Changes in articular cartilage and subchondral bone histomorphometry in osteoarthritic knee joints in humans

Bone ◽  
2003 ◽  
Vol 32 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Dragica Bobinac ◽  
Josip Spanjol ◽  
Sanja Zoricic ◽  
Ivana Maric
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Bone Histomorphometry ◽  
Knee Joints ◽  
Osteoarthritic Knee

Distribution of vitamin K2 in subchondral bone in osteoarthritic knee joints

2012 ◽  
Vol 21 (8) ◽  
pp. 1813-1818 ◽  
Author(s):  
Yoshinori Ishii ◽  
Hideo Noguchi ◽  
Mitsuhiro Takeda ◽  
Junko Sato ◽  
Noriaki Yamamoto ◽  
...  
Keyword(s):  
Subchondral Bone ◽  
Vitamin K2 ◽  
Knee Joints ◽  
Osteoarthritic Knee

scholarly journals Validity of histopathological grading of articular cartilage from osteoarthritic knee joints

1999 ◽  
Vol 58 (4) ◽  
pp. 208-213 ◽  
Author(s):  
K. Ostergaard ◽  
C. B Andersen ◽  
J. Petersen ◽  
K. Bendtzen ◽  
D. M Salter
Keyword(s):  
Articular Cartilage ◽  
Knee Joints ◽  
Osteoarthritic Knee ◽  
Histopathological Grading

scholarly journals Effect of intraarticular injection of chondroitin sulfate into synovial joint elements in an experiment

2020 ◽  
pp. 84-89
Author(s):  
V. I. Nikolaev ◽  
D. A. Zinovkin ◽  
A. A. Tretyakov
Keyword(s):  
Articular Cartilage ◽  
Chondroitin Sulfate ◽  
Subchondral Bone ◽  
Growth Zone ◽  
Epiphyseal Cartilage ◽  
Knee Joints ◽  
Joint Control ◽  
Synovial Joint ◽  
Cell Content ◽  
And Control

Objective: to study morphological and morphometric changes in the epiphysis bone in a rat during the intra-articular injection of chondroitin sulfate (CS). Mаteriаl аnd methods. The object of the study was the knee joints of 36 Wistar rats. CS injections at a dose of 0.05 ml were performed once a week into one of the knee joints (experimental joint), and isotonic NaCl solution at the same volume was injected into the opposite joint (control joint). The animals in the number of 12 units were withdrawn from the experiment on the 7th, 14th, and 21st days, which corresponded to one week after one-, two-and three-fold intra-articular injections of HC and 0.9 % NaCl. The isolated knee joints were placed in a decalcifying liquid, then were fixed in 10% neutral buffered formalin. 4 micron histological sections were stained with hematoxylin and eosin. The morphometric analysis assessed the thickness of hyaline articular cartilage, the thickness of the epiphysis growth zone cartilage, and the cell content of the subchondral bone. Results. The study of the thickness of the articular cartilage, the growth zone of epiphyseal cartilage and the cellular composition of the subchondral bone has showed a statistically significant dynamic increase in these indicators after the 2nd and 3rd intra-articular injections of CS. The assessment of the thickness of the articular cartilage on the 21st day found some statistically significant differences between the experimental and control groups (p < 0.0001), the thickness of the epiphyseal cartilage had increased significantly by that time (p < 0.0001), and the cell content of bone marrow showed statistically significant differences (p = 0.002). Conclusion. The obtained data testify to a pronounced regenerative effect of CS, injected intra-articularly into articular cartilage.

Relationship between subchondral bone microstructure and articular cartilage in the osteoarthritic knee using 3T MRI

2018 ◽  
Vol 48 (3) ◽  
pp. 669-679 ◽  
Author(s):  
Chenglei Liu ◽  
Chang Liu ◽  
Liping Si ◽  
Hao Shen ◽  
Qian Wang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Bone Microstructure ◽  
3T Mri ◽  
Osteoarthritic Knee

scholarly journals The alteration of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) in the knee joints of osteoarthritis mice

2019 ◽  
Author(s):  
Luling Wang ◽  
Demao Zhang ◽  
Jianxun Sun ◽  
Yujia Cui ◽  
Linyi Cai ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Cruciate Ligament ◽  
Degenerative Change ◽  
Knee Joints ◽  
Cartilage Growth ◽  
Anterior Cruciate ◽  
A Disintegrin And Metalloproteinase ◽  
Cartilage Growth Plate ◽  
Gene Expression Levels

