Recovery of liquiritin and glycyrrhizic acid from Glycyrrhiza uralensis Fisch by aqueous two-phase flotation and multi-stage preparative high performance liquid chromatography

2014 ◽  
Vol 134 ◽  
pp. 204-209 ◽  
Author(s):  
Lin Chang ◽  
Yun Wei ◽  
Peng-yu Bi ◽  
Qian Shao
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycyrrhizic Acid ◽  
Glycyrrhiza Uralensis ◽  
Two Phase ◽  
Glycyrrhiza Uralensis Fisch ◽  
Multi Stage

scholarly journals Simultaneous Quantitative Analysis of Q-Marker with One Single Reference in Glycyrrhiza uralensis Fisch

2020 ◽  
Vol 58 (6) ◽  
pp. 511-519
Author(s):  
Xin Dong ◽  
Fangyuan Zheng ◽  
Xin Liu ◽  
Lianju Zhang ◽  
Rongqin Hu ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Standard Method ◽  
High Performance ◽  
Glycyrrhiza Uralensis ◽  
Glycyrrhiza Uralensis Fisch ◽  
External Standard Method ◽  
Relative Standard

Abstract In traditional Chinese medicine (TCM) studies, it is difficult to choose evaluation markers for the strict quality control of herbs. A high performance liquid chromatography coupled with metabolomics for simultaneous quantitative analysis of quality markers (Q-markers) in Glycyrrhiza uralensis Fisch was established, which could not only ensure the quality and batch-to-batch consistency of TCMs, but also achieve a quantitative analysis of multi-components by the single reference standard. Based on the construction of chromatographic profiles by high performance liquid chromatography (HPLC) and HPLC-Q-Exactive/MS methods, different multivariate analyses were employed. Seven quantitative indices were selected as the Q-markers, and a reliable quantification method was established. The quantitative method was acceptable with good linearity with correlation coefficients >0.9993 and satisfactory repeatability (relative standard deviation (RSD) < 0.05%), precision (RSD < 0.24%), reproducibility (RSD < 0.97%), stability (RSD < 2.52%) and recoveries (96.96%—98.52%, RSD < 3.24%), and no significant differences were observed between the external standard method and the new method as determined by calculating standard method difference. Overall, the study suggests that the simultaneous quantitative analysis of main Q-marker in G. uralensis Fisch with one single marker can be considered good quality criteria for performing quality control of G. uralensis Fisch.

Compositional Analysis of Organosolv Poplar Lignin by Using High-Performance Liquid Chromatography/High-resolution Multi-stage Tandem Mass Spectrometry

2021 ◽  
Author(s):  
Jifa Zhang ◽  
Yuan Jiang ◽  
Leah F Easterling ◽  
Anton Anster ◽  
Wanru Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Compositional Analysis ◽  
Complex Mixtures ◽  
Tandem Mass ◽  
Multi Stage ◽  
The Individual ◽  
Environmentally Friendly Process

Organosolv treatment is an efficient and environmentally friendly process to degrade lignin into small compounds. The capability of characterizing the individual compounds in the complex mixtures formed upon organosolv treatment...

scholarly journals Estimation of Some Useful Drugs in Water, Urine, and Medical Formulations Following Conventional and Modified Dispersive Microextraction Coupled with HPLC-UV

2021 ◽  
pp. 11-33
Author(s):  
Mitali Sarkar ◽  
Shanku Denrah
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Extraction Efficiency ◽  
Sodium Dodecyl ◽  
Two Phase ◽  
Preconcentration Factor ◽  
Dispersive Microextraction ◽  
Dispersive Liquid Liquid Microextraction ◽  
Thermodynamic Feasibility

Dispersive Liquid-Liquid Microextraction (DLLME) coupled with high-performance liquid chromatography-ultraviolet spectroscopy was developed, as a fast and precise operation, for extractive recovery and estimation of two pharmaceuticals viz. moxifloxacin and galantamine, from water, urine, and medical formulations. The process was investigated for Extraction (ES) and Dispersive Solvent (DS) as well as pH, temperature, and salt concentration. Extraction was found effective using methanol (CH3OH), as the DS, employing 1,1,2,2-tetrachloroethane (C2H2Cl4) and chloroform (CHCl3), as the ES, for moxifloxacin and galantamine respectively. The optimum pH was found to be 6.9 for moxifloxacin and 10.2 for galantamine. Temperature and salt were found to have some influence on the extraction efficiency of moxifloxacin but insignificant for galantamine. An improvement of the operation in terms of the Extraction efficiency (ER %), Preconcentration Factor (PF), thermodynamic feasibility, and greenness were achieved during surfactant aided DLLME (SDS-DLLME), where anionic surfactant (Sodium Dodecyl Sulphate (SDS)) was employed and no DS was required. Interestingly, the volume requirement for ES was found less, compared to that in the conventional DLLME, without compromising the performance. Moreover, quantitative recovery of both the drugs was achieved using a single ES. Thus, mutual separation and simultaneous determination of moxifloxacin and galantamine may be designed. A two-phase separation with concomitant enrichment of the solute in the sediment phase occurred. The drugs in the sediment phase, on subsequent dilution with methanol, were determined using the High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) system. The negative free energy changes for the operation indicated that the process was thermodynamically feasible. The process was found to be effective for the spiked recovery of the studied drugs from real samples viz, water, human urine, and commercial medical formulations.

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