Identification of differentially expressed mRNA species during HIV infection by RNA arbitrarily primed PCR

1996 ◽  
Vol 7 (1) ◽  
pp. 43-53
Author(s):  
V. Boyer ◽  
P. Pezzoli ◽  
G. Audoly ◽  
C. Desgranges ◽  
F. Jensen ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Differentially Expressed ◽  
Mrna Species ◽  
Differentially Expressed Mrna ◽  
Arbitrarily Primed Pcr

Hunting for Differentially Expressed mRNA Species in Preconditioned Myocardium

1996 ◽  
Vol 793 (1 Myocardial Pr) ◽  
pp. 240-258 ◽  
Author(s):  
NILANJANA MAULIK ◽  
RICHARD M. ENGELMAN ◽  
DIPAK K. DAS
Keyword(s):  
Differentially Expressed ◽  
Mrna Species ◽  
Differentially Expressed Mrna

scholarly journals Identification of differentially expressed mRNA species by an improved disply technique (DDRT-PCR)

1993 ◽  
Vol 21 (18) ◽  
pp. 4272-4280 ◽  
Author(s):  
David Bauer ◽  
Heiko Muüller ◽  
Jens Reich ◽  
Heidemarie Riedel ◽  
vibeke Ahrenkiel ◽  
...  
Keyword(s):  
Differentially Expressed ◽  
Mrna Species ◽  
Differentially Expressed Mrna

scholarly journals Integrated analysis of lncRNA, miRNA and mRNA profiles reveals potential lncRNA functions during early HIV infection

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Lianwei Ma ◽  
Hui Zhang ◽  
Yue Zhang ◽  
Hailong Li ◽  
Minghui An ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Immune Activation ◽  
Expression Profiles ◽  
Critical Role ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Target Mrnas ◽  
Immunodeficiency Virus ◽  
Mirna Sequencing ◽  
Hiv 1

Abstract Background Long noncoding RNAs (lncRNAs) can regulate gene expression in a cis-regulatory fashion or as “microRNA sponges”. However, the expression and functions of lncRNAs during early human immunodeficiency virus (HIV) infection (EHI) remain unclear. Methods 3 HAART-naive EHI patients and 3 healthy controls (HCs) were recruited in this study to perform RNA sequencing and microRNA (miRNA) sequencing. The expression profiles of lncRNAs, mRNAs and miRNAs were obtained, and the potential roles of lncRNAs were analysed based on discovering lncRNA cis-regulatory target mRNAs and constructing lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) networks. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on 175 lncRNA-associated differentially expressed (DE) mRNAs to investigate the potential functions of DE lncRNAs in ceRNA networks. Results A total of 242 lncRNAs, 1240 mRNAs and 21 mature known miRNAs were determined as differentially expressed genes in HAART-naive EHI patients compared to HCs. Among DE lncRNAs, 44 lncRNAs were predicted to overlap with 41 target mRNAs, and 107 lncRNAs might regulate their nearby DE mRNAs. Two DE lncRNAs might regulate their cis-regulatory target mRNAs BTLA and ZAP70, respectively, which were associated with immune activation. In addition, the ceRNA networks comprised 160 DE lncRNAs, 21 DE miRNAs and 175 DE mRNAs. Seventeen DE lncRNAs were predicted to regulate HIF1A and TCF7L2, which are involved in the process of HIV-1 replication. Twenty DE lncRNAs might share miRNA response elements (MREs) with FOS, FOSB and JUN, which are associated with both immune activation and HIV-1 replication. Conclusions This study revealed that lncRNAs might play a critical role in HIV-1 replication and immune activation during EHI. These novel findings are helpful for understanding of the pathogenesis of HIV infection and provide new insights into antiviral therapy.

scholarly journals lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7491 ◽  
Author(s):  
Haibin Zhang ◽  
Cheng Chen ◽  
Yinghong Cui ◽  
Yuqing Li ◽  
Zhaojun Wang ◽  
...  
Keyword(s):  
Biological Function ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Human Chondrocytes ◽  
Loss Of Function ◽  
Knee Oa ◽  
System Disease ◽  
Chondrocyte Death ◽  
Differentially Expressed Mrna ◽  
Gain Loss

Osteoarthritis (OA) is the most common motor system disease in aging people, characterized by matrix degradation, chondrocyte death, and osteophyte formation. OA etiology is unclear, but long noncoding RNAs (lncRNAs) that participate in numerous pathological and physiological processes may be key regulators in the onset and development of OA. Because profiling of lncRNAs and their biological function in OA is not understood, we measured lncRNA and mRNA expression profiles using high-throughput microarray to study human knee OA. We identified 2,042 lncRNAs and 2,011 mRNAs that were significantly differentially expressed in OA compared to non-OA tissue (>2.0- or < − 2.0-fold change; p < 0.5), including 1,137 lncRNAs that were upregulated and 905 lncRNAs that were downregulated. Also, 1,386 mRNA were upregulated and 625 mRNAs were downregulated. QPCR was used to validate chip results. Gene Ontology analysis and the Kyoto Encyclopedia of Genes and Genomes was used to study the biological function enrichment of differentially expressed mRNA. Additionally, coding-non-coding gene co-expression (CNC) network construction was performed to explore the relevance of dysregulated lncRNAs and mRNAs. Finally, the gain/loss of function experiments of lnc-SAMD14-4 was implemented in IL-1β-treated human chondrocytes. In general, this study provides a preliminary database for further exploring lncRNA-related mechnisms in OA.

scholarly journals MicroRNA dynamics during hibernation of the Australian central bearded dragon (Pogona vitticeps)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Alexander Capraro ◽  
Denis O‘Meally ◽  
Shafagh A. Waters ◽  
Hardip R. Patel ◽  
Arthur Georges ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Physiological State ◽  
Regulation Of Gene Expression ◽  
Differentially Expressed ◽  
Small Rna Sequencing ◽  
Tissue Specific ◽  
Mrna Targets ◽  
Bearded Dragon ◽  
Pogona Vitticeps ◽  
Differentially Expressed Mrna

Abstract Hibernation is a physiological state employed by many animals that are exposed to limited food and adverse winter conditions. Controlling tissue-specific and organism wide changes in metabolism and cellular function requires precise regulation of gene expression, including by microRNAs (miRNAs). Here we profile miRNA expression in the central bearded dragon (Pogona vitticeps) using small RNA sequencing of brain, heart, and skeletal muscle from individuals in late hibernation and four days post-arousal. A total of 1295 miRNAs were identified in the central bearded dragon genome; 664 of which were novel to central bearded dragon. We identified differentially expressed miRNAs (DEmiRs) in all tissues and correlated mRNA expression with known and predicted target mRNAs. Functional analysis of DEmiR targets revealed an enrichment of differentially expressed mRNA targets involved in metabolic processes. However, we failed to reveal biologically relevant tissue-specific processes subjected to miRNA-mediated regulation in heart and skeletal muscle. In brain, neuroprotective pathways were identified as potential targets regulated by miRNAs. Our data suggests that miRNAs are necessary for modulating the shift in cellular metabolism during hibernation and regulating neuroprotection in the brain. This study is the first of its kind in a hibernating reptile and provides key insight into this ephemeral phenotype.

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