Antioxidant and cholesterol assimilation activities of selected lactobacilli and lactococci cultures

2009 ◽  
Vol 76 (4) ◽  
pp. 385-391 ◽  
Author(s):  
Shalini Jain ◽  
Hariom Yadav ◽  
Pushpalata Ravindra Sinha
Keyword(s):  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Radical Scavenging ◽  
Fermented Milk ◽  
Blood Cholesterol ◽  
Peroxide Radical ◽  
Bacterial Cultures ◽  
Faecal Samples

In present study, three strains of lactic acid bacteria (LAB) viz.Lactobacillus casei,Lactobacillus acidophilusandLactococcus lactisand milk fermented with these strains have been studied for antioxidant and cholesterol assimilation activities in-vitro and in-vivo, in addition to the effect on total lactobacilli, lactococci and coliform counts into the gut of mice fed with diets supplemented by fermented milk. All three selected strains exhibited potent 2,2-diphenyl-1-picrylhydrazyl, malonaldialdehyde and hydrogen peroxide radical scavenging abilities as well as inhibition of linoleic acid peroxidation activity. These activities were highest inLb. caseias followed byLb. acidophilusandLc. lactis. In addition, these bacterial cultures also exhibited good in-vitro cholesterol assimilation potential. Oral administration to mice of milk fermented with selected LAB strains, slightly decreased blood cholesterol, increased colonization of total lactobacilli and lactococci, and decreased coliforms in the intestinal tissues as well as faecal samples. These results indicate that, selected LAB strains have good antioxidant, hypocholesterolemic and coliform removal activities. It may suggest that, a novel functional food can be obtained by supplementation of selected LAB in milk, which may have various health beneficial properties such as antioxidant and hypocholesterolemic activities.

Effect of a Mixture of Lactobacillus casei and Lactobacillus acidophilus Administered Orally on the Immune System in Mice

1986 ◽  
Vol 49 (12) ◽  
pp. 986-989 ◽  
Author(s):  
GABRIELA PERDIGON ◽  
MARIA ELENA NADER de MACIAS ◽  
SUSANA ALVAREZ ◽  
MARTA MEDICI ◽  
GUILLERMO OLIVER ◽  
...  
Keyword(s):  
Immune System ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Lymphocyte Activation ◽  
Fermented Milk ◽  
Peritoneal Macrophages ◽  
Oral Route ◽  
Carbon Clearance ◽  
Stimulation Of

The effects of an orally-administered mixture of Lactobacillus casei and Lactobacillus acidophilus on the immune system in Swiss albino mice were studied. Non-fermented milk containing viable cultures of both microorganisms was fed for different consecutive days to the animals, the effect of such feeding on their immune system was evinced by macrophage and lymphocyte activation. An increase both in the in vitro phagocytic activity of peritoneal macrophages and in the carbon clearance activity was observed. As regard the lymphocytic activity, the mixture produced a higher activation than that in the control mice. The enhanced macrophage and lymphocytic activity by administering cultures via the oral route, suggest the advisability of using the mixture of bacteria for a more efficient stimulation of the host immune response.

scholarly journals Digestão Simulada de Bebida Esportiva Contendo Diferentes Probióticos

2020 ◽  
Vol 24 (1) ◽  
pp. 47-53
Author(s):  
Luciana Carvalho Fino ◽  
Fabíola Lima Pena ◽  
Maria Carolina Pelatieri Valle ◽  
Thais Ramos Silva ◽  
Vivian Cristina Cruz Rodrigues ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Lactobacillus Rhamnosus ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Pomegranate Juice ◽  
Sports Drink ◽  
Bifidobacterium Animalis

