Examination of the isolated autosomes of the SKA strain of house-flies (Musca domestica L.) for resistance to several insecticides with and without pretreatment with sesamex and TBTP

1969 ◽  
Vol 59 (3) ◽  
pp. 409-421 ◽  
Author(s):  
R. M. Sawicki ◽  
A. W. Farnham
Keyword(s):  
Musca Domestica ◽  
High Resistance ◽  
The Other ◽  
Great Resistance ◽  
House Flies ◽  
Homozygous Condition ◽  
Resistance Factors ◽  
Slowing Down ◽  
Moderate Resistance ◽  
Esterase Inhibitor

The factors of resistance to many insecticides were located and isolated in a homozygous condition from the diazinon-selected SKA strain of house-flies (Musca domestica L.) by inbreeding each of the five autosomes of the SKA strain into a susceptible multi-marker strain ac;ar;bwb;ocra SRS. The insecticides were: DDT, methoxychlor, tri-butyl tin, the methoxy and ethoxy analogues of parathion, chlorthion and malathion and their corresponding phosphates, diazinon and diazoxon.The SKA flies' autosomes had the following factors of resistance:—autosome I—very slight resistance to ethyl-chlorthion; autosome II—gene a for low ali-esterase activity which conferred weak to moderate resistance against all organophosphates tested, and Deh (DDT-dehydrochlorinase) which gave great resistance to DDT, but none against methoxychlor; autosome III—Pen, which delayed knock-down by slowing down the entry of insecticides into the flies and had a negligible effect at death except against tri-butyl tin and methoxychlor, and a factor of resistance to Zectran unaffected by sesamex; autosome IV—Dld, the major factor of resistance to dieldrin; and autosome V—Ses, a sesamex-suppressed factor, which gave weak to moderate resistance against ethyl-malaoxon, diazoxon, diazinon, DDT and methoxychlor, but was ineffective against the other organophosphates tested.TBTP (S,S,S tri-butyl phosphorotrithioate), an ali-esterase inhibitor, greatly synergised organophosphates only against insects with gene a. Pretreatment with sesamex, an inhibitor of microsomal activity, elicited two types of response: antagonism with most of the thioates, most pronounced in flies with gene a, and synergism with the phosphates, especially evident with flies with factor Ses. The possible reasons for differences in the response to organophosphates after pretreatment with TBTP or sesamex, and the nature of the resistance factors are discussed. High resistance in SKA flies against organophosphates arises through the interaction of resistance factors which singly give only weak to moderate resistance.

scholarly journals Evaluation of triflumuron and pyriproxyfen as alternative candidates to control house fly, Musca domestica L. (Diptera: Muscidae), in Riyadh city, Saudi Arabia

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249496
Author(s):  
Saad M. Alzahrani
Keyword(s):  
Saudi Arabia ◽  
Musca Domestica ◽  
Laboratory Strain ◽  
Insect Growth Regulator ◽  
House Fly ◽  
House Flies ◽  
Resistance Factors ◽  
Control Programs ◽  
Riyadh City ◽  
Susceptibility And Resistance

This study was conducted to determine the susceptibility and resistance of some house fly strains of Musca domestica L. to the insect growth regulator insecticides triflumuron and pyriproxyfen in some locations in Riyadh city. Field-collected strains of M. domestica L. from five sites in Riyadh city that represented five slaughterhouse sites where flies spread significantly were tested against triflumuron and pyriproxyfen. Triflumuron LC50 values for the five collected strains ranged from 2.6 to 5.5 ppm, and the resistance factors (RFs) ranged from 13-fold to 27-fold that of the susceptible laboratory strain. Pyriproxyfen LC50 values for the field strains ranged from 0.9 to 1.8 ppm with RFs of 3-fold to 5-fold. These results indicate that pyriproxyfen is an effective insecticide to control house flies and should be used in rotation with other insecticides in the control programs applied by Riyadh municipality.

