Effectiveness of ozonated water on Candida albicans, Enterococcus faecalis, and endotoxins in root canals

2008 ◽  
Vol 105 (3) ◽  
pp. e85-e91 ◽  
Author(s):  
Marcelo Gonçalves Cardoso ◽  
Luciane Dias de Oliveira ◽  
Cristiane Yumi Koga-Ito ◽  
Antonio Olavo Cardoso Jorge
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canals ◽  
Ozonated Water

scholarly journals Evaluation of Synergetic Antibacterial Effect of Gaseous Ozone and Ozonated Water against Enterococcus Faecalis in Human Root Canals

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Can Banu Uygun ◽  
Özbay Gizem ◽  
Altundal Hatice ◽  
Durmazpinar Parla Meva ◽  
Kitiki Burak ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Root Canals ◽  
Gaseous Ozone ◽  
Ozonated Water

scholarly journals Antimicrobial activity of sodium hypochlorite associated with intracanal medication for Candida albicans and Enterococcus faecalis inoculated in root canals

2009 ◽  
Vol 17 (6) ◽  
pp. 555-559 ◽  
Author(s):  
Marcia Carneiro Valera ◽  
Katy Costa Godinho da Silva ◽  
Lilian Eiko Maekawa ◽  
Cláudio Antonio Talge Carvalho ◽  
Cristiane Yumi Koga-Ito ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canals

scholarly journals In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals

2013 ◽  
Vol 21 (2) ◽  
pp. 118-123 ◽  
Author(s):  
Marcia Carneiro Valera ◽  
Lilian Eiko Maekawa ◽  
Luciane Dias de Oliveira ◽  
Antonio Olavo Cardoso Jorge ◽  
Erika Shygei ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Chemical Substances ◽  
In Vitro Antimicrobial Activity ◽  
Root Canals ◽  
Natural Extracts

scholarly journals Synergistic Antimicrobial Action of Chlorhexidine and Ozone in Endodontic Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Rita Noites ◽  
Cidália Pina-Vaz ◽  
Rita Rocha ◽  
Manuel Fontes Carvalho ◽  
Acácio Gonçalves ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Endodontic Treatment ◽  
Periapical Lesions ◽  
Root Canals ◽  
Single Root ◽  
Gaseous Ozone ◽  
Infected Root

Objectives. The aim of this study was to determine whether irrigation with sodium hypochlorite, chlorhexidine, and ozone gas, alone or in combination, were effective againstEnterococcus faecalisandCandida albicans; these are microorganisms frequently isolated from teeth with periapical lesions resistant to endodontic treatment.Material and Methods. 220 single root teeth, recently extracted, were inoculated withCandida albicansandEnterococcus faecalis. The formulations tested were sodium hypochlorite at 1, 3, and 5% chlorhexidine at 0.2% and 2% and ozone gas applied for different periods of time. The combination of sodium hypochlorite at 5% and chlorhexidine at 2%, with gaseous ozone, were also assessed. For the most active treatments the mechanism of action was assessed through flow cytometry.Results. Sodium hypochlorite, chlorhexidine, and gaseous ozone alone were ineffective in completely eliminating the microorganisms. The association of chlorhexidine at 2% followed by ozone gas for 24 seconds promoted the complete elimination ofCandida albicansandEnterococcus faecalis. Flow cytometry shows that ozone and chlorhexidine act differently, which could explain its synergic activity.Conclusions. This new disinfection protocol, combining irrigation with chlorhexidine at 2% and ozone gas for 24 seconds, may be advantageous when treating infected root canals.

scholarly journals Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

2016 ◽  
Vol 17 (2) ◽  
pp. 114-118 ◽  
Author(s):  
Marcia C Valera ◽  
Sarah AC Oliveira ◽  
Lilian E Maekawa ◽  
Flávia GR Cardoso ◽  
Adriana Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Zingiber Officinale ◽  
Human Teeth ◽  
Root Canals

ABSTRACT Aim The purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CHX) as auxiliary chemical substance and intracanal medications on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and their endotoxins in the root canals. Materials and methods The study was conducted on 48 single-rooted human teeth divided into four groups (n = 12), according to intracanal medications used: (1) Calcium hydroxide + apyrogenic saline solution (Ca(OH)2 + SS), (2) 20% ginger glycolic extract (GEN), (3) calcium hydroxide + 20% ginger glycolic extract (Ca(OH)2 + GEN), (4) apyrogenic SS (control). Collections were made from the root canal content before preparation (baseline–S1), immediately after instrumentation (S2), 7 days after instrumentation (S3), after 14 days the action of intracanal medication (S4), and 7 days after removal of the intracanal medication (S5). The antimicrobial activity and endotoxin content were analyzed for all collections. The results were statistically analyzed by the Kruskal–Wallis and Dunn tests at a significance level of 5%. Results After instrumentation with CHX, there was complete elimination of E. coli and C. albicans, except for E. faecalis, which was significantly reduced and then completely eliminated after intracanal medication. There was significant reduction of endotoxin after instrumentation. Comparison of collection after instrumentation and intracanal medication revealed reduction of endotoxins in all groups; this reduction was greater in group Ca(OH)2 followed by the group GEN. Conclusion It was concluded that the instrumentation using CHX and intracanal medication used were able to eliminate the microorganisms from the root canal; the endotoxins were reduced, yet not completely eliminated. Clinical significance This study is important and relevant for searching alternatives during endodontic therapy, since it aims to study the effect of Zingiber officinale on microorganisms and endotoxins present in root canals. How to cite this article Valera MC, Oliveira SAC, Maekawa LE, Cardoso FGR, Chung A, Silva SFP, Carvalho CAT. Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals. J Contemp Dent Pract 2016; 17(2):114-118.

scholarly journals Effect of photodynamic therapy using diode laser and a novel plant extract irrigant against Enterococcus faecalis and Candida albicans

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Doaa M. Sadony ◽  
Maram E. Khallaf ◽  
Jackline Samir Kamal
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Moringa Oleifera ◽  
Bacterial Count ◽  
Control Group ◽  
Root Canals ◽  
Laser Group ◽  
Group 10

Abstract Background To evaluate the antibacterial effect of diode laser and photodynamic therapy using methylene blue dye when used alone or in combination with Moringa oleifera extract on root canal microbes. A total of 50 single root canals with single straight canals were prepared with ProTaper files, sterilized in an autoclave device, contaminated with root canal microbes (Enterococcus faecalis and Candidia albicans) and placed in incubator for 21 days. They were then randomly divided into 6 groups. Laser group: (10 root canals) were subjected to laser only. Moringa group: (10 root canals) were irrigated using plant extract only (Moringa). PDT group: (10 root canals) were irrigated using Moringa and Methylene blue then subjected to laser. Methylene blue and laser group: (10 root canals) were irrigated using methylene blue then subjected to laser. Negative control group: (10 root canals) weren't irrigated. After treatment microbial assessment (colony count) was done. Data was analyzed using ANOVAs test. Results Moringa/laser group and PDT group showed statistically least bacterial count for both E. faecalis and Candida albicans. Moringa only group showed statistically less bacterial count than control group. Conclusion The combined use of PDT together with Moringa oleifera has the best antimicrobial effect.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

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