scholarly journals Bone mineral content is positively correlated to n-3 fatty acids in the femur of growing rats

2010 ◽  
Vol 104 (5) ◽  
pp. 674-685 ◽  
Author(s):  
Yong Li ◽  
Mark F. Seifert ◽  
Sun-Young Lim ◽  
Norman Salem ◽  
Bruce A. Watkins
Keyword(s):  
Fatty Acid ◽  
Bone Mineral ◽  
Mineral Content ◽  
Bone Growth ◽  
Lumbar Vertebrae ◽  
Docosapentaenoic Acid ◽  
Acid Group ◽  
Fatty Acid Analysis ◽  
Long Bone ◽  
Mineral Density

The present study was conducted to determine whether provision of preformed dietary docosapentaenoic acid (DPAn-6) can replace DHA for normal long bone growth as assessed by dual-energy X-ray absorptiometry for mineral content (BMC). A newly modified artificial rearing method was employed to generate n-3 fatty acid-deficient rats. Except the dam-reared (DR; 3·1 % α-linolenic acid) group, newborn pups were separated from their mothers at age 2 d and given artificial rat milk containing linoleic acid (LA), or LA supplemented with 1 % DHA (22 : 6n-3; DHA), 1 % DPAn-6 (DPA), or 1 % DHA plus 0·4 % DPAn-6 (DHA/DPA). The rats were later weaned onto similar pelleted diets. At adulthood, the rats were euthanised and bones (femur, tibia, and lumbar vertebrae) collected for tissue fatty acid analysis and bone mineral density (BMD) determination. The analyses showed that long bones such as femur and tibia in DPAn-6-treated rats contained higher DPAn-6 content and generally had the lowest BMC and BMD values. Hence, DPAn-6 did not replace DHA for normal bone growth and maximal BMC in femur, indicating an indispensible role of DHA in bone health. In conclusion, DHA accumulates in the osteoblast-rich and nerve-abundant periosteum of femur; DHA but not EPA appears to be a vital constituent of marrow and periosteum of healthy modelling bone; and both DHA and total n-3 PUFA strongly correlate to BMC.

scholarly journals Serum phospholipid fatty acid pattern is associated with bone mineral density in children, but not adults, with cystic fibrosis

2006 ◽  
Vol 95 (6) ◽  
pp. 1159-1165 ◽  
Author(s):  
Eva Gronowitz ◽  
D Mellström ◽  
B Strandvik
Keyword(s):  
Bone Mineral Density ◽  
Cystic Fibrosis ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Lumbar Spine ◽  
Bone Mineral ◽  
Bone Growth ◽  
Fatty Acid Status ◽  
Mineral Density ◽  
Acid Status

Essential fatty acids (EFA) have proved to be important for normal bone mineral density (BMD) and bone growth in animal studies. Patients with cystic fibrosis often have low serum EFA levels, and low BMD has also been reported in patients with normal anthropometry. The aim of the present study was to analyse if BMD during a 2-year period was related to fatty acid status in patients with cystic fibrosis. Fifty-four patients, aged 6–33 years, were studied prospectively. BMD was measured with dual X-ray absorptiometry, and fatty acid concentrations in serum phospholipids were determined with capillary GLC. The cystic fibrosis patients showed low linoleic acid concentration and a high arachidonic acid (AA):DHA ratio in serum. The high eicosatrienoic acid:AA ratio, an indicator of EFA deficiency, increased further over 2 years, as did the total concentration of saturated fatty acids. In the adults there were no significant changes in fatty acids during the study. In the children, positive correlations were found between palmitic acid and bone mineral content in the lumbar spine and femoral neck. The lumbar spine BMDZscore correlated negatively with the AA:DHA ratio. No correlation was seen in adults except for a positive correlation between EFA deficiency index and the areas of lumbar spine and femoral neck. The present results imply that fatty-acid status influenced BMD in cystic fibrosis children, but not in adults, indicating that fatty-acid status wouldbe important for bone growth.

