scholarly journals Evaluation of a Particle-enhanced Turbidimetric Immunoassay for the Measurement of Immunoglobulin E in an ILab 900 Analyzer

1999 ◽  
Vol 45 (9) ◽  
pp. 1557-1561
Author(s):  
Sandra Guaita ◽  
Josep MA Simó ◽  
Natàlia Ferré ◽  
Jorge Joven ◽  
Jordi Camps
Keyword(s):  
General Chemistry ◽  
Detection Limit ◽  
Immunoglobulin E ◽  
Parasitic Infections ◽  
Attractive Alternative ◽  
Allergic Reactions ◽  
Automated Assay ◽  
Serum Assay ◽  
Microparticle Enzyme Immunoassay ◽  
Turbidimetric Immunoassay

Abstract Background: The measurement of immunoglobulin E (IgE) in serum is widely used in the diagnosis of allergic reactions and parasitic infections. We describe here a fully automated assay for human IgE suitable for routine application in a general chemistry analyzer. Methods: We used an ILab 900® analyzer. This instrument automates a particle-enhanced immunoturbidimetric assay with an analysis time of 9 min. Results: The assay was linear in the range 4–1000 kIU/L (r = 0.9998). The intra- and interassay CVs at 57, 235, and 434 kIU/L were <3.5% and <7.4%, respectively. The detection limit was 4 kIU/L. Hemoglobin (≤16 g/L), bilirubin (≤250 μmol/L), and myeloma paraproteins did not interfere with the assay. The assay showed good correlation with a microparticle enzyme immunoassay (r = 0.998) with a mean difference between methods of −6 ± 26 kIU/L. Conclusion: The new automated serum assay for IgE is an attractive alternative that avoids the need for dedicated instrumentation.

scholarly journals Ethanol Extract of Sesamum indicum Linn. Inhibits FcεRI-Mediated Allergic Reaction via Regulation of Lyn/Syk and Fyn Signaling Pathways in Rat Basophilic Leukemic RBL-2H3 Mast Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Hyun Ju Do ◽  
Tae Woo Oh ◽  
Kwang-Il Park
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Signaling Pathways ◽  
Inflammatory Mediators ◽  
Cell Activation ◽  
Immunoglobulin E ◽  
Sesamum Indicum ◽  
Mast Cell Activation ◽  
Allergic Reactions ◽  
Potent Effect

This study is aimed at determining whether Sesamum indicum Linn. beneficially influences FcεRI-mediated allergic reactions in RBL-2H3 mast cells; it is also aimed at further investigating Lyn/Fyn and Syk signaling pathways. To examine the antiallergic effect of Sesamum indicum Linn. extract (SIE), we treated antigen/immunoglobulin E- (IgE-) sensitized mast cells with extracts of various concentrations. We examined the degranulation release and concentrations of inflammatory mediators. Additionally, the expressions of genes involved in the FcεRI and arachidonate signaling pathways were examined. SIE inhibited the degranulation and secretion of inflammatory mediators in antigen/IgE-sensitized mast cells. SIE reduced the expressions of FcεRI signaling-related genes, such as Syk, Lyn, and Fyn, and the phosphorylation of extracellular signal-regulated kinase in antigen/IgE-sensitized mast cells. Additionally, in late allergic responses, SIE reduced PGD2 release and COX-2 and cPLA2 phosphorylation expression in FcεRI-mediated mast cell activation. Lastly, 250–500 mg/kg SIE significantly attenuated the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) reaction in mice. The potent effect of SIE on RBL-2H3 mast cell activation indicates that the extract could potentially be used as a novel inhibitor against allergic reactions.

Inhibitory mechanisms of β-adrenoceptor agonists for immunoglobulin E-mediated experimental allergic reactions in rats

1997 ◽  
Vol 336 (2-3) ◽  
pp. 225-231 ◽  
Author(s):  
Naoki Inagaki ◽  
Hirokazu Kawasaki ◽  
Hidetaka Hiyama ◽  
Moritaka Goto ◽  
Akihiko Matsuo ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Allergic Reactions ◽  
Inhibitory Mechanisms ◽  
Adrenoceptor Agonists ◽  
Experimental Allergic

scholarly journals An Unexpected Infection in Loss-of-function Mutations in STAT3: Malignant Alveolar Echinococcosis in Liver

