scholarly journals Simultaneous Quantitative Analysis of Q-Marker with One Single Reference in Glycyrrhiza uralensis Fisch

2020 ◽  
Vol 58 (6) ◽  
pp. 511-519
Author(s):  
Xin Dong ◽  
Fangyuan Zheng ◽  
Xin Liu ◽  
Lianju Zhang ◽  
Rongqin Hu ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Standard Method ◽  
High Performance ◽  
Glycyrrhiza Uralensis ◽  
Glycyrrhiza Uralensis Fisch ◽  
External Standard Method ◽  
Relative Standard

Abstract In traditional Chinese medicine (TCM) studies, it is difficult to choose evaluation markers for the strict quality control of herbs. A high performance liquid chromatography coupled with metabolomics for simultaneous quantitative analysis of quality markers (Q-markers) in Glycyrrhiza uralensis Fisch was established, which could not only ensure the quality and batch-to-batch consistency of TCMs, but also achieve a quantitative analysis of multi-components by the single reference standard. Based on the construction of chromatographic profiles by high performance liquid chromatography (HPLC) and HPLC-Q-Exactive/MS methods, different multivariate analyses were employed. Seven quantitative indices were selected as the Q-markers, and a reliable quantification method was established. The quantitative method was acceptable with good linearity with correlation coefficients >0.9993 and satisfactory repeatability (relative standard deviation (RSD) < 0.05%), precision (RSD < 0.24%), reproducibility (RSD < 0.97%), stability (RSD < 2.52%) and recoveries (96.96%—98.52%, RSD < 3.24%), and no significant differences were observed between the external standard method and the new method as determined by calculating standard method difference. Overall, the study suggests that the simultaneous quantitative analysis of main Q-marker in G. uralensis Fisch with one single marker can be considered good quality criteria for performing quality control of G. uralensis Fisch.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Simultaneous Quantification of Two Flavonoids in Morus alba by High Performance Liquid Chromatography Coupled with Photodiode Array Detector

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Morus Alba ◽  
Array Detector ◽  
Root Bark ◽  
Relative Standard ◽  
Photodiode Array

The root bark of Morus alba L. (Family: Moraceae) is an important medicinal herb in many countries and has long been used as a traditional medicine for the treatment of cough, fever, blood pressure reduction, and respiratory diseases. In the present study, the simultaneous determination of two flavonoids, kuwanon G and morusin, for quality control of M alba was conducted using high-performance liquid chromatography (HPLC) equipped with photodiode array (PDA) detector. The column used for separation of kuwanon G and morusin was a Gemini C18 analytical column maintained at 45°C. The mobile phase for efficient separation of two analytes was flowed 0.1% (v/v) aqueous formic acid-acetonitrile with gradient elution. The detection wavelength for quantification was set at 266 nm. The optimized method showed good linearity with coefficients of determination of 0.9998 within the tested concentration ranges. The limits of detection for the two flavonoids, kuwanon G and morusin, were 0.69 μg/mL and 0.35 μg/mL and the limits of quantification of kuwanon G and morusin, were 2.10 μ/mL and 1.07 μg/mL. The recoveries were 98.40–111.55% and the relative standard deviation (RSD) value was within 3.50%. The RSD values of intra- a g d interday precisions were 0.08–0.70% and 0.06-0.48%, respectively. The amounts of kuwanon G and morusin were 1.94-2.26 mg/g and 1.05–1.12 mg/g. The established HPLC-PDA method will help to improve the quality control of M. alba and related products.

Determination of Nitrofurans Residue in Fishery Products Using High Performance Liquid Chromatography

2012 ◽  
Vol 554-556 ◽  
pp. 1013-1016 ◽  
Author(s):  
Xuan Yun Huang ◽  
Dong Mei Huang ◽  
Yong Fu Shi ◽  
Bing Feng ◽  
Bing Li ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Correlation Coefficients ◽  
Hplc Method ◽  
Commercial Fishery ◽  
External Standard Method ◽  
Relative Standard ◽  
Normal Hexane ◽  
Fishery Products

Nitrofurans residue in fishery products were dangerous for human health and still remain a big challenge in China. However, there are a few available methods to detect nitrofurans residue in fishery products. This study aimed at developing a high performance liquid chromatography (HPLC) method to detect four nitrofurans (furaltadone, nitrofurazone, nitrofurantoin and furazolidone) residue in fishery products sensitively . Firstly the nitrofurans were extracted from samples with ethyl acetate and then concentrated with rotary evaporator. After mobile phase dissolved and normal hexane defatted, the analytes were quantified with an external standard method by HPLC. The results showed that the linear range was 0.025~1.000μg/mL with correlation coefficients all more than 0.998 and the quantification limits of furaltadone, nitrofurazone, nitrofurantoin, furazolidone, were 11.0, 6.00, 13.0 and 7.00μg/kg respectively. The method was used to detect the nitrofurans in fishery products prepared with recovery rates from 72.2% to 107.4% and relative standard deviation (RSD) varied from 6.2% to 10.4%. Therefore, this method can be used for monitoring nitrofurans in commercial fishery products, improving the safety of commercial fishery products in China.

