scholarly journals Epidemic Diarrhoea and the Bacterial Content of Food

1906 ◽  
Vol 6 (1) ◽  
pp. 77-92 ◽  
Author(s):  
J. E. Sandilands
Keyword(s):  
Complex Medium ◽  
General Recognition ◽  
Bacterial Content

Since it would hardly be possible to conceive a more complex medium for the conveyance of disease than cows' milk, the solution of the problems connected with the origin and source of epidemic diarrhoea is not materially advanced by the general recognition of the fact that in the majority of fatal cases, cows' milk is the vehicle of infection.

THEORIES OF THE MISSIONARY VOCATION OF THEOLOGICAL EDUCATION IN POST-SOVIET PROTESTANTISM

2020 ◽  
pp. 78-92
Author(s):  
Valentyn Syniy
Keyword(s):  
21St Century ◽  
Theological Education ◽  
Educational Activity ◽  
Spiritual Education ◽  
General Recognition ◽  
The Church ◽  
The Trinity ◽  
Formal Construction

It is emphasized that the involvement of missionary theology in the discussion of ways to develop spiritual education allowed post-soviet Protestantism to successfully overcome differences in the vision of the formal construction of education, and then move on to discussions about its content. There was a gradual overcoming of modern individualism, the growing role of communities, the replacement of monologue models of mission with dialogical ones. The idea of the seminary as a community that is not self-sufficient, but serves the church as a community, has gained general recognition. The church also came to be understood as serving an eschatological ideal community similar to the Trinity community. The formation of community and dialogical models of missionary and educational activity allows Ukrainian Protestantism to effectively adapt to the realities of the beginning of the 21st century and to be proactive in today's society.

BACTERIAL CONTENT OF DRINKING WATER IN THE SAGARMATHA NATIONAL PARK, NEPAL

2016 ◽  
Author(s):  
Kirsten N. Nicholson ◽  
◽  
Klaus Neumann ◽  
Carolyn B. Dowling ◽  
Subodh Sharma
Keyword(s):  
Drinking Water ◽  
National Park ◽  
Sagarmatha National Park ◽  
Bacterial Content

scholarly journals Genetic analysis of temperature-sensitive lethal mutants of Salmonella typhimurium.

Genetics ◽  
1989 ◽  
Vol 123 (4) ◽  
pp. 625-633 ◽  
Author(s):  
M B Schmid ◽  
N Kapur ◽  
D R Isaacson ◽  
P Lindroos ◽  
C Sharpe
Keyword(s):  
High Temperature ◽  
Genetic Analysis ◽  
Salmonella Typhimurium ◽  
Complex Medium ◽  
Temperature Sensitive ◽  
Nonpermissive Temperature ◽  
Anaerobic Conditions ◽  
Temperature Growth ◽  
Lethal Phenotype ◽  
Lethal Mutations

Abstract We have isolated 440 mutants of Salmonella typhimurium that show temperature-sensitive growth on complex medium at 44 degrees. Approximately 16% of the mutations in these strains have been mapped to 17 chromosomal locations; two of these chromosomal locations seem to include several essential genes. Genetic analysis of the mutations suggests that the collection saturates the genes readily mutable to a ts lethal phenotype in S. typhimurium. Physiological characteristics of the ts lethal mutants were tested: 6% of the mutants can grow at high temperature under anaerobic conditions, 17% can grow when the medium includes 0.5 M KCl, and 9% of the mutants die after a 2-hr incubation at the nonpermissive temperature. Most ts lethal mutations in this collection probably affect genes required for growth at all temperatures (not merely during high temperature growth) since Tn10 insertions that cause a temperature-sensitive lethal phenotype are rare.

scholarly journals Dose-rate effect of low-energy electron beam irradiation on bacterial content in chilled turkey

2021 ◽  
Vol 640 (3) ◽  
pp. 032006
Author(s):  
U A Bliznyuk ◽  
P Yu Borchegovskaya ◽  
A P Chernyaev ◽  
V S Ipatova ◽  
V A Leontiev ◽  
...  
Keyword(s):  
Electron Beam ◽  
Dose Rate ◽  
Electron Beam Irradiation ◽  
Rate Effect ◽  
Low Energy ◽  
Energy Electron ◽  
Beam Irradiation ◽  
Low Energy Electron ◽  
Bacterial Content ◽  
Dose Rate Effect

scholarly journals The Atmosphere of the Underground Electric Railways of London: A Study of its Bacterial Content in 1920