2020 ◽  
Author(s):  
Sule Haskologlu ◽  
Figen Dogu ◽  
Gulnur Gollu Bahadır ◽  
Setenay Akyuzluer ◽  
Ergin Ciftci ◽  
...  
Keyword(s):  
Alveolar Echinococcosis ◽  
Immunoglobulin E ◽  
Fungal Infections ◽  
Parasitic Infections ◽  
Loss Of Function ◽  
Recurrent Pneumonia ◽  
Tissue Abnormalities ◽  
Transcription 3 ◽  
Slow Growing ◽  
Very High

Loss-of-function (LOF) mutations in signal transducer and activator of transcription 3 (STAT3) gene causes autosomal dominant hyper immunoglobulin E syndrome (AD-HIES or Job’s Syndrome), a rare and complex primary immunodeficiency (PID) syndrome characterized by increased levels of IgE (>2000 IU/mL), eosinophilia, recurrent staphylococcal skin abscesses, eczema, recurrent pneumonia, skeletal and connective tissue abnormalities. Although bacterial and fungal infections are common in AD-HIES, susceptibility to parasitic infections has not been reported. Alveolar echinococcosis (AE), a zoonosis caused by the growth of the Echinococcus multilocularis (EM) metacestode, mimics slow-growing liver cancer. The mortality rate of AE is very high when it is diagnosed late or under-treated. Here, we report a 14-year-old boy with AE infections of the liver and the lung resulting in liver failure and diagnosed as STAT3-LOF. To our knowledge, the association between these two conditions has not been reported in the literature before.

scholarly journals Markers showing allergic reactions in children caused by aerogenic exposure to manganese and nickel compounds

2020 ◽  
pp. 84-91
Author(s):  
S.L. Valina ◽  
◽  
I.E. Shtina ◽  
O.A. Maklakova ◽  
D.A. Eisfel'd ◽  
...  
Keyword(s):  
School Children ◽  
Immunoglobulin E ◽  
Reference Group ◽  
Background Level ◽  
Close Relation ◽  
Allergic Diseases ◽  
Allergic Reactions ◽  
Vegetative Nervous System ◽  
Nickel Compounds ◽  
Prevention Activities

High prevalence of allergic diseases among children and their close relation with quality of the environment require new approaches to organizing diagnostic and prevention activities. Our research objects were 247 children attending pre-school children facilities (PSCF). It was detected that children who permanently lived and/or attended a pre-school facility for not less than three years under long-term chemical aerogenic exposure to manganese and nickel compounds in low doses (0.17–0.23 MPC average daily) had contents of these metals in their blood that were 1.9–2.0 times higher than the same parameter in children from the reference group and 1.7–2.1 times higher than background level in the region. Pre-school children with their biological media being contaminated with nickel and manganese compounds suffered from atopic dermatitis, allergic rhinitis, and bronchial asthma 1.3–4.5 times more frequently (0.23R20.73; 59.2F388.1; р≤0.001). Allergic diseases associated with aerogenic exposure to chemicals with sensitizing power have certain pathogenetic peculiarities such as active overall inflammatory reaction; sensitization in 54–86% children (the parameter is 1.5–4.3 times higher than in the reference group); cellular metabolism disorder; depletion of antioxidant protection resources in 72% children; deficient activity of phagocytic and humoral section in immunity (1.2 times lower than in the reference group); cytokine regulation disorders (2.4–2.5 time difference); reduced expression of a receptor that induces activation apoptosis; stronger sympathetic influence on heart rate modulation in 26.0% children. Basing on statistical analysis and model making, we determined markers that showed occurring allergic reactions caused by aerogenic exposure to manganese and nickel compounds. These markers are targets for prevention activities; they include growth in allergic pathologies prevalence; these pathologies occurring together with chronic inflammatory-proliferative diseases and disorders in the vegetative nervous system; increased contents of leukocytes, eosinophils, and immunoglobulin E specific to nickel in blood; a decrease in phagocyte number and contents of IgM, IgА in blood serum (0.07≤R2≤0.74; 19.3≤F≤713.2; р≤0.0001).

scholarly journals A framework of host immune responses against four types of parasitic infections

2020 ◽  
Author(s):  
Wan-Chung Hu
Keyword(s):  
T Cells ◽  
B Cells ◽  
Mast Cells ◽  
Immune Responses ◽  
Cd4 T Cells ◽  
Immunoglobulin E ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Parasitic Infections ◽  
Host Immune Responses