O exercício físico de alta intensidade está relacionado com diversas alterações sistêmicas que podem prejudicar a performance do indivíduo, tais como: a queda da imunidade, a perda de massa muscular, a insônia, entre outros. Os probióticos, quando administrados regularmente para esse público, podem reduzir os sintomas relacionados ao overtraining. O presente estudo buscou selecionar uma cepa probiótica para empregar em uma bebida esportiva (a base de leite fermentado), contendo elevada quantidade de compostos fenólicos e adicionada de cepas probióticas, sendo utilizadas como principal critério de escolha a sobrevivência da mesma após digestão simulada. Foram avaliados os probióticos Lactobacillus casei (BGP93), Lactobacillus rhamnosus (SP1) e Lactobacillus acidophilus (LA3) e Bifidobacterium animalis subsp. lactis (BB12), utilizando-se como cultura starter o Streptococcus thermophilus (TA072). Em paralelo foi realizada avaliação da resistência das culturas probióticas em solução adicionada de suco de romã, tendo em vista a potencial atividade antimicrobiana desta fruta. Neste teste foi observado que as culturas probióticas não foram inibidas pelo suco de romã, com exceção do L. acidophilus LA3. Para digestão in vitro foi utilizado protocolo validado composto pelas fases gástrica e entérica, a viabilidade dos probióticos na bebida esportiva foi acompanhada durante armazenamento refrigerado a 6 °C por 28 dias e a sobrevivência foi avaliada, por meio de contagem seletiva em DeMan, Rogosa & Sharpe (MRS) ágar acidificado e incubação em anaerobiose. Os resultados demonstraram que o Lactobacillus casei cepa BGP93 apresentou adequada viabilidade durante o armazenamento e maior taxa de sobrevivência à digestão, quando comparado aos demais probióticos, representando um bom candidato para a elaboração da bebida esportiva. Palavras chave: Leite Fermentado. Compostos Fenólicos. Alimento Funcional. Viabilidade de Probióticos.    Abstract High-intensity physical exercise is related to several systemic alterations that may impair the individual's performance, such as immunity depletion, muscle mass loss, insomnia, among others. Probiotics, when given regularly to this public, may reduce symptoms related to overtraining. The present study aimed to select a probiotic strain for use in a sports drink (based on fermented milk) containing a high amount of phenolic compounds, and added probiotic strains, being the main criterion of choice the survival of the strain after simulated digestion. The probiotics Lactobacillus casei (BGP93), Lactobacillus rhamnosus (SP1) and Lactobacillus acidophilus (LA3) and Bifidobacterium animalis subsp. lactis (BB12), using as starter culture Streptococcus thermophilus (TA072). At the same time, resistance evaluation of probiotic cultures in pomegranate juice solution was carried out, considering the potential antimicrobial activity of this fruit. In this test, it was observed that probiotic cultures were not inhibited by pomegranate juice, except for L. acidophilus LA3. For in vitro digestion a validated protocol consisting of gastric and enteric phases was used, the viability of probiotics in the sports drink was monitored during cold storage at 6 ° C for 28 days and survival was evaluated by selective counting in acidified DeMan, Rogosa & Sharpe (MRS) agar and incubation in anaerobiosis. The results showed that Lactobacillus casei strain BGP93 showed adequate viability during storage and higher survival rate to digestion when compared to other probiotics, representing a good candidate for the sport drink preparation Keywords: Fermented Milk. Phenolic Compounds. Functional Food. Probiotics Viability.

scholarly journals Comparative Study of Commercial Starter and Gum-producing Lactic Acid Bacteria Isolated from Palm-sap on Yoghurt

2020 ◽  
pp. 28-39
Author(s):  
Oniovosa Leonard Adamu- Governor ◽  
Peter Nwabueze Okolie ◽  
Emmanuel Mmmaduabuchi Ikegwu ◽  
Ifedinma Henrietta Obeten ◽  
Christiana Oluwatoyin Abiona ◽  
...  
Keyword(s):  
Lactobacillus Acidophilus ◽  
Storage Period ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Cow Milk ◽  
Significant Difference

