A quick and simple method for the identification of meat species and meat products by PCR assay

Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 bp), pig (254 bp), sheep (329 bp), beef (473 bp), chicken (612 bp) and duck (718 bp) in one reaction. The proposed method has been validated to be specific, sensitive, robust and inexpensive. Taken together, the developed septuple PCR assay is reliable and efficient, not only to authenticate animal species in commercial meat products, but also easily feasible in a general laboratory without special infrastructures.

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Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves’ Muntjac (Muntiacus reevesi micrurus)

Animals ◽

10.3390/ani11020426 ◽

2021 ◽

Vol 11 (2) ◽

pp. 426

Author(s):

Yun-Hsiu Hsu ◽

Wei-Cheng Yang ◽

Kun-Wei Chan

Keyword(s):

Species Identification ◽

Operating Time ◽

Meat Products ◽

Reaction System ◽

Lateral Flow ◽

Recombinase Polymerase Amplification ◽

Food Allergies ◽

Mitochondrial Cytochrome B ◽

Meat Species ◽

A New Technique

The identification of animal species of meat in meat products is of great concern for various reasons, such as public health, religious beliefs, food allergies, legal perspectives, and bushmeat control. In this study, we developed a new technique to identify Formosan Reeves’ muntjac in meat using recombinase polymerase amplification (RPA) in combination with a lateral flow (LF) strip. The DNA extracted from a piece of Formosan Reeves’ muntjac meat was amplified by a pair of specific primers based on its mitochondrial cytochrome b gene for 10 min at a constant temperature ranging from 30 to 45 °C using RPA. Using the specific probe added to the RPA reaction system, the amplified products were visualized on the LF strip within 5 min. The total operating time from quick DNA extraction to visualizing the result was approximately 30 min. The RPA-LF system we designed was efficient when using boiled, pan-fried, roasted, stir-fried, or stewed samples. The advantages of simple operation, speediness, and cost-effectiveness make our RPA-LF method a promising molecular detection tool for meat species identification of either raw or variously cooked Formosan Reeves’ muntjac meat. It is also possible to apply this method to identify the meat of other wildlife sources.

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Multispecies Identification of Oilseed- and Meat-Specific Proteins and Heat-Stable Peptide Markers in Food Products

Molecules ◽

10.3390/molecules26061577 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1577

Author(s):

Klaudia Kotecka-Majchrzak ◽

Natalia Kasałka-Czarna ◽

Agata Sumara ◽

Emilia Fornal ◽

Magdalena Montowska

Keyword(s):

Limit Of Detection ◽

Raw Materials ◽

Meat Products ◽

Food Products ◽

Plant Raw Materials ◽

Heat Stable ◽

2S Albumins ◽

Specific Proteins ◽

Meat Species ◽

Species Specific

Consumer demand for both plant products and meat products enriched with plant raw materials is constantly increasing. Therefore, new versatile and reliable methods are needed to find and combat fraudulent practices in processed foods. The objective of this study was to identify oilseed species-specific peptide markers and meat-specific markers that were resistant to processing, for multispecies authentication of different meat and vegan food products using the proteomic LC-MS/MS method. To assess the limit of detection (LOD) for hemp proteins, cooked meatballs consisting of three meat species and hemp cake at a final concentration of up to 7.4% were examined. Hemp addition at a low concentration of below 1% was detected. The LOD for edestin subunits and albumin was 0.9% (w/w), whereas for 7S vicilin-like protein it was 4.2% (w/w). Specific heat-stable peptides unique to hemp seeds, flaxseed, nigella, pumpkin, sesame, and sunflower seeds, as well as guinea fowl, rabbit, pork, and chicken meat, were detected in different meat and vegan foods. Most of the oilseed-specific peptides were identified as processing-resistant markers belonging to 11S globulin subunits, namely conlinin, edestin, helianthinin, pumpkin vicilin-like or late embryogenesis proteins, and sesame legumin-like as well as 2S albumins and oleosin isoforms or selected enzymic proteins.

