Molecular identification of Cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of São Paulo, Brazil

2007 ◽  
Vol 150 (4) ◽  
pp. 291-296 ◽  
Author(s):  
Alexandre Thomaz ◽  
Marcelo V. Meireles ◽  
Rodrigo M. Soares ◽  
Hilda F.J. Pena ◽  
Solange M. Gennari
2015 ◽  
Vol 67 (2) ◽  
pp. 441-446 ◽  
Author(s):  
A.S. Zucatto ◽  
M.C.C. Aquino ◽  
S.V. Inácio ◽  
R.N. Figueiredo ◽  
J.C. Pierucci ◽  
...  

Considering the proximity of sheep farmers to animals that are possibly diseased or releasing fecal oocysts into the environment and the marked pathogenicity in lambs, the aim of this study was to determine the occurrence and to molecularly characterize the infection by Cryptosporidium spp. in lambs in the South Central region of the state of São Paulo, Brazil. A total of 193 fecal samples were collected from sheep of several breeds, males and females, aged up to one year. Polymerase chain reaction (nested-PCR) was used to amplify DNA fragments from the subunit 18S rRNA gene and indicated 15% positivity; sequencing of amplified fragments was possible for 19 samples. Analysis of the obtained sequences showed that the identified species were Cryptosporidium xiaoi for 15 samples, constituting thus the first molecular characterization study of this Cryptosporidium species in Brazil. Cryptosporidium ubiquitum was identified for three samples and Cryptosporidium meleagridis for one sample; the latter two are considered zoonotic species.


2016 ◽  
Vol 37 (6) ◽  
pp. 4157 ◽  
Author(s):  
Denise Junqueira Matos ◽  
Marcelo Vasconcelos Meireles ◽  
Willian Marinho Dourado Coelho ◽  
Katia Denise Saraiva Bresciani

Cryptosporidium spp. and Giardia spp. are parasites and have been reported in many species of mammals, including humans. The goal of this research was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in 45-days-old pigs. Fecal samples of 107 pigs were collected at three alternate days in piggeries in Araçatuba, São Paulo State, Brazil. Cryptosporidium oocysts were observed in 4.7% (5/107) of animals by Kinyoun acid-fast stain method and cysts of Giardia spp. were observed in 1.9% (2/107) of the animals by the method of Faust. Of all animals, 85 presented feces with normal consistency and 22 showed diarrhea in at least one collection. Two diarrheic samples showed positivity for both Cryptosporidium spp. and Giardia spp. From these results it is possible to infer that the occurrence of Cryptosporidium and Giardia was low because of the good management practices and both protozoa were not associated the presence of symptoms.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4207 ◽  
Author(s):  
Huber Rizzo ◽  
Lilian Gregory ◽  
Fidel Beraldi ◽  
Aline Feola de Carvalho ◽  
Eliana Scarcelli Pinheiro

Campylobacter species are a significant cause of sheep abortion in most sheep-raising countries. The relationship between the presence of Campylobacter spp. in fecal samples and reproductive disorders was investigated in 274 sheep from 28 properties in the state of São Paulo, Brazil. Biological samples from 16 aborted fetuses, one uterus, six placentas, five uterine secretion samples, five vaginal swabs, 17 semen samples, and three preputial swabs were also subjected to bacterial isolation. The bacteria were isolated from fecal samples of 14.9% (5/28) of the properties, affecting 3.65% (10/274) of the sheep, 3.5% (9/255) of females and 5.3% (1/19) of males. Campylobacter jejuni was the most prevalent species, present in 66.67% (7) of the positive samples, followed by Campylobacter coli, present in 22.22% (2), and one strain was identified as Campylobacter spp. The birth of “weak” lambs (p=0.06, OR=6.83 and CI=1.73 to 27.05) and neonatal death (p=0.087, OR=3.5 and CI=0.83 to 14.72) were associated with the fecal isolation of Campylobacter spp. Diarrhea was also associated with the bacteria (p=0.003, OR=9.83 and CI=2.19 to 44.18). The dissemination of Campylobacter spp. in Brazilian sheep is low and that, at present, the existing strains are not responsible for significant economic losses in sheep production, especially in adult animals.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4207
Author(s):  
Huber Rizzo ◽  
Lilian Gregory ◽  
Fidel Beraldi ◽  
Aline Feola de Carvalho ◽  
Eliana Scarcelli Pinheiro

