Amplification of DNA of Mycobacterium tuberculosis from peripheral blood of patients with pulmonary tuberculosis

The Lancet ◽  
1994 ◽  
Vol 344 (8917) ◽  
pp. 232-233 ◽  
Author(s):  
N.W. Schluger ◽  
R. Condos ◽  
S. Lewis ◽  
W.N. Rom
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Peripheral Blood

Detection of Mycobacterium tuberculosis DNA by PCR in Peripheral Blood of Patients with Pulmonary Tuberculosis

2007 ◽  
Vol 63 (4) ◽  
pp. 331
Author(s):  
Yoon Ki Hong ◽  
Kyung Uk Jo ◽  
Hyeyoung Lee ◽  
Mi-Na Kim ◽  
Heungsup Sung ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Peripheral Blood

scholarly journals PCR-Based Rapid Detection of Mycobacterium tuberculosis in Blood from Immunocompetent Patients with Pulmonary Tuberculosis

1998 ◽  
Vol 36 (10) ◽  
pp. 3094-3095 ◽  
Author(s):  
Niyaz Ahmed ◽  
Ashok Kumar Mohanty ◽  
Utpal Mukhopadhyay ◽  
Virender Kumar Batish ◽  
Sunita Grover
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Peripheral Blood ◽  
Rapid Detection ◽  
Insertion Sequence ◽  
Circulating Dna ◽  
Blood Samples ◽  
Pcr Test ◽  
Immunocompetent Patients

A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.

scholarly journals Subpopulation structure of regulatory Т-cells of blood in patients with multiple drugresistant pulmonary tuberculosis

2011 ◽  
Vol 10 (4) ◽  
pp. 183-187 ◽  
Author(s):  
Ye. G. Churina ◽  
V. V. Novitsky ◽  
O. I. Urazova ◽  
O. V. Voronkova ◽  
Yu. V. Kolobovnikova
Keyword(s):  
Drug Resistance ◽  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
T Lymphocytes ◽  
Regulatory T Cells ◽  
Pulmonary Tuberculosis ◽  
Immune Suppression ◽  
Peripheral Blood ◽  
Newly Diagnosed ◽  
Clinical Form

Subpopulation structure of regulatory T-cells of peripheral blood in patients with newly diagnosed pulmonary tuberculosis depending on the clinical form of disease and sensitivity of Mycobacterium tuberculosis to anti-tuberculosis drugs have been analyzed in this work. It has been shown that in formation immune suppression at infiltrative, dissemination and fibrosis-cavity pulmonary tuberculosis the leading part play natural regulatory CD4+ CD25+ Foxр3+ -T-lymphocytes. Thus increase of their number in blood at drug-resistance and drugsusceptible patients. It has been demonstrated that in patients with fibro-cavernous and infiltrative form of the disease and drug-resistance pulmonary tuberculosis the number of CD4+ CD25- Foxр3+ -regulatory T-cells were increasing.

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

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