Sour cherry (Prunus cerasus) seed extract increases heme oxygenase-1 expression and decreases proinflammatory signaling in peripheral blood human leukocytes from rheumatoid arthritis patients

2014 ◽  
Vol 20 (1) ◽  
pp. 188-196 ◽  
Author(s):  
Fadia Mahmoud ◽  
David Haines ◽  
Rana Al-Awadhi ◽  
Ali A. Dashti ◽  
Adel Al-Awadhi ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Heme Oxygenase ◽  
Peripheral Blood ◽  
Sour Cherry ◽  
Seed Extract ◽  
Prunus Cerasus ◽  
Heme Oxygenase 1 ◽  
Human Leukocytes

scholarly journals Heme oxygenase-1 is induced in peripheral blood mononuclear cells of patients with acute pancreatitis: a potential therapeutic target

2011 ◽  
Vol 300 (1) ◽  
pp. G12-G20 ◽  
Author(s):  
Aida Habtezion ◽  
Raymond Kwan ◽  
Alice L. Yang ◽  
Maureen E. Morgan ◽  
Ehsaan Akhtar ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Heme Oxygenase ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Therapeutic Benefit ◽  
Heme Oxygenase 1 ◽  
Potential Therapeutic Target ◽  
Peripheral Blood Mononuclear ◽  
Mouse Splenocytes

Heme oxygenase-1 (HO-1) induction by hemin or Panhematin protects against experimental pancreatitis. As a preclinical first step toward determining whether HO-1 upregulation is a viable target in acute pancreatitis (AP) patients, we tested the hypothesis that HO-1 expression in peripheral blood mononuclear cell (PBMC) subsets of hospitalized patients with mild AP is upregulated then normalizes upon recovery and that cells from AP patients have the potential to upregulate their HO-1 ex vivo if exposed to Panhematin. PBMCs were isolated on days 1 and 3 of hospitalization from the blood of 18 AP patients, and PMBC HO-1 levels were compared with PMBCs of 15 hospitalized controls (HC) and 7 volunteer healthy controls (VC). On day 1 of hospitalization, AP patients compared with VCs had higher HO-1 expression in monocytes and neutrophils. Notably, AP monocyte HO-1 levels decreased significantly upon recovery. Panhematin induced HO-1 in ex vivo cultured AP PBMCs more readily than in HC or VC PBMCs. Furthermore, PBMCs from acutely ill AP patients on day 1 were more responsive to HO-1 induction compared with day 3 upon recovery. Similarly, mouse splenocytes had enhanced HO-1 inducibility as their pancreatitis progressed from mild to severe. In conclusion, AP leads to reversible PBMC HO-1 upregulation that is associated with clinical improvement and involves primarily monocytes. Leukocytes from AP patients or mice with AP are primed for HO-1 induction by Panhematin, which suggests that Panhematin could offer a therapeutic benefit.

scholarly journals Increased level of heme oxygenase-1 in rheumatoid arthritis synovial fluid

2011 ◽  
Vol 21 (2) ◽  
pp. 150-157 ◽  
Author(s):  
Ai Kitamura ◽  
Keiichiro Nishida ◽  
Takamitsu Komiyama ◽  
Hideyuki Doi ◽  
Yasutaka Kadota ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Rheumatoid Arthritis Synovial Fluid

scholarly journals Over-Expression of Heme Oxygenase-1 in Peripheral Blood Predicts the Progression and Relapse Risk of Chronic Myeloid leukemia

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5181-5181
Author(s):  
Jishi Wang ◽  
Sixi Wei ◽  
Yating Wang ◽  
Qixiang Chai ◽  
Qin Fang ◽  
...  
Keyword(s):  
Chronic Myeloid Leukemia ◽  
Heme Oxygenase ◽  
Peripheral Blood ◽  
Myeloid Leukemia ◽  
Linear Regression Analysis ◽  
Wilcoxon Test ◽  
Expression Level ◽  
Heme Oxygenase 1 ◽  
Cutoff Values ◽  
The Relationship