Yoghurt remains a fermented milk of choice globally but its desirability is limited by quality attributes and syneresis. In this study, the effect of using exopolysaccharide (EPS) producing starter cultures and EPS on the quality attribute of yoghurt produced from cow milk was examined. Two starter cultures of EPS-producing LAB were used singly and in combination in three treatments portions (YEPSLa, Lactobacillus acidophilus yoghurt; YEPSLs, Leuconostoc suionicum; YEPSLa + YEPSLs, Lactobacillus acidophilus and Leuconostoc suionicum); Yxg, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus to produced yoghurt while reference yoghurt (RY) was obtained from the market and refrigerated stored at 4oC. Sensory, proximate composition, textural, rheological properties and whey separation were carried out after 1 and 28 days only, while physicochemical and microbiological were analyzed after 1, 7, 14 and 28 days. No significant difference (p ˂0.05) between RY (8.60 ± 0.60), (7.21±0.10) and YEPSLa + YEPSLs (8.54 ± 0.71), (7.25 ± 0.21) in overall acceptability for day 1 and 28. Moisture (82.45 ± 0.12 - 81.31 ± 0.06%), fat (3.46 ± 0.01 - 3.42 ± 0.03%) and carbohydrate (13.05 ± 0.11 to 12.51 ± 0.10%) contents decreased while total solids (17.57 ± 0.12 - 17.97 ± 0.12%), ash (0.56 ± 0.02 - 0.57 ± 0.02%) and protein (3.74 ± 0.01 - 4.30%) contents increased respectively across the yoghurts. The result showed that the highest cohesiveness and syneresis was observed in YEPSLa + YEPSLs (27.52 ± 0.63) and commercial yoghurt (29.10 ± 0.31), the lowest in Yxg (16.71 ± 0.21) and YEPSLa + YEPSLs (21.50 ± 0.51). The highest viscosity was observed in YEPSLa + YEPSLs across the rotation speeds.  The pH and titratable acid ranged (4.28 – 4.50; 0.90 – 1.41) while the total bacteria colony count (5.5×108 – 11.0×108cfu/ml) during 28 days storage period. Overall, EPS produced by EPS-producing LAB both In-vitro and In-vivo improve texture, mouthfeel, viscosity and reduce syneresis in yoghurt. Combine cultures of Lactobacillus acidophilus and Leuconostoc suionicum and their EPSs competed favourably with conventional starter, and other stabilizing agents in cow milk yoghurt.

Hepato- and Nephroprotective Effects of the Polyphenol Concentrate From Cabernet Sauvignon Grape Varieties Cultivated in Kazakhstan in the Experimental Model Of CCL4-Induced Toxicity

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Nurgozhin T. ◽  
Sergazy S. H. ◽  
Adilgozhina G. ◽  
Gulyayev A. ◽  
Shulgau Z. ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Experimental Model ◽  
Lethal Dose ◽  
Radical Scavenging ◽  
Cabernet Sauvignon ◽  
Intragastric Administration ◽  
Liver And Kidney ◽  
Antioxidant Stress

Objective:This study investigates the hepatoprotective effect and the antioxidant role of polyphenol concentrate in the experimental model of carbon tetrachloride (CCl4) induced toxicity. Methods: Antioxidant activity of Cabernet Sauvignon grape polyphenol were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), 2,2’-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+). In addition, the effects of polyphenol concentrate on the survival of Wistar rats in the toxicity model, was also investigated. The polyphenol concentrate was administered for 5 five days prior to injection of carbon tetrachloride in a sub-lethal dose of 300 mg/kg of animal body weight in order to perform histological examinations of the liver and kidney, and detect the levels of AST, ALT and bilirubin. Results: Administration of polyphenol concentrate increased animal survival in the experimental model. Moreover, the intragastric administration of polyphenol concentrate prior to the initiation of the experimental model of toxicity, which was caused by a sub-lethal CCl4 dose, reduced morphological injuries in the liver and kidney, decreased the AST and ALT levels of the blood serum. Discussion and conclusion: Our data demonstrate that polyphenol concentrate possesses an antioxidant potential both in vitro and in vivo by reducing antioxidant stress that was caused by CCl4 administration into rats.

