Inhibitory mechanisms of β-adrenoceptor agonists for immunoglobulin E-mediated experimental allergic reactions in rats

Abstract Background: The measurement of immunoglobulin E (IgE) in serum is widely used in the diagnosis of allergic reactions and parasitic infections. We describe here a fully automated assay for human IgE suitable for routine application in a general chemistry analyzer. Methods: We used an ILab 900® analyzer. This instrument automates a particle-enhanced immunoturbidimetric assay with an analysis time of 9 min. Results: The assay was linear in the range 4–1000 kIU/L (r = 0.9998). The intra- and interassay CVs at 57, 235, and 434 kIU/L were <3.5% and <7.4%, respectively. The detection limit was 4 kIU/L. Hemoglobin (≤16 g/L), bilirubin (≤250 μmol/L), and myeloma paraproteins did not interfere with the assay. The assay showed good correlation with a microparticle enzyme immunoassay (r = 0.998) with a mean difference between methods of −6 ± 26 kIU/L. Conclusion: The new automated serum assay for IgE is an attractive alternative that avoids the need for dedicated instrumentation.

Download Full-text

Ethanol Extract of Sesamum indicum Linn. Inhibits FcεRI-Mediated Allergic Reaction via Regulation of Lyn/Syk and Fyn Signaling Pathways in Rat Basophilic Leukemic RBL-2H3 Mast Cells

Mediators of Inflammation ◽

10.1155/2019/5914396 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8

Author(s):

Hyun Ju Do ◽

Tae Woo Oh ◽

Kwang-Il Park

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Signaling Pathways ◽

Inflammatory Mediators ◽

Cell Activation ◽

Immunoglobulin E ◽

Sesamum Indicum ◽

Mast Cell Activation ◽

Allergic Reactions ◽

Potent Effect

This study is aimed at determining whether Sesamum indicum Linn. beneficially influences FcεRI-mediated allergic reactions in RBL-2H3 mast cells; it is also aimed at further investigating Lyn/Fyn and Syk signaling pathways. To examine the antiallergic effect of Sesamum indicum Linn. extract (SIE), we treated antigen/immunoglobulin E- (IgE-) sensitized mast cells with extracts of various concentrations. We examined the degranulation release and concentrations of inflammatory mediators. Additionally, the expressions of genes involved in the FcεRI and arachidonate signaling pathways were examined. SIE inhibited the degranulation and secretion of inflammatory mediators in antigen/IgE-sensitized mast cells. SIE reduced the expressions of FcεRI signaling-related genes, such as Syk, Lyn, and Fyn, and the phosphorylation of extracellular signal-regulated kinase in antigen/IgE-sensitized mast cells. Additionally, in late allergic responses, SIE reduced PGD2 release and COX-2 and cPLA2 phosphorylation expression in FcεRI-mediated mast cell activation. Lastly, 250–500 mg/kg SIE significantly attenuated the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) reaction in mice. The potent effect of SIE on RBL-2H3 mast cell activation indicates that the extract could potentially be used as a novel inhibitor against allergic reactions.

Download Full-text

The effect of a thromboxane A2 receptor antagonist BAY-u-3405 on experimental allergic reactions

Prostaglandins ◽

10.1016/0090-6980(95)00111-5 ◽

1995 ◽

Vol 50 (2) ◽

pp. 75-87 ◽

Cited By ~ 22

Author(s):

Hiroichi Nagai ◽

Hiroshi Takeda ◽

Shuuji Yamaguchi ◽

Hiroyuki Tanaka ◽

Akihiko Matsuo ◽

...

Keyword(s):

Receptor Antagonist ◽

Thromboxane A2 ◽

Allergic Reactions ◽

Thromboxane A2 Receptor ◽

Experimental Allergic

Download Full-text

Markers showing allergic reactions in children caused by aerogenic exposure to manganese and nickel compounds

Health Risk Analysis ◽

10.21668/health.risk/2020.4.09 ◽

2020 ◽

pp. 84-91

Author(s):

S.L. Valina ◽

◽

I.E. Shtina ◽

O.A. Maklakova ◽

D.A. Eisfel'd ◽

...

Keyword(s):

