scholarly journals Morphological and histochemical variations of mucous and oxynticopeptic cells in the stomach of the seps, Chalcides chalcides

1999 ◽  
Vol 194 (1) ◽  
pp. 71-77
Author(s):  
DOMENICO FERRI ◽  
GIUSEPPA ESTERINA LIQUORI ◽  
GIOVANNI SCILLITANI
Keyword(s):  
Proteolytic Enzymes ◽  
Lectin Histochemistry ◽  
Histochemical Staining ◽  
Mucous Cells ◽  
Zymogen Granules ◽  
Con A ◽  
Fundic Mucosa ◽  
Acid Secreting ◽  
Staining Methods ◽  
Oxynticopeptic Cells

Mucous and oxynticopeptic cells in the gastric mucosa of the seps, Chalcides chalcides (Linnaeus, 1758) were examined by standard histochemical staining methods and by lectin histochemistry. The epithelial mucous cells lining the surface of the stomach and the mucous cells of the fundic glands elaborated mainly neutral glycoproteins with β(1,4)GlcNAc oligomers, GalNAc glycosidic residues and Galβ1,3GalNAc terminal sequences. The mucous cells of the fundic glands were stained specifically with the Paradoxical Con A method. The mucosecreting cells of the pyloric glands produced neutral glycoproteins, with β(1,4)GlcNAc oligomers, GalNAc residues and Galβ1,3GalNAc terminal sequences. Terminal L-fucose bound to the penultimate GlcNAc residues, and/or difucosylated oligosaccharides were also present. The pyloric glands did not stain with the Paradoxical Con A procedure. The morphology of the oxynticopeptic cells changes from the oral to the aboral region of the fundic mucosa. In the oral fundic tract the oxynticopeptic cells showed cytoplasm filled with zymogen granules, while in the aboral fundic region these cells contained few zymogen granules and showed cytoplasm full of empty vesicles, typical of the acid secreting cells. A secretion gradient of proteolytic enzymes and hydrochloric acid along the fundic mucosa of the seps can be hypothesised.

scholarly journals Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis

Pathobiology ◽  
2015 ◽  
Vol 82 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Daniela Cabibi ◽  
Vincenza Calvaruso ◽  
Letizia Giuffrida ◽  
Sabrina Ingrao ◽  
Laura Balsamo ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Transient Elastography ◽  
Histochemical Staining ◽  
Staining Methods

scholarly journals Spontaneous poisoning by Sida carpinifolia (Malvaceae) in horses

2017 ◽  
Vol 37 (9) ◽  
pp. 926-930 ◽  
Author(s):  
Daniele M. Bassuino ◽  
Guilherme Konradt ◽  
Matheus V. Bianchi ◽  
Matheus O. Reis ◽  
Saulo P. Pavarini ◽  
...  
Keyword(s):  
Neurodegenerative Disorder ◽  
Neurological Diseases ◽  
Clinical Signs ◽  
Lectin Histochemistry ◽  
Lysosomal Storage ◽  
Con A ◽  
Indolizidine Alkaloids ◽  
The Central Nervous System ◽  
Progressive Weight Loss ◽  
Gross Lesions

ABSTRACT: Sida carpinifolia poisoning causes a chronic neurodegenerative disorder associated with lysosomal storage by indolizidine alkaloids (swainsonine). The epidemiological, clinical, pathological and lectin histochemistry findings of an outbreak of natural poisoning by S. carpinifolia in horses in Rio Grande do Sul state, Brazil, are described. Five horses from a total of 15 that were kept on native pasture with large amounts of S. carpinifolia presented during 90 days clinical signs of progressive weight loss, incoordination, stiff gait and ramble, in addition to exacerbated reactions and locomotion difficulty after induced movement. Four horses died, and one of them was submitted for necropsy. At necropsy, no significant gross lesions were observed. Histological findings observed in the central nervous system were characterized by swollen neurons with cytoplasm containing multiple microvacuoles; these abnormalities were more severe in the thalamus, hippocampus, cerebellum and pons. Using lectin histochemistry, the pons and hippocampus sections stained positive for commercial lectin Con-A, sWGA and WGA. This study aimed to detail S. carpinifolia poisoning in horses to be included in the differential diagnoses of neurological diseases of horses.

scholarly journals Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae

2017 ◽  
Vol 61 (3) ◽  
Author(s):  
Gianluca Accogli ◽  
Giovanni Scillitani ◽  
Donatella Mentino ◽  
Salvatore Desantis
Keyword(s):  
Surface Layer ◽  
Lectin Histochemistry ◽  
The Body ◽  
Octopus Vulgaris ◽  
Mucous Cells ◽  
Cell Type ◽  
Skin Mucus ◽  
The Common ◽  
Common Octopus

