scholarly journals Promotion of the Hypocrellin Yield by a Co-Culture of Shiraia bambusicola (GDMCC 60438) with Arthrinium sp. AF-5 Fungus

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 316
Author(s):  
Xiaoxiao Yan ◽  
Yongdi Wen ◽  
Menghua Hu ◽  
Zhenqiang Wu ◽  
Xiaofei Tian
Keyword(s):  
Carbon Source ◽  
Submerged Fermentation ◽  
Microscope Observation ◽  
Time Increase ◽  
Anthracene Derivative ◽  
Shiraia Bambusicola ◽  
Hypocrellin A ◽  
Cultivation Strategy ◽  
Fermentation Media

Hypocrellin is a natural 3,10-xylene-4,9-anthracene derivative compound that originates from the stroma of Shiraia bambusicola (S. bambusicola) and Hypocrella bambusae with excellent photobiological activities. Submerged fermentation with the mycelia of S. bambusicola is generally regarded as an ideal technology for hypocrellin production. This study developed a co-cultivation strategy for an obvious promotion of the hypocrellin yield by incubating S. bambusicola (GDMCC 60438) with the endophyte fungus Arthrinium sp. AF-5 isolated from the bamboo tissue. The results indicated that the yield of hypocrellin A (HA) reached a 66.75 mg/g carbon source after an 84-h co-cultivation of the two strains, which was a four-time increase of that by the fermentation only with the S. bambusicola. The microscope observation found that the mycelia of the two strains were intertwined with each other to form the mycelium pellets during the co-cultivation. Moreover, the mycelium pellets of the co-culture showed a contracted and slightly damaged morphology. The addition of H2O2 in the fermentation media could further increase the HA production by 18.31%.

scholarly journals Studies on Nisin Production by Isolated Lactobacillus Species in Aqueous Two-phase System

2007 ◽  
Vol 6 (1) ◽  
pp. 69-80
Author(s):  
Namita Singh ◽  
S. K. Mandal
Keyword(s):  
Carbon Source ◽  
Submerged Fermentation ◽  
Biomass Yield ◽  
Cheese Whey ◽  
Maximum Yield ◽  
Lactobacillus Species ◽  
Phase System ◽  
Two Phase ◽  
Nisin Production ◽  
Aqueous Two Phase System

Isolated lactobacillus sp.(from cheese whey) was examined for its ability to produce bacteriocin like nisin in submerged fermentation and aqueous two-phase system(ATPS) at pH 6.5, 30.C for 18 h fermentation using M17S medium with 5%(v/v) inoculum and the nisin yields were obtained 4500 IU/ml and 20216 IU/ml where as biomass yield obtained 19.55 mg/ml and 31.88 mg/ml respectively.The maximum yield of nisin (24800 IU/ml) and biomass (22.49 mg/ml) were obtained in ATPS (28% PEG 6000 and 4% MgSO4.7H20 with 5-times concentrated M17 medium containing 0.5% lactose as carbon source) under similar conditions.

Gentic overexpression increases production of hypocrellin A in Shiraia bambusicola S4201

2019 ◽  
Vol 57 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Dan Li ◽  
Ning Zhao ◽  
Bing-Jing Guo ◽  
Xi Lin ◽  
Shuang-Lin Chen ◽  
...  
Keyword(s):  
Shiraia Bambusicola ◽  
Hypocrellin A

A HPLC-UV Method for Simultaneous Determination of Hypocrellin A and B from Fermentation Broth of Shiraia Bambusicola LPHP-89

2015 ◽  
Vol 1092-1093 ◽  
pp. 662-665
Author(s):  
Sai Dan Zhang ◽  
Ping Lv ◽  
Xiu Zhu
Keyword(s):  
High Performance ◽  
Fermentation Broth ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Shiraia Bambusicola ◽  
Rp Hplc ◽  
Hypocrellin A ◽  
Hypocrellin B ◽  
Liquid Chromatographic

A rapid, sensitive and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous quantification of hypocrellin A, hypocrellin B from Fermentation Broth of Shiraia bambusicola LPHP-89. Isocratic RP-HPLC system with C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) and a detector UV-VWD was employed. The mobile phase consisted of methanol、water and acetic acid (60:40:1, v/v/v) and was pumped at a flow rate of 1.0 mL/min. The injection volume was5 μL. The quantitation wavelength was set at 254 nm.Total run time was 20 min and retention times for hypocrellin A and hypocrellin B were 10.06 and 15.38 min, respectively.

Sign in / Sign up

Export Citation Format

Share Document