Positive correlation between inhibitors of matrix metalloproteinase 1 and matrix metalloproteinases in malignant ovarian tumor tissues

1997 ◽  
Vol 120 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Fumitaka Kikkawa ◽  
Koji Tamakoshi ◽  
Akihiro Nawa ◽  
Kiyosumi Shibata ◽  
Sadako Yamagata ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Ovarian Tumor ◽  
Positive Correlation ◽  
Matrix Metalloproteinase 1 ◽  
Tumor Tissues ◽  
Malignant Ovarian Tumor

scholarly journals Reference ranges of matrix metalloproteinase-1, -2, -9 and tissue inhibitor of matrix metalloproteinases-1 concentrations in amniotic fluid in physiological pregnancy

2016 ◽  
Vol 62 (1) ◽  
pp. 96-98
Author(s):  
Yu.V. Korenovsky ◽  
O.V. Remneva
Keyword(s):  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Amniotic Fluid ◽  
Reference Values ◽  
Reference Ranges ◽  
Tissue Inhibitor ◽  
Protein Ratio ◽  
Matrix Metalloproteinase 1 ◽  
Term Labor ◽  
First Stage Of Labor

The aim of this study was to determine reference values of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in the amniotic fluid at the first stage of labor in physiological pregnancy. 89 women at the first stage of term labor have been examined. Samples of amniotic fluid were taken at the first period of labor by vaginal amniotomy. Concentrations ofMMP-1, MMP-2, MMP-9, and TIMP-1 were investigated in amniotic fluid by ELISA kits. We have determined normal concentration ranges for MMP-1, MMP-2, MMP-9, TIMP-1, and ratios of concentrations of MMPs and TIMP-1 (MMP-1/TIMP-1, MMP-2/TIMP-1, MMP-9/TIMP-1) in the amniotic fluid at the first period of labor in physiological pregnancy. These included: MMP-1 - 5.1-16.8 pg/mg of protein, MMP-2 - 238.3-374.1 pg/mg of protein, MMP-9 - 66.1-113.3 pg/mg of protein, TIMP-1 - 4.7-13.6 pg/mg of protein, ratio of MMP-1/TIMP-1 - 0.1-2.2, ratio of MMP-2/TIMP-1 - 19.9-55.7, ratio of MMP-9/TIMP-1 - 4.2-17.2.

scholarly journals Expressions of MMPs and TIMP-1 in Gastric Ulcers May DifferentiateH. pylori-Infected from NSAID-Related Ulcers

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Hsiu-Chi Cheng ◽  
Hsiao-Bai Yang ◽  
Wei-Lun Chang ◽  
Wei-Ying Chen ◽  
Yi-Chun Yeh ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Epithelial Cells ◽  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Immunohistochemical Staining ◽  
Gastric Ulcers ◽  
Matrix Metalloproteinase 1 ◽  
Two Factors ◽  
H Pylori

Background. Two major causes of gastric ulcers areHelicobacter pylori(H. pylori) infection and nonsteroidal anti-inflammatory drug (NSAID) use.Aims. This study aimed to determine if there were different expressions of matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) betweenH. pylori-infected and NSAID-related ulcers.Methods. The 126 gastric ulcer patients (H. pyloriinfectedn=46; NSAID relatedn=30; combined with two factorsn=50) provided ulcer and nonulcer tissues for assessment of MMP-3, -7, and -9 and TIMP-1 expression by immunohistochemical staining.Results. Gastric ulcer tissues had significantly higher MMP-3, -7, and -9 and TIMP-1 expressions than nonulcer tissues (P<0.05).H. pylori-infected gastric ulcers had even higher MMP-7, MMP-9, and TIMP-1 expressions in epithelial cells than NSAID-related gastric ulcers (P<0.05). In patients with the two combined factors, gastric ulcers expressed similar proportions of antral ulcers and MMP-7 and MMP-9 intensities to NSAID-related gastric ulcers, but lower MMP-9 and TIMP-1 thanH. pylori-infected gastric ulcers (P<0.05).Conclusions. H. pylori-infected gastric ulcers express higher MMP-7, MMP-9, and TIMP-1 than NSAID-related ulcers. In patients with the two combined factors, ulcer location and MMP-7 and MMP-9 intensities are similar to NSAID use.

scholarly journals Levels of Selected Matrix Metalloproteinases—MMP-1, MMP-2 and Fibronectin in the Saliva of Patients Planned for Endodontic Treatment or Surgical Extraction

2020 ◽  
Vol 9 (12) ◽  
pp. 3971
Author(s):  
Ewa Matuszczak ◽  
Izabela Cwalina ◽  
Marzena Tylicka ◽  
Katarzyna Wawrzyn ◽  
Magdalena Nowosielska ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Oral Cavity ◽  
Matrix Metalloproteinase ◽  
Dental Treatment ◽  
Tissue Injury ◽  
Soft Tissue Injury ◽  
Endodontic Treatment ◽  
Matrix Metalloproteinase 2 ◽  
Matrix Metalloproteinase 1 ◽  
Surgical Extraction

