A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae

1997 ◽  
Vol 9 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Maureen G. Friedman ◽  
Shlomo Ilan ◽  
Simona Kahane ◽  
Nani Kosashvili ◽  
Yona Bir ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydia Pneumoniae ◽  
Igg Antibodies

scholarly journals Serological Investigation of Chlamydia trachomatis Heat Shock Protein 10

1999 ◽  
Vol 67 (10) ◽  
pp. 5243-5246 ◽  
Author(s):  
Fotini Betsou ◽  
Jean Marie Sueur ◽  
Jeanne Orfila
Keyword(s):  
Heat Shock ◽  
Chlamydia Trachomatis ◽  
Heat Shock Protein ◽  
Genital Tract ◽  
Chlamydia Pneumoniae ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Genital Tract Infection ◽  
Humoral Immune ◽  
Tract Infections

ABSTRACT The humoral immune response to Chlamydia trachomatis10-kDa heat shock protein (Chsp10) in populations of Russian and French origin was studied by using a recombinant Chsp10 enzyme-linked immunosorbent assay. A physiological but not a serological correlation of Chsp10 exposure with Chsp60 exposure was observed in the Russian population. In the French population studied, there was a significant association between detection of anti-r-Chsp10 immunoglobulin G (IgG) antibodies and chronic genital tract infections. Chsp10 residues 50 to 67 were found to contain an immunodominant although not universal B epitope. Cross-reactions with Chlamydia pneumoniae orEscherichia coli GroES protein are limited but may occur. Our study suggests that detection of anti-Chsp10 IgG antibodies is associated with chronicity of C. trachomatis genital tract infection and does not parallel that of anti-Chsp60 IgG antibodies.

Sensitivity and specificity of three new commercially available Chlamydia trachomatis tests

2002 ◽  
Vol 13 (1_suppl) ◽  
pp. 23-25 ◽  
Author(s):  
R P Verkooyen ◽  
M F Peeters ◽  
J H Van Rijsoort-Vos ◽  
W I Van Der Meijden ◽  
J W Mouton
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydia Pneumoniae ◽  
Strongly Correlated ◽  
Igg Antibodies ◽  
Active Infection ◽  
Serum Samples ◽  
Iga Antibodies ◽  
Donor Population ◽  
Polymerase Chain ◽  
Species Specific

In order to determine the value of new Chlamydia trachomatis (Ct) specific tests for routine serological diagnosis of Ct infections, we evaluated several commercially available assays (C. trachomatis enzyme immunoassay (EIA), Labsystems (CtL); SeroCT, Savyon (CtS); pELISA, Medac (CtMp)) in various study populations. The prevalence of C. trachomatis-specific IgA antibodies in a blood donor population (n=443) as determined by the peptide based tests CtL, the CtS and the CtMp was 5%, while for IgG antibodies this was 6% (CtL and CtS) and 12% (CtMp) respectively. Prevalence was negatively correlated with age, concording with C. trachomatis specificity. None of the three tests showed significant titre rises in serum samples taken from patients with a proven infection of Chlamydia pneumoniae (n=22), indicating species-specificity for all three tests. In patients with a polymerase chain reaction proven (n = 324) Ct infection, 75%, 70% and 68% were positive for IgG and 45%, 38% and 47% positive for IgA as determined by the CtMp, CtL and CtS respectively. We conclude that the new synthetic peptide-based EIA tests are able to detect species-specific Ct antibodies, which are strongly correlated to (active) infection.

scholarly journals Prevalence of Chlamydia pneumoniae seropositivity in early pregnancy and its association with preeclampsia among primigravidae in Indian population

2019 ◽  
Vol 8 (10) ◽  
pp. 3964
Author(s):  
Lavi Sindhu ◽  
Bindoo Yadav ◽  
Aruna Batra
Keyword(s):  
Early Pregnancy ◽  
Indian Population ◽  
Chlamydia Pneumoniae ◽  
Igg Antibodies ◽  
Interventional Study ◽  
Low Birth Weight Babies ◽  
Elisa Technique ◽  
Effect Of Treatment ◽  
Inflammatory Changes ◽  
Oral Azithromycin

