Study of external low irradiation dose effects on induction of chromosome aberrations in Pisum sativum root tip meristem

2002 ◽  
Vol 517 (1-2) ◽  
pp. 87-99 ◽  
Author(s):  
R Zaka ◽  
C Chenal ◽  
M.T Misset
Keyword(s):  
Pisum Sativum ◽  
Chromosome Aberrations ◽  
Irradiation Dose ◽  
Root Tip ◽  
Dose Effects ◽  
Low Irradiation

Positron Lifetimes and Crystallinity of γ-Irradiated Polypropylenes

2012 ◽  
Vol 733 ◽  
pp. 163-166
Author(s):  
Zhe Chen ◽  
Wei Huang ◽  
Peng Fei Fang ◽  
Wen Yu ◽  
Shao Jie Wang
Keyword(s):  
Differential Scanning Calorimetry ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Positron Lifetime ◽  
Γ Irradiation ◽  
Scanning Calorimetry ◽  
Γ Ray ◽  
Positron Lifetimes ◽  
Dsc Method ◽  
Low Irradiation

The microstructure of the polypropylenes (PP) irradiated by gamma ray was studied by positron lifetime technique and differential scanning calorimetry (DSC). Lifetime measurement shows o-Ps intensity decreases with increasing γ irradiation dose. The crystallinity of irradiated samples was detected by DSC method. The correlation between o-Ps intensity and crystallinity indicates γ-ray can induce higher crystallinity in the polypropylene at low irradiation dose.

Observations of the Golgi Apparatus in Pisum Primary Roots

1968 ◽  
Vol 26 ◽  
pp. 14-15
Author(s):  
Gordon C. Spink
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Pisum Sativum ◽  
Golgi Apparatus ◽  
Common Garden ◽  
Root Tip ◽  
Garden Pea ◽  
Wall Area ◽  
Primary Roots ◽  
Columella Cells

It is known that the product of the Golgi apparatus vesicles is deposited at and localized in the cell wall. This is accomplished by the formation of the hypertrophied dictyosomes and the subsequent movement of these vesicles to the plasma membrane (Fig. 1). After fusion with the plasma membrane, the secreted material is released into the cell wall area and, in some plants under appropriate conditions, moves outward through the cell wall and appears as a droplet on the root tip.In primary roots of Pisum sativum, var. Alaska (common garden pea) the Golgi apparatus vesicle product accumulates between the plasma membrane and the cell wall, particularly in those cells at the extreme tip of the root. These cells are formed at the acropetal end of the columella cells.

The arrangement of Pisum chromosomes in interphase

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 717-722 ◽  
Author(s):  
Gisela Wolff ◽  
Hans-Dietrich Quednau
Keyword(s):  
Pisum Sativum ◽  
Root Tip ◽  
Homologous Chromosomes ◽  
Multiple Tests ◽  
Nucleolar Organizing Region ◽  
Tests Of Significance ◽  
Chromosome Position ◽  
Somatic Pairing ◽  
Tip Cells ◽  
Root Tip Cells

Root tip cells of Pisum sativum were analyzed for the position of two chromosomes (4 and 7) that carry the nucleolar organizing region. Because of a characteristic heterochromatic pattern of these chromosomes, they can be identified in interphase. The positions of these regions were measured in relation to the center of the nucleus and to one another. The values were statistically evaluated by means of multiple tests of significance. The results discussed show clearly that in P. sativum cells the distribution of the nucleolar organizing chromosomes is not random and that the chromosomes analysed occupy special sites. There was no evidence that homologous chromosomes are more adjacent than heterologous ones, nor that morphologically similar chromosomes are neighbours. The present investigations have also shown that the position of the chromosomes is altered if the structure of the chromosomes is changed by reciprocal translocations.Key words: chromosome position, somatic pairing, translocation, heterochromatin, C-banding, multiple tests of significance.