Abstract Background: To clarify the expression and distribution of ADAMTS1, ADAMTS2 and ADAMTS5 in knee joints of osteoarthritis (OA) mice. Methods: OA was established via anterior cruciate ligament transection (ACLT) on the knee joints of C57BL/6J mice. The morphology change of OA was analyzed by Micro-CT. Histologic analysis was used to evaluate symptomatic change in articular cartilage and subchondral bone. Quantitative real-time PCR (qPCR) was used to analyze mRNA expressions of ADAMTS family in bone-related tissues and cells. Immunofluorescence staining was used to analyze the expressions and distributions of ADAMTS1, ADAMTS2, and ADAMTS5, as well as the condition of inflammation of OA.Results: Cartilage deterioration, significant reduction of collagen and proteoglycan components in the cartilage matrix happened in ACLT-induced OA mice, along with increased inflammatory response and osteoclast activity. Among ADAMTS, the gene expression levels of ADAMTS1, ADAMTS2 and ADAMTS5 were ranked top 5 in cartilage/chondrocytes, osteogenic tissue/osteoblasts and cortical bone/osteocytes. After ACLT surgery, the expressions of ADAMTS1, ADAMTS2 and ADAMTS5 all increased in articular cartilage, growth plate and subchondral bone of knee joints. Conclusion: The enhanced expressions of ADAMTS1, ADAMTS2 and ADAMTS5 after ACLT surgery provide a further understanding in degenerative change of OA.

Calcium-Suppressed Technique in Dual-Layer Detector Computed Tomography to Evaluate Knee Articular Cartilage

2020 ◽  
Vol 16 ◽  
Author(s):  
Qinglin Meng ◽  
Mengqi Liu ◽  
Weiwei Deng ◽  
Ke Chen ◽  
Botao Wang ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Marrow ◽  
Articular Cartilage ◽  
Bone Marrow Edema ◽  
Knee Joints ◽  
Infrapatellar Fat Pad ◽  
Proton Density ◽  
Knee Cartilage ◽  
Significant Difference ◽  
Marrow Edema

Background: Calcium-suppressed (CaSupp) technique involving spectral-based images has been used to observe bone marrow edema by removing calcium components from the image. Objective: This study aimed to evaluate the knee articular cartilage using the CaSupp technique in dual-layer detector computed tomography (DLCT). Methods: Twenty-eight healthy participants and two patients with osteoarthritis were enrolled, who underwent DLCT and magnetic resonance imaging (MRI) examination. CaSupp images were reconstructed from spectral-based images using a calcium suppression algorithm and were overlaid conventional CT images for visual evaluation. The morphology of the knee cartilage was evaluated, and the thickness of the articular cartilage was measured on sagittal proton density– weighted and CaSupp images in the patellofemoral compartment. Results: No abnormal signal or density, cartilage defect, and subjacent bone ulceration were observed in the lateral and medial femorotibial compartments and the patellofemoral compartment on MRI images and CaSupp images for the 48 normal knee joints. CaSupp images could clearly identify cartilage thinning, defect, subjacent bone marrow edema, and edema of the infrapatellar fat pad in the same way as MRI images in the three knee joints with osteoarthritis. A significant difference was found in the mean thickness of the patellar cartilage between MRI images and CaSupp images, while the femoral cartilage presented no significant difference in thickness between MRI images and CaSupp images over all 48 knee joints. Conclusion: The present study demonstrated that CaSupp images could effectively be used to perform the visual and quantitative assessment of knee cartilage.

scholarly journals Genetic Deletion of Interleukin-15 Is Not Associated with Major Structural Changes Following Experimental Post-Traumatic Knee Osteoarthritis in Rats

2021 ◽  
Vol 11 (15) ◽  
pp. 7118
Author(s):  
Ermina Hadzic ◽  
Garth Blackler ◽  
Holly Dupuis ◽  
Stephen James Renaud ◽  
Christopher Thomas Appleton ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Structural Changes ◽  
Cartilage Damage ◽  
Wild Type ◽  
Significant Difference ◽  
Post Traumatic ◽  
Interleukin 15 ◽  
Joint Trauma ◽  
Surgically Induced

Post-traumatic osteoarthritis (PTOA) is a degenerative joint disease, leading to articular cartilage breakdown, osteophyte formation, and synovitis, caused by an initial joint trauma. Pro-inflammatory cytokines increase catabolic activity and may perpetuate inflammation following joint trauma. Interleukin-15 (IL-15), a pro-inflammatory cytokine, is increased in OA patients, although its roles in PTOA pathophysiology are not well characterized. Here, we utilized Il15 deficient rats to examine the role of IL-15 in PTOA pathogenesis in an injury-induced model. OA was surgically induced in Il15 deficient Holtzman Sprague-Dawley rats and control wild-type rats to compare PTOA progression. Semi-quantitative scoring of the articular cartilage, subchondral bone, osteophyte size, and synovium was performed by two blinded observers. There was no significant difference between Il15 deficient rats and wild-type rats following PTOA-induction across articular cartilage damage, subchondral bone damage, and osteophyte scoring. Similarly, synovitis scoring across six parameters found no significant difference between genetic variants. Overall, IL-15 does not appear to play a key role in the development of structural changes in this surgically-induced rat model of PTOA.

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