912 Dexamethasone inhibits gene expression and production of both TH1 and TH2 cytokines from peripheral blood mononuclear cells

1996 ◽  
Vol 97 (1) ◽  
pp. 410-410 ◽  
Author(s):  
D CHI ◽  
S SRIKANTH ◽  
K HALL ◽  
J SMITH ◽  
G KRISHNASWAMY
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Th2 Cytokines ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Th1 And Th2 Cytokines ◽  
Th1 And Th2

Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy

2015 ◽  
Author(s):  
Ling YANG ◽  
Yongxiang WANG ◽  
Xingshu MA ◽  
Songping WANG ◽  
Leying ZHANG
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Early Pregnancy ◽  
Peripheral Blood ◽  
Blood Circulation ◽  
Mononuclear Cells ◽  
Th2 Cytokines ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Th1 And Th2 Cytokines ◽  
Th1 And Th2

Conceptus-derived signals are involved in the maternal systemic immune regulation through blood circulation and lymph circulation by peripheral blood mononuclear cells (PBMC) in cattle. There exists a Th2-biased response in maternal uterus during early pregnancy in cattle. However, it is unclear that the changes in expression of Th1 and Th2 cytokines in PBMC during early pregnancy in cows. At present study, the expression of Th1 and Th2 cytokines in PBMC were explored at day 18 non-pregnant cows, day 18 and day 30 pregnant cows. Our results showed that IFN-?, was attenuated in PBMC, but TNF-a and IL-4 in PBMC were up-regulated during early pregnancy in cows. However, the member of the Th2 cytokines, including IL-4, IL-5, IL-6, IL-10 and IL-13, was augmented in day 30 pregnant PBMC. In conclusion, there existed a Th2-biased response in PBMC during early pregnancy, which was essential for successful pregnancy in cattle.

scholarly journals The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1773 ◽  
Author(s):  
Michela Alfarano ◽  
Donato Pastore ◽  
Vincenzo Fogliano ◽  
Casper Schalkwijk ◽  
Teresa Oliviero
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Limit Of Detection ◽  
Peripheral Blood Mononuclear ◽  
Potential Health ◽  
Age Related ◽  
Blood Mononuclear Cells ◽  
Expression Activity

Studies demonstrate that the potential health-beneficial effect of sulforaphane (SR), a compound formed in broccoli, is the result of a number of mechanisms including upregulation of phase two detoxification enzymes. Recent studies suggest that SR increases expression/activity of glyoxalase 1 (Glo1), an enzyme involved in the degradation of methylglyoxal, is major precursor of advanced glycation end products. Those compounds are associated with diabetes complications and other age-related diseases. In this study, the effect of SR on the expression/activity of Glo1 in peripheral blood mononuclear cells (PBMCs) from 8 healthy volunteers was investigated. PBMCs were isolated and incubated with SR (2.5 μM-concentration achievable by consuming a broccoli portion) for 24 h and 48 h. Glo1 activity/expression, reduced glutathione (GSH), and glutathione-S-transferase gene expression were measured. Glo1 activity was not affected while after 48 h a slight but significant increase of its gene expression (1.03-fold) was observed. GSTP1 expression slightly increased after 24 h incubation (1.08-fold) while the expressions of isoform GSTT2 and GSTM2 were below the limit of detection. GSH sharply decreased, suggesting the formation of GSH-SR adducts that may have an impact SR availability. Those results suggest that a regular exposure to SR by broccoli consumption or SR supplements may enhance Glo1.

Sign in / Sign up

Export Citation Format

Share Document