Background: Preeclampsia (PE) is a multifactorial disease that might be caused by a concurrent or preceding inflammatory stimulus. Inflammatory changes similar to those reported in chronic Chlamydia pneumoniae infection are seen in PE. It is suggested that persistent or chronic Chlamydia pneumoniae infection might have a role in the pathogenesis of PE and antichlamydial treatment in early pregnancy may prevent reactivation of infection and hence the development of preeclampsia.Methods: This randomized interventional study was conducted to determine the prevalence of C.pneumoniae IgG seropositivity in early pregnancy, its association with PE and the effect of treatment with oral azithromycin. A total of 330 primigravidae included in the study were followed up till delivery. C.pneumoniae IgG antibodies measured by ELISA technique at 14-20 weeks of gestation revealed seropositivity in 32.4%. These women were at higher risk of developing severe PE (odds’ ratio 3.2) as compared to the C. pneumoniae seronegative cases.Results: Treatment with oral azithromycin resulted in reduction in the occurrence of PE amongst the C.pneumoniae seropositive cases; as well as significant reduction in the incidence of low birth weight babies in the C.pneumonie seropositive group (p<0.001, ARR= 0.204).Conclusions: Pregnant women who were C.pneumonia IgG seropositive are at higher risk of developing severe preeclmapsia as compared to the C.pneumoniae seronegative cases. This association needs to be further evaluated.

scholarly journals Testing patients with uveal melanoma for herpesvirus infections

2016 ◽  
Vol 61 (6) ◽  
pp. 284-287 ◽  
Author(s):  
S. V. Saakyan ◽  
E. B. Myakoshina ◽  
G. I. Krichevskaya ◽  
O. S. Slepova ◽  
O. G. Panteleeva ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Uveal Melanoma ◽  
Vitreous Body ◽  
Human Herpesvirus ◽  
Infectious Agents ◽  
Igg Antibodies ◽  
Tissue Samples ◽  
Human Herpes Virus 8 ◽  
Barr Virus ◽  
Simplex Virus

Results of comprehensive ELISA tests of blood serum for the presence of IgM-, IgA-, and IgG-antibodies to herpes simplex virus types 1 and 2, cytomegalovirus, Epstein-Barr virus, human herpesvirus 6, human herpes virus 8 type, Chlamydia trachomatis in 38 patients with uveal melanoma are presented. The polymerase chain reaction was used to detect DNA of these pathogens in tumor biopsies, vitreous body of 10 enucleated eyes, as well as in plasma IgG-antibodies to HHV 6 were revealed in 50% of patients; IgG-antibodies to HHV 8, in 5.3% of patients. Among the 16 patients with uveal melanoma at advanced stages, 6 patients had antibodies indicative of EBV reactivation (1.2-3.3). Chlamydia trachomatis genome was detected in both biopsies; in one of them, in conjunction with EBV and CMV DNA . Tissue samples from the identified infectious agents were related only to the spindle-cell histologic type AB of uveal melanoma. In plasma, genomes of pathogens were not determined. The results indicate the presence of infectious agents in patients with uveal melanoma and require further study of the pathogenetic role of infections in the pathogenesis of uveal melanoma.

scholarly journals Chlamydia pneumoniae Uses the Mannose 6-Phosphate/Insulin-Like Growth Factor 2 Receptor for Infection of Endothelial Cells

2005 ◽  
Vol 73 (8) ◽  
pp. 4620-4625 ◽  
Author(s):  
Mirja Puolakkainen ◽  
Cho-Chou Kuo ◽  
Lee Ann Campbell
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Outer Membrane Protein ◽  
Chlamydia Pneumoniae ◽  
Insulin Like Growth Factor ◽  
Inhibition Assay ◽  
High Mannose ◽  
Mannose 6 Phosphate

ABSTRACT Several mechanisms for attachment and entry of Chlamydia have been proposed. We previously determined that the major outer membrane protein of Chlamydia trachomatis is glycosylated with a high-mannose oligosaccharide, and a similar structure inhibited the attachment and infectivity of C. trachomatis in epithelial cells. Because insulin-like growth factor 2 (IGF2) was shown to enhance the infectivity of Chlamydia pneumoniae but not C. trachomatis in endothelial cells, a hapten inhibition assay was used to analyze whether the mannose 6-phosphate (M6P)/IGF2 receptor that also binds M6P could be involved in infection of endothelial cells (HMEC-1) by Chlamydia. M6P and mannose 6-phosphate-poly[N-(2-hydroxyethyl)-acrylamide] (M6P-PAA) inhibited the infectivity of C. pneumoniae AR-39, but not C. trachomatis serovar UW5 or L2, while mannan inhibited the growth of C. trachomatis, but not C. pneumoniae. Using metabolically labeled organisms incubated with cells at 4°C (organisms attach but do not enter) or at 37°C (organisms attach and are internalized), M6P-PAA was shown to inhibit attachment and internalization of C. pneumoniae in endothelial cells but did not inhibit attachment or internalization of C. trachomatis serovar E or L2. These findings indicate that C. pneumoniae can utilize the M6P/IGF2 receptor and that the use of this receptor for attachment and entry differs between C. pneumoniae and C. trachomatis.

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