Biotypes, Serovars and Antimicrobial Resistance Patterns of Acinetobacter baumannii Clinical Isolates

The development of multidrug-resistant Acinetobacter baumannii is of serious concern in the hospital setting. Here, we report draft genome sequences of 11 A. baumannii isolates that were isolated from a single patient over a 65-day period, during which time the isolates exhibited increased antimicrobial resistance.

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Clinical isolates ofMECA, methicillin, vancomycin resistanceS. aureus; ESBLs producingK. pneumonia,E. coli,P. auregenosafrom various clinical source and its antimicrobial resistance patterns

International Journal of Medical Research & Health Sciences ◽

10.5958/2319-5886.2015.00020.x ◽

2015 ◽

Vol 4 (1) ◽

pp. 123

Author(s):

Ismail Mahmud Ali ◽

R Amirthalingam

Keyword(s):

Antimicrobial Resistance ◽

Clinical Isolates ◽

E Coli ◽

Resistance Patterns ◽

Clinical Source

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Comparison of Genospecies and Antimicrobial Resistance Profiles of Isolates in the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex from Various Clinical Specimens

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01304-12 ◽

2012 ◽

Vol 56 (12) ◽

pp. 6267-6271 ◽

Cited By ~ 9

Author(s):

Ni Tien ◽

Bang-Jau You ◽

Hui-Lan Chang ◽

Hsiu-Shen Lin ◽

Chin-Yi Lee ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Acinetobacter Baumannii ◽

Restriction Analysis ◽

Acinetobacter Calcoaceticus ◽

Its Region ◽

Clinical Isolates ◽

23S Rrna ◽

Rrna Gene ◽

Genotype 3 ◽

Content Type

ABSTRACTThis study was conducted to compare the prevalences of antimicrobial resistance profiles of clinical isolates in theAcinetobacter calcoaceticus-Acinetobacter baumanniicomplex from sterile and nonsterile sites and to further study the relationship of antimicrobial resistance profiles and genospecies by amplified rRNA gene restriction analysis (ARDRA). A total of 1,381 isolates were tested with 12 different antibiotics to show their antimicrobial susceptibility profiles. A total of 205 clinical isolates were further analyzed by ARDRA of the intergenic spacer (ITS) region of the 16S-23S rRNA gene. It was found that the overall percentage of isolates from nonsterile sites (urine, sputum, pus, or catheter tip) that were resistant to the 12 antibiotics tested was significantly higher than that of isolates from sterile sites (cerebrospinal fluid [CSF], ascites fluid, and bloodstream) (46% versus 22%;P< 0.05). After ARDRA, it was found that 97% of the 62 isolates resistant to all antibiotics tested were theA. baumanniigenospecies, which was identified in only 31% of the isolates susceptible to all antibiotics tested. More genospecies diversity was identified in the isolates susceptible to all antibiotics tested, including genospecies of 13TU (34%), genotype 3 (29%), andA. calcoaceticus(5%). Furthermore, as 91% (10/11) of the isolates from CSF were susceptible to all antibiotics tested, theA. calcoaceticus-A. baumanniicomplex isolates with multidrug resistance could be less invasive than the more susceptible isolates. This study also indicated current emergence of carbapenem-, fluoroquinolone-, aminoglycoside-, and cephalosporin-resistantA. calcoaceticus-A. baumanniicomplex isolates in Taiwan.

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Carbapenem-susceptible Acinetobacter baumannii carrying the ISAba1 upstream blaOXA-51-like gene in Porto Alegre, southern Brazil

Epidemiology and Infection ◽

10.1017/s095026881200074x ◽

2012 ◽

Vol 141 (2) ◽

pp. 330-333 ◽

Cited By ~ 14

Author(s):

M. PAGANO ◽

A. F. MARTINS ◽

A. B. M. P. MACHADO ◽

J. BARIN ◽

A. L. BARTH

Keyword(s):