<p>The O<em>ctopus vulgaris</em> farming is impaired by the high mortality of the paralarvae during the first month of life. Several factors have been investigated in this regard, but no data exist on the body surface mucus, which represents the interface with the outside environment. This study included morphometric analysis and glycoconjugates characterization of skin mucus in reared <em>Octopus vulgaris</em> paralarvae during the first month of life. Four types of mucous cells were distinguished:  mucous 1 (m1) and mucous 2 (m2) cells were scattered in the mantle epidermis, mucous 3 (m3) and mucous 4 (m4) in the epithelium surrounding the sucker. Except for the presence of fucosylated and neutral glycoconjugates in all mucous cells, each cell type expressed a characteristic glycopattern. m2 and m4 contained also suphate and acid non-sulphate glycans, m3 lacked suphate glycoproteins. Lectin histochemistry showed that mantle mucous cells (m1, m2) expressed GlcNAc and lactosamine terminating glycans. m2 also contained GalNAc terminal or penultimate to sialic acid. m3 was distinguished by mannosylated glycans terminating with lactosamine and m4 by α2,6 sialoglycans. Glycoproteins terminating with lactosamine, Galβ1,3GalNAc, and α1,6-linked fucose were a common feature of paralarvae surface layer. Morphometry revealed a significant decrease of m1 and m2 abundance during the first month of life, afterwards the reared paralarvae died. Since the glycopattern did not change during the investigated period, the mantle mucous cells abundance could be related to the <em>Octopus vulgaris</em> paralarvae survival. </p>

scholarly journals Amyloid in Canine Mammary Tumors

1985 ◽  
Vol 22 (4) ◽  
pp. 347-354 ◽  
Author(s):  
J. H. Vos ◽  
E. Gruys
Keyword(s):  
Epithelial Cells ◽  
Mammary Tumors ◽  
Histochemical Staining ◽  
Amyloid Deposition ◽  
Corpora Amylacea ◽  
Mammary Carcinomas ◽  
Canine Mammary Tumors ◽  
Stromal Tissue ◽  
Staining Methods ◽  
Neoplastic Epithelial Cells

In canine mammary carcinomas, amyloid was present as amyloid-containing corpora amylacea and as local deposits between neoplastic epithelial cells or in stromal tissue. Histochemical staining methods revealed that this amyloid was not of the AA-type amyloid and contained tryptophan. The possible pathogenesis of this amyloid deposition is discussed.

Uneven distribution of preservative in kiln-dried sapwood lumber of Scots pine: Impact of wood structure and resin allocation

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Sheikh Ali Ahmed ◽  
Margot Sehlstedt-Persson ◽  
Olov Karlsson ◽  
Tom Morén
Keyword(s):  
Scots Pine ◽  
Wood Preservative ◽  
Wood Properties ◽  
Histochemical Staining ◽  
Kiln Drying ◽  
Specific Distribution ◽  
Ray Cells ◽  
Staining Methods ◽  
Staining Techniques ◽  
Resin Canals

Abstract Scots pine (Pinus sylvestris L.) sapwood lumber was collected after kiln drying and preservative treatment with Celcure AC 800 (a copper-amine wood preservative). Distribution of the preservative throughout the lumber was visually examined. Not all, but some samples showed specific localized areas without any preservative distribution throughout their entire length. Those samples were assessed further for anatomical properties, specifically in impregnated and unimpregnated areas. Additional study was conducted on the morphological nature and redistribution of lipophilic extractives using three different histochemical staining methods. Intrinsic wood properties – especially the frequency of axial resin canals and the percentage of canals blocked – were found to be responsible for the irregular distribution of the preservative. Furthermore, the inability to create continuous and frequent interstitial spaces due to the collapse of thin-walled ray cells throughout the lumber resulted in un-even distribution of preservatives. Staining techniques were useful to localize places with more or less abundance of extractives (e.g., fats) in impregnated and unimpregnated wood, which varied considerably. Histochemical observations revealed information pertaining to the kiln dry specific distribution and redistribution of extractives between the areas. Moreover, resin reallocation and modification in ray parenchyma and resin canals induced by kiln drying would be another reason for the impregnation anomalies.

scholarly journals The islet ghrelin cell

2013 ◽  
Vol 52 (1) ◽  
pp. R35-R49 ◽  
Author(s):  
Nils Wierup ◽  
Frank Sundler ◽  
R Scott Heller
Keyword(s):  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Developmental Regulation ◽  
Genetic Regulation ◽  
Cell Types ◽  
Human Islets ◽  
Histochemical Staining ◽  
Pancreatic Tumours ◽  
Age Related ◽  
Staining Methods

