Lactobacillus paracasei alleviates genotoxicity, oxidative stress status and histopathological damage induced by Fumonisin B1 in BALB/c mice

Abstract Fumonisin B1 (FB1) is a carcinogenic (Class 2B) mycotoxin produced by Fusarium fungi and is responsible for several types of mycotoxicoses in animals and humans. Lactobacillus paracasei (LP), as a probiotic, is known to impart a wide range of advantageous effects on host health. The objective of the current study was to evaluate if LP (type BEJ01) isolated from Tunisian artisanal butter, could potentially help protect a host against intestinal alterations caused by exposure to FB1. Here, adult male Balb/c mice were randomly assigned to four groups, i.e., control (vehicle only), treated with FB1 (100 µg/kg BW), LP (2 × 109 CFU/ml [≈ 2 mg/kg BW]) and FB1 (100 µg/kg BW) + LP (2 × 109 CFU/ml) and treated per os daily for 10 days. At 24 hr after the final treatment, mice were euthanized and their jejunum harvested for examination of intestinal alterations induced by the FB1. The data showed that a variety of negative effects in jejunal tissue were induced by the FB1, including DNA fragmentation, oxidative stress, apoptotic cell death, and histopathological alterations. The results also showed that co-treatment of LP with FB1 was able to mitigate the harmful FB1 effects. LP alone imparted no damage to jejunal tissues. These results show that apart from potential use as a bio-preservative for extending the shelf-life of food and feeds, LP may also provide a benefit of helping prevent untoward effects from a potent mycotoxin like FB1.

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The Ameliorative Effect of Lactobacillus Paracasei BEJ01 Against FB1 Induced Spermatogenesis Disturbance, Testicular Oxidative Stress and Histopathological Damage

10.21203/rs.3.rs-1162613/v1 ◽

2021 ◽

Author(s):

Khawla EZDINI ◽

Jalila Ben salah-Abbès ◽

Hela Belgacem ◽

kamel chaieb ◽

Samir Abbès

Keyword(s):

Oxidative Stress ◽

Reproductive System ◽

Sperm Quality ◽

Harmful Effect ◽

Fumonisin B1 ◽

Lactobacillus Paracasei ◽

Stress Generation ◽

Economic Losses ◽

Control Group ◽

Wide Range

Abstract Fumonisin B1 (FB1) was a possible carcinogenic molecule for humans as classified by the IARC on 2B group. In livestock, it was responsible for several mycotoxicosis and economic losses. Lactobacillus strains, inhabitants of a wide range of foodstuffs as well as our gastro-intestinal tract, were Generally Recognized as Safe (GRAS). Thus, the aim of this work was to evaluate the protective effect of Lactobacillus paracasei (LP) against FB1 induced reprotoxicities including testicular histopathology, sperm quality disturbance and testosterone level reduction. Pubescent mice were divided randomly into four groups as bellow: Group1: Control; Group 2: FB1 (100 µg/kg b.w); Group 3: LP (2× 109 CFU/kg b.w); Group 4: LP (2× 109 CFU/kg b.w) and FB1 (100 µg/kg b.w) to be then treated for 10 days. After the end of the treatment, animals were sacrificed; the plasma and epididyms and testicles were harvested to the reproductive system studies. Our results highlighted that LP counteracted the harmful effect generated by FB1. Indeed; it induced sperm quality reduction, oxidative stress generation and histological alterations. In conclusion, the used strain was able to prevent FB1-reproductive system damages of in balb/c mice and could be valorised as an anti-cating agent in animal FB1-contaminated diet.

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Oxidative Stress Status in Patients with Choledocholithiasis: Before and After Endoscopic Sphincterotomy and Biliary Clearance

Revista de Chimie ◽

10.37358/rc.18.8.6493 ◽

2018 ◽

Vol 69 (8) ◽

pp. 2172-2176

Author(s):

Catalin Victor Sfarti ◽

Alin Ciobica ◽

Carol Stanciu ◽

Gheorghe G. Balan ◽

Irina Garleanu ◽

...