Antimicrobial Resistance ◽

Acinetobacter Baumannii ◽

Clinical Isolates ◽

High Morbidity ◽

Southern Brazil ◽

Porto Alegre ◽

Integrase Gene ◽

Carbapenem Resistant ◽

High Prevalence ◽

Class 1

SUMMARYOver the last decade, Acinetobacter baumannii resistant to carbapenems has emerged in many medical centres and is commonly associated with high morbidity and mortality. We investigated potential mechanisms contributing to antimicrobial resistance of 58 clinical isolates of A. baumannii collected during a prolonged city-wide outbreak in five different hospitals in southern Brazil. The integrase gene was detected in 51 (87·9%) isolates of which 36 harboured class 2 integrons alone and 14 had both class 1 and 2 integrons; all carbapenem-resistant isolates displayed class 2 integrons. ISAba1 was found upstream of blaOXA-23-like only in isolates resistant to carbapenems; however, ISAba1 upstream of blaOXA-51-like was present in both susceptible and resistant isolates. This is the first report of a high prevalence of class 2 integrons in A. baumannii in southern Brazil. Moreover, our study suggests that ISAba1/blaOXA-51-like alone is insufficient to confer resistance to carbapenems.

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P71 Antimicrobial resistance patterns for clinical isolates of Bacteroides fragilis group organisms isolated in 2009–2012 at a Korean hospital

International Journal of Antimicrobial Agents ◽

10.1016/s0924-8579(13)70316-9 ◽

2013 ◽

Vol 42 ◽

pp. S64

Author(s):

J.-S. Yim ◽

Y. Lee ◽

M. Kim ◽

Y.H. Seo ◽

W.H. Kim ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Bacteroides Fragilis ◽

Clinical Isolates ◽

Resistance Patterns ◽

Bacteroides Fragilis Group

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Molecular Detection of blaOXA-type Carbapenemase Genes and Antimicrobial Resistance Patterns among Clinical Isolates of Acinetobacter baumannii

Global Medical Genetics ◽

10.1055/s-0041-1740019 ◽

2021 ◽

Author(s):

Maghsoud Kafshnouchi ◽

Marzieh Safari ◽

Amir Khodavirdipour ◽

Abbas Bahador ◽

Seyed Hamid Hashemi ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Acinetobacter Baumannii ◽

Dominant Gene ◽

Diffusion Method ◽

Phylogenetic Tree Analysis ◽

Disk Diffusion Method ◽

Treatment Regimens ◽

Class D ◽

Resistance Patterns ◽

New Treatment

Abstract Acinetobacter baumannii is a bacterium found in most places, especially in clinics and hospitals, and an important agent of nosocomial infections. The presence of class D enzymes such as OXA-type carbapenemases in A. baumannii is proven to have a key function in resistance to carbapenem. The aim of the current study is to determine the blaOXA-type carbapenemase genes and antimicrobial resistance among clinically isolated samples of A. baumannii. We assessed 100 clinically isolated specimens of A. baumannii from patients in intensive care units of educational hospitals of Hamadan, West of Iran. The A. baumannii isolates' susceptibility to antibiotics was performed employing disk diffusion method. Multiplex polymerase chain reaction was used to identify the blaOXA-24-like , blaOXA-23-like , blaOXA-58-like , and blaOXA-51-like genes. The blaOXA-23-like , blaOXA-24-like , and blaOXA-58-like genes' prevalence were found to be 84, 58, and 3%, respectively. The highest coexistence of the genes was for blaOXA-51/23 (84%) followed by blaOXA-51/24-like (58%). The blaOXA-51/23- like pattern of genes is a sort of dominant gene in resistance in A. baumannii from Hamadan hospitals. The highest resistance to piperacillin (83%) and ciprofloxacin (81%) has been observed in positive isolates of blaOXA-23-like . The A. baumannii isolates with blaOXA-58-like genes did not show much resistance to antibiotics. Based on the results of the phylogenetic tree analysis, all isolates have shown a high degree of similarity. This study showed the high frequency of OXA-type carbapenemase genes among A. baumannii isolates from Hamadan hospitals, Iran. Thus, applying an appropriate strategy to limit the spreading of these strains and also performing new treatment regimens are necessary.

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