Human host immune responses to parasitic infections are complex. They can be categorized into four immunological pathways against four types of parasitic infections. For intracellular protozoa, the eradicable host immunological pathway is TH1 immunity involving macrophages, interferon gamma (IFNg) CD4 T cells, innate lymphoid cells 1 (ILC1), CD8 T cells, invariant natural killer T cells 1 (iNKT1) cells, and immunoglobulin G3 (IgG3) B cells. For free-living extracellular protozoa, the eradicable host immunological pathway is TH22 immunity involving neutrophils, interleukin (IL)-22/IL-17 CD4 T cells, innate lymphoid cells 3 (ILC3), iNKT17 cells, and IgG2 B cells. For endoparasites (helminths), the eradicable host immunological pathway is TH2a immunity with inflammatory eosinophils (iEOS), IL-5/IL-4 CD4 T cells, IL-25 inducing inflammatory innate lymphoid cells 2 (iILC2), mast cells-tryptase (MCt), iNKT2 cells, and IgG4 B cells. For ectoparasites (parasitic insects and arachnids), the eradicable host immunological pathway is TH2b immunity with inflammatory basophils, mast cells-tryptase/chymase (MCtc), IL-3/IL-4 CD4 T cells, IL-33 inducing nature innate lymphoid cells 2 (nILC2), iNKT2 cells, and immunoglobulin E (IgE) B cells. The tolerable host immunity against ectoparasites and endoparasites is TH9 immunity with regulatory eosinophils, regulatory basophils, IL-9 mast cells (MMC9), thymic stromal lymphopoietin inducing innate lymphoid cells 2, IL-9 CD4 T cells, iNKT2 cells, and IgA2 B cells. This categorization provides a complete framework of immunological pathways against four types of parasitic infections.

scholarly journals Determination of Salivary Myeloperoxidase, Immunoglobulin E, and Tumor Necrosis Factor-α after Complete Denture Insertion

2019 ◽  
Vol 28 (4) ◽  
pp. 347-351 ◽  
Author(s):  
Milena Kostic ◽  
Marko Igic ◽  
Tatjana Jevtovic Stoimenov ◽  
Ana Pejcic ◽  
Jana Pesic Stankovic
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Immunoglobulin E ◽  
Allergic Reactions ◽  
Complete Dentures ◽  
Observational Period ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Objective: To detect activities of salivary myeloperoxidase (MPO) and concentrations of salivary tumor necrosis factor (TNF)-α as indicators of inflammatory reaction and salivary immunoglobulin E as an indicator of allergic reaction after complete insertion of acrylic dentures. Subjects and Methods: Complete dentures were made for a uniform group of elderly patients, and saliva samples were taken immediately before they were given to the patients, as well as 2, 3, 7, and 30 days after insertion of the dentures, with simultaneous monitoring of changes in the oral mucosa. Results: After 7 and 30 days of wearing upper and lower complete dentures, nonsignificant increases in salivary MPO and TNF-α were proven to be indicators of inflammation. No changes were observed in the values of salivary immunoglobulin E during a 30-day observational period, which excluded the appearance of allergic reactions to acrylic materials in the tested group of patients. Conclusion: A nonsignificant increase in the levels of MPO was observed on day 7; it decreased after 30 days. TNF-α also tended to increase in a nonsignificant manner.

Rapid, robust method for measuring low concentrations of albumin in urine

1990 ◽  
Vol 36 (3) ◽  
pp. 446-449 ◽  
Author(s):  
E A Medcalf ◽  
D J Newman ◽  
E G Gorman ◽  
C P Price
Keyword(s):  
Detection Limit ◽  
Correlation Coefficient ◽  
Robust Method ◽  
Low Concentrations ◽  
Analytical Recovery ◽  
Turbidimetric Immunoassay

Abstract We describe a rapid particle-enhanced turbidimetric immunoassay for albumin in urine. Intra- and interassay CVs were less than 5% and less than 10%, respectively, the detection limit is 2 mg/L, and the working range extends to 200 mg/L. Mean analytical recovery of albumin added to centrifuged urines was 100% (SD 10.6%), and, when results were compared with those by the Pharmacia RIA, the correlation coefficient was 0.99. The working reagents are stable for at least six months; thus this assay is suited for both batch and urgent analysis.

