Introduction:
A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC)
method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances
Materials and Methods:
Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction
with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow
at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno,
Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute,
4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of
2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were
between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to
101.23±1.39% for Paracetamol.
Results and Discussion:
The validated optimal protocol is robust and accurate for simultaneous analysis
of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as
pharmaceutical formulation.
Conclusion:
In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection
was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical
preparations.
Immobilization of Pseudomonas aeruginosa static biomass on eggshell powder for on-line preconcentration and determination of Cr (VI)