Keyword(s):

Oxidative Stress ◽

Endoscopic Sphincterotomy ◽

Specific Activity ◽

Oxidative Stress Marker ◽

Control Group ◽

Oxidative Stress Parameter ◽

Extrahepatic Cholestasis ◽

Biliary Clearance ◽

Oxidative Stress Status ◽

Before And After

Choledocholithiasis may cause biliary obstruction which leads to hepatocellular injury. Oxidative stress has been proposed as a possible mechanism involved in this disorder. This study evaluates the oxidative stress burden in patients with choledocholithiasis and secondary cholestasis, before and after endoscopic sphincterotomy. Experimental part: Patients diagnosed with choledocholithiasis and secondary extrahepatic cholestasis were included in the study between January 1st 2016 and October 31st 2016. In all patients oxidative stress markers were collected within 2 hours before and 48 hours after therapeutic ERCP. Selected markers were superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA). The results were compared to those from a group of 40 healthy subjects. Significantly lower concentrations of SOD (p = 0.03) and GPX (p [ 0.0001) activities, associated with an increased level of MDA level (p [ 0.0001) were shown in patients before biliary clearance compared with the healthy control group. After ERCP the only oxidative stress parameter which showed improvement was the SOD specific activity (p = 0.037). This study shows that extrahepatic cholestasis secondary to choledocholithiasis is associated with increased oxidative stress status. After biliary clearance one oxidative stress marker was significantly improved (SOD), suggesting a possible antioxidant effect of such procedure.

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Modulatory Role of Riboceine and Vitamin C Supplementation on Altered Oxidative Stress Status of Alloxan-induced Diabetic Wistar Rats

Bioscience Biotechnology Research Communications ◽

10.21786/bbrc/13.4/75 ◽

2020 ◽

Vol 13 (4) ◽

pp. 2130-2138

Author(s):

Akingbade Bamidele Thomas

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Wistar Rats ◽

Oxidative Stress Status ◽

Vitamin C Supplementation

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Oxidative stress status: An introduction

Free Radical Biology and Medicine ◽

10.1016/0891-5849(91)90071-a ◽

1991 ◽

Vol 10 (3-4) ◽

pp. 173 ◽

Cited By ~ 13

Author(s):

William A. Pryor ◽

Susan Shipley Godber

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Status

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Curcumin Potentiates α7 Nicotinic Acetylcholine Receptors and Alleviates Autistic-Like Social Deficits and Brain Oxidative Stress Status in Mice

International Journal of Molecular Sciences ◽

10.3390/ijms22147251 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7251

Author(s):

Petrilla Jayaprakash ◽

Dmytro Isaev ◽

Waheed Shabbir ◽

Dietrich E. Lorke ◽

Bassem Sadek ◽

...

Keyword(s):

Oxidative Stress ◽

Nicotinic Acetylcholine Receptors ◽

Acetylcholine Receptors ◽

Pathological Condition ◽

Repetitive Behaviors ◽

Social Deficits ◽

Nicotinic Acetylcholine ◽

Oxidative Stress Status ◽

Inward Currents ◽

Α7 Nachrs

Autistic spectrum disorder (ASD) refers to a group of neurodevelopmental disorders characterized by impaired social interaction and cognitive deficit, restricted repetitive behaviors, altered immune responses, and imbalanced oxidative stress status. In recent years, there has been a growing interest in studying the role of nicotinic acetylcholine receptors (nAChRs), specifically α7-nAChRs, in the CNS. Influence of agonists for α7-nAChRs on the cognitive behavior, learning, and memory formation has been demonstrated in neuro-pathological condition such as ASD and attention-deficit hyperactivity disorder (ADHD). Curcumin (CUR), the active compound of the spice turmeric, has been shown to act as a positive allosteric modulator of α7-nAChRs. Here we hypothesize that CUR, acting through α7-nAChRs, influences the neuropathology of ASD. In patch clamp studies, fast inward currents activated by choline, a selective agonist of α7-nAChRs, were significantly potentiated by CUR. Moreover, choline induced enhancement of spontaneous inhibitory postsynaptic currents was markedly increased in the presence of CUR. Furthermore, CUR (25, 50, and 100 mg/kg, i.p.) ameliorated dose-dependent social deficits without affecting locomotor activity or anxiety-like behaviors of tested male Black and Tan BRachyury (BTBR) mice. In addition, CUR (50 and 100 mg/kg, i.p.) mitigated oxidative stress status by restoring the decreased levels of superoxide dismutase (SOD) and catalase (CAT) in the hippocampus and the cerebellum of treated mice. Collectively, the observed results indicate that CUR potentiates α7-nAChRs in native central nervous system neurons, mitigates disturbed oxidative stress, and alleviates ASD-like features in BTBR mice used as an idiopathic rodent model of ASD, and may represent a promising novel pharmacological strategy for ASD treatment.

