Comparative Histological and Immunohistochemical Study on the Effect of Platelet Rich Plasma / Minoxidil, Alone or in Combination, on Hair Growth in a Rat Model of Androgenic Alopecia

2022 ◽  
pp. 101726
Author(s):  
Sara Abdel Gawad Elsebay ◽  
Hoda Fouad Nada ◽  
Nancy Sultan Saad Sultan ◽  
Dalia Alaa El-Din Aly El-Waseef
Keyword(s):  
Rat Model ◽  
Hair Growth ◽  
Immunohistochemical Study ◽  
Platelet Rich Plasma ◽  
Androgenic Alopecia

The Safety and Efficacy of Mupirocin Topical Spray for Burn Wound Healing in A Rat Model

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Sritharadol Rutthapol ◽  
Chunhachaichana Charisopon ◽  
Kumlungmak Sukanjana ◽  
Buatong Wilaiporn ◽  
Dechraksa Janwit ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Immunohistochemical Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Burn Wound ◽  
Safety And Efficacy ◽  
Burn Wound Healing ◽  
Tgf Β1

ABSTRACT This study evaluated the effect of mupirocin topical spray on burn wound healing in a rat model. Fifteen male Sprague Dawley rats were used to create full-thickness burns on the rat dorsum using a cylindrical stainless steel rod. The rats were topically treated with normal saline solution (NSS), mupirocin spray, ointment, and solution. The wound size and morphological evaluation were investigated by photographs and clinical criterions for wound healing. The histology was observed by hematoxylin and eosin (HandE) staining assay. The immunohistochemical study was evaluated by detection of transforming growth factor-beta 1 (TGF-β1), and the ratio of matrix metalloproteinase-9 to the tissue inhibitor of matrix metalloproteinase-1 (MMP-9/TIMP-1) was quantified using the enzyme-linked immunosorbent assay (ELISA) assay. A complete healing was observed at 28 days in all treatments. Mupirocin formulations accelerated the wound healing faster than NSS in size. However, the clinical criteria indicated a desirable skin appearance in the mupirocin spray and ointment treated groups. The histological evaluations showed no differences between the treatments while the immunohistochemical study revealed that all treatments reduced the level of TGF-β1 over time, particularly on day 28 in the mupirocin spray and ointment treated groups. The MMP-9/TIMP-1 ratio was significantly lower in the mupirocin spray and ointment treated groups than in the NSS and mupirocin solution groups. This study shows the safety and efficacy in the use of mupirocin topical spray. The topical mupirocin spray is an alternative suitable for development as a human topical anti-infective and wound protection spray.

Platelet Rich Plasma Enhances Mechanical Strength of Strattice in Rat Model of Ventral Hernia Repair.

2021 ◽  
Author(s):  
Joseph S. Fernandez‐Moure ◽  
Jeffrey L. Van Eps ◽  
Jacob C. Scherba ◽  
Iman K. Yazdi ◽  
Andrew Robbins ◽  
...  
Keyword(s):  
Hernia Repair ◽  
Mechanical Strength ◽  
Ventral Hernia ◽  
Rat Model ◽  
Ventral Hernia Repair ◽  
Platelet Rich Plasma

scholarly journals Intracavernous Injection of Autologous Platelet-Rich Plasma Ameliorates Hyperlipidemia-Associated Erectile Dysfunction in a Rat Model

2021 ◽  
Vol 9 (2) ◽  
pp. 100317
Author(s):  
Yun-Ching Huang ◽  
Chun-Te Wu ◽  
Miao-Fen Chen ◽  
Yi-Hung Kuo ◽  
Jhy-Ming Li ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Intracavernous Injection ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

scholarly journals 277 Platelet-rich plasma for treating female androgenic alopecia: A systematic review

2021 ◽  
Vol 141 (5) ◽  
pp. S49
Author(s):  
G.E. Beraja ◽  
R.L. Quinonez ◽  
J.J. Jimenez
Keyword(s):  
Systematic Review ◽  
Platelet Rich Plasma ◽  
Androgenic Alopecia

Comparison of efficacy of topical 5% minoxidil alone versus topical 5% minoxidil with autologous Platelet Rich Plasma (PRP) therapy in treatment of androgenetic alopecia: A randomised open label study

2021 ◽  
Vol 7 (2) ◽  
pp. 107-114
Author(s):  
Bela Padhiar ◽  
Sruthy Raveendran
Keyword(s):  
Platelet Rich Plasma ◽  
Androgenetic Alopecia ◽  
Androgenic Alopecia ◽  
Open Label ◽  
Hair Density ◽  
Convenience Sample ◽  
Double Spin ◽  
Autologous Platelet Rich Plasma ◽  
Group B ◽  
Autologous Platelet

Androgenic alopecia (AGA) is a type of progressive hairloss, where there is androgen mediated conversion of susceptible terminal hairs into vellus hairs, in genetically predisposed individuals. To compare efficacy of Topical 5% Minoxidil alone versus Topical 5% Minoxidil with Autologous Platelet Rich Plasma (PRP) therapy in patients with Androgenetic Alopecia.This is aProspective study conducted in Department of Dermatology GMERS Medical College, Gandhinagar, Gujarat. A convenience sample of 62 men in the age group of 20-40 with Grade 2-5 AGA according to Hamilton- Norwood Grading were selected and was divided into 2 groups of 31 each. Presitting digital photographs and dermoscopic photos were taken. Autologous PRP was prepared using 18 ml of patients blood after double spin centrifugation and injected by Nappage technique. Results were assessed at the baseline and at the end of each sitting on the basis of change in hair density, photographic evaluation and patient’s self satisfaction. Highly significant increase in hair density was achieved after 4 months of treatment. At T4 (Fourth Session of treatment) Group B showed higher hair density (42.97± 8.96) as compared to Group A (36.94 ± 11.57) which was statistically significant at P = 0.03Group B showed better improvement as compared to Group A.PRP treatment has a positive therapeutic effect on male Androgenetic alopecia without major side effects.

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