Assessing Plant Antioxidants by Cellular Antioxidant Activity Assay Based on Microfluidic Cell Chip with Arrayed Microchannels

AbstractFurther chemical investigation of the fruit of the mangrove Avicennia marina led to the isolation of a new caffeic acid derivative, maricaffeolylide A (1), and a new megastigmane derivative, maricyclohexene A (2). The structures of the isolates were elucidated on the basis of extensive spectroscopic analysis. The antioxidant activity of 1 and 2 was also evaluated using a cellular antioxidant activity assay, and catechol 1 showed antioxidant activity with an EC50 value of 24 ± 0.3 μm.

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Structural Characterization andIn VitroAntioxidant Activity of Kojic Dipalmitate Loaded W/O/W Multiple Emulsions Intended for Skin Disorders

BioMed Research International ◽

10.1155/2015/304591 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 13

Author(s):

Maíra Lima Gonçalez ◽

Diana Gleide Marcussi ◽

Giovana Maria Fioramonti Calixto ◽

Marcos Antonio Corrêa ◽

Marlus Chorilli

Keyword(s):

Antioxidant Activity ◽

Safety Profile ◽

Flow Behavior ◽

Microscopic Analysis ◽

Skin Disorders ◽

Activity Assay ◽

Multiple Emulsions ◽

Lipophilic Antioxidant ◽

Newtonian Behavior

Multiple emulsions (MEs) are intensively being studied for drug delivery due to their ability to load and increase the bioavailability of active lipophilic antioxidant, such as kojic dipalmitate (KDP). The aim of this study was to structurally characterize developed MEs by determining the average droplet size (Dnm) and zeta potential (ZP), performing macroscopic and microscopic analysis and analyzing their rheological behavior andin vitrobioadhesion. Furthermore, thein vitrosafety profile and antioxidant activity of KDP-loaded MEs were evaluated. The developed MEs showed a Dnm of approximately 1 micrometer and a ZP of −13 mV, and no change was observed in Dnm or ZP of the system with the addition of KDP. KDP-unloaded MEs exhibited ‘‘shear thinning’’ flow behavior whereas KDP-loaded MEs exhibited Newtonian behavior, which are both characteristic of antithixotropic materials. MEs have bioadhesion properties that were not influenced by the incorporation of KDP. The results showed that the incorporation of KDP into MEs improved the safety profile of the drug. Thein vitroantioxidant activity assay suggested that MEs presented a higher capacity for maintaining the antioxidant activity of KDP. ME-based systems may be a promising platform for the topical application of KDP in the treatment of skin disorders.

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PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY GLYCYRRHIZA PALLIDIFLORA HERB (GLYCYRRHIZA PALLIDIFLORA MAXIM.)

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2021-23-5-42-46 ◽

2021 ◽

pp. 42-46

Author(s):

Maltseva E.M. ◽

Egorova I.N. ◽

Pinchuk L.G.

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Russian Far East ◽

Far East ◽

Biologically Active ◽

Perennial Herb ◽

Total Phenolic ◽

Plant Antioxidants ◽

Phytochemical Studies ◽

First Time

Pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.) is the pea family (Fabaceae) perennial herb. It is a Russian Far East endemic. Pale-flowered licorice refers to the false (not sweet) licorice section (Pseudoglycyrrhiza Krug.), which do not accumulate glycyrrhizin derivatives. The G. pallidiflora successful introduction has been carried out over the past 5 years in the “Apothecary garden” territory, FRC UUH SB RAS, Kemerovo. Phytochemical studies screening biologically active compounds main classes of the plant aerial part confirmed the presence of a complex of phenolic compounds. The content of catechin derivatives and condensed-type tannins - proanthocyanidins (PAC) data in G. pallidiflora herb were obtained for the first time. In different years of cultivation, licorice herb accumulates phenolic compounds - up to 2.83 ± 0.22% in terms of gallic acid, flavonoids - up to 2.44 ± 0.03% in terms of rutin and PAC in terms of cyanidine chloride - up to 2.61 ± 0.11%. It was found that the maximum content of the phenolic compounds and PAC sum was observed in the herb for 4 years development. The greatest number of flavonoids accumulates in the herb harvested in the 3rd year of cultivation. A positive significant linear relation was found between antioxidant activity (AOA) and the total phenolic compounds and PAC content (r≥0.98). Considering that BAC with IC values ≤ 50 μg / ml in the DPPH assay refer to active antioxidant’s licorice herb can be classified as a plant with high antioxidant potential. It was found that the iron chelating activity of the G. pallidiflora herb is in direct dependence (r≥0.94) on the content of flavonoids. The obtained results demonstrate the importance of further study of this plant as a source of BAC, including plant antioxidants.

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Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp

Food Chemistry ◽

10.1016/j.foodchem.2014.02.134 ◽

2014 ◽

Vol 158 ◽

pp. 385-391 ◽

Cited By ~ 39

Author(s):

Dongxiao Su ◽

Huihui Ti ◽

Ruifen Zhang ◽

Mingwei Zhang ◽

Zhengchen Wei ◽

...

Keyword(s):

Antioxidant Activity ◽

Structural Elucidation ◽

Cellular Antioxidant Activity ◽

Activity Evaluation

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Betalains in Edible Fruits of Three Cactaceae Taxa—Epiphyllum, Hylocereus, and Opuntia—Their LC-MS/MS and FTIR Identification and Biological Activities Evaluation

Plants ◽

10.3390/plants10122669 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2669

Author(s):

Michaela Barkociová ◽

Jaroslav Tóth ◽

Katarzyna Sutor ◽

Natalia Drobnicka ◽

Slawomir Wybraniec ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Food Industry ◽

Biological Activities ◽

Ornamental Plants ◽

Reducing Power ◽

Activity Assay ◽

Natural Pigment ◽

E Coli ◽

Ft Ir

Epiphyllum, Hylocereus, and Opuntia plants belong to the Cactaceae family. They are mostly known as ornamental plants but also for their edible fruits, which can potentially be sources of betalains, such as betanin, a natural pigment used in the food industry, e.g., under the European label code E 162. The aim of this work was the identification of betalains (using LC-MS/MS), evaluation of total betalain content (spectrophotometrically), analysis of functional groups (using FT-IR), evaluation of antioxidant activity (using DPPH, ABTS, FRAP, DCFH-DA, and reducing power methods) and evaluation of antimicrobial activity (S. aureus, E. coli, and C. albicans) in fruits of Epiphyllum, Hylocereus, and Opuntia taxa. A total of 20 betalains were identified in the studied Cactaceae fruits. The Epiphyllum pink hybrid had the highest values of total betalains amongst all samples. The highest antioxidant activity was observed in the Epiphyllum pink hybrid, in Opuntia zacuapanensis and O. humifusa fruits. The antimicrobial activity assay showed that cacti fruits were not able to effectively inhibit the growth of E. coli, S. aureus, or C. albicans. Our results prove that these fruits are good sources of natural pigments—betalains. They do not contain toxic compounds in significant amounts and they exhibit antioxidant activity.

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