A METABOLIC STUDY OF AMYOTONIA CONGENITA

AbstractObjectivesDue to lack of adequate data on tramadol kinetic in relevance of CYP2D6 toxicity, this study was designed to investigate the effect of CYP2D6 phenotype in tramadol poisoning. The saliva, urine and blood samples were taken at the admission time. Consequently, concentration of tramadol and its major metabolites were measured.MethodsA pharmacokinetic and metabolic study was developed in cases of tramadol poisoned (n=96). Cases of tramadol poisoned evidenced seizure, hypertension, dizziness, nausea and vomiting symptoms participated.ResultsFemale cases showed higher N-desmethyltramadol (M2) tramadol concentrations than male cases: in urine (40.12 ± 124.53 vs. 7.3 ± 7.13), saliva (16.91 ± 26.03 vs. 5.89 ± 7.02), and blood (1.11 ± 1.56 vs. 0.3 ± 0.38) samples. Significant correlation between blood, saliva, and urine concentrations were found (r = 0.5). Based on the metabolic ratio of O-desmethyltramadol (M1) of male (0.53 ± 0.22) and female (0.43 ± 0.26), poisoning and severe symptoms like seizure in female occurs statistically fewer (13.04%) than in male (50.6%). Assessment of CYP2D6 phenotype showed all of the participants were extensive metabolizers (EM) and their phenotype was associated with clinical symptoms.ConclusionsAccording to our results, M1 as a high potent metabolite has an important role in toxicity and the likelihood of poisoning in people with EM phenotype. Finally, tramadol metabolic ratio may justify the cause of various symptoms in human tramadol poisoning.

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Insight into the Metabolic Profiles of Pb(II) Removing Microorganisms

Molecules ◽

10.3390/molecules26134008 ◽

2021 ◽

Vol 26 (13) ◽

pp. 4008

Author(s):

Carla Cilliers ◽

Evans M. N. Chirwa ◽

Hendrik G. Brink

Keyword(s):

Liquid Chromatography ◽

High Performance ◽

Microbial Consortium ◽

Luria Bertani ◽

Nutrient Concentrations ◽

Batch Experiments ◽

Metabolic Study ◽

Luria Bertani Broth ◽

Tandem Mass Spectroscopy ◽

Insight Into

The objective of the study was to gather insight into the metabolism of lead-removing microorganisms, coupled with Pb(II) removal, biomass viability and nitrate concentrations for Pb(II) bioremoval using an industrially obtained microbial consortium. The consortium used for study has proven to be highly effective at removing aqueous Pb(II) from solution. Anaerobic batch experiments were conducted with Luria-Bertani broth as rich growth medium over a period of 33 h, comparing a lower concentration of Pb(II) with a higher concentration at two different nutrient concentrations. Metabolite profiling and quantification were conducted with the aid of both liquid chromatography coupled with tandem mass spectroscopy (UPLC-HDMS) in a “non-targeted” fashion and high-performance liquid chromatography (HPLC) in a “targeted” fashion. Four main compounds were identified, and a metabolic study was conducted on each to establish their possible significance for Pb(II) bioremoval. The study investigates the first metabolic profile to date for Pb(II) bioremoval, which in turn can result in a clarified understanding for development on an industrial and microbial level.

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Metabolic Study of Ginsenoside Rg3 and Glimepiride in Type 2 Diabetic Rats by Liquid Chromatography Coupled with Quadrupole‐Orbitrap Mass Spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.9083 ◽

2021 ◽

Author(s):

Di Yang ◽

Xue Li ◽

Yunhua Fu ◽

Xingyu Tao ◽

Fei Zheng ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Diabetic Rats ◽

Ginsenoside Rg3 ◽

Metabolic Study ◽

Orbitrap Mass Spectrometry ◽

Type 2 Diabetic

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An antineoplastic C14-labeled methylhydrazine derivative in P815 mouse leukemia. a metabolic study

Cellular and Molecular Life Sciences ◽

10.1007/bf02297032 ◽

1965 ◽

Vol 21 (5) ◽

pp. 284-286 ◽

Cited By ~ 13

Author(s):

W. Kreis ◽

W. Yen

Keyword(s):

Metabolic Study ◽

Mouse Leukemia ◽

P815 Mouse

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A preliminary metabolic study of alpidem in rat and man

European Journal of Drug Metabolism and Pharmacokinetics ◽

10.1007/bf03189916 ◽

1987 ◽

Vol 12 (4) ◽

pp. 295-298 ◽

Cited By ~ 5

Author(s):

P. Padovani ◽

P. Guinebault ◽

S. Vajta ◽

A. Durand ◽

J. Allen ◽

...

Keyword(s):

Metabolic Study

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A GENETIC AND METABOLIC STUDY OF A FAMILY WITH HYPERLIPEMIA

PEDIATRICS ◽

10.1542/peds.29.4.566 ◽

1962 ◽

Vol 29 (4) ◽

pp. 566-578

Author(s):

George Bialkin ◽

Saul Zucker ◽

Burton S. Sklarin ◽

Kurt Hirschhorn ◽

Murray Davidson

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Lipid Levels ◽

Metabolic Study ◽

Diagnosis And Prognosis ◽

The Family ◽

Artery Disease ◽

Pathologic Physiology ◽

Relationship Of ◽

The Relationship

A family consisting of a mother and father, heterozygous for idiopathic hyperlipemia, and their four offspring, one heterozygous and three homozygous for the disease, are described. In addition, a fifth child who is heterozygous, born of same mother but by another incompletely studied father, is presented. The genetics of the disease in this family, and also in the general population, with emphasis on diagnosis and prognosis in heterozygotes is discussed. The interrelationship of various lipid components in serum and their metabolism are briefly reviewed. The possible defective mechanisms in hyperlipemia, the techniques for deciding on the specific defect, and their application to the members of this family are reviewed. The effect of heparin, nicotinic acid, and fat-free diets in the homozygous members of the family are evaluated and their therapeutic applications are discussed. The symptomatology, possible pathologic physiology, relationship to lipid levels in serum and occurrence of abdominal crises in some of the homozygous members of this family are pointed out. The relationship of cholesterol and triglyceride levels in serum to, and the significance of, idiopathic hyperlipemia in the genesis of, atherosclerosis and coronary artery disease is elucidated.

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