Objectives: Composition of saliva reflects the condition of the oral cavity. The aim of the study: Investigation of the concentrations of MMP-1 (Matrix metalloproteinase-1), MMP-2 (Matrix metalloproteinase-2) and fibronectin in the saliva of patients planned for endodontic treatment or surgical extraction. Material and methods: Seventy-five patients with caries and 14 healthy subjects were included in the study. Subjects were divided into group 1, in which 50 patients were planned for endodontic treatment, and group 2, in which 25 patients were planned for surgical extraction. For the measurements, we used a surface plasmon resonance imaging biosensor. Results: We found higher levels of MMP-1, MMP-2 and fibronectin in the saliva of patients planned for dental treatment than in healthy donors. We found lower concentrations of MMP-2 in subjects planned for surgical extraction, than in patients planned for endodontic treatment; however, there were no such differences in salivary concentrations of MMP-1 and fibronectin. There were no statistically significant differences in MMP-1 concentrations in the saliva before and after any type of dental treatment, but contrary to that, we found a statistically significant decrease in MMP-2 concentrations after endodontic treatment and after surgical extraction. We found a significant rise in the concentrations of fibronectin after surgical extraction but not after endodontic treatment. Conclusions: The concentrations of MMP-1 and MMP-2 in the saliva of our patients with caries were increased in comparison to healthy individuals, but after the treatment—so sanation of the oral cavity—we noted a decrease in matrix metalloproteinases (MMPs) levels. MMPs can be found in gingival crevicular fluid and saliva, carious dentin and plaque. According to our observations, the main source of MMPs in patients with caries is probably carious dentin. Increase in the salivary levels of fibronectin (FN) after surgical extraction may be connected with soft tissue injury caused by surgical extraction. Our results are another example of the fact that higher salivary concentrations of MMP-1, MMP-2 and FN can reflect the health status of the oral cavity in patients with caries.

scholarly journals SIRT1 redresses the imbalance of tissue inhibitor of matrix metalloproteinase-1 and matrix metalloproteinase-9 in the development of mouse emphysema and human COPD

2013 ◽  
Vol 305 (9) ◽  
pp. L615-L624 ◽  
Author(s):  
Hongwei Yao ◽  
Jae-woong Hwang ◽  
Isaac K. Sundar ◽  
Alan E. Friedman ◽  
Michael W. McBurney ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Transgenic Mice ◽  
Matrix Metalloproteinase ◽  
Molecular Mechanisms ◽  
Chronic Obstructive ◽  
Tissue Inhibitor ◽  
Obstructive Pulmonary Disease ◽  
Matrix Metalloproteinase 1 ◽  
Copd Patients ◽  
Lysine Residues

Sirtuin1 (SIRT1), a protein/histone deacetylase, protects against the development of pulmonary emphysema. However, the molecular mechanisms underlying this observation remain elusive. The imbalance of tissue inhibitor of matrix metalloproteinases (TIMPs)/matrix metalloproteinases (MMPs) plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD)/emphysema. We hypothesized that SIRT1 protects against emphysema by redressing the imbalance between MMPs and TIMPs. To test this hypothesis, SIRT1-deficient and overexpressing/transgenic mice were exposed to cigarette smoke (CS). The protein level and activity of MMP-9 were increased in lungs of SIRT1-deficient mice exposed to CS compared with wild-type (WT) littermates, and these effects were attenuated by SIRT1 overexpression. SIRT1 deficiency decreased the level of TIMP-1, which was augmented in SIRT1 transgenic mice compared with WT littermates by CS. However, the level of MMP-2, MMP-12, TIMP-2, TIMP-3, or TIMP-4 was not altered by SIRT1 in response to CS exposure. SIRT1 reduction was associated with imbalance of TIMP-1 and MMP-9 in lungs of smokers and COPD patients. Mass spectrometry and immunoprecipitation analyses revealed that TIMP-1 acetylation on specific lysine residues was increased, whereas its interaction with SIRT1 and MMP-9 was reduced in mouse lungs with emphysema, as well as in lungs of smokers and COPD patients. SIRT1 deficiency increased CS-induced TIMP-1 acetylation, and these effects were attenuated by SIRT1 overexpression. These results suggest that SIRT1 protects against COPD/emphysema, in part, via redressing the TIMP-1/MMP-9 imbalance involving TIMP-1 deacetylation. Thus redressing the TIMP-1/MMP-9 imbalance by pharmacological activation of SIRT1 is an attractive approach in the intervention of COPD.

Serum matrix metalloproteinases and tympanosclerosis

2010 ◽  
Vol 125 (2) ◽  
pp. 142-146 ◽  
Author(s):  
H Aslan ◽  
M Sinan Başoğlu ◽  
B Şentürk ◽  
C Özbay ◽  
H Katilmiş ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Otitis Media ◽  
Disease Process ◽  
Chronic Otitis Media ◽  
Control Group ◽  
Success Rates ◽  
Tissue Inhibitor ◽  
Matrix Metalloproteinase 1 ◽  
Significant Difference

AbstractAim:To investigate levels of matrix metalloproteinases 2 and 9, and of their tissue inhibitor (i.e. tissue inhibitor matrix metalloproteinase 1), in the serum of patients with tympanosclerosis.Materials and method:We included 40 patients (age range 13–63 years) who had undergone surgery in the ENT department of İzmir Atatürk Training and Research Hospital between 2002 and 2007. Twenty had uncomplicated chronic otitis media and 20 had tympanosclerosis. We also included as the control group 20 individuals with no history of previous otic complaints or systemic or infectious disease. Serum levels of serum matrix metalloproteinases 2 and 9 and of tissue inhibitor matrix metalloproteinase 1 were measured in all subjects and compared.Result:Significantly higher levels of serum matrix metalloproteinases 2 and 9 were found in the tympanosclerosis group, compared with the chronic otitis media and control groups. There was no statistically significant difference in tissue inhibitor matrix metalloproteinase 1 level between the three groups.Conclusion:Tympanosclerosis surgery has poor success rates, since the pathological process is still active. We suggest that high levels of matrix metalloproteinases may play a role in the continuation of the disease process.

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