Copro-PCR prevalence of Echinococcus granulosus infection in dogs in Kerman, south-eastern Iran

2017 ◽  
Vol 92 (1) ◽  
pp. 17-21 ◽  
Author(s):  
S.R. Mirbadie ◽  
H. Kamyabi ◽  
M.A. Mohammadi ◽  
S. Shamsaddini ◽  
M.F. Harandi
Keyword(s):  
Echinococcus Granulosus ◽  
Pcr Analysis ◽  
Stray Dogs ◽  
South Eastern ◽  
Concentration Method ◽  
Eastern Iran ◽  
Faecal Samples ◽  
Control Programmes ◽  
Polymerase Chain ◽  
The City

AbstractThe main objective of this study was to determine the prevalence of taeniid parasites and the specific detection of Echinococcus granulosus using copro-DNA polymerase chain reaction (PCR) analysis in the stray dogs of Kerman, south-eastern Iran. From September 2013 to May 2014, faecal samples of stray dogs were collected from different parts of the city of Kerman and its suburbs. Faecal samples from dogs were collected randomly within 24 h of defecation. All samples were transferred to the research lab and coprological examinations were conducted by the formalin–ether concentration method. In the microscopically positive samples, mitochondrial cytochrome c oxidase subunit 1 (cox1) specific primers were used to determine the taeniid identity of the infection. In addition, another set of primers was used for the specific diagnosis of E. granulosus sensu lato. In total, 307 faecal samples from stray dogs were examined for the presence of the parasites. Taeniidae eggs were detected in 34 dogs (11.07%). All 34 taeniid-positive specimens were PCR positive for cox1 (444 bp). Of all taeniid-positive specimens, 21 samples (6.8% of all dog specimens) were positive according to primers specific for E. granulosus. The findings of the present study revealed that canine echinococcosis is prevalent in the stray dogs in Kerman. The findings of the present study have important implications for hydatid control programmes in the area.

scholarly journals Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey

2018 ◽  
Vol 62 (4) ◽  
pp. 497-502 ◽  
Author(s):  
Bekir Oguz ◽  
Nalan Ozdal ◽  
Ozlem Orunc Kilinc ◽  
M. Serdar Deger
Keyword(s):  
Echinococcus Granulosus ◽  
Parasite Control ◽  
Larval Form ◽  
Stray Dogs ◽  
Concentration Method ◽  
Faecal Samples ◽  
Coprological Examination ◽  
Pcr Method ◽  
Different Parts ◽  
Control And Eradication

AbstractIntroduction:Echinococcus granulosus is a zoonotic helminth of the Taeniidae family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of E. granulosus in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of E. granulosus eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method.Material and Methods: This study was conducted between 2015 and 2016 on the faeces obtained from 100 stray dogs from different parts of the Van Province. The coprological examination was conducted using the formalin-ether concentration method.Results:Taeniidae eggs were found in 10 (10%) out of 100 faecal samples. E. granulosus was detected in 4 out of 10 of these (40%) infected samples. Sequence analysis of positive amplicons obtained from PCR showed that there were sheep strains (G1).Conclusion: Dogs in Van area are primarily infected with the livestock genotype of E. granulosus, which is thought to be a potential zoonotic threat to humans.

scholarly journals Opportunistic Mapping of Strongyloides stercoralis and Hookworm in Dogs in Remote Australian Communities

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 398
Author(s):  
Meruyert Beknazarova ◽  
Harriet Whiley ◽  
Rebecca Traub ◽  
Kirstin Ross
Keyword(s):  
One Health ◽  
Unknown Origin ◽  
Strongyloides Stercoralis ◽  
Pcr Analysis ◽  
Limited Information ◽  
Prevention Strategies ◽  
Remote Communities ◽  
Faecal Samples ◽  
Polymerase Chain ◽  
Insight Into

Both Strongyloides stercoralis and hookworms are common soil-transmitted helminths in remote Australian communities. In addition to infecting humans, S. stercoralis and some species of hookworms infect canids and therefore present both environmental and zoonotic sources of transmission to humans. Currently, there is limited information available on the prevalence of hookworms and S. stercoralis infections in dogs living in communities across the Northern Territory in Australia. In this study, 274 dog faecal samples and 11 faecal samples of unknown origin were collected from the environment and directly from animals across 27 remote communities in Northern and Central Australia. Samples were examined using real-time polymerase chain reaction (PCR) analysis for the presence of S. stercoralis and four hookworm species: Ancylostoma caninum, Ancylostoma ceylanicum, Ancylostoma braziliense and Uncinaria stenocephala. The prevalence of S. stercoralis in dogs was found to be 21.9% (60/274). A. caninum was the only hookworm detected in the dog samples, with a prevalence of 31.4% (86/274). This study provides an insight into the prevalence of S. stercoralis and hookworms in dogs and informs future intervention and prevention strategies aimed at controlling these parasites in both dogs and humans. A “One Health” approach is crucial for the prevention of these diseases in Australia.