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Anatomic features of horse and manchurian wapiti

BIO Web of Conferences ◽

10.1051/bioconf/20213606044 ◽

2021 ◽

Vol 36 ◽

pp. 06044

Author(s):

Nadezhda Momot ◽

Yulia Kolina ◽

Igor Kamliya ◽

Svetlana Terebova ◽

Tatiana Timofeeva

Keyword(s):

Laboratory Tests ◽

Meat Products ◽

The Body ◽

Anatomic Structure ◽

Illegal Hunting ◽

Mandatory Requirement ◽

Meat Species ◽

Parameters Analysis ◽

Meat Samples ◽

Organoleptic Parameters

Carrying out a sanitary and veterinary expertise is a mandatory requirement which is necessary for the admission of livestock products, meat in particular, to sale. When carrying veterinary and sanitary expertise we often come up the attempts of meat products adulteration, for example when livestock meat is replaced to wild one and vice versa. Most often such adulteration cases are the results of illegal hunting. The purpose of our work is study horse and Manchurian wapiti carcasses anatomic features. The main methods of meat species determine are analysis of carcass appearance, organoleptic parameters analysis, laboratory tests as well as analysis and feature examination of anatomic structure of the inspected carcass. To determine meat species we applied methods of comparative and anatomic examination, organoleptic parameters analysis of meat samples, and laboratory tests. The suggested methods of examination can be used not only for determination of the whole animal carcasses species, but for small parts of the body. It is of great importance in conducting forensic and veterinary researches, when the number of parts can be finite. Maximal efficiency can be achieved only with complex use of enumerated methods.

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MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products

Food Chemistry ◽

10.1016/j.foodchem.2019.01.007 ◽

2019 ◽

Vol 283 ◽

pp. 367-374 ◽

Cited By ~ 11

Author(s):

Katarzyna Nalazek-Rudnicka ◽

Ilona Kłosowska-Chomiczewska ◽

Andrzej Wasik ◽

Adam Macierzanka

Keyword(s):

Meat Products ◽

Meat Species

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Collaborative Trial for Validation of a Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay for Detection of Central Nervous System Tissues as Bovine Spongiform Encephalopathy RiskMaterial: Part 1

Journal of AOAC International ◽

10.1093/jaoac/89.5.1335 ◽

2006 ◽

Vol 89 (5) ◽

pp. 1335-1340

Author(s):

Amir Abdulmawjood ◽

Holger Schnenbrcher ◽

Michael BÜlte

Keyword(s):

Central Nervous System ◽

Polymerase Chain Reaction ◽

Real Time ◽

Meat Products ◽

Spongiform Encephalopathy ◽

Rt Pcr ◽

Chain Reaction ◽

Pcr Assay ◽

Heat Treated ◽

Polymerase Chain

Abstract A collaborative trial was conducted to evaluate a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection of central nervous system (CNS) tissues in meat products (e.g., sausages). The method is based on the detection of ruminant glial fibrillary acidic protein (GFAP) mRNA by applying real-time RT-PCR. The assay was evaluated through a multicenter trial involving 12 participating laboratories that received coded cDNA obtained from 3 different types of sausages. The participants used 5 different real-time detection systems. The results obtained in this validation revealed that this real-time RT-PCR assay performed well in the different laboratories with a detection limit of at least 0.1% CNS in those test materials that contained strongly heat-treated samples (sausages cooked at 120C) and the medium heat-treated samples (sausages cooked at 80C). The detection limit of liver sausages was determined to be 0.2% of CNS. Neither the samples with no CNS additive nor the bovine DNA and the negative control containing 100% swine brain gave any positive signals. The presented results indicate that the real-time RT-PCR assay was just as reproducible between laboratories, as repeatable within a laboratory, could reliably be used for detection of bovine spongiform encephalopathy risk material in meat and meat products, and signify that it may be used with confidence in any laboratory.

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