School Children ◽

Immunoglobulin E ◽

Reference Group ◽

Background Level ◽

Close Relation ◽

Allergic Diseases ◽

Allergic Reactions ◽

Vegetative Nervous System ◽

Nickel Compounds ◽

Prevention Activities

High prevalence of allergic diseases among children and their close relation with quality of the environment require new approaches to organizing diagnostic and prevention activities. Our research objects were 247 children attending pre-school children facilities (PSCF). It was detected that children who permanently lived and/or attended a pre-school facility for not less than three years under long-term chemical aerogenic exposure to manganese and nickel compounds in low doses (0.17–0.23 MPC average daily) had contents of these metals in their blood that were 1.9–2.0 times higher than the same parameter in children from the reference group and 1.7–2.1 times higher than background level in the region. Pre-school children with their biological media being contaminated with nickel and manganese compounds suffered from atopic dermatitis, allergic rhinitis, and bronchial asthma 1.3–4.5 times more frequently (0.23R20.73; 59.2F388.1; р≤0.001). Allergic diseases associated with aerogenic exposure to chemicals with sensitizing power have certain pathogenetic peculiarities such as active overall inflammatory reaction; sensitization in 54–86% children (the parameter is 1.5–4.3 times higher than in the reference group); cellular metabolism disorder; depletion of antioxidant protection resources in 72% children; deficient activity of phagocytic and humoral section in immunity (1.2 times lower than in the reference group); cytokine regulation disorders (2.4–2.5 time difference); reduced expression of a receptor that induces activation apoptosis; stronger sympathetic influence on heart rate modulation in 26.0% children. Basing on statistical analysis and model making, we determined markers that showed occurring allergic reactions caused by aerogenic exposure to manganese and nickel compounds. These markers are targets for prevention activities; they include growth in allergic pathologies prevalence; these pathologies occurring together with chronic inflammatory-proliferative diseases and disorders in the vegetative nervous system; increased contents of leukocytes, eosinophils, and immunoglobulin E specific to nickel in blood; a decrease in phagocyte number and contents of IgM, IgА in blood serum (0.07≤R2≤0.74; 19.3≤F≤713.2; р≤0.0001).

Download Full-text

Determination of Salivary Myeloperoxidase, Immunoglobulin E, and Tumor Necrosis Factor-α after Complete Denture Insertion

Medical Principles and Practice ◽

10.1159/000499429 ◽

2019 ◽

Vol 28 (4) ◽

pp. 347-351 ◽

Cited By ~ 1

Author(s):

Milena Kostic ◽

Marko Igic ◽

Tatjana Jevtovic Stoimenov ◽

Ana Pejcic ◽

Jana Pesic Stankovic

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Immunoglobulin E ◽

Allergic Reactions ◽

Complete Dentures ◽

Observational Period ◽

Tnf Α ◽

Factor Α ◽

Necrosis Factor

Objective: To detect activities of salivary myeloperoxidase (MPO) and concentrations of salivary tumor necrosis factor (TNF)-α as indicators of inflammatory reaction and salivary immunoglobulin E as an indicator of allergic reaction after complete insertion of acrylic dentures. Subjects and Methods: Complete dentures were made for a uniform group of elderly patients, and saliva samples were taken immediately before they were given to the patients, as well as 2, 3, 7, and 30 days after insertion of the dentures, with simultaneous monitoring of changes in the oral mucosa. Results: After 7 and 30 days of wearing upper and lower complete dentures, nonsignificant increases in salivary MPO and TNF-α were proven to be indicators of inflammation. No changes were observed in the values of salivary immunoglobulin E during a 30-day observational period, which excluded the appearance of allergic reactions to acrylic materials in the tested group of patients. Conclusion: A nonsignificant increase in the levels of MPO was observed on day 7; it decreased after 30 days. TNF-α also tended to increase in a nonsignificant manner.

Download Full-text

Fenugreek Anaphylaxis in a Pediatric Patient

Allergy & Rhinology ◽

10.1177/2152656718764134 ◽

2018 ◽

Vol 9 ◽

pp. 215265671876413 ◽

Cited By ~ 1

Author(s):

Nancy I. Joseph ◽

Eileen Slavin ◽

Brian P. Peppers ◽

Robert W. Hostoffer

Keyword(s):

Pediatric Patient ◽

Immunoglobulin E ◽

Skin Testing ◽

Food Product ◽

Allergic Reactions ◽

True Nature ◽

Trigonella Foenum Graecum ◽

First Case ◽

Green Pea ◽

Ancient Times

Fenugreek ( Trigonella foenum-graecum) is a food product that belongs to the Leguminosae family along with other legumes. It has been used in India, Greece, and Egypt for culinary and medical purposes since ancient times, and today, fenugreek is used for flavoring foods, dyes, and drugs throughout the world. Many members of the Leguminosae family have been associated with allergies including soybean, green pea, and peanut. Fenugreek is also included in this family and may result in allergic reactions. Two cases of anaphylaxis have been described in children after ingestion of curry and pastes that contain fenugreek, although the true nature of the causative agent was unclear. We report the first case of fenugreek anaphylaxis in a pediatric patient defined by skin testing, immunoglobulin E ImmunoCAP assays, and clear ingestion.