1923 ◽  
Vol 22 (2) ◽  
pp. 123-155 ◽  
Author(s):  
J. Graham Forbes
Keyword(s):  
Body Temperature ◽  
Room Temperature ◽  
The Mean ◽  
Bacterial Content ◽  
The City

1. The bacterial content of the air of the Underground Railways, when the average of all results of the bacteriological investigations is taken, does not numerically compare unfavourably with the outside air of London.2. The ratio of the number of organisms growing at room temperature appears to be about 14 for railway air to 10 outside air. For those growing at body temperature the ratio is considerably higher, namely 2 to 1 respectively. The mean per litre, for room temperature organisms, is about 9 in railway air, 6·3 in the outside air; for body temperature organisms 4·6 for railway air, 2·2 for outside air.3. The bacterial content of platform air, except on the City and South London Railway, would appear to be higher than that of carriage air; the total mean for platform air being 52 and for carriage air 42·8 organisms per 5 litres, or a ratio of 16·4 and 13·5 respectively to 10 of the open air. The higher proportion in platform air is generally speaking to be accounted for by the greater amount of draught and dust disturbance.4. The ratios of the total bacterial content of railway carriage air and carriage and platform air on the six lines to open air are estimated in the following proportions:

scholarly journals Selected Alternative Feed Additives Used to Manipulate the Rumen Microbiome

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1542
Author(s):  
Marta Michalak ◽  
Konrad Wojnarowski ◽  
Paulina Cholewińska ◽  
Natalia Szeligowska ◽  
Marcel Bawej ◽  
...  
Keyword(s):  
Digestive System ◽  
Animal Husbandry ◽  
Review Article ◽  
Feed Additives ◽  
Microbial Fermentation ◽  
Plant Origin ◽  
Gelidium Amansii ◽  
Alternative Feed ◽  
Rumen Microbiome ◽  
General Recognition

In recent years, a boost in the ruminant population has been observed, and consequently, an increase in the animals’ demand for nutrients and methane emissions. Methane emission is generated during the microbial fermentation of feed in the rumen, and a percentage even up to 12% of the energy obtained by this process can be wasted. In addition, the use of antibiotics in animal husbandry is being increasingly restricted. restricted. As a result, there is a continuous search for innovative feed additives that can serve as alternatives to antibiotics, and will also be safe for both people and the environment. In the present review article, additives were selected on basis that, according to studies conducted so far, may positively affect the microbiome of the digestive system by improving indicators and/or reducing methane production. Among them, probiotics, prebiotics or their combination—synbiotics are at the forefront of research. However, additives in the form of algae or plant origin are also gaining ground in popularity, such as essential oils, fermented wheat straw or Gelidium amansii, due to their general recognition as safe (GRAS) for both humans and environment.

Preparation of a highly active form of nisin from Streptococcus lactis

1971 ◽  
Vol 17 (1) ◽  
pp. 61-67 ◽  
Author(s):  
F. J. Bailey ◽  
A. Hurst
Keyword(s):  
Active Form ◽  
Gradient Elution ◽  
Antibiotic Activity ◽  
Complex Medium ◽  
Main Peak ◽  
Acetone Precipitation ◽  
Highly Active ◽  
Streptococcus Lactis ◽  
Constant Ph ◽  
Ph Gradient Elution

Cells of Streptococcus lactis (354/07) synthesized and retained nisin when grown in a complex medium with 2.5% glucose at a constant pH of 6.7. Nisin was extracted from cells by a previously used method with hot 0.05 N HCl but milder methods of extraction from whole and broken cells using a variety of solvents were also tested. In the preferred method broken cells were extracted with 0.05 N HCl at 2 °C. The Cl− ions of the extract were exchanged for acetate on columns of the resin Amberlite CG 4B and the eluate was concentrated by acetone precipitation at −19 °C. The nisin was finally purified by pH gradient elution from CM cellulose columns. Three peaks with antibiotic activity were found, two of the peaks were minor and represented less than 5% of the nisin. The main peak gave a single band on electrophoresis. Electrophoresis of the material from the CM cellulose peaks revealed about 44 bands of basic proteins. Nisin made by the hot or cold HCl extraction behaved similarly in electrophoresis and CM cellulose chromatography but the antibiotic activity of the material isolated from the cold extract was nine times greater than that of the material isolated from the hot extract.

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