Changes in whole cell-derived fatty acids induced by benzene and occurrence of the unusual 16:1ω6c in Rhodococcus sp. 33

1999 ◽  
Vol 176 (1) ◽  
pp. 213-218 ◽  
Author(s):  
J Gutiérrez
Keyword(s):  
Fatty Acids ◽  
Whole Cell ◽  
Rhodococcus Sp

scholarly journals Amycolatopsis taiwanensis sp. nov., from soil

2006 ◽  
Vol 56 (8) ◽  
pp. 1811-1815 ◽  
Author(s):  
Min Tseng ◽  
Shu-Feng Yang ◽  
Wen-Jun Li ◽  
Cheng-Lin Jiang
Keyword(s):  
Fatty Acids ◽  
Soil Sample ◽  
Type Strain ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Whole Cell ◽  
Mycolic Acids ◽  
Cellular Fatty Acids ◽  
Actinomycete Strain

An actinomycete strain (0345M-7T) was isolated from a soil sample from Yilan county, Taiwan. The isolate displayed substrate mycelia, upon which were borne short spore chains. The spore chains were composed of non-motile, smooth-surfaced, oval spores. Strain 0345M-7T had meso-diaminopimelic acid in its peptidoglycan. Whole-cell sugars were galactose, glucose, arabinose and ribose. The only phospholipid found was phosphatidylethanolamine. The predominant menaquinone was MK-9(H4). Mycolic acids were not detected. Major cellular fatty acids were iso-C16 : 0 (38.1 %) and C17 : 1 (25.4 %). The DNA G+C content of strain 0345M-7T was 68.9 mol%. On the basis of phenotypic and genotypic data, it is proposed that strain 0345M-7T (=BCRC 16802T=KCTC 19116T) should be classified as the type strain of a novel species of the genus Amycolatopsis, Amycolatopsis taiwanensis sp. nov.

Altered sodium current response to intracellular fatty acids in halothane-hypersensitive skeletal muscle

1996 ◽  
Vol 271 (1) ◽  
pp. C347-C353 ◽  
Author(s):  
S. J. Wieland ◽  
Q. H. Gong ◽  
J. E. Fletcher ◽  
H. Rosenberg
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Sodium Current ◽  
Primary Cultures ◽  
Na Channel ◽  
Current Response ◽  
Whole Cell ◽  
Na Currents

Biopsies of human skeletal muscle were analyzed by an in vitro contracture test (IVCT) for responsiveness to a halothane challenge: noncontracting (nonresponsive; IVCT-) and contracting (IVCT+). A muscle biopsy that is IVCT+ indicates potential malignant hyperthermia (MH) susceptibility. Primary cultures were grown from portions of the skeletal muscle biopsies, and voltage-activated currents were measured by whole cell recording in the presence or absence of 2-5 microM intracellular arachidonic or oleic acids. In untreated IVCT- cells, Na+ currents were predominantly tetrodotoxin (TTX) insensitive, indicating that most of the current was carried through the embryonic SkM2 isoform of the Na+ channel. Inclusion of fatty acids in the recording pipette of IVCT- cells produced an increase in voltage-activated Na+ currents during 20 min of recording. Approximately 70% of currents in fatty acid-treated cells were TTX sensitive, indicating activation of the adult SkM1 isoform of the Na+ channel. In contrast to IVCT- cells, IVCT+ cells expressed Na+ currents that were predominantly TTX sensitive even in the absence of added fatty acid, thus showing a relatively large baseline functional expression of SkM1 channels. Addition of fatty acids to the recording pipette produced little further change in the magnitude or TTX sensitivity of the whole cell currents in IVCT+ cells, suggesting altered functional regulation of Na+ channels in MH muscle.

Whole cell catalyzed esterification of fatty acids to biodiesel usingAspergillussp.

2011 ◽  
Vol 29 (6) ◽  
pp. 354-358 ◽  
Author(s):  
Satnam S. Aulakh ◽  
Manmohan Chhibber ◽  
Rasika Mantri ◽  
Ranjana Prakash
Keyword(s):  
Fatty Acids ◽  
Whole Cell

Design and engineering of whole-cell biocatalytic cascades for the valorization of fatty acids

2020 ◽  
Vol 10 (1) ◽  
pp. 46-64 ◽  
Author(s):  
Ji-Won Song ◽  
Joo-Hyun Seo ◽  
Doek-Kun Oh ◽  
Uwe T. Bornscheuer ◽  
Jin-Byung Park
Keyword(s):  
Fatty Acids ◽  
Key Factors ◽  
Whole Cell

This review presents the key factors to construct a productive whole-cell biocatalytic cascade exemplified for the biotransformation of renewable fatty acids.

The effect of fatty acids on the metabolism of pyruvate in lactic acid streptococci

1970 ◽  
Vol 37 (3) ◽  
pp. 445-456 ◽  
Author(s):  
R. F. Anders ◽  
G. R. Jago
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Lactic Acid ◽  
Oleic Acid ◽  
Mechanism Of Action ◽  
Dairy Products ◽  
Volatile Acid ◽  
Whole Cell ◽  
Flavour Compounds ◽  
Ph Range

SummaryThe metabolism, of pyruvate by resting whole-cell suspensions of Group N streptococci was studied over the pH range 4·0–7·0, in the presence and absence of oleic acid. In the absence of oleic acid pyruvate was utilized maximally at pH 4·5 with the formation of acetate (volatile acid), acetoin + diacetyl and carbon dioxide. The formation of acetate took precedence over the formation of acetoin + diacetyl. In the presenoe of oleic acid the utilization of pyruvate was maximal at pH 6·5 and completely inhibited at pH 4·5. The only products detected at pH 6·5 were acetoin + diacetyl and carbon dioxide. This effect of oleic acid on the metabolism of pyruvate was also obtained after treating the cells with acetone. The mechanism of action of oleic acid on cells of Group N streptococci and its possible influence on the formation of flavour compounds in cultured dairy products is discussed.

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