Download Full-text

Clinical manifestations of immunoglobulin E‐mediated food allergy, including pollen‐food allergy syndrome

Journal of Food Allergy ◽

10.2500/jfa.2020.2.200002 ◽

2020 ◽

Vol 2 (1) ◽

pp. 22-25

Author(s):

Pooja Varshney ◽

Jacqueline A. Pongracic

Keyword(s):

Food Allergy ◽

Immunoglobulin E ◽

Acute Infection ◽

Clinical Manifestations ◽

Gastrointestinal Symptoms ◽

Physical Exertion ◽

Allergic Reactions ◽

Cutaneous Manifestations ◽

Ige Mediated ◽

Inflammatory Drugs

Immunoglobulin E-(IgE) mediated food allergy affects people of all ages but does not have a consistent presentation and may result in various manifestations, even for an individual. The onset of symptoms is usually quite rapid, minutes to a few hours after consumption of the allergen, although exceptions exist. Cutaneous and gastrointestinal symptoms are the most common clinical manifestations; however, they are not present in all allergic reactions. Clinicians, particularly those in emergency care settings, need to be aware that the lack of cutaneous manifestations does not exclude the possibility of anaphylaxis. It is extremely unusual for food allergy reactions to present with isolated upper or lower respiratory symptoms, nor is chronic urticaria a manifestation of food allergy. Clinical manifestations of IgE-mediated food allergy range from mild to severe and, in rare cases, can be fatal. Mild, localized reactions, such as those that occur in pollen‐food allergy syndrome, occur in individuals with sensitization to pollens. A small proportion of patients with this syndrome develop anaphylaxis. Alcohol, medications (nonsteroidal anti-inflammatory drugs, antacids), physical exertion, increased body temperature, acute infection, and menstruation are factors that are known to augment the severity of food-induced allergic reactions.

Download Full-text

ALLERGIC REACTIONS ASSOCIATED WITH AIRBORNE FISH PARTICLES IN IMMUNOGLOBULIN E (IgE)-MEDIATED FISH HYPERSENSITIVE PATIENTS

PEDIATRICS ◽

10.1542/peds.98.2.323 ◽

1996 ◽

Vol 98 (2) ◽

pp. 323-323

Author(s):

Robert S. Zeiger

Keyword(s):

Water Vapor ◽

Immunoglobulin E ◽

Allergic Reactions ◽

Airborne Particulates ◽

Ige Mediated

Fish allergens carried by fumes, water vapor, and airborne particulates cause respiratory and dermal reactions, occasionally severe, in up to 11% of IgE-mediated fish-allergic children.

Download Full-text

Effect of cinnarizine on IgE antibody-mediated experimental allergic reactions in guinea pigs

Allergy ◽

10.1111/j.1398-9995.1987.tb02371.x ◽

1987 ◽

Vol 42 (2) ◽

pp. 135-140 ◽

Cited By ~ 1

Author(s):

H. Nagai ◽

H. Yamada ◽

I. Yakuo ◽

N. Inagaki ◽

S. H. Choi ◽

...

Keyword(s):

Guinea Pigs ◽

Allergic Reactions ◽

Ige Antibody ◽

Experimental Allergic

Download Full-text

Amomum xanthiodes Inhibits Mast Cell-Mediated Allergic Reactions Through the Inhibition of Histamine Release and Inflammatory Cytokine Production

Experimental Biology and Medicine ◽

10.1177/153537020523000911 ◽

2005 ◽

Vol 230 (9) ◽

pp. 681-687 ◽

Cited By ~ 28

Author(s):

Sang-Hyun Kim ◽

Tae-Yong Shin

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Histamine Release ◽

Proinflammatory Cytokines ◽

Immunoglobulin E ◽

Ionophore A23187 ◽

Allergic Reactions ◽

Disodium Cromoglycate ◽

Plasma Histamine ◽

Intracellular Ca

In this study, we investigated the effect of Amomum xanthiodes (Zingiberaceae) extract (AXE) on the mast cell-mediated allergy model and studied the possible mechanism of action. We found that AXE inhibited compound 48/80-induced systemic reactions and plasma histamine release in mice. Additionally, AXE decreased immunoglobulin E (IgE)-mediated local allergic reactions and passive cutaneous anaphylaxis (PCA), and AXE dose-dependently attenuated the release of histamine from rat peritoneal mast cells (RPMC) activated by compound 48/80 or IgE. The amounts of AXE needed for inhibition of compound 48/80-induced plasma histamine release and PCA were similar to disodium cromoglycate, the known anti-allergic drug. We found that AXE increased the cAMP levels and decreased the compound 48/80-induced intracellular Ca2+. Furthermore, AXE attenuated the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (A23187)-stimulated tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 secretion in human mast cells. The inhibitory effect of AXE on the proinflammatory cytokines was nuclear factor-κB (NF-κB)-dependent. In addition, AXE decreased PMA plus A23187-induced degradation of IκBα and the nuclear translocation of NF-κB. Our findings provide evidence that AXE inhibits mast cell-derived immediate-type allergic reactions, and that cAMP, intracellular Ca2+, proinflammatory cytokines, and NF-κB are involved in these effects.

Download Full-text