Resistance status and the rate of resistance development in Musca domestica Linnaeus (Diptera: Muscidae) to permethrin and imidacloprid in Indonesia

2021 ◽  
Vol 18 (3) ◽  
pp. 159-169
Author(s):  
Sri Yusmalinar ◽  
◽  
Tjandra Anggraeni ◽  
Ramadhani Eka Putra ◽  
Ashari Zain ◽  
...  
Keyword(s):  
Musca Domestica ◽  
High Resistance ◽  
Management Strategies ◽  
Repeated Application ◽  
Resistance Level ◽  
Resistance Development ◽  
Development Of Resistance ◽  
Pest Infestation ◽  
Moderate Resistance ◽  
Very High

A previous report has indicated that in many regions of Indonesia, populations of Musca domestica Linnaeus have shown very high resistance to permethrin and low resistance to imidacloprid. In this study, the resistance status to permethrin and imidacloprid was updated using a topical application and feeding bioassay. Six housefly strains originated from six highly populated cities in Indonesia, namely Serang (SRG), Jakarta (JKT), Bandung (BDG), Semarang (SMG), Yogyakarta (JOG), and Surabaya (SBY). A seventh strain (Danish Pest Infestation Laboratory (DPIL)) served as the control. Each strain was tested for resistance to the two insecticides. In addition, the rate of development of resistance to the two insecticides was measured over ten generations. The results indicated that all field strains showed very high resistance to permethrin. The highest resistance level recorded was in the SRG strain (RR50 = 2880), and the lowest was in the JKT strain (RR50 = 520). Repeated application of permethrin over ten generations increased the resistance level by about 2.7–32.73-fold as compared to the level of their respective parental populations. On the other hand, most strains showed low to moderate resistance to imidacloprid, in which the SRG strain had the highest resistance level (RR50 = 15.5) and the SBY strain had the lowest (RR50 = 2.0). Repeated application of imidacloprid over ten generations increased the resistance level by about 3.25–17.41-fold. The findings, which is the second report of housefly resistance in Indonesia since 2016, provide a crucial foundation for developing appropriate housefly integrated pest management strategies in highly populated areas in Indonesia.

A dipping technique for selecting house-flies, Musca domestica L., for resistance to insecticides

1964 ◽  
Vol 55 (3) ◽  
pp. 541-546 ◽  
Author(s):  
R. M. Sawicki ◽  
A. W. Farnham
Keyword(s):  
Musca Domestica ◽  
Topical Application ◽  
Filter Paper ◽  
Coefficient Of Variation ◽  
Probit Regression ◽  
House Flies ◽  
Resistance Factors ◽  
Sintered Glass ◽  
Large Numbers ◽  
Resistant Strains

A dipping technique for exposing large numbers of house-flies (Musca domestica L.) to measured doses of insecticide is described. It is suitable for selecting resistant populations and, while giving consistent results, is more rapid than other techniques used for this purpose.Up to 2,000 flies of both sexes, less than 24 hr. old, are immersed for three minutes in 100 ml. of a 70 per cent, mixture of acetone and water containing the required concentration of insecticide, using a 9-cm. sintered glass Büchner funnel as the immersion chamber. The liquid is then removed by suction, the sides of the funnel are wiped with filter paper, and the flies are allowed to drain for three minutes; they are then transferred in small batches to plastic recovery chambers containing food. Mortality is recorded next day, and the survivors are released into breeding cages.Experiments showed that immersion for three minutes in 70 per cent, acetone was virtually harmless to the flies and that the amount of insecticide deposited on individual flies was reasonably uniform (coefficient of variation about 20%). Batches of 2,000 flies, but not more, could be treated at one time.When the dipping technique was compared with topical application of measured drops of insecticide, using a susceptible strain of house-flies and two other strains that were resistant to DDT and diazinon, dipping gave steeper log-probit regression lines than topical application, and the LD50's and resistance factors of the resistant strains were smaller. With flies resistant to DDT, dipping gave straight regression lines whereas topical application gave compound lines.