Abstract Background There are limited eligible clinical markers at present to monitor the progress of chronic myeloid leukemia (CML). Heme oxygenase-1 (HO-1), as one of the most important oxidation-regulating enzymes in vivo, suggests the onset and progression of cancer when highly expressed. Furthermore, HO-1 level is related with the occurrence and development of hematological diseases. But the relationship between HO-1 expression and progression/relapse of CML has seldom been studied hitherto. This study aimed to investigate the relationship between them to find out a new molecular marker for prediction. Methods A total of 60 peripheral blood and bone marrow (BM) samples from 25 CML patients in different phases were collected respectively to detect the expressions of HO-1 and bcr/abl using real-time PCR. Routine blood test was performed to detect the changes of leukocyte and platelet counts. The proportion of primitive cells in BM was detected by flow cytometry. The relationship between high HO-1 expression and CML progression and relapse was explored by the analysis of variance by Wilcoxon test and linear regression analysis. The diagnostic accuracy and cutoff values were determined by receiver operating characteristic curve. Results Relative expression of HO-1 mRNA in CML patients peripheral blood was significantly higher than that of donors (P <0.0001), which were 0.57±3.78 and (1.417±1.125)×10–6, respectively. HO-1 expression level in CML patients was 0.061 5±0.062 4, which decreased to 0.009 4±0.006 7 upon CMoR, and remained remarkably higher 0.016 3±0.017 5 than that of normal donors (1.417±1.125)×10–6, P <0.001. When relapse occurred, HO-1 expression significantly increased from 0.020 6±0.021 0 to 3.852±10.285 in CMoR stage and undergoing relapse. According to progression of CML, HO-1 expression level in CML patients increased from CP (0.009 5±0.017 6) to AP (0.028 0±0.055 7) and then to BP (0.276 7± 0.447 0) . And there was a linear correlation between HO-1 expression and proportion of primitive CML cells. The diagnostic accuracies and cutoff values of HO-1 expression for CML-CP, CML-AP, and CML-BP were 1.0, 0.748, and 0.965, respectively, as well as 0.000 070, 0.001 917, and 0.020 696, respectively. Conclusion HO-1 may be a potential molecular indicator for the progression and relapse of CML. Disclosures No relevant conflicts of interest to declare.

scholarly journals Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Tong-ling Yuan ◽  
Jin Chen ◽  
Yan-li Tong ◽  
Yan Zhang ◽  
Yuan-yuan Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankylosing Spondylitis ◽  
Bone Metabolism ◽  
Bone Remodeling ◽  
Heme Oxygenase ◽  
Serum Levels ◽  
Heme Oxygenase 1 ◽  
Tartrate Resistant Acid Phosphatase ◽  
Trap 5B ◽  
Potential Biomarkers

Backgrounds. Heme oxygenase-1 (HO-1) has been reported to play a regulatory role in osteoclastogenesis. Bone morphogenetic protein (BMP) pathways induce osteoblastic differentiation and bone remodeling.Aims. To identify serum levels of HO-1, BMP-7, and Runt related-transcription factor 2 (Runx2) in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS) and to investigate the relationships between HO-1, BMP-7, Runx2, and other common biomarkers for bone metabolism.Results. Serum levels of HO-1 and BMP-7 were revealed to be significantly higher in patients with RA or AS than in healthy controls (p<0.01). In RA group, HO-1 was positively correlated with BMP-7, Runx2, and tartrate-resistant acid phosphatase-5b (TRAP-5b) (p<0.05, resp.), BMP-7 was positively correlated with Runx2 and TRAP-5b (p<0.05, resp.), and Runx2 was negatively correlated with N-terminal midfragment of osteocalcin (NMID) (p<0.05). In AS group, we observed identical correlation between HO-1 and BMP-7, but opposite correlations between BMP-7 and TRAP-5b and between Runx2 and NMID, when comparing with the RA cohort.Conclusion. Our findings suggest that HO-1 and BMP-7 are potential biomarkers for bone metabolism in patients with RA and AS. The different correlations between the bone markers point to distinct differences in bone remodeling pathways in the two types of arthritis.

Expression of the antioxidant stress protein heme oxygenase-1 (HO-1) in human leukocytes

1999 ◽  
Vol 26 (1-2) ◽  
pp. 184-192 ◽  
Author(s):  
Andreas Michael Niess ◽  
Frank Passek ◽  
Ingrid Lorenz ◽  
Elisabeth Marion Schneider ◽  
Hans-Hermann Dickhuth ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Stress Protein ◽  
Heme Oxygenase 1 ◽  
Human Leukocytes ◽  
Antioxidant Stress
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