Changes in genomic imprinting and gene expression associated with transformation in a model of human osteosarcoma

Genomic imprinting is a form of non-Mendelian epigenetic inheritance that is defined by differential gene expression depending on its parental origin — maternal or paternal. It is known about 60 imprinted genes many of which effect significantly on the fetus growth and development. Methylation of DNA cytosine bases that defines the interaction of DNA and proteins identifying the modified bases and controls the gene expression through chromatin compacting-decompacting mechanism, is a main epigenetic genom modifier. Disturbances in monoallelic gene expression lead to the development of a special class of human hereditary diseases — genomic imprinting diseases.

Download Full-text

Functional Interference Between AP-1 and the Vitamin D Receptor on Osteocalcin Gene Expression in Human Osteosarcoma Cells

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1994.tb19989.x ◽

1994 ◽

Vol 224 (1) ◽

pp. 11-20 ◽

Cited By ~ 20

Author(s):

Tiina Jaaskelainen ◽

Asta Pirskanen ◽

Sanna Ryhanen ◽

Jorma J. Palvimo ◽

Hector F. Deluca ◽

...

Keyword(s):

Gene Expression ◽

Vitamin D ◽

Vitamin D Receptor ◽

Osteosarcoma Cells ◽

Human Osteosarcoma ◽

Functional Interference ◽

Human Osteosarcoma Cells ◽

Osteocalcin Gene

Download Full-text

DNA demethylase ROS1 negatively regulates the imprinting of DOGL4 and seed dormancy in Arabidopsis thaliana

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1812847115 ◽

2018 ◽

Vol 115 (42) ◽

pp. E9962-E9970 ◽

Cited By ~ 15

Author(s):

Haifeng Zhu ◽

Wenxiang Xie ◽

Dachao Xu ◽

Daisuke Miki ◽

Kai Tang ◽

...

Keyword(s):

Gene Expression ◽

Seed Dormancy ◽

Genomic Imprinting ◽

Dna Demethylation ◽

Paternal Allele ◽

Maternal Allele ◽

Imprinted Gene ◽

Differential Gene ◽

Parent Of Origin ◽

Dna Demethylase

Genomic imprinting is a form of epigenetic regulation resulting in differential gene expression that reflects the parent of origin. In plants, imprinted gene expression predominantly occurs in the seed endosperm. Maternal-specific DNA demethylation by the DNA demethylase DME frequently underlies genomic imprinting in endosperm. Whether other more ubiquitously expressed DNA demethylases regulate imprinting is unknown. Here, we found that the DNA demethylase ROS1 regulates the imprinting of DOGL4. DOGL4 is expressed from the maternal allele in endosperm and displays preferential methylation and suppression of the paternal allele. We found that ROS1 negatively regulates imprinting by demethylating the paternal allele, preventing its hypermethylation and complete silencing. Furthermore, we found that DOGL4 negatively affects seed dormancy and response to the phytohormone abscisic acid and that ROS1 controls these processes by regulating DOGL4. Our results reveal roles for ROS1 in mitigating imprinted gene expression and regulating seed dormancy.

Download Full-text

The discovery and importance of genomic imprinting

eLife ◽

10.7554/elife.42368 ◽

2018 ◽

Vol 7 ◽

Cited By ~ 13

Author(s):

Anne C Ferguson-Smith ◽

Deborah Bourchis

Keyword(s):

Gene Expression ◽

Genomic Imprinting ◽

Genetic Disorders ◽

Epigenetic Inheritance ◽

Specific Gene ◽

Specific Gene Expression ◽

Epigenetic Control ◽

International Award ◽

Parent Of Origin ◽

Shed Light

The discovery of genomic imprinting by Davor Solter, Azim Surani and co-workers in the mid-1980s has provided a foundation for the study of epigenetic inheritance and the epigenetic control of gene activity and repression, especially during development. It also has shed light on a range of diseases, including both rare genetic disorders and common diseases. This article is being published to celebrate Solter and Surani receiving a 2018 Canada Gairdner International Award "for the discovery of mammalian genomic imprinting that causes parent-of-origin specific gene expression and its consequences for development and disease".

Download Full-text