scholarly journals Fenugreek Anaphylaxis in a Pediatric Patient

2018 ◽  
Vol 9 ◽  
pp. 215265671876413 ◽  
Author(s):  
Nancy I. Joseph ◽  
Eileen Slavin ◽  
Brian P. Peppers ◽  
Robert W. Hostoffer
Keyword(s):  
Pediatric Patient ◽  
Immunoglobulin E ◽  
Skin Testing ◽  
Food Product ◽  
Allergic Reactions ◽  
True Nature ◽  
Trigonella Foenum Graecum ◽  
First Case ◽  
Green Pea ◽  
Ancient Times

Fenugreek ( Trigonella foenum-graecum) is a food product that belongs to the Leguminosae family along with other legumes. It has been used in India, Greece, and Egypt for culinary and medical purposes since ancient times, and today, fenugreek is used for flavoring foods, dyes, and drugs throughout the world. Many members of the Leguminosae family have been associated with allergies including soybean, green pea, and peanut. Fenugreek is also included in this family and may result in allergic reactions. Two cases of anaphylaxis have been described in children after ingestion of curry and pastes that contain fenugreek, although the true nature of the causative agent was unclear. We report the first case of fenugreek anaphylaxis in a pediatric patient defined by skin testing, immunoglobulin E ImmunoCAP assays, and clear ingestion.

Determination of Folate in Infant Formula and Adult/Pediatric Nutritional Formula by Optical Biosensor Assay: First Action 2011.05

2012 ◽  
Vol 95 (2) ◽  
pp. 298-300 ◽  
Author(s):  
Harvey Indyk Fonterra ◽  
Dawn Dowell
Keyword(s):  
Detection Limit ◽  
Infant Formula ◽  
Binding Protein ◽  
Optical Biosensor ◽  
Sensor Surface ◽  
Method Detection Limit ◽  
Folate Binding Protein ◽  
Automated Assay ◽  
Single Laboratory

Abstract After a review of data from a single-laboratory validation (SLV) study published in the International Dairy Journal 21, 783–789 (2011), a method for folate in infant formula and adult/pediatric nutritional formula was submitted for consideration of adoption by AOAC as an automated assay that is rapid and simple. The method uses an optical biosensor assay to quantitate total folate content in milk and milk-based pediatric and adult nutritional products. The assay uses folate binding protein and a functionalized sensor surface. The SLV showed an instrumental LOD of 0.1 ng/mL (equivalent to 2.5 μg/100 g for a typical infant formula). The method detection limit was 6.5 μg/100 g with a repeatability of 3.48% and an intermediate reproducibility of 4.63% RSD.

scholarly journals Is There a Clinical Significance of Very Low Serum Immunoglobulin E Level?

2021 ◽  
Author(s):  
Serdar Al ◽  
Suna Asilsoy ◽  
Nevin Uzuner ◽  
Gizem Atakul ◽  
Özge Atay ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Rank Order ◽  
Demographic Data ◽  
Autoimmune Disorders ◽  
High Serum ◽  
Serum Immunoglobulin ◽  
Parasitic Infections ◽  
Human Immune System ◽  
Immune Deficiencies ◽  
Auto Antibody

Abstract Purpose: It has been well known that high serum immunoglobulin (Ig) E levels are associated with allergies, parasitic infections and some immune deficiencies; however, the potential effects and clinical implications of low IgE level on the human immune system are not well known. To determine the disorders accompanying very low IgE levels in children and adults. Methods: The patients whose IgE levels were determined between January 2015 and September 2020 were analyzed, and the ones with an IgE level <2.5 IU/mL were included in the study. Demographic data, immunoglobulin levels, auto-antibody results, and the diagnoses of the patients were noted from the electronic recording system of the hospital. Result: The IgE levels were measured in 34,809 patients (21,875 children, 12,934 adults), and 180 patients had IgE levels <2.5 IU/mL. Eighty patients were children (0.37%), 100 were adults (0.77%). There was a malignant disease in 45 (11 of them children) (25%), autoimmune diseases in 30 (4 of them children) (16.7%) and immunodeficiency in 19 (16 of them children) (10.6%) of the patients. The most common reasons were other disases, immunodeficiency and malignancy in children; and malignancy, autoimmune disorders and other diseases in the adults, in rank order. The IgE level did not show any correlation with the levels of other immunoglobulins.Conclusion: Although rare, a low IgE level has been shown to accompany malignancies, autoimmune disorders and immune deficiencies. Patients with very low IgE levels should be carefully monitored for systemic disorders.

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