Effect of local use of l-carnitine after myringotomy on myringosclerosis development in rats

2013 ◽  
Vol 127 (5) ◽  
pp. 468-472 ◽  
Author(s):  
E Vuralkan ◽  
S Alicura Tokgöz ◽  
G Simsek ◽  
F Koybasioglu ◽  
U Han ◽  
...  
Keyword(s):  
Tympanic Membrane ◽  
Intraperitoneal Administration ◽  
Membrane Thickness ◽  
Fibroblast Proliferation ◽  
Experimental Setting ◽  
Treatment Efficiency ◽  
Limited Effectiveness ◽  
Local Use ◽  
Fibroblastic Proliferation ◽  
Tympanic Membranes

AbstractObjectives:This study aimed to investigate the effect of local and intraperitoneal administration of l-carnitine on the prevention of experimentally induced myringosclerosis, and to compare treatment efficiency.Methods:Twenty-four Albino-Wistar rats (48 ears) were bilaterally myringotomised and divided randomly into four groups: group one received no treatment, group two received intraperitoneal l-carnitine, group three received local l-carnitine, and group four received both intraperitoneal and local l-carnitine. On the 15th day after treatment, tympanic membranes were harvested and evaluated histopathologically for myringosclerotic plaque formation, fibroblastic proliferation, tympanic membrane thickness and new vessel formation.Results:The group one tympanic membranes showed extensive thickness, and the incidence of myringosclerosis and fibroblast proliferation were greater than in groups two and four. There were statistically significant differences in tympanic membrane thickness between groups three and four, and in myringosclerosis incidence and fibroblast proliferation, comparing groups two, three and four.Conclusion:Myringosclerosis development was significantly reduced in rats receiving myringotomy plus intraperitoneal l-carnitine. Intraperitoneal l-carnitine administration prevented fibroblastic proliferation and tympanic membrane thickening (both of which cause further tympanic membrane destruction), thus reducing myringotomy-associated morbidity. Local l-carnitine administration had limited effectiveness in this experimental setting.

scholarly journals Recovery from tympanic membrane perforation: Effects on membrane thickness, auditory thresholds, and middle ear transmission

2019 ◽  
Vol 384 ◽  
pp. 107813 ◽  
Author(s):  
Lingling Cai ◽  
Glenna Stomackin ◽  
Nicholas M. Perez ◽  
Xiaohui Lin ◽  
Timothy T. Jung ◽  
...  
Keyword(s):  
Tympanic Membrane ◽  
Middle Ear ◽  
Tympanic Membrane Perforation ◽  
Membrane Thickness ◽  
Auditory Thresholds ◽  
Membrane Perforation

Experimental Myringoplasty

1980 ◽  
Vol 3 (6) ◽  
pp. 354-357 ◽  
Author(s):  
L. Feenstra ◽  
B.W.C. Van Der Ven ◽  
F.E. Kohn ◽  
J. Feijen
Keyword(s):  
Lactic Acid ◽  
Bisphenol A ◽  
Tympanic Membrane ◽  
Biodegradable Polymers ◽  
Glycolic Acid ◽  
Tissue Reaction ◽  
Biodegradable Poly ◽  
One Year ◽  
Poly Carbonate ◽  
Tympanic Membranes

Artificial eardrums made from biodegradable poly(D, L-lactic acid), poly(glycolic acid) and poly(ß-benzyl-L-aspartate-co-L-leucine) 50/50, and made from the microporous poly(tetrafluoroethylene) and bisphenol-A poly(carbonate) membranes were implanted into the ear and as a reference subcutaneously in rats. The implants were histologically examined for periods up to one year. From the biodegradable polymers studied the poly(ß-benzyl-L-aspartate-co-L-leucine) 50/50 evoked the least tissue reaction and the newly formed tympanic membranes are the best in terms of thickness and overall integrity. The microporous poly(tetrafluoroethylene) membrane can be considered as a valuable support for the formation of a reinforced tympanic membrane.

Development and Evaluation of an Objective Tympanic Membrane Visualization Assessment Technique

2020 ◽  
Vol 129 (8) ◽  
pp. 767-771 ◽  
Author(s):  
Amir A. Hakimi ◽  
Simon P. Lalehzarian ◽  
Aaron S. Lalehzarian ◽  
Ariel M. Azhdam ◽  
Benjamin D. Boodaie ◽  
...  
Keyword(s):  
Medical Students ◽  
Tympanic Membrane ◽  
Low Cost ◽  
Training Session ◽  
Standardized Patients ◽  
First Year ◽  
First Year Students ◽  
Before And After ◽  
Cohort Groups ◽  
Tympanic Membranes

Objectives: To introduce an inexpensive method for objectively evaluating otoscopic visualization of the tympanic membrane wherein learners match what they visualize in standardized patients to tympanic membrane photographs. Methods: Two standardized patients had photographs taken of their tympanic membranes using a commercially-available digital otoscope. First- and third-year medical students were asked to perform an otoscopic examination on each patient using a conventional handheld otoscope and to match what they saw with the correct tympanic membrane image among distractor photographs belonging to other patients. The ability of students to match the standardized patients’ tympanic membrane to the correct photographs was assessed before and after a didactic training session. These measurements were compared between the two cohort groups for construct validity. Results: Fifty-one first-year medical students (with no previous otoscopy experience) and 44 third-year medical students (with otoscopy experience from completing pediatric and family medicine clinical clerkships) were recruited to voluntarily participate in this study. At baseline, a larger percentage of third-year students correctly matched both tympanic membranes compared to first-year students (27% vs 8%, P < .01). After otoscopy training, correct matching of both tympanic membranes significantly improved among both first-year students (8-31%, P < .01) and third-year students (27-54%, P < .01). Conclusion: The use of tympanic membrane photographs from standardized patients provides a novel technique for objectively assessing proficiency in otoscopic visualization of the middle ear. The concept is low cost, uses live patients, and can be easily implemented in pre-clinical instruction and beyond.

Dendritic Cells in the Normal Human Tympanic Membrane

1995 ◽  
Vol 104 (10) ◽  
pp. 803-807 ◽  
Author(s):  
Burkhard Hussl ◽  
Gisela Egg ◽  
Weija Kong ◽  
Nikolaus Romani ◽  
Annelies Schrott-Fischer
Keyword(s):  
Dendritic Cells ◽  
Tympanic Membrane ◽  
Ultrastructural Level ◽  
Dc System ◽  
Birbeck Granules ◽  
Hla Dr ◽  
Positive Immunostaining ◽  
Tympanic Membranes

Dendritic cells (DCs) are antigen-presenting cells that possess an outstanding capacity to initiate primary immune responses. They reside in the tissues in an immunologically immature state. Upon antigenic challenge in vivo or short-term culture in vitro, they undergo a maturation process and turn into mature “lymphoid DCs.” Langerhans cells (LCs) of the epidermis were identified as members of this DC system. They have been demonstrated in cholesteatoma matrix and in inflamed tympanic membranes, but the normal tympanic membrane was hitherto thought to be devoid of them. To clarify this question, we removed 12 normal tympanic membranes postmortem and processed them for a sheet preparation. The epidermal layers were peeled off and immunostained with the following monoclonal antibodies: HLA-DR, OKT6/CD1 a, and LAG (specific for the Birbeck granules of LCs). Two tympanic membranes were also processed for routine electron microscopy. In all epidermal sheets a dense network of DCs could be demonstrated. They showed a