scholarly journals Immunoprotection against influenza virus H9N2 by the oral administration of recombinant Lactobacillus plantarum NC8 expressing hemagglutinin in BALB/c mice

Virology ◽  
2014 ◽  
Vol 464-465 ◽  
pp. 166-176 ◽  
Author(s):  
Shao-Hua Shi ◽  
Wen-Tao Yang ◽  
Gui-Lian Yang ◽  
Yan-Long Cong ◽  
Hai-Bin Huang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Oral Administration ◽  
Lactobacillus Plantarum

scholarly journals Oral Administration of Lactobacillus plantarum Strain AYA Enhances IgA Secretion and Provides Survival Protection against Influenza Virus Infection in Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86416 ◽  
Author(s):  
Yosuke Kikuchi ◽  
Ayami Kunitoh-Asari ◽  
Katsuyuki Hayakawa ◽  
Shinjiro Imai ◽  
Kenji Kasuya ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Oral Administration ◽  
Lactobacillus Plantarum ◽  
Influenza Virus Infection

scholarly journals Augmentation of Cellular Immunity and Reduction of Influenza Virus Titer in Aged Mice Fed Lactobacillus casei Strain Shirota

2002 ◽  
Vol 9 (1) ◽  
pp. 105-108 ◽  
Author(s):  
Tetsuji Hori ◽  
Junko Kiyoshima ◽  
Kan Shida ◽  
Hisako Yasui
Keyword(s):  
Influenza Virus ◽  
Oral Administration ◽  
Cellular Immunity ◽  
Lactobacillus Casei ◽  
Control Diet ◽  
Killer Activity ◽  
Aged Mice ◽  
Tnf Α ◽  
Ifn Γ ◽  
Upper Respiratory

ABSTRACT We investigated whether oral administration of Lactobacillus casei strain Shirota activates the cellular immune system and ameliorates influenza virus (IFV) titer in the nasal site in upper respiratory IFV infection by using aged mice. Natural killer activity of splenocytes and lung cells of aged mice fed an L. casei strain Shirota diet (L.casei strain Shirota group) was significantly (P < 0.01 and P < 0.05) increased compared to those fed a control diet (control group). The increases were 1.5- and 2.5-fold, respectively. In aged mice fed an XL.casei strain Shirota diet, potent induction of gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α), which play a very important role in excluding IFV, was evident in nasal lymphocytes. IFN-γ and TNF-α production increased 12- and 3.5-fold, respectively. In this model of upper respiratory IFV infection, the titer of IFV in the nasal washings of aged mice fed an L.casei strain Shirota diet was significantly (P < 0.05) lower than that in aged mice fed a control diet (101.6 ± 0.6 and 102.2 ± 0.5, respectively). These findings suggest that oral administration of L.casei strain Shirota activates not only systemic cellular immunity but also local cellular immunity and that it ameliorates IFV infection.

Oral Administration of Lactobacillus plantarum HY7714 Protects Hairless Mouse Against Ultraviolet B-Induced Photoaging

2014 ◽  
Vol 24 (11) ◽  
pp. 1583-1591 ◽  
Author(s):  
Hyun Mee Kim ◽  
Dong Eun Lee ◽  
Soo Dong Park ◽  
Yong-Tae Kim ◽  
Yu Jin Kim ◽  
...  
Keyword(s):  
Oral Administration ◽  
Lactobacillus Plantarum ◽  
Hairless Mouse ◽  
Ultraviolet B

scholarly journals Protection against Influenza Virus Infection of Mice Fed Bifidobacterium breve YIT4064

1999 ◽  
Vol 6 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Hisako Yasui ◽  
Junko Kiyoshima ◽  
Tetuji Hori ◽  
Kan Shida
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Oral Administration ◽  
Lower Respiratory Tract ◽  
Influenza Virus Infection ◽  
Oral Immunization ◽  
Igg Antibodies ◽  
Virus Infections ◽  
Bifidobacterium Breve ◽  
Specific Igg

ABSTRACT Mice fed Bifidobacterium breve YIT4064 and immunized orally with influenza virus were more strongly protected against influenza virus infection of the lower respiratory tract than ones immunized with influenza virus only. The number of mice with enhanced anti-influenza virus immunoglobulin G (IgG) in serum upon oral administration of B. breve YIT4064 and oral immunization with influenza virus was significantly greater than that upon oral immunization with influenza virus only. These findings demonstrated that the oral administration of B. breve YIT4064 increased anti-influenza virus IgG antibodies in serum and protected against influenza virus infection. The oral administration of B. breve YIT4064 may enhance antigen-specific IgG against various pathogenic antigens taken orally and induce protection against various virus infections.

scholarly journals Dendritic Cells Targeting Lactobacillus plantarum Strain NC8 with a Surface-Displayed Single-Chain Variable Fragment of CD11c Induce an Antigen-Specific Protective Cellular Immune Response

2019 ◽  
Vol 88 (2) ◽  
Author(s):  
Jing Liu ◽  
Guilian Yang ◽  
Haibin Huang ◽  
Chunwei Shi ◽  
Xing Gao ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Influenza Virus ◽  
Virus Infection ◽  
Lactobacillus Plantarum ◽  
Influenza Virus Infection ◽  
Cell Culture Supernatant ◽  
Single Chain ◽  
Ifn Γ

ABSTRACT Influenza A virus (H1N1) is an acute, highly contagious respiratory virus. The use of lactic acid bacteria (LAB) to deliver mucosal vaccines against influenza virus infection is a research hot spot. In this study, two recombinant Lactobacillus plantarum strains expressing hemagglutinin (HA) alone or coexpressing aCD11c-HA to target HA protein to dendritic cells (DCs) by fusion to an anti-CD11c single-chain antibody (aCD11c) were constructed. The activation of bone marrow dendritic cells (BMDCs) by recombinant strains and the interaction of activated BMDCs and sorted CD4+ or CD8+ T cells were evaluated through flow cytometry in vitro, and cellular supernatants were assessed by using an enzyme-linked immunosorbent assay kit. The results demonstrated that, compared to the HA strain, the aCD11c-HA strain significantly increased the activation of BMDCs and increased the production of CD4+ gamma interferon-positive (IFN-γ+) T cells, CD8+ IFN-γ+ T cells, and IFN-γ in the cell culture supernatant in vitro. Consistent with these results, the aCD11c-HA strain clearly increased the activation and maturation of DCs, the HA-specific responses of CD4+ IFN-γ+ T cells, CD8+ IFN-γ+ T cells, and CD8+ CD107a+ T cells, and the proliferation of T cells in the spleen, finally increasing the levels of specific antibodies and neutralizing antibodies in mice. In addition, the protection of immunized mice was observed after viral infection, as evidenced by improved weight loss, survival, and lung pathology. The adoptive transfer of CD8+ T cells from the aCD11c-HA mice to NOD/Lt-SCID mice resulted in a certain level of protection after influenza virus infection, highlighting the efficacy of the aCD11c targeting strategy.

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