in different combination of metals. The Multi metal resistance Index (MHMR) ranges were found (0.04-0.5). Methods: A high level of antibiotics resistance was observed against Methicillin (100%) and least to Oflaxicin (6%), Gentamycine and Neomycin (10%). All Proteus vulgaris isolates also showed multiple drug resistance patterns (2-12 antibiotics) in different combination of antibiotics. The MAR index ranges were found (0.02-0.7). Of 98%, 84% and 80% of the total isolates showed urease, gelatinase and amylase activity. Results: The Proteus vulgaris isolates contained plasmid of size ranging from 42.5 to 57.0kb and molecular weight of plasmids ranged from 27.2 to 37.0 MD. Incidences of resistance transfer, 7 pairs of isolates were assessed for the transfer of the antibiotic/ heavy metal resistance markers. The higher (4.4x10-1 and 3.4x10-1) transfer frequency was observed among antibiotic and heavy metal while lower transfer frequency were (5.0x10-2 and 1.0x10-2) showed against antibiotic and heavy metal in both the medium from the entire site tested, respectively. Conclusion: Indicating the high threat of environmental pollution and appearance of heavy metal resistance which may support the enlargement of resistance to antibiotics among the pathogens.

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Seasonal Variation And Preliminary Risk Assessment Of Trace Element Pollution In Surface Water From Langat River, Malaysia

10.31219/osf.io/zj4f6 ◽

2019 ◽

Author(s):

Lubna Alam ◽

Md. Mahmudul Alam ◽

Mazlin Bin Mokhtar ◽

Azizul Bar ◽

Nicholas Kathijotes ◽

...

Keyword(s):

Heavy Metals ◽

Seasonal Variation ◽

River Water ◽

Critical Index ◽

Principal Component ◽

Dermal Absorption ◽

Potential Threat ◽

Concentration Data ◽

Trace Element Pollution ◽

Langat River

Heavy metals are widely used in various industries and became a great concern all over the world due to environmental contamination. This study provides an assessment of seasonal variability and risks to human health associated with the exposure to heavy metals concentrated in Langat river water of Malaysia. The Department of Environment (DOE) Malaysia kindly provided the heavy metal concentration data in water for this study. Several multivariate estimation such as an independent t test, box-and-whisker plot and Principal component analysis were carried out to evaluate the seasonal variation of metals concentration in water. The average value of ten analyzed metals was 250.81 µg/l and followed in order of abundance by August > Jun > February > October > April > December > March > May > September > January > July > November. The calculated HPI was 123.42, which is far above the critical index value of 100, indicating pollution with respect to heavy metals. Estimates of health risks associated with river water were summarized according to non-carcinogenic and carcinogenic health effects. No potential threat was detected for noncarcinogenic risk as the HI values calculated were <1. Potential carcinogenic risks associated with the ingestion and dermal absorption of heavy metals in water were evaluated probabilistically by performing 10,000 trails for Monte Carlo simulation where potential carcinogenic risk exists in case of Cd and As.It is necessary to take proper steps to reduce the pollution of heavy metals in Langat River.

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Heavy Metal Tolerance Bacillus spp Isolated From Crude Oil Polluted Soil

JOURNAL OF RESEARCH AND REVIEW IN SCIENCE ◽

10.36108/jrrslasu/0202.70.0180 ◽

2020 ◽

Vol 7 (1) ◽

Author(s):

Muibat Fashola

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Crude Oil ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Polluted Soil ◽

Diffusion Method ◽

Bacillus Species ◽

Antibiotics Resistance ◽

Bacillus Spp

Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase in heavy metals load in the soil. Heavy metals exert toxic effects on biodegradation of organic pollutant in cocontaminated soil and there is need to find suitable strategies for their removal. Aim: The aim of this study was to assess the heavy metals resistance capability of indigenous Bacillus species in hydrocarbon polluted soil to nickel (Ni), Cadmium (Cd), Lead (Pb) and Chromium (Cr). Materials and Methods: Heavy metal tolerant bacteria were isolated from hydrocarbon polluted soil using Luria-Berthani agar supplemented with the respective metals and spread plate techniques. The isolates were putatively identified on the basis of their colonial morphology and biochemical characteristics and their antibiotics susceptibility pattern were evaluated using disc diffusion method. Results: The maximum tolerable concentration (MTC) of the four heavy metals to the selected isolates was 2 mM. Four bacteria isolates able to withstand the MTC were putatively identified as Bacillus subtilis, Bacillus megaterium, Bacillus laterosporus and Bacillus polymyxa. Out of the four Bacillus species, only B. laterosporus did not show multiple tolerance to the tested antibiotics which show that there is correlation between heavy metal tolerance and antibiotics resistance by the isolates. Conclusion: Multiple heavy metal tolerance Bacillus spp. were isolated from crude oil polluted soil. These bacteria could be suitable agents for bioaugmentation of hydrocarbon polluted soil co-contaminated with heavy metals.

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Health Risk Assessment of Heavy Metals in Traditional Cosmetics Sold in Tunisian Local Markets

International Scholarly Research Notices ◽

10.1155/2016/6296458 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 8

Author(s):

Mohamed Anouar Nouioui ◽

Salah Mahjoubi ◽

Asma Ghorbel ◽

Marouen Ben Haj Yahia ◽

Dorra Amira ◽

...

Keyword(s):

Heavy Metals ◽

Risk Assessment ◽

Heavy Metal ◽

Health Risk ◽

Atomic Absorption ◽

Good Manufacturing Practice ◽

Potential Threat ◽

Analytical Test ◽

Metal Contents ◽

Cu And Zn

This study was undertaken in order to determine heavy metal contents in twelve (n=12) henna brands and eleven (n=11) kohl products. An analytical test was performed for Pb, Cd, Cu, and Zn in henna and kohl products using atomic absorption spectrophotometery. The overall mean concentrations of heavy metals in henna varied between 1.2 and 8.9 μg g−1 for Pb; 0.8 and 18.6 μg g−1 for Cd; 0.5 μg g−1 and 3.3 μg g−1 for Cu; and 3.7 μg g−1 and 90.0 μg g−1 for Zn. As for kohl products, Pb concentrations ranged between 51.1 μg g−1 and 4839.5 μg g−1, Cd concentrations ranged between 1.0 μg g−1 and 158.6 μg g−1, Cu concentrations ranged between 2.5 μg g−1 and 162.5 μg g−1, and Zn concentrations ranged between 0.7 μg g−1 and 185.0 μg g−1. The results of our study revealed that Pb, Cd, Cu, and Zn contents in investigated samples were high, making from the prolonged use of such products a potential threat to human health. Therefore, major quality controls are recommended in order to enforce acceptable limits of potential contaminants in cosmetics and good manufacturing practice.

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