P.144 The effects of aqueous extract of garlic (allium sativum I.) On laryngeal cancer cells (hep-2) and 1929 cells in vitro

Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma.In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells.In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted.

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MicroRNA‑195‑5p suppresses the proliferation, migration, invasion and epithelial‑mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

Experimental and Therapeutic Medicine ◽

10.3892/etm.2021.10512 ◽

2021 ◽

Vol 22 (4) ◽

Author(s):

Min Zhou ◽

Yu Wang ◽

Changming Zhang ◽

Meihao Qi ◽

Min Yao ◽

...

Keyword(s):

Cancer Cells ◽

Laryngeal Cancer ◽

Epithelial Mesenchymal Transition ◽

Mesenchymal Transition

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Effects of ATP-binding cassette transporter G2 in extracellular vesicles on drug resistance of laryngeal cancer cells in in vivo and in vitro

Oncology Letters ◽

10.3892/ol.2021.12625 ◽

2021 ◽

Vol 21 (5) ◽

Author(s):

Yan Zhao ◽

Yuetong Chen ◽

Jing Wang ◽

Liang Liu

Keyword(s):

Drug Resistance ◽

Cancer Cells ◽

Extracellular Vesicles ◽

Laryngeal Cancer ◽

Atp Binding ◽

Atp Binding Cassette Transporter ◽

Atp Binding Cassette

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Enhanced Anticancer Property of Ad-MSC-TRAIL on Breast Cancer Cells When Combined with the Aqueous Extract of Berberis vulgaris

Jentashapir Journal of Cellular and Molecular Biology ◽

10.5812/jjcmb.107951 ◽

2020 ◽

Vol 11 (2) ◽

Author(s):

Mahdieh Khorashadizadeh ◽

Nahid Khazaei Moghadam ◽

Nasrin Zandi Dasht-e-Bayaz ◽

Mohsen Khorashadizadeh

Keyword(s):

Breast Cancer ◽

Combination Therapy ◽

Cancer Cells ◽

Tumor Cells ◽

Aqueous Extract ◽

Cancer Cell Line ◽

Expression Vectors ◽

Berberis Vulgaris ◽

Mcf 7

Background: Although there are improvements in breast cancer diagnosis and treatment methods, some breast tumor cells still are resistant to current therapies. Thus, there are attempts all over the world to find an effective way with more toxicity to tumor cells and less to normal cells. In recent years, mesenchymal stem cells (MSCs), due to their native tumor homing property, have been introduced as expression vectors for anticancer proteins, such as TNF-related apoptosis-inducing ligand (TRAIL). However, most tumor cells are resistant to TRAIL or show low sensitivity to it. Thus, it is necessary to find a way to increase the sensitivity of cancer cells and decrease their resistance. One of these ways is combination therapy with herbal drugs. Objectives: This study aimed to investigate the combination of sub-toxic doses of aqueous extract of Berberis vulgaris (AEBV) and MSC-TRAIL on MCF-7 cells as a human breast cancer cell line. Methods: Experiments were set based on in vitro cell culture. Combination therapy was carried out in transwell co-culture plates. The cell viability was determined by the MTT assay. The cell cycle was measured using the Propidium Iodide (PI) staining flow cytometry method. All experiments were performed in triplicate. The data were analyzed by One-way ANOVA test using SPSS software. Results: MCF-7 cells were relatively resistant to MSC-TRAIL and AEBV alone, while a sub-toxic concentration of AEBV (0.5 mg/mL) combined with MSC-TRAIL significantly increased death in MCF-7 cells, showing a synergistic effect.

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