Biological characteristics of starved Aeromonas hydrophila which contribute to virulence in crucian carp, Carassius cuvieri

1999 ◽  
Vol 154 (2) ◽  
pp. 145-149 ◽  
Author(s):  
M. Habibur Rahman ◽  
Kenji Kawai
Keyword(s):  
Aeromonas Hydrophila ◽  
Crucian Carp ◽  
Biological Characteristics

scholarly journals Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

Marine Drugs ◽  
2019 ◽  
Vol 17 (9) ◽  
pp. 499 ◽  
Author(s):  
Bao Zhang ◽  
Xiyi Zhuang ◽  
Liyun Guo ◽  
Robert J. C. McLean ◽  
Weihua Chu
Keyword(s):  
Virulence Factors ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Crucian Carp ◽  
Homoserine Lactone ◽  
In Vivo Studies ◽  
Acyl Homoserine Lactone ◽  
Signal Molecule ◽  
Promising Alternative ◽  
Feed Supplementation

Quorum quenching (QQ) is a promising alternative infection-control strategy to antibiotics that controls quorum-regulated virulence without killing the pathogens. Aeromonas hydrophila is an opportunistic gram-negative pathogen living in freshwater and marine environments. A. hydrophila possesses an N-acyl homoserine lactone (AHL)-based quorum-sensing (QS) system that regulates virulence, so quorum signal-inactivation (i.e., QQ) may represent a new way to combat A. hydrophila infection. In this study, an AHL lactonase gene, aiiA was cloned from Bacillus sp. strain QSI-1 and expressed in Escherichia coli strain BL21(DE3). The A. hydrophila hexanoyl homoserine lactone (C6-HSL) QS signal molecule was degraded by AiiAQSI-1, which resulted in a decrease of bacterial swimming motility, reduction of extracellular protease and hemolysin virulence factors, and inhibited the biofilm formation of A. hydrophila YJ-1 in a microtiter assay. In cell culture studies, AiiAQSI-1 decreased the ability of A. hydrophila adherence to and internalization by Epithelioma papulosum cyprini (EPC) cells. During in vivo studies, oral administration of AiiAQSI-1 via feed supplementation attenuated A. hydrophila infection in Crucian Carp. Results from this work indicate that feed supplementation with AiiAQSI-1 protein has potential to control A. hydrophila aquaculture disease via QQ.

scholarly journals Draft Genome Sequence of Aeromonas hydrophila Strain BSK-10 (Serotype O97), Isolated from Carassius carassius with Motile Aeromonad Septicemia in China

2017 ◽  
Vol 5 (24) ◽  
Author(s):  
Xiaoyi Pan ◽  
Lingyun Lin ◽  
Yang Xu ◽  
Xuemei Yuan ◽  
Jiayun Yao ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Aeromonas Hydrophila ◽  
Crucian Carp ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Carassius Carassius ◽  
Content Type

ABSTRACT We report here a draft genome sequence of Aeromonas hydrophila strain BSK-10, belonging to serotype O97, isolated from crucian carp (Carassius carassius) with motile aeromonad septicemia in Zhejiang, China. The assembly resulted in 34 scaffolds totaling approximately 4.97 Mb, with an average G+C content of 60.97% and 4,594 predicted coding genes.

Sign in / Sign up

Export Citation Format

Share Document