Somatic embryogenesis and organogenesis from immature embryo cotyledons of three sour cherry cultivars (Prunus cerasus L.)

2000 ◽  
Vol 83 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Haoru Tang ◽  
Zhenglong Ren ◽  
Gabi Krczal
2021 ◽  
Vol 22 (10) ◽  
pp. 5310
Author(s):  
Eduardo Luján-Soto ◽  
Vasti T. Juárez-González ◽  
José L. Reyes ◽  
Tzvetanka D. Dinkova

MicroRNAs (miRNAs) are small non-coding RNAs that regulate the accumulation and translation of their target mRNAs through sequence complementarity. miRNAs have emerged as crucial regulators during maize somatic embryogenesis (SE) and plant regeneration. A monocot-specific miRNA, mainly accumulated during maize SE, is zma-miR528. While several targets have been described for this miRNA, the regulation has not been experimentally confirmed for the SE process. Here, we explored the accumulation of zma-miR528 and several predicted targets during embryogenic callus induction, proliferation, and plantlet regeneration using the maize cultivar VS-535. We confirmed the cleavage site for all tested zma-miR528 targets; however, PLC1 showed very low levels of processing. The abundance of zma-miR528 slightly decreased in one month-induced callus compared to the immature embryo (IE) explant tissue. However, it displayed a significant increase in four-month sub-cultured callus, coincident with proliferation establishment. In callus-regenerated plantlets, zma-miR528 greatly decreased to levels below those observed in the initial explant. Three of the target transcripts (MATE, bHLH, and SOD1a) showed an inverse correlation with the miRNA abundance in total RNA samples at all stages. Using polysome fractionation, zma-miR528 was detected in the polysome fraction and exhibited an inverse distribution with the PLC1 target, which was not observed at total RNA. Accordingly, we conclude that zma-miR528 regulates multiple target mRNAs during the SE process by promoting their degradation, translation inhibition or both.


Author(s):  
Lise Hansted ◽  
Brian W.W. Grout ◽  
Jergen Eilenberg ◽  
Ivar B. Dencker ◽  
Torben B. Todam-Andersen

2010 ◽  
Vol 16 (3) ◽  
Author(s):  
N. Papp ◽  
J. Nyéki ◽  
Z. Szabó ◽  
É. Stefanovits-Bányai ◽  
T. Szabó ◽  
...  

Sour cherry (Prunus cerasus L.) cultivars grown in Hungary are of local origin while most sweet cherry cultivars in Hungary are introduced from other countries.A great phenotypic variability is displayed by both species. In the present study, we analyzed 10 sour and 9 sweet cherry cultivars for their antioxidant capacity, total polyphenolics (TPC) and total anthocyanin (TMAC) contents. In general, sour cherries showed higher levels of antioxidant capacity, TPC and TMAC. The anthocyanin contents varied from 0.16 to 6.85 and 1.41 to 127.56 mg/100 g for sweet and sour cherries, respectively. However, TMAC generally seems to have a limited influence on the antioxidant capacity of cherries.An amarelletype sour cherry, ‘Pipacs 1’ showed the highest antioxidant capacity (21.21mmolAA/l) and TPC (44.07mgGA/l) in contrast to its lowanthocyanin content. The detected diversity presents a choice that can satisfy different consumer preferences, and meet specific nutritional requirements.


2012 ◽  
Vol 64 (2) ◽  
pp. 63-68 ◽  
Author(s):  
Stanisław Wociór ◽  
Irena Wójcik ◽  
Salwina Palonka

The present study, carried out in the period 2007-2009 in the Sandomierz Upland, did not show a significant effect of foliar fertilization on trunk thickness and canopy volume in the cultivar Łutówka. However, under the influence of urea applied three times in combination with Florovit at a concentration of 0.5%, a clear increasing trend was observed in the above mentioned parameters used to assess the strength of growth. In years of abundant fruiting, this study showed a significant increase, or on the verge of significance, in yield after threefold application of urea and Florovit in combination. A small insignificant effect of fertilization on fruit weight shows that the growth in yield after the application of fertilization was produced as a consequential effect of a significant increase in the length of one-year shoots and thereby in flowering intensity of the 'Łutówka' cherry trees. In the case of the sour cherry cultivar Łutówka bearing fruit on one-year shoots, foliar feeding after flowering using multi-nutrient fertilizers and urea can be recommended as a technological treatment, in particular in years of abundant fruiting.


2011 ◽  
Vol 17 (1-2) ◽  
Author(s):  
P. T. Nagy ◽  
J. Nyéki ◽  
M. Soltész ◽  
T. Szabó ◽  
Z. Szabó

Effect of the foliar boron (B) application on nutrient uptake and fruit quality of tart cherry (Prunus cerasus L.) was investigated from 2008 to 2009 on mature four favourite Hungarian tart cherry cultivars like ‘Oblacsinszka’, ‘Érdi bôtermő’, ‘Újfehértói fürtös’ and ‘Petri’. Tart cherry trees grown in Eastern Hungary (Újfehértó) on an acidic sandy soil with low B content. Trees were treated with B by foliar application (0.5% B) at full bloom and untreated with B served as a control. B sprays strongly affected on B content of inflorescences. However, B sprays had no consistent effect on summer leaf B status. In our experiment stronger year effect was observed at leaf B status than flower B status. The effectiveness of early spring boron applications are limited and mostly affected the flower B status only. Moreover, treatments had inconsistent effect on studied fruit inner parameters. Monosaccharides, vitamin C and organic acid contents of sour cherry were stronger affected by cultivars than applied treatments.


2017 ◽  
Vol 16 (6) ◽  
pp. 99-109
Author(s):  
Robert Kurlus ◽  
Sławomir Świerczyński ◽  
Krzysztof Rutkowski ◽  
Henryk Ratajkiewicz ◽  
Agnieszka Malinowska ◽  
...  

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