Effects of tower climbing exercise on bone mass, strength, and turnover in orchidectomized growing rats

2002 ◽  
Vol 93 (3) ◽  
pp. 1152-1158 ◽  
Author(s):  
Takuya Notomi ◽  
Yuichi Okazaki ◽  
Nobukazu Okimoto ◽  
Yuri Tanaka ◽  
Toshitaka Nakamura ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Formation ◽  
Bone Mass ◽  
Bone Mineral Content ◽  
Trabecular Bone ◽  
Bone Mineral ◽  
Mineral Content ◽  
Lumbar Vertebrae ◽  
Mineral Density ◽  
Sedentary Group

To determine the effects of a tower climbing exercise on mass, strength, and local turnover of bone, 70 9-wk-old Sprague-Dawley rats were assigned to seven groups: a baseline control and three groups of sham-operated sedentary, orchidectomized (ORX)-sedentary and ORX-exercise rats. Rats voluntarily climbed a 200-cm tower to drink water from a bottle set at the top. At 4 wk, the periosteal bone formation rate (BFR), moment of inertia, bone mineral content, bone mineral density, and bending load at the midfemur were maintained in ORX-exercise rats, whereas these parameters were reduced in ORX-sedentary rats. At 8 wk, the periosteal mineral apposition rate and BFR in ORX-exercise rats were significantly higher, whereas the parameters in ORX-sedentary rats did not differ compared with sham-sedentary rats. In ORX-exercise rats, the trabecular mineralizing surface, BFR, and bone volume of the lumbar vertebrae were maintained at the same levels as those in the sham-sedentary group, whereas the osteoclast surface decreased compared with the ORX-sedentary group. However, the climbing exercise did not affect bone mineral content, bone mineral density, or the compression load of the lumbar vertebrae. These results show that, in the midfemur, the voluntary climbing exercise maintained cortical bone mass and strength by stimulating periosteal bone formation and partially prevented ORX-induced trabecular bone loss, depressing the elevation of turnover. Interestingly, in ORX rats, the climbing exercise had the opposite effect on bone formation at the periosteal femoral cortical bone, where the exercise increased the bone formation compared with vertebral trabecular bone, where the exercise decreased it.

scholarly journals Promotion of Osteogenesis by Sweroside via BMP-2 Related Signaling in Postmenopausal Osteoporosis

2021 ◽  
Author(s):  
La Yoon Choi ◽  
Mi Hye Kim ◽  
Jae Min Han ◽  
Woong Mo Yang
Keyword(s):  
Bone Mineral ◽  
Osteoblast Differentiation ◽  
Mineral Content ◽  
Bone Matrix ◽  
Lumbar Vertebrae ◽  
Femoral Shaft ◽  
Bone Diseases ◽  
Mineral Density ◽  
Ovariectomized Mice ◽  
Mineralized Bone Matrix

Abstract Phlomis umbrosa has been traditionally used for bone diseases such as bone fracture and rheumatism in traditional Korea Medicine. Sweroside (SOS), which is one of the active compounds of P. umbrosa, has been known to promote osteoblast differentiation. In this study, ameliorative effects of SOS on osteoporosis and potential target pathway were investigated. Ovariectomized mice were administered 3 doses of SOS for 4 weeks after inducing osteoporosis. Bone mineral content (BMC) and bone mineral density (BMD) were analyzed by dual energy X-ray absorptiometry. SaOS-2 osteoblasts were differentiated to clarify the promoting effects of SOS on osteoblast differentiation and bone formation. Osteoblastic bone-forming markers were evaluated by RT-PCR in lumbar vertebrae (LV) and mineralized SaOS-2 cells. Treatment of SOS increased BMC and BMD levels. SOS markedly attenuated the bone marrow adipocytes in the central bone cavity of the femoral shaft. SOS increased the formation of bone matrix in SaOS-2 cells. BMP-2 and RUNX2 in LV and SaOS-2 cells were up-regulated by treating with SOS. BMP-2/RUNX2-activated ALP, OPN and BSP-1 expressions were increased by SOS. In conclusion, SOS induced the formation of mineralized bone matrix by regulating BMP-2/RUNX2-mediated osteoblastic molecules. Therefore, SOS could be a therapeutic active compound of treatment for osteoporosis by producing the new bone matrix.