scholarly journals Qualitative and Quantitative Analysis of the Major Constituents in WLJ Herbal Tea Using Multiple Chromatographic Techniques

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2623 ◽  
Author(s):  
Chao-Zhan Lin ◽  
Run-Jing Zhang ◽  
Yu-Feng Yao ◽  
Xiao-Dan Huang ◽  
Rong-Bo Zheng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Good Precision ◽  
Herbal Tea ◽  
Chromatographic Techniques ◽  
Flight Mass Spectrometry

Quality control of Chinese herbal tea remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling and famous brand of beverage in China, Wanglaoji Herbal Tea (WLJHT), via a full component quantitative analysis. In this paper, a total of thirty-two representative constituents were identified or tentatively characterized using ultra-high performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Moreover, the quantitative analyses of fourteen constituents were performed by high performance liquid chromatography with a triple quadruple tandem mass spectrometry (HPLC-MS/MS) method and saccharide compositions of WLJHT were also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on a Hilic column, separately. Using multiple chromatographic techniques presented a good precision, sensitivity, repeatability and stability, and was successfully applied to analyze 16 batches of WLJHT samples. Therefore, it would be a reliable and useful approach for the quality control of WLJHT.

scholarly journals Determination of two acrylates in environmental water by high performance liquid chromatography

2021 ◽  
Vol 233 ◽  
pp. 01023
Author(s):  
Yue Qiu ◽  
Qing Zhang ◽  
Genrong Li ◽  
Yingkun Gong ◽  
Mei Long
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Environmental Water ◽  
Hplc Method ◽  
Column Temperature ◽  
Standard Curve ◽  
External Standard Method ◽  
Relative Standard

A high performance liquid chromatography (HPLC) method was established for the determination of two acrylate substances in environmental water. The optimal chromatographic conditions were determined via exploring the effects of chromatographic column, mobile phase, column temperature, flow rate, detection wavelength and other factors on the separation effect of acrylate substances. Finally, the effective separation of methyl methacrylate and isopropyl methacrylate was realized within 6 min. The retention time of the target compound was used for qualitative analysis and the external standard method was used for quantitative analysis in the experiment. The linear relationship between the two acrylates was good in the range of 0.2-50.0 mg/L, and the correlation coefficient of standard curve was higher than 0.999. The recovery rate was 88.6%-105.3%, the relative standard deviation was 1.7%-4.1%, and the detection limit (LODs) was 0.03-0.05 mg/L. The method was simple, efficient and accurate, and suitable for the determination of acrylates in environmental water samples.

scholarly journals A Study on the Determination of Azithromycin by High-Performance Liquid Chromatography

2021 ◽  
Vol 5 (4) ◽  
pp. 125-130
Author(s):  
Yanli Zhuo
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Recovery Rate ◽  
High Performance ◽  
Drug Content ◽  
External Standard Method ◽  
Relative Standard ◽  
Theoretical Plate Number ◽  
Good Repeatability

Objective: To analyze the effect of high-performance liquid chromatography (HPLC) for the determination of azithromycin and to provide references for related research work. Methods: The mobile phase was ammonium dihydrogen phosphate at 0.067 mol/L (mixed with triethylamine; pH value was adjusted to 6.5). The chromatographic column was Kromasil C18 (250 mm × 4.6 mm; 5.0 ?m) and the relative standard deviation (RSD) of the drug content level was 1.25%. The injection volume was set to 20 ?L, the detection wavelength was set to 210 nm, the external standard method was used to complete the quantitative work, and the theoretical plate number should be more than 1000 according to the drug peak calculation. The effect of HPLC on the determination of azithromycin was analyzed. Results: The concentration of azithromycin was 1.40-3.40 mg/mL, and the linear relationship was good. RSD of the drug content level was 1.25%. The representative test product had strong stability within 8.0 hours and the method had good repeatability. According to the recovery experiment method, the recovery rates of three standard samples from low to high were 99.87%, 100.15%, and 100.62%. The average recovery rate was 100.21%. RSD value was 0.39%. It means that the recovery rate of HPLC is good. Conclusion: In the determination of azithromycin, the use of HPLC to complete the work was of high sensitivity, simple, and fast. The method had good repeatability in the determination of drug components which is worthy of further promotion.

Sign in / Sign up

Export Citation Format

Share Document