M1904 Downstream Signal Transduction Induced By Interleukin-1 Beta-Stimulated ROS Generation and Anti-Oxidative Effects of Quercetin-3-O-β-D-Glucuronopyranoside (QGC) in Feline Esophageal Epithelial Cells

2009 ◽  
Vol 136 (5) ◽  
pp. A-442-A-443
Author(s):  
Se Eun Lee ◽  
Hyeon Soo Jang ◽  
Hyun Ju Song ◽  
Wan Kyunn Hwang ◽  
Uy Dong Sohn
Keyword(s):  
Signal Transduction ◽  
Epithelial Cells ◽  
Interleukin 1 ◽  
Ros Generation ◽  
Interleukin 1 Beta ◽  
Esophageal Epithelial Cells ◽  
Oxidative Effects

scholarly journals Toll-like receptor signalling via IRAK4 confers epithelial integrity and tightness through regulation of junctional tension

2021 ◽  
Author(s):  
Jesse Peterson ◽  
Kinga Balogh Sivars ◽  
Ambra Bianco ◽  
Katja Roeper
Keyword(s):  
Signal Transduction ◽  
Epithelial Cells ◽  
Interleukin 1 ◽  
Toll Like Receptor ◽  
Receptor Signalling ◽  
Epithelial Junctions ◽  
Toll Receptor ◽  
Epithelial State ◽  
Tlr Signalling ◽  
Inhibitor Treatment

Toll-like receptors (TLRs) in mammalian systems are well characterised for their role in innate immunity. In addition, TLRs also fulfil crucial functions outside immunity, including the dorso-ventral patterning function of the original Toll receptor in Drosophila and neurogenesis in mice. Recent discoveries in flies suggested key roles for TLRs in epithelial cells in patterning of cytoskeletal activity near epithelial junctions. Here we address the function of TLRs and the downstream key signal transduction component IRAK4 (interleukin-1 receptor associated kinase 4) in human epithelial cells. Using differentiated human Caco-2 cells as a model for the intestinal epithelium, we show that these cells exhibit baseline TLR signalling as revealed by p-IRAK4 and that blocking IRAK4 function leads to a loss of epithelial tightness involving key changes at tight junctions and adherens junctions. These changes correlate with a loss of epithelial tension and changes in junctional actomyosin. Knock-down of IRAK4 and certain TLRs phenocopies the inhibitor treatment. These data suggest a model whereby TLR receptors near epithelial junctions might be involved in a continuous sensing of the epithelial state to promote epithelial tightness and integrity.

Comparison of Staining Methods to Detect Interleukin-1 Beta on Antigen Presenting Cells and Surface Epithelial Cells in the Tonsil

1999 ◽  
Vol 22 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Gretchen Frank ◽  
Janet Gorfien ◽  
Sandra Mendel ◽  
Peter D'Auria ◽  
Linda Brodsky ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Interleukin 1 ◽  
Antigen Presenting Cells ◽  
Interleukin 1 Beta ◽  
Staining Methods ◽  
Antigen Presenting
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