The islets of Langerhans are key regulators of glucose homeostasis and have been known as a structure for almost one and a half centuries. During the twentieth century several different cell types were described in the islets of different species and at different developmental stages. Six cell types with identified hormonal product have been described so far by the use of histochemical staining methods, transmission electron microscopy, and immunohistochemistry. Thus, glucagon-producing α-cells, insulin-producing β-cells, somatostatin-producing δ-cells, pancreatic polypeptide-producing PP-cells, serotonin-producing enterochromaffin-cells, and gastrin-producing G-cells have all been found in the mammalian pancreas at least at some developmental stage. Species differences are at hand and age-related differences are also to be considered. Eleven years ago a novel cell type, the ghrelin cell, was discovered in the human islets. Subsequent studies have shown the presence of islet ghrelin cells in several animals, including mouse, rat, gerbils, and fish. The developmental regulation of ghrelin cells in the islets of mice has gained a lot of interest and several studies have added important pieces to the puzzle of molecular mechanisms and the genetic regulation that lead to differentiation into mature ghrelin cells. A body of evidence has shown that ghrelin is an insulinostatic hormone, and the potential for blockade of ghrelin signalling as a therapeutic avenue for type 2 diabetes is intriguing. Furthermore, ghrelin-expressing pancreatic tumours have been reported and ghrelin needs to be taken into account when diagnosing pancreatic tumours. In this review article, we summarise the knowledge about islet ghrelin cells obtained so far.

scholarly journals The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kenan Çinar ◽  
Mustafa Öztop ◽  
Emel Demirbağ
Keyword(s):  
Regional Distribution ◽  
Distribution Patterns ◽  
Histochemical Staining ◽  
Slight Reduction ◽  
Eisenia Foetida ◽  
Mucous Cells ◽  
Functional Roles ◽  
Comprehensive Information ◽  
Reaction Intensity

The aim of this study was to characterize the nature and regional distribution of the glycoconjugates secreted by epidermal mucous cells in Eisenia foetida (Annelida). Specimens were divided into six regions from anterior to posterior. The histochemistry was carried out by using standard histochemical methods. Histochemical staining properties of glycoconjugates in epidermal mucous cells were determined regionally. The epidermis of all regions contained strong to stronger PAS (+) cells in various degrees. The epidermis of the first, fourth, fifth, and sixth regions had strong to stronger AB pH 2.5 (+) cells. On the contrary, all regions contained weak to moderate AB pH 0.5 and AB pH 1.0 (+) cells. Most of mucous cells in epidermis of the first region contained both PAS (+) and AB (+) mucosubstances. All regions included weaker to weak AF (+) cells. All regions featured KOH/PAS (+) cells, with a slight reduction in reaction intensity in the epidermis of the last three regions. In this context, the different staining patterns observed in epidermal mucous cells hinted at their functional roles with respect to production of mucus with different physical properties. This study provided comprehensive information about the regional distribution patterns of the glycoconjugates and an opportunity to compare their distributional patterns in other annelids.

scholarly journals A QUANTITATIVE HISTOCHEMICAL STUDY OF INTESTINAL MUCOSA AFTER X-IRRADIATION

1970 ◽  
Vol 18 (4) ◽  
pp. 291-301 ◽  
Author(s):  
H. GALJAARD ◽  
J. BUYS ◽  
M. VAN DUUREN ◽  
J. GIESEN
Keyword(s):  
Esterase Activity ◽  
Histochemical Staining ◽  
Nonspecific Esterase ◽  
Radiation Doses ◽  
Functional Consequences ◽  
X Irradiation ◽  
Staining Methods ◽  
Quantitative Analyses ◽  
Nonspecific Esterase Activity ◽  
Crypt Cells

The effect of low doses of x-irradiation (50-400 R) on the activity of various enzymes in rat intestinal epithelium has been investigated by histochemical staining methods and quantitative microchemical analyses of crypts and villi dissected from frozen-dried sections. Irradiation had no effect on the activities of enzymes which in nonirradiated animals are present exclusively or mainly in the villus epithelium (aminopeptidase, various phosphatases) or are evenly distributed along the epithelium of crypts and villi (various dehydrogenases). However, nonspecific esterase activity decreased markedly both in crypt epithelium and villus epithelium. This occurred 36 hr after irradiation, independent of the radiation dose. The number of crypt cells with reduced esterase activity and the duration of the effect increased with higher radiation doses. These results were confirmed by quantitative analyses which also showed that esterase activity is 5 times higher in the villus than in the crypt. The remarkable correspondance between the period of reduced esterase activity in the crypt and the period of increased proliferative activity after various radiation doses suggest a relationship between changes in crypt cell population dynamics and esterase activity; the functional consequences for the villus epithelium of changes in the crypt cells after irradiation are discussed.

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