Dietary-Related Skeletal Changes in a Shetland Sheepdog Puppy

2006 ◽  
Vol 42 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Chantal J. McMillan ◽  
Dominique J. Griffon ◽  
Steven L. Marks ◽  
Glenna E. Mauldin
Keyword(s):  
Bone Mineral Density ◽  
Renal Failure ◽  
Bone Mineral ◽  
Bone Growth ◽  
Dietary Calcium ◽  
Long Bone ◽  
Nutritional Management ◽  
Mineral Density ◽  
Limb Deformity ◽  
Skeletal Changes

A commercially available, renal failure diet was used to manage suspected renal failure in a 10-week-old Shetland sheepdog puppy. Rickets subsequently developed, possibly from low phosphorous intake and an increased calcium to phosphorus ratio. Decreased dietary calcium in addition to decreased phosphorus may have played a role in decreasing bone mineral density. Lethargy, decreased long bone growth, angular limb deformity, and osteopenia occurred, but these signs resolved within 3 months with nutritional management.

Influence of anthropometric parameters on assessment of paediatric bone mineral density and bone mineral content

2013 ◽  
Author(s):  
N Hangartner Thomas ◽  
F Short David ◽  
Gilsanz Vicente ◽  
J Kalkwarf Heidi ◽  
M Lappe Joan ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral Content ◽  
Bone Mineral ◽  
Mineral Content ◽  
Mineral Density ◽  
Anthropometric Parameters

scholarly journals Intra-Bone Marrow Administration of Mesenchymal Stem/Stromal Cells Is a Promising Approach for Treating Osteoporosis

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Hideki Agata ◽  
Yoshinori Sumita ◽  
Tatsuro Hidaka ◽  
Mayumi Iwatake ◽  
Hideaki Kagami ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Mineral Content ◽  
Bone Mineral ◽  
Intravenous Administration ◽  
Stromal Cells ◽  
Mineral Content ◽  
Bone Diseases ◽  
Research Progress ◽  
Bone Thickness ◽  
Mineral Density

Mesenchymal stem/stromal cells (MSCs) are known to be useful for treating local bone diseases. However, it is not known if MSCs are effective for treating systemic bone diseases, as the risk for mortality following intravenous MSC administration has hindered research progress. In this study, we compared the safety and efficacy of intra-bone marrow and intravenous administration of MSCs for the treatment of ovariectomy- (OVX-) induced osteoporosis. Cells capable of forming bone were isolated from the murine compact bones and expanded in culture. Relatively pure MSCs possessing increased potential for cell proliferation, osteogenic differentiation, and inhibition of osteoclastogenesis were obtained by magnetic-activated cell sorting with the anti-Sca-1 antibody. Sca-1-sorted MSCs were administered to OVX mice, which were sacrificed 1 month later. We observed that 22% of the mice died after intravenous administration, whereas none of the mice died after intra-bone marrow administration. With respect to efficacy, intravenous administration improved bone mineral density (BMD) by increasing bone mineral content without affecting bone thickness, whereas intra-bone marrow administration improved BMD by increasing both bone mineral content and bone thickness. These results indicate that intra-bone marrow administration of pure MSCs is a safer and more effective approach for treating osteoporosis.

Effects of One-Year Adjuvant Treatment with Tamoxifen on Bone Mineral Density in Postmenopausal Breast Cancer Women

1996 ◽  
Vol 82 (1) ◽  
pp. 65-67 ◽  
Author(s):  
Sandro Barni ◽  
Paolo Lissoni ◽  
Gabriele Tancini ◽  
Antonio Ardizzoia ◽  
Marina Cazzaniga
Keyword(s):  
Breast Cancer ◽  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mineral Content ◽  
Bone Mineral ◽  
Adjuvant Treatment ◽  
Mineral Content ◽  
Postmenopausal Breast Cancer ◽  
Mineral Density ◽  
One Year

In this study, the authors have analyzed the possible effects of one-year adjuvant treatment with tamoxifen on bone mineral density in postmenopausal breast cancer women. Bone mineral content was studied by photon absorptiometry (I-125), whereas bone balance was analyzed indirectly by serum PTH, osteocalcin, calcitonin, calcium and alkaline phosphatase levels. Bone mineral content and serum bone-related substances were measured before starting treatment and after one year. Results were analyzed using Student's t test for paired data. No difference was found between the two measurements for bone mineral content, PTH, calcitonin, calcium and alkaline phosphatase levels. Measurements at entry and after one year of treatment showed a statistically significant difference ( P < 0.001) only for osteocalcin. In accordance with other authors, we can conclude that treatment with tamoxifen does not cause an increase in menopausal bone resorption. The finding that osteocalcin levels decreased after one year of therapy with tamoxifen is interesting, but further studies are necessary to clarify the role of such levels in predicting a turnover of bone balance towards osteoblastic activity.

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