VIABILITAS LACTOBACILLUS ACIDOPHILUS DALAM PAKAN AYAM BROILER UNTUK MENGHAMBAT PENYAKIT PULLORUM

2018 ◽  
Vol 16 (1) ◽  
pp. 47-52
Author(s):  
Ida Ningrumsari ◽  
Lina Herlinawati
Keyword(s):  
Lactobacillus Acidophilus ◽  
Salmonella Pullorum

Penyakit pullorum dikenal dengan nama berak kapur atau berak putih (Bacilary white Diarrchae)yang banyak menimbulkan kerugian bagi peternak, oleh karena itu dilakukan penelitian dengantujuan untuk mengetahui ketahanan (viabilitas) L acidophilus dalam pakan ayam broiler untukmenghambat penyakit pullorum. Rancangan penelitian menggunakan eksperimentallaboratorium, persamaan kuadratik dan Rancangan Acak Lengkap (RAL) 1 faktorial dengan polaperlakuan konsentrasi L acidophilus dari 106-109 . Pertumbuhan terbaik dari L acidophilus yaituyang berumur 12 jam digunakan untuk menghambat Salmonella pullorum,sedangkanpertumbuhan S pullorum yang dapat menginfeksi ayam yaitu pada umur 15 jam.KonsentrasiL acidophilus yang dapat menghambat S pullorum secara in vitro yaitu 107, LD50 Salmonellapullorum in vivo ayam broiler pada 108. Viabilitas (ketahanan) L acidophilus dalam pakan bisabertahan hidup di atas 35 hari.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro

2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Ganiyu Oboh ◽  
Veronica O. Odubanjo ◽  
Fatai Bello ◽  
Ayokunle O. Ademosun ◽  
Sunday I. Oyeleye ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Seed Extract ◽  
Persea Americana ◽  
Dependent Manner ◽  
Natural Treatment

AbstractAvocado pear (The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of FeThe extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID.The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.

Nanostructured Ethosomal Gel Loaded with Arctostaphylos uva-ursi extract; In-vitro/In-vivo Evaluation as a Cosmeceutical Product

2021 ◽  
Vol 18 ◽  
Author(s):  
Nayla Javed ◽  
Shakeel Ijaz ◽  
Naveed Akhtar ◽  
Haji Muhammad Shoaib Khan
Keyword(s):  
Zeta Potential ◽  
Biological Activities ◽  
Skin Permeation ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Potential Candidate ◽  
Skin Rejuvenation ◽  
In Vivo Evaluation

Background: Arctostaphylos uva-ursi (AUU) being rich in polyphenols and arbutin is known to have promising biological activities and can be a potential candidate as a cosmaceutical. Ethosomes encourage the formation of lamellar-shaped vesicles with improved solubility and entrapment of many drugs including plant extracts. Objective: The objective of this work was to develop an optimized nanostructured ethosomal gel formulation loaded with AUU extract and evaluated for skin rejuvenation and depigmentation. Methods: AUU extract was tested for phenolic and flavonoid content, radical scavenging potential, reducing power activity, and in-vitro SPF (sun protection factor) estimation. AUU loaded 12 formulations were prepared and characterized by SEM (scanning electron microscopy), vesicular size, zeta potential, and entrapment efficiency (%EE). The optimized formulation was subjected to non-invasive in-vivo investigations after incorporating it into the gel system and ensuring its stability and skin permeation. Results: Ethosomal vesicles were spherical in shape and Zeta size, zeta potential, PDI (polydispersity index), % EE and in-vitro skin permeation of optimized formulation (F3) were found to be 114.7nm, -18.9mV, 0.492, 97.51±0.023%, and 79.88±0.013% respectively. AUU loaded ethosomal gel formulation was stable physicochemically and exhibited non-Newtonian behavior rheologically. Moreover, it significantly reduced skin erythema, melanin as well as sebum level and improved skin hydration and elasticity. Conclusion: A stable AUU based ethosomal gel formulation could be a better vehicle for phytoextracts than conventional formulations for cosmeceutical applications such as for skin rejuvenation and depigmentation etc.

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