scholarly journals AUTENTIKASI DAGING AYAM SEGAR DARI KONTAMINASI DAGING BABI MENGGUNAKAN GEN CYT-B DENGAN ANALISIS DUPLEX- POLYMERASE CHAIN REACTION

2017 ◽  
Vol 41 (2) ◽  
pp. 113
Author(s):  
Bayu Setya Hertanto ◽  
Rizky Aulia Fitra ◽  
Lilik Retna Kartikasari ◽  
Muhammad Cahyadi
Keyword(s):  
Meat Products ◽  
Chicken Meat ◽  
Pcr Analysis ◽  
Processed Meat ◽  
Duplex Pcr ◽  
Cyt B ◽  
Polymerase Chain ◽  
Contamination Levels ◽  
The City ◽  
Dna Isolation Protocol

Halal is one of important aspects in consumer protection. Meat and processed meat products are food that should be controlled strictly because those are prone to be adulterated by pork contamination. Therefore, it is necessary to provide detection technique which is accurate, fast and cheap. The objective of this research was to identify the presence of impurities of pork meat on raw chicken meat using gene Cyt-b with duplex-PCR analysis. This research used six samples of raw chicken meat and raw pork. Raw chicken meat was bought from supermarkets in the city of Surakarta and raw pork was obtained from pig slaughterhouse. The percentage of raw pork contamination on raw chicken meat was designed as much as 1, 5, 10, and 25%, respectively. The DNA genome was isolated according to DNA isolation protocol from Genomic DNA Mini Kit. In addition, duplex-PCR was performed based on protocol of KAPA2G Fast Multiplex PCR kit. The data was descriptively analyzed by directly looking the DNA bands on the gel documentation apparatus. The result showed that specific DNA bands for chicken and pig were completely appeared on 1.5% of agarose gels. Duplex-PCR detect contamination of pork on raw meat of chicken at all contamination levels. This research proved that the duplex-PCR detect the contamination of pork until the level of 1%.

Establishment of co-infection and hybridization of Haemonchus contortus and Haemonchus placei in sheep

2018 ◽  
Vol 93 (6) ◽  
pp. 697-703 ◽  
Author(s):  
M.C. Santos ◽  
M.R.V. Amarante ◽  
A.F.T. Amarante
Keyword(s):  
Haemonchus Contortus ◽  
First Generation ◽  
Pcr Analysis ◽  
Gold Standard Method ◽  
Morphological Evaluation ◽  
Faecal Samples ◽  
Polymerase Chain ◽  
Species Specific ◽  
Establishment Rate ◽  
Haemonchus Placei

AbstractThis study aimed to evaluate the simultaneous infections of Haemonchus contortus and Haemonchus placei in sheep, as well as the production of hybrids. A parental group of lambs (n = 6) were mix-infected with 2000 infective larvae (L3) of H. placei and 2000 L3 of H. contortus. Faecal samples were taken from each of these six lambs to produce the first generation of L3 (F1-L3) in individual cultures. These F1-L3 were used to infect 12 lambs; six of them were euthanized at 42 days (Group F1-42) and six at 84 days (Group F1-84) post infection. Polymerase chain reaction (PCR) analysis, using species-specific primer pairs, was the gold standard method for identification of Haemonchus adult species and hybrids. The establishment rate of both species was similar in the parental group: 51.7% H. contortus and 48.3% H. placei. Of the 219 adult specimens from groups F1-42 and F1-84 analysed by PCR, eight (3.65%) were hybrids, 111 were H. contortus and 100 were H. placei. The morphological evaluation of the F1-L3 from the parental group showed a predominance of larvae with H. contortus size (51.5%) in comparison with H. placei (42.8%). In the second generation of L3 (F2-L3) produced by the F1-lambs, larvae with H. contortus morphology predominated, with 81.5% in the F1-42 group and 84.0% in the F1-84 group. In conclusion, an artificial mixed infection by H. contortus and H. placei was established in lambs and resulted in the production of a small number of hybrids among their offspring.