MODELLING OVIPOSITION OF THE HOUSE FLY (DIPTERA: MUSCIDAE)

1991 ◽  
Vol 123 (2) ◽  
pp. 345-352 ◽  
Author(s):  
T.J. Lysyk
Keyword(s):  
Musca Domestica ◽  
Ovarian Development ◽  
House Fly ◽  
The Other ◽  
Cumulative Probability ◽  
House Flies ◽  
Development Rates ◽  
Simulation Results ◽  
The Relationship ◽  
Ovarian Cycles

AbstractThe relationship between temperature and ovarian development rates, as well as the cumulative probability of a female becoming gravid, were determined for the house fly, Musca domestica (L.), based on previously published information. These relationships were combined to form a model that simulates oviposition of house fly populations using average daily temperatures, eggs per cycle, and daily survival as input. Simulation results were compared with observed oviposition in three caged populations of house flies exposed to field temperatures. A high correlation occurred between simulated and observed eggs laid (r = 0.88), as well as between simulated and observed lxmx (r = 0.91). The model overestimated the onset of oviposition for one population, but closely simulated the timing of oviposition for the other two. The model also tended to overestimate the reproductive contribution of flies during the second and subsequent ovarian cycles.

Factors affecting resistance to insecticides in house-flies, Musca domestica L. (Diptera: Muscidae). II. Close linkage on autosome 2 between an esterase and resistance to trichlorphon and pyrethroids

1984 ◽  
Vol 74 (2) ◽  
pp. 197-206 ◽  
Author(s):  
R. M. Sawicki ◽  
A. L. Devonshire ◽  
A. W. Farnham ◽  
Kate E. O'Dell ◽  
G. D. Moores ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Pyrethroid Resistance ◽  
Resistance Mechanism ◽  
Mechanisms Of Resistance ◽  
Pyrethroid Insecticides ◽  
Factors Affecting ◽  
House Flies ◽  
Resistant Strains ◽  
Moderate Resistance ◽  
Weak Resistance

AbstractWidespread slight pyrethroid-resistance in Musca domestica L. on animal farms in southern England was correlated with strong resistance to trichlorphon and to malathion, and with the presence of an esterase, E0·39, detected by electrophoresis. In the laboratory, the frequency of E0·39 increased in response to selection with either pyrethroids or trichlorphon. Genetic analysis confirmed that this esterase, controlled by a gene on autosome 2, was closely linked with moderate resistance to trichlorphon and malathion and weak resistance to pyrethroids. When autosome 2 with the gene for E0·39 was introduced into a strain homozygous for the resistance mechanism super-kdr, resistance to pyrethroids increased by a factor equivalent to the weak resistance conferred by autosome 2 with E0·39 alone. Homozygosity for both mechanisms of resistance, and E0·39, was obtained by selecting the progeny of this cross with permethrin alone, permethrin and trichlorphon, or DDT and trichlorphon, demonstrating that very strong pyrethroid resistance can be achieved through the use of non-pyrethroid insecticides. E0·39 was absent from insecticide-resistant strains of M. domestica from Denmark but was present in several multi-resistant strains from other European countries.

Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Effective Concentration ◽  
P Value ◽  
Piper Betle ◽  
Microbiology Laboratory ◽  
House Flies ◽  
Betel Leaf ◽  
The One ◽  
One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies

CONTROL OF EGG FERTILIZATION BY NASONIA VITRIPENNIS (HYMENOPTERA: PTEROMALIDAE) WHEN LAYING ON PARASITIZED HOUSE FLY PUPAE

1973 ◽  
Vol 105 (5) ◽  
pp. 709-718 ◽  
Author(s):  
H. G. Wylie
Keyword(s):  
Musca Domestica ◽  
House Fly ◽  
Nasonia Vitripennis ◽  
House Flies

AbstractFemales of Nasonia vitripennis (Walk.) lay a smaller percentage of fertilized (i.e. female) eggs on house fly, Musca domestica L., pupae previously parasitized by their own species, by Muscidifurax zaraptor K. & L., or by Spalangia cameroni Perk. (Hymenoptera: Pteromalidae) than on unparasitized hosts. They respond to changes in the fly pupae associated with death, and in the case of house flies attacked by N. vitripennis, to "venoms" injected at that time or to changes unrelated to death. By not fertilizing eggs that they lay on attacked hosts, the females also conserve sperm, for immature N. vitripennis on previously-attacked fly pupae are usually killed by parasite larvae already present.

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