Are polyamines involved in the synthesis of heat-shock proteins in cell suspension cultures of tobacco and alfalfa in response to high-temperature stress?

Rice plants often encounter high temperature stress, but the associated coping strategies are poorly understood. It is known that a prior shorter exposure to high temperature, called thermo-priming, generally results in better adaptation of the plants to subsequent exposure to high temperature stress. High throughput sequencing of transcript and small RNA libraries of rice seedlings primed with short exposure to high temperature followed by high temperature stress and from plants exposed to high temperature without priming was performed. This identified a number of transcripts and microRNAs (miRs) that are induced or down regulated. Among them osa-miR531b, osa-miR5149, osa-miR168a-5p, osa-miR1846d-5p, osa-miR5077, osa-miR156b-3p, osa-miR167e-3p and their respective targets, coding for heat shock activators and repressors, showed differential expression between primed and non-primed plants. These findings were further validated by qRT-PCR. The results indicate that the miR-regulated heat shock proteins (HSPs)/heat shock transcription factors (HSFs) may serve as important regulatory nodes which are induced during thermo-priming for plant survival and development under high temperatures.

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Proteomic analysis of heat shock proteins in maize (Zea mays L.)

Bulletin of the National Research Centre ◽

10.1186/s42269-019-0251-2 ◽

2019 ◽

Vol 43 (1) ◽

Cited By ~ 1

Author(s):

Mahmoud Hussien Abou-Deif ◽

Mohamed Abdel-Salam Rashed ◽

Kamal Mohamed Khalil ◽

Fatma El-Sayed Mahmoud

Keyword(s):

Heat Treatment ◽

Heat Stress ◽

Heat Shock ◽

Heat Shock Proteins ◽

Proteome Analysis ◽

High Temperature Stress ◽

Heat Treatments ◽

Maize Plants ◽

Adverse Influence ◽

Shock Proteins

Abstract Background Maize is one of the important cereal food crops in the world. High temperature stress causes adverse influence on plant growth. When plants are exposed to high temperatures, they produce heat shock proteins (HSPs), which may impart a generalized role in tolerance to heat stress. Proteome analysis was performed in plant to assess the changes in protein types and their expression levels under abiotic stress. The purpose of the study is to explore which proteins are involved in the response of the maize plant to heat shock treatment. Results We investigated the responses of abundant proteins of maize leaves, in an Egyptian inbred line of maize “K1”, upon heat stress through two-dimensional electrophoresis (2-DE) on samples of maize leaf proteome. 2-DE technique was used to recognize heat-responsive protein spots using Coomassie Brilliant Blue (CBB) and silver staining. In 2-D analysis of proteins from plants treated at 45 °C for 2 h, the results manifested 59 protein spots (4.3%) which were reproducibly detected as new spots where did not present in the control. In 2D for treated plants for 4 h, 104 protein spots (7.7%) were expressed only under heat stress. Quantification of spot intensities derived from heat treatment showed that twenty protein spots revealed clear differences between the control and the two heat treatments. Nine spots appeared with more intensity after heat treatments than the control, while four spots appeared only after heat treatments. Five spots were clearly induced after heat treatment either at 2 h or 4 h and were chosen for more analysis by LC-MSMS. They were identified as ATPase beta subunit, HSP26, HSP16.9, and unknown HSP/Chaperonin. Conclusion The results revealed that the expressive level of the four heat shock proteins that were detected in this study plays important roles to avoid heat stress in maize plants.

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Genes and expression pattern of tobacco mitochondrial small heat shock protein under high-temperature stress

Journal of Plant Biology ◽

10.1007/bf03030450 ◽

2003 ◽

Vol 46 (3) ◽

pp. 204-210 ◽

Cited By ~ 5

Author(s):

Hyun Jo Koo ◽

Xinli Xia ◽

Choo Bong Hong

Keyword(s):

High Temperature ◽

Heat Shock ◽

Heat Shock Protein ◽

Expression Pattern ◽

Temperature Stress ◽

Small Heat Shock Protein ◽

High Temperature Stress

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The Acquisition and Maintenance of Thermotolerance in Australian Wheats

Functional Plant Biology ◽

10.1071/pp9900037 ◽

1990 ◽

Vol 17 (1) ◽

pp. 37 ◽

Cited By ~ 10

Author(s):

C Blumenthal ◽

F Bekes ◽

CW Wrigley ◽

EWR Barlow

Keyword(s):

Heat Treatment ◽

Triticum Aestivum ◽

Protein Synthesis ◽

High Temperature ◽

Heat Shock ◽

High Temperature Stress ◽

Time Interval ◽

Degree Of Protection ◽

Concomitant Reduction ◽

Shock Proteins

The exposure of wheat (Triticum aestivum) coleoptiles to a transient high temperature stress results in the synthesis of a group of proteins known as the heat shock proteins (hsps). The appearance of these proteins is associated with a concomitant reduction in normal protein synthesis and has been correlated with the acquisition of thermotolerance (assessed as growth of coleoptiles). Pretreatment with a sublethal heat shock confers protection to a subsequent heat shock that would otherwise have been lethal. In addition, we find that increasing the time interval between the sublethal heat treatment and the subsequent heat shock from 0 to 72 h reduces the protective effect of the sublethal heat treatment considerably. The five cultivars examined (Sunelg, Sunco, Hartog, Vulcan, Halberd) showed differences in the degree of protection acquired, and in the length of time for which protection was maintained. Hartog was found to be the most thermotolerant, and acquired the greatest degree of protection from exposure to a sublethal heat treatment, but the duration of this acquired protection was shorter than in the remaining cultivars. Sunelg was most susceptible to a heat shock but the duration of acquired protection was the greatest.

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