Presence ofSalmonellaspp.,Yersinia enterocolitica,Yersinia pseudotuberculosisandEscherichia coliO157:H7 in wild boars

2014 ◽  
Vol 142 (12) ◽  
pp. 2542-2547 ◽  
Author(s):  
A. SANNÖ ◽  
A. ASPÁN ◽  
G. HESTVIK ◽  
M. JACOBSON
Keyword(s):  
Public Health ◽  
Wild Boar ◽  
Yersinia Enterocolitica ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Serious Illness ◽  
Wild Boars ◽  
European Wild Boar ◽  
Faecal Samples ◽  
Polymerase Chain

SUMMARYThe European wild boar populations are growing and spreading to new areas, which might constitute a threat to public health, since wild boar can harbour pathogens with the potential to cause serious illness in humans. Tonsils, ileocaecal lymph nodes and faecal samples were collected from 88 Swedish wild boars and analysed for the presence of the zoonotic pathogensSalmonellaspp.,Yersinia enterocolitica, Y. pseudotuberculosisand enterohaemorrhagicEscherichia coliO157:H7 (EHEC). A combination of cultivation and polymerase chain reaction (PCR) analysis was used and overall, 20% of sampled individuals tested positive forY. enterocolitica, 20% forY. pseudotuberculosisand 10% forSalmonellaspp. A total of 41% of sampled individuals tested positive for one or more of these three pathogens. No EHEC were detected. Samples PCR-positive forSalmonellaspp. were cultivated further and six isolates were obtained, belonging toSalmonella entericasubspeciesentericaand subspeciesdiarizone. The pathogens were most commonly detected in tonsil samples.

Molecular genotyping of Echinococcus granulosus from dromedaries (Camelus dromedarius) in eastern Iran

2013 ◽  
Vol 89 (1) ◽  
pp. 100-104 ◽  
Author(s):  
E. Moghaddas ◽  
H. Borji ◽  
A. Naghibi ◽  
P. Shayan ◽  
G.R. Razmi
Keyword(s):  
Echinococcus Granulosus ◽  
Rflp Analysis ◽  
Camelus Dromedarius ◽  
Public Health Importance ◽  
Morphological Findings ◽  
Eastern Iran ◽  
Morphological Criteria ◽  
Geographical Regions ◽  
Pcr Rflp ◽  
Polymerase Chain

AbstractWith the aim of genotyping Echinococcus granulosus cysts found in Iranian dromedaries (Camelus dromedarius), 50 cysts of E. granulosus were collected from five geographical regions in Iran. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1). Morphological criteria using rostellar hook dimensions were also undertaken. The present results have shown that 27 out of 50 E. granulosus cysts (54%) were determined as the G1 strain, and the other (46%) were determined as the G6 strain. The molecular analysis of the ITS1 region of ribosomal DNA corresponded with the morphological findings. Because of its recognized infectivity in humans, the G1 genotype is a direct threat to human health and its presence in Iranian dromedaries is of urgent public health importance.

Epidemiology of intestinal helminth parasites in stray dogs from markets in south-eastern Nigeria

2010 ◽  
Vol 85 (4) ◽  
pp. 415-420 ◽  
Author(s):  
I.C. Okoye ◽  
N.R. Obiezue ◽  
C.E. Okorie ◽  
I.E. Ofoezie
Keyword(s):  
Cost Effective ◽  
Effective Control ◽  
Intestinal Helminth ◽  
Helminth Parasites ◽  
Intensity Pattern ◽  
Stray Dogs ◽  
South Eastern ◽  
Gastrointestinal Helminth ◽  
Faecal Samples ◽  
Trichuris Vulpis

AbstractA survey of gastrointestinal helminth parasites of stray dogs (Canis familiaris) was conducted at Obollo-Afor and Ekwulobia markets, in Enugu and Anambra States, south-eastern Nigeria, respectively, to determine the patterns of infection among dogs in different parts of south-eastern Nigeria. Faecal samples collected, using long forceps, from every dog encountered in the markets between June 2007 and December 2008 were analysed by the Kato–Katz technique. Out of 413 dogs examined in both markets, 217 (52.6%) were infected with at least one of five parasites (Toxocara spp., Dipylidium caninum, Ancylostoma caninum, Taenia spp. and Trichuris vulpis). Overall faecal egg intensity of infection was 49.9 ± 58.7 eggs/g (epg). The prevalence of infection was comparable between the markets and between the male and female dogs, but varied significantly (P < 0.05) by age, decreasing from 78.9% in pups to 36.0% in adult dogs. The mean intensity pattern was similar to that of prevalence, decreasing from 86.7 ± 63.0 epg in pups to 22.1 ± 34.4 in adults. The most important individual parasite infection was Ancylostoma spp. (39.2%; 30.0 ± 41.2 epg) while T. vulpis was the least important (1.9%; 0.7 ± 5.4 epg). Generally, prevalence and intensity patterns of each parasite were also comparable between the markets and between sexes, but significantly (P < 0.05) age-dependent. The implications of these findings to public health in Nigeria and other endemic countries are discussed in relation to options for cost-effective control design and implementation.