Campylobacter species are a significant cause of sheep abortion in most sheep-raising countries. The relationship between the presence of Campylobacter spp. in fecal samples and reproductive disorders was investigated in 274 sheep from 28 properties in the state of São Paulo, Brazil. Biological samples from 16 aborted fetuses, one uterus, six placentas, five uterine secretion samples, five vaginal swabs, 17 semen samples, and three preputial swabs were also subjected to bacterial isolation. The bacteria were isolated from fecal samples of 14.9% (5/28) of the properties, affecting 3.65% (10/274) of the sheep, 3.5% (9/255) of females and 5.3% (1/19) of males. Campylobacter jejuni was the most prevalent species, present in 66.67% (7) of the positive samples, followed by Campylobacter coli, present in 22.22% (2), and one strain was identified as Campylobacter spp. The birth of “weak” lambs (p=0.06, OR=6.83 and CI=1.73 to 27.05) and neonatal death (p=0.087, OR=3.5 and CI=0.83 to 14.72) were associated with the fecal isolation of Campylobacter spp. Diarrhea was also associated with the bacteria (p=0.003, OR=9.83 and CI=2.19 to 44.18). The dissemination of Campylobacter spp. in Brazilian sheep is low and that, at present, the existing strains are not responsible for significant economic losses in sheep production, especially in adult animals.


2012 ◽  
Vol 21 (4) ◽  
pp. 355-358 ◽  
Author(s):  
Sandra Valéria Inácio ◽  
Roberta Lomonte Lemos de Brito ◽  
Anaiza Simão Zucatto ◽  
Willian Marinho Dourado Coelho ◽  
Monally Conceição Costa de Aquino ◽  
...  

The present study aimed to analyze the occurrence of infection by Cryptosporidium spp. in mares and their respective foals. This study was carried out in 11 farms located in the municipalities of Araçatuba, Birigui, Guararapes and Santo Antônio do Aracangua, in the northwest region of the State of Sao Paulo, from November 2010 to March 2011. A total of 98 mares and 98 foals of several breeds were analyzed; among foals, 59 were males and 39 females, aged from three to 330 days. Feces were collected directly from the rectal ampulla, purified and processed according to modified Kinyoun stain. Occurrence of Cryptosporidium spp. was 21.4% (21/98) for foals and 18.4% (18/98) for mares. Occurrence of Cryptosporidium spp. had significant association with breeds and age of animals. Results obtained led to the conclusion that foals older than two months and Mangalarga animals are less susceptible to the occurrence of Cryptosporidium spp.


2011 ◽  
Vol 109 (3) ◽  
pp. 949-951 ◽  
Author(s):  
Marcelo V. Meireles ◽  
Fernando P. de Oliveira ◽  
Weslen Fabrício P. Teixeira ◽  
William M. D. Coelho ◽  
Luiz Cláudio N. Mendes

Author(s):  
Lilian Marques Silva

The almost instantaneous access to information provided by technological advances has revolutionized the behavior of people and of the classrooms too. Teachers had to adapt themselves to new technologies to maintain students interested and attentive to the discipline being taught. In this work, the behavior of the students of the 6th grade of elementary school II during class was observed. The school chosen is a public school in the State of São Paulo (Brazil). The research was based on data collection. The students were observed by being filmed during six months. The results showed that the students were interested in the classes and committed to the activities. The place that the student chooses to sit in the classroom influences the behavior of the teacher, because the more distant the teacher, the less he participates in the class.


Author(s):  
Leonardo Cardoso

This book is an ethnographic study of controversial sounds and noise control debates in Latin America’s most populous city. It discusses the politics of collective living by following several threads linking sound-making practices to governance issues. Rather than discussing sound within a self-enclosed “cultural” field, I examine it as a point of entry for analyzing the state. At the same time, rather than portraying the state as a self-enclosed “apparatus” with seemingly inexhaustible homogeneous power, I describe it as a collection of unstable (and often contradictory) sectors, personnel, strategies, discourses, documents, and agencies. My goal is to approach sound as an analytical category that allows us to access citizenship issues. As I show, environmental noise in São Paulo has been entangled in a wide range of debates, including public health, religious intolerance, crime control, urban planning, cultural rights, and economic growth. The book’s guiding question can be summarized as follows: how do sounds enter and leave the sphere of state control? I answer this question by examining a multifaceted process I define as “sound-politics.” The term refers to sounds as objects that are susceptible to state intervention through specific regulatory, disciplinary, and punishment mechanisms. Both “sound” and “politics” in “sound-politics” are nouns, with the hyphen serving as a bridge that expresses the instability that each concept inserts into the other.


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