The Safety and Efficacy of Mupirocin Topical Spray for Burn Wound Healing in A Rat Model

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Sritharadol Rutthapol ◽  
Chunhachaichana Charisopon ◽  
Kumlungmak Sukanjana ◽  
Buatong Wilaiporn ◽  
Dechraksa Janwit ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Immunohistochemical Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Burn Wound ◽  
Safety And Efficacy ◽  
Burn Wound Healing ◽  
Tgf Β1

ABSTRACT This study evaluated the effect of mupirocin topical spray on burn wound healing in a rat model. Fifteen male Sprague Dawley rats were used to create full-thickness burns on the rat dorsum using a cylindrical stainless steel rod. The rats were topically treated with normal saline solution (NSS), mupirocin spray, ointment, and solution. The wound size and morphological evaluation were investigated by photographs and clinical criterions for wound healing. The histology was observed by hematoxylin and eosin (HandE) staining assay. The immunohistochemical study was evaluated by detection of transforming growth factor-beta 1 (TGF-β1), and the ratio of matrix metalloproteinase-9 to the tissue inhibitor of matrix metalloproteinase-1 (MMP-9/TIMP-1) was quantified using the enzyme-linked immunosorbent assay (ELISA) assay. A complete healing was observed at 28 days in all treatments. Mupirocin formulations accelerated the wound healing faster than NSS in size. However, the clinical criteria indicated a desirable skin appearance in the mupirocin spray and ointment treated groups. The histological evaluations showed no differences between the treatments while the immunohistochemical study revealed that all treatments reduced the level of TGF-β1 over time, particularly on day 28 in the mupirocin spray and ointment treated groups. The MMP-9/TIMP-1 ratio was significantly lower in the mupirocin spray and ointment treated groups than in the NSS and mupirocin solution groups. This study shows the safety and efficacy in the use of mupirocin topical spray. The topical mupirocin spray is an alternative suitable for development as a human topical anti-infective and wound protection spray.

scholarly journals Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 287-292 ◽  
Author(s):  
AS Durmus ◽  
M. Yaman ◽  
HN Can
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Albino Rats ◽  
Burn Wound ◽  
Tissue Samples ◽  
Burned Areas ◽  
Positive Effects ◽  
Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P &lt; 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P &lt; 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model. &nbsp;

scholarly journals Tigecycline accelerates staphylococcal-infected burn wound healing through matrix metalloproteinase-9 modulation

2011 ◽  
Vol 67 (1) ◽  
pp. 191-201 ◽  
Author(s):  
O. Simonetti ◽  
O. Cirioni ◽  
G. Lucarini ◽  
F. Orlando ◽  
R. Ghiselli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Metalloproteinase 9

scholarly journals Effects of montelukast on burn wound healing in a rat model

2010 ◽  
Vol 33 (6) ◽  
pp. 413 ◽  
Author(s):  
Muhammet G Turtay ◽  
Cemal Firat ◽  
Emine Samdanci ◽  
Hakan Oguzturk ◽  
Serkan Erbatur ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Macrophage Infiltration ◽  
Control Group ◽  
Fibroblast Proliferation ◽  
Burn Wound ◽  
Leukotriene D4 ◽  
Topical Antibiotics ◽  
Burn Wound Healing ◽  
Local Burn

Purpose: Montelukast, a selective cysteinyl leukotriene D4-receptor antagonist, is used in the treatment of asthma. In a rat model, our aim was to investigate the effects of montelukast, alone or in combination with topical antibiotics, on local burn wound healing. Methods: Rats were randomly allocated to four groups after local burn development: Group 1; rats were left to secondary healing without treatment, Group 2; a dose of 10 mg/kg montelukast was given by gastric gavage once a day for 10 days, Group 3; rats were treated with topical pomade (bacitracin neomycin sulphate), and Group 4; rats were treat with a combination of topical antibiotic and montelukast (10 mg/kg were given by gastric gavage once a day for 10 days). Skin biopsies were taken on days 3, 10, 14, and 20 relative to burn induction. Results: Reepithelialization in the pomade and montelukast+pomade groups on the 10th day was significantly greater, in comparison with control and montelukast groups (p < 0.05). For the montelukast group, edema (on the 14th day) and angiogenesis, fibroblast proliferation, edema and macrophage infiltration (on the 20th day) were statistically improved in comparison with the control group (p < 0.05). For the montelukast+pomade group, angiogenesis, fibroblast proliferation and macrophage infiltration (on the 10th day), and angiogenesis, fibroblast proliferation, edema and macrophage infiltration (on the 14th and 20th days) were statistically improved in comparison with the control group (p < 0.05). Conclusion: In conclusion, montelukast was effective on burn wound healing. Moreover, the effect was amplified when combined with topical antibiotics applied in the early stage of burn wound healing.

Burn Wound Healing in the K14/TGF-β1 Transgenic Mice

2001 ◽  
Vol 22 ◽  
pp. S49
Author(s):  
E. E. Tredget ◽  
L. Yang ◽  
T. Chan ◽  
J. T. Demare ◽  
T. Iwashina ◽  
...  
Keyword(s):  
Wound Healing ◽  
Transgenic Mice ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Tgf Β1

scholarly journals Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

2016 ◽  
Vol 39 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Guangbing Wei ◽  
Xin Chen ◽  
Guanghui Wang ◽  
Lin Fan ◽  
Kang Wang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Adhesion Formation ◽  
Adhesion Prevention ◽  
Enzyme Linked Immunosorbent Assay ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Tnf Α ◽  
Tgf Β1

Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

Evaluation of ROCEN on Burn Wound Healing and Thermal Pain: Transforming Growth Factor-β1 Activation

2020 ◽  
pp. 153473462091532
Author(s):  
Ramin Goudarzi ◽  
Maryam Eskandary Nasab ◽  
Partow Mirzaee Saffari ◽  
Golnaz Zamanian ◽  
Chong Deok Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Burn Injuries ◽  
Tail Flick ◽  
Burn Wound ◽  
Thermal Pain ◽  
Burn Wound Healing ◽  
Short Period

The present study aimed to evaluate the effect of liposomal arthrocen 2% (ROCEN) on the healing of burn wound and pain alleviation of thermal stimuli in a rat model of the second-degree burn. The results showed that ROCEN formulation significantly improved the main parameters of burn wound healing in a short period of time (7 days). The percentage of wound surface was also reduced significantly compared with the control group following once daily application of ROCEN for 14 days. The level of TGF (transforming growth factor)-β1 cytokine was also elevated significantly in the burn tissue treated with ROCEN almost the same as zinc oxide cream. Also, ROCEN showed a significant analgesic effect evaluated by 2 models of acute thermal pain, tail-flick and hotplate tests, which suggested that the formulation may act as a pain reliever in burn injuries. In conclusion, the application of the topical formulation of ROCEN may have benefits in the acceleration of the wound healing process and alleviation of the pain due to burn injuries.

scholarly journals Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Imran Khan ◽  
Saeed Ur Rahman ◽  
Elieza Tang ◽  
Karl Engel ◽  
Bradford Hall ◽  
...  
Keyword(s):  
Wound Healing ◽  
Surface Temperature ◽  
Molecular Mechanisms ◽  
Tissue Injury ◽  
Cell Types ◽  
Wound Management ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Tissue Healing ◽  
Tgf Β1

AbstractThe severity of tissue injury in burn wounds from associated inflammatory and immune sequelae presents a significant clinical management challenge. Among various biophysical wound management approaches, low dose biophotonics treatments, termed Photobiomodulation (PBM) therapy, has gained recent attention. One of the PBM molecular mechanisms of PBM treatments involves photoactivation of latent TGF-β1 that is capable of promoting tissue healing and regeneration. This work examined the efficacy of PBM treatments in a full-thickness burn wound healing in C57BL/6 mice. We first optimized the PBM protocol by monitoring tissue surface temperature and histology. We noted this dynamic irradiance surface temperature-monitored PBM protocol improved burn wound healing in mice with elevated TGF-β signaling (phospho-Smad2) and reduced inflammation-associated gene expression. Next, we investigated the roles of individual cell types involved in burn wound healing following PBM treatments and noted discrete effects on epithelieum, fibroblasts, and macrophage functions. These responses appear to be mediated via both TGF-β dependent and independent signaling pathways. Finally, to investigate specific contributions of TGF-β1 signaling in these PBM-burn wound healing, we utilized a chimeric TGF-β1/β3 knock-in (TGF-β1Lβ3/Lβ3) mice. PBM treatments failed to activate the chimeric TGF-β1Lβ3/Lβ3 complex and failed to improve burn wound healing in these mice. These results suggest activation of endogenous latent TGF-β1 following PBM treatments plays a key role in burn wound healing. These mechanistic insights can improve the safety and efficacy of clinical translation of PBM treatments for tissue healing and regeneration.

scholarly journals Adipose-derived stem cells improve grafted burn wound healing by promoting wound bed blood flow

2020 ◽  
Vol 8 ◽  
Author(s):  
Osamu Fujiwara ◽  
Anesh Prasai ◽  
Dannelys Perez-Bello ◽  
Amina El Ayadi ◽  
Irene Y Petrov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Blood Flow ◽  
Topical Application ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adipose Derived Stem Cells ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Cell Based Therapy ◽  
Donor Skin

Abstract Background Researchers have explored the use of adipose-derived stem cells (ASCs) as a cell-based therapy to cover wounds in burn patients; however, underlying mechanistic aspects are not completely understood. We hypothesized that ASCs would improve post-burn wound healing after eschar excision and grafting by increasing wound blood flow via induction of angiogenesis-related pathways. Methods To test the hypothesis, we used an ovine burn model. A 5 cm2 full thickness burn wound was induced on each side of the dorsum. After 24 hours, the burned skin was excised and a 2 cm2 patch of autologous donor skin was grafted. The wound sites were randomly allocated to either topical application of 7 million allogeneic ASCs or placebo treatment (phosphate-buffered saline [PBS]). Effects of ASCs culture media was also compared to those of PBS. Wound healing was assessed at one and two weeks following the application of ASCs. Allogeneic ASCs were isolated, cultured and characterized from non-injured healthy sheep. The identity of the ASCs was confirmed by flow cytometry analysis, differentiation into multiple lineages and gene expression via real-time polymerase chain reaction. Wound blood flow, epithelialization, graft size and take and the expression of vascular endothelial growth factor (VEGF) were determined via enzyme-linked immunosorbent assay and Western blot. Results Treatment with ASCs accelerated the patch graft growth compared to the control (p &lt; 0.05). Topical application of ASCs significantly increased wound blood flow (p &lt; 0.05). Expression of VEGF was significantly higher in the wounds treated with ASCs compared to control (p &lt; 0.05). Conclusions ASCs accelerated grafted skin growth possibly by increasing the blood flow via angiogenesis induced by a VEGF-dependent pathway.

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