Molecular identification of hookworm isolates from stray dogs, humans and selected wildlife from South Africa

2019 ◽  
Vol 94 ◽  
Author(s):  
P. I. Ngcamphalala ◽  
J. Lamb ◽  
S. Mukaratirwa
Keyword(s):  
South Africa ◽  
Polymerase Chain Reaction ◽  
Fragment Length Polymorphism ◽  
Domestic Animals ◽  
Chain Reaction ◽  
Stray Dogs ◽  
5.8S Rrna ◽  
Faecal Samples ◽  
Pcr Rflp ◽  
Polymerase Chain

Abstract There is a paucity of information on hookworm species in humans, domestic animals and wildlife in southern Africa. Our study aimed to identify hookworm species from stray dogs, humans, and selected wildlife from South Africa. A total of 356 faecal samples were screened for the presence of hookworm-like eggs and subsequently coproculture from the positive samples was carried out to obtain larvae. Hookworm-like eggs were detected in 23.03% (82/356) of samples. Of these samples, 78/296 were from dogs, 3/50 from humans and 1/10 from wildlife. DNA was then isolated from the larvae of 55 positive samples, which were subjected to polymerase chain reaction (PCR), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing of the nuclear ribosomal internal transcribed spacer (ITS1) and 5.8S rRNA region. Presence of Ancylostoma caninum, A. braziliense and A. ceylanicum-like species was recorded in stray dogs and A. caninum was recorded in wildlife and humans, using PCR-RFLP. Although PCR-RFLP results pointed to the presence of A. ceylanicum, we did not get a sequence that matched with A. ceylanicum from GenBank. This may have been due to the low proportion of A. ceylanicum larvae in our samples. Twenty-two of the 27 positive amplicons from stray dogs matched with A. caninum, three with A. braziliense and two had mixed infections of A. braziliense and A. caninum. Sequences from a lion and three humans matched with A. caninum. This is the first confirmation of a patent A. caninum infection in humans as evidenced by the presence of eggs in faeces, with the subsequent larvae from coproculture being identified as A. caninum.

scholarly journals Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran

2019 ◽  
Author(s):  
Fariba BERENJI ◽  
Seyed Aliakbar SHAMSIAN ◽  
Marziyeh NOURI DALOEE ◽  
Seyed Hossein FATTAHI MASOOM ◽  
Elham MOGHADDAS
Keyword(s):  
Echinococcus Granulosus ◽  
Nadh Dehydrogenase ◽  
Rflp Analysis ◽  
Medical Sciences ◽  
Lung Cysts ◽  
Eastern Iran ◽  
Pcr Rflp ◽  
North Eastern ◽  
Polymerase Chain ◽  
Sheep Strain

Background: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically. Methods: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1). Results: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified. Conclusion: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.

Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype

2011 ◽  
Vol 86 (3) ◽  
pp. 263-270 ◽  
Author(s):  
E. Hajialilo ◽  
M.F. Harandi ◽  
M. Sharbatkhori ◽  
H. Mirhendi ◽  
S. Rostami
Keyword(s):  
Echinococcus Granulosus ◽  
Human Isolate ◽  
Sequencing Data ◽  
Intermediate Hosts ◽  
Eastern Iran ◽  
East Of Iran ◽  
Animal Hosts ◽  
Polymerase Chain ◽  
Cattle And Sheep

AbstractEchinococcus granulosus, the aetiologic agent of cystic echinococcosis (CE), is one of the most important zoonotic helminthes worldwide. Isolates of the parasite show considerable genetic variation in different intermediate hosts. Several genotypes and species are described in different eco-epidemiological settings. This study investigated E. granulosus genotypes existing in livestock and humans from the province of Kerman, located in south-eastern Iran, using sequencing data of cox1 and nad1 mitochondrial genes. Fifty-eight E. granulosus isolates, including 35 from sheep, 11 from cattle, 9 from camels and 3 from goats, were collected from slaughterhouses throughout Kerman. One human isolate was obtained from a surgical case of CE. Mitochondrial cox1 and nad1 regions were amplified using polymerase chain reaction (PCR) and 38 isolates were sequenced. Genotypes G1 (73.7%), G3 (13.2%) and G6 (13.1%) were identified from the isolates. G1 was the most common genotype from sheep (86.7%), cattle (80%), camels (44.4%) and goats (100%). Sheep, cattle and camels were also found to be infected with the G3 genotype (buffalo strain). The human isolate was identified as the G6 genotype. Results showed that the G3 genotype occurred in different animal hosts in addition to G1 and G6 genotypes.

Sign in / Sign up

Export Citation Format

Share Document