Fast quantification of the exhaled breath condensate of oxidative stress 8-iso-prostaglandin F2α using on-line solid-phase extraction coupled with liquid chromatography/electrospray ionization mass spectrometry

Talanta ◽  
2010 ◽  
Vol 82 (4) ◽  
pp. 1434-1438 ◽  
Author(s):  
Chien-Jen Wang ◽  
Ning-Hsiang Yang ◽  
Saou-Hsing Liou ◽  
Hui-Ling Lee
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Solid Phase ◽  
Exhaled Breath Condensate ◽  
Prostaglandin F2α ◽  
Ionization Mass ◽  
Phase Extraction ◽  
Exhaled Breath ◽  
On Line ◽  
Breath Condensate

scholarly journals NanoTiO2 Sunscreen Does Not Prevent Systemic Oxidative Stress Caused by UV Radiation and a Minor Amount of NanoTiO2 is Absorbed in Humans

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 888 ◽  
Author(s):  
Daniela Pelclova ◽  
Tomas Navratil ◽  
Tereza Kacerova ◽  
Blanka Zamostna ◽  
Zdenka Fenclova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Uv Radiation ◽  
Inductively Coupled Plasma ◽  
Exhaled Breath Condensate ◽  
Prostaglandin F2α ◽  
Minor Amount ◽  
Exhaled Breath ◽  
Skin Absorption ◽  
Systemic Oxidative Stress

The present pilot study tested the efficiency of nanoTiO2 sunscreen to prevent the oxidative stress/inflammation caused by ultraviolet (UV) radiation using biomarkers in subjects’ blood, urine, and exhaled breath condensate (EBC). In addition, the skin absorption of nanoTiO2 was studied. Six identical subjects participated in three tests: (A) nanoTiO2 sunscreen, (B) UV radiation, and (C) sunscreen + UV. The first samples were collected before the test and the second after sunscreen application and/or UV exposure. On day 4, the third samples were collected, and the sunscreen was washed off, and the fourth samples were collected on day 11. The following biomarkers were measured: malondialdehyde, 4-hydroxy-trans-hexenal, 4-hydroxy-trans-nonenal, aldehydes C6-C12, 8-iso-Prostaglandin F2α, o-tyrosine, 3-chlorotyrosine, 3-nitrotyrosine, 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine, 5-hydroxymethyl uracil, and leukotrienes, using liquid chromatography-electrospray ionisation-tandem mass spectrometry. Titania was measured using inductively coupled plasma mass spectrometry and TiO2 nanoparticles by transmission and scanning electron microscopy. Sunscreen alone did not elevate the markers, but UV increased the biomarkers in the plasma, urine, and EBC. The sunscreen prevented skin redness, however it did not inhibit the elevation of oxidative stress/inflammatory markers. Titania and nanoTiO2 particles were found in the plasma and urine (but not in the EBC) in all sunscreen users, suggesting their skin absorption.

scholarly journals Clinical-Scale High-Throughput Analysis of Urinary 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry with On-Line Solid-Phase Extraction

2006 ◽  
Vol 52 (7) ◽  
pp. 1381-1388 ◽  
Author(s):  
Chiung-Wen Hu ◽  
Chien-Jen Wang ◽  
Louis W Chang ◽  
Mu-Rong Chao
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Isotope Dilution ◽  
Solid Phase ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
On Line

Abstract Background: Quantification of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) in urine or blood is used to assess and monitor oxidative stress in patients. We describe the use of on-line solid-phase extraction (SPE) and isotope-dilution liquid chromatography–tandem mass spectrometry (LC-MS/MS) for automated measurement of urinary 8-oxodGuo. Methods: Automated purification of urine was accomplished with a switching valve and an Inertsil ODS-3 column. After the addition of 15N5-labeled 8-oxodGuo as an internal standard, urine samples were analyzed within 10 min without sample purification. This method was applied to measure urinary 8-oxodGuo in a group of healthy persons (32 regular smokers and 35 nonsmokers). Urinary cotinine was also assayed by an isotope-dilution LC-MS/MS method. Results: The lower limit of detection was 5.7 ng/L on column (2.0 fmol). Inter- and intraday imprecision (CV) was <5.0%. Mean recovery of 8-oxodGuo in urine was 99%–102%. Mean (SD) urinary concentrations of 8-oxodGuo in smokers [7.26 (3.14) μg/g creatinine] were significantly higher than those in nonsmokers [4.69 (1.70) μg/g creatinine; P <0.005]. Urinary concentrations of 8-oxodGuo were significantly correlated with concentrations of cotinine in smokers (P <0.05). Conclusions: This on-line SPE LC-MS/MS method is sufficiently sensitive, precise, and rapid to provide high-throughput direct analysis of urinary 8-oxodGuo without compromising quality and validation criteria. This method could be applicable for use in daily clinical practice for assessing oxidative stress in patients.

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