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Positive Modulation of PinkNelumbo nuciferaFlowers on Memory Impairment, Brain Damage, and Biochemical Profiles in Restraint Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/5789857 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

Thawatchai Prabsattroo ◽

Jintanaporn Wattanathorn ◽

Pichet Somsapt ◽

Opass Sritragool

Keyword(s):

Oxidative Stress ◽

Monoamine Oxidase ◽

Spatial Memory ◽

Brain Damage ◽

Memory Deficit ◽

Ki67 Expression ◽

Mao B ◽

Serum Corticosterone ◽

Neuron Density ◽

Oxidative Stress Status

Due to the crucial role of oxidative stress in the stress-induced memory deficit, the benefit of substance possessing antioxidant effect is focused. Since no data are available, we aimed to determine the effect ofNelumbo nuciferaflowers extract on spatial memory and hippocampal damage in stressed rats. Male Wistar rats, weighing 250–350 g, were orally givenN. nuciferaextract at doses of 10, 10, and 200 mg·kg−145 minutes before the exposure to 12-hour restraint stress. The spatial memory and serum corticosterone were assessed at 7 and 14 days of study period. At the end of study, acetylcholinesterase (AChE), monoamine oxidase type A and monoamine oxidase type B (MAO-A and MAO-B), oxidative stress status, neuron density, and Ki67 expression in hippocampus were also assessed. The results showed thatN. nuciferaextract decreased memory deficit and brain damage, serum corticosterone, oxidative stress status, AChE, and MAO-A and MAO-B activities but increased neuron density and Ki67 expression in hippocampus. These suggested that the improved oxidative stress status, adult neurogenesis, and cholinergic and monoaminergic functions might be responsible for the protective effect against stress-related brain damage and dysfunction of the extract. Therefore,N. nuciferaextract is the potential neuroprotective and memory enhancing agent. However, further researches are still required.

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Autistic-Like Behaviors, Oxidative Stress Status, and Histopathological Changes in Cerebellum of Valproic Acid Rat Model of Autism Are Improved by the Combined Extract of Purple Rice and Silkworm Pupae

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/3206561 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 13

Author(s):

Nartnutda Morakotsriwan ◽

Jintanaporn Wattanathorn ◽

Woranan Kirisattayakul ◽

Kowit Chaisiwamongkol

Keyword(s):

Oxidative Stress ◽

Valproic Acid ◽

Purkinje Cell ◽

Rat Model ◽

Cell Loss ◽

Hot Plate ◽

Hot Plate Test ◽

Silkworm Pupae ◽

Oxidative Stress Status ◽

Purkinje Cell Loss

Due to the crucial role of oxidative stress on the pathophysiology of autism and the concept of synergistic effect, the benefit of the combined extract of purple rice and silkworm pupae (AP1) for autism disorder was the focus. Therefore, we aimed to determine the effect of AP1 on autistic-like behaviors, oxidative stress status, and histopathological change of cerebellum in valproic acid (VPA) rat model of autism. VPA was injected on postnatal day (PND) 14 and the animals were orally given AP1 at doses of 50, 100, and 200 mg·kg−1BW between PND 14 and PND 40. The autism-like behaviors were analyzed via hot-plate, rotarod, elevated plus-maze, learning, memory, and social behavior tests. Oxidative stress and the histological change in the cerebellum were assessed at the end of study. AP1 treated rats improved behaviors in all tests except that in hot-plate test. The improvement of oxidative stress and Purkinje cell loss was also observed in the cerebellum of VPA-treated rats. Our data suggest that AP1 partially reduced autism-like behaviors by improving oxidative stress and Purkinje cell loss. Further research is required to identify the active ingredients in AP1 and gender difference effect.

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