DISTRIBUTION OF HETEROCHROMATIN IN THE CHROMOSOMES OF HAPLOPAPPUS GRACILIS

1968 ◽  
Vol 10 (2) ◽  
pp. 433-443 ◽  
Author(s):  
Ira H. Ames ◽  
Jyotirmay Mitra
Keyword(s):  
Aqueous Solution ◽  
Chromosome Aberrations ◽  
Maleic Hydrazide ◽  
Cold Treatment ◽  
The Other ◽  
Root Tip ◽  
Metaphase Chromosomes ◽  
Haplopappus Gracilis ◽  
Tip Cells ◽  
Root Tip Cells

Several approaches were employed to study the distribution of heterochromatin in root tip chromosomes of Haplopappus gracilis. Cold treatment and pretreatment in an aqueous solution of 8-hydroxyquinoline revealed achromatic gaps in metaphase chromosomes. Cold treatment also permitted the demonstration of positive heteropycnosis in prophase chromosomes. Further support for the identification of heterochromatic segments was provided by a study of the localization of chromosome aberrations induced by maleic hydrazide and an analysis of the pattern of DNA synthesis in chromosomes of root tip cells. Seven of the ten regions that were preferentially broken by maleic hydrazide also reacted differentially to cold treatment or to pretreatment with 8-hydroxyquinoline. A good correlation was found between regions that completed DNA replication late in the DNA-synnhetic period and segments that were shown to be heterochromatic by the other techniques.

scholarly journals Impact of Bisphenol A on seed germination, radicle length and cytogenetic alterations in Pisum sativum L

Caryologia ◽  
2021 ◽  
Author(s):  
Sazada Siddiqui ◽  
Saad Abdurahamn Muhammad Al Amri ◽  
Huda Ahmed Al Ghamdy ◽  
Wadha Saad Saeed Alqahtani ◽  
Sarah Mohammed Alquyr ◽  
...  
Keyword(s):  
Seed Germination ◽  
Pisum Sativum ◽  
Bisphenol A ◽  
Harmful Effect ◽  
Root Tip ◽  
Chromosomal Anomalies ◽  
Pisum Sativum L ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Radicle Length

Bisphenol A (BPA) is a global transpiring pollutant and an endocrine disruptor present in the environment which has a substantial harmful effect on plants. In the present study, its effects on seed germination, radicle length and cytogenetic alterations were investigated in Pisum sativum L root tip cells. Pisum sativum L seeds were germinated after treating with various concentrations of BPA (2 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, 20 mg/L and 25 mg/L) at 24±1°C for 72 hours and the cytogenetic variations were assessed. The investigation showed that BPA reduced the percentage of seed germination, mitotic index, radicle length (at higher concentrations) and instigated a rise in chromosomal anomalies in a dose-related manner. In total, there is an enhanced occurrence of c-mitosis, stickiness, bridges, fragments and laggards in the BPA treated root tip cells of Pisum sativum L seeds.

Altered growth and root tip morphology in Pisum sativum L. in response to altered expression of a gene expressed in border cells

2013 ◽  
Vol 377 (1-2) ◽  
pp. 179-187 ◽  
Author(s):  
Fushi Wen ◽  
Lindy A. Brigham ◽  
Gilberto Curlango-Rivera ◽  
Zhongguo Xiong ◽  
Martha C. Hawes
Keyword(s):  
Pisum Sativum ◽  
Root Tip ◽  
Border Cells ◽  
Altered Expression ◽  
Pisum Sativum L

Cytotoxic and genotoxic effects of Lavandula stoechas aqueous extracts

Biologia ◽  
2007 ◽  
Vol 62 (3) ◽  
Author(s):  
Tülay Çelik ◽  
Özlem Aslantürk
Keyword(s):  
Medicinal Plant ◽  
Mitotic Index ◽  
Chromosome Aberrations ◽  
Spring Water ◽  
Root Tip ◽  
Aqueous Extracts ◽  
Genotoxic Effects ◽  
Lavandula Stoechas ◽  
The World ◽  
Water Ph

AbstractLavandula genus is an important member of Labiatae (Lamiaceae) family. People use commonly Lavandula stoechas as a medicinal plant for various diseases around the world and also in Turkey. The aim of this study was to investigate cytotoxic and genotoxic effects of aqueous extracts (40, 80 and 120 g/L) from L. stoechas flowers on Allium cepa root tip meristem cells. For this purpose, A. cepa onion bulbs were treated with the above-mentioned L. stoechas flower extracts for 72 h. Spring water (pH 7.3) was used as a control. The result of this study sowed that aqueous extracts reduced mitotic index, but induced chromosome aberrations and mitotic aberrations in comparison with control, significantly (p < 0.05). Aqueous extracts induced breaks, stickiness, pole deviations and micronuclei. Furthermore, these effects were related to extract concentrations. These results showed that L. stoechas aqueous extracts have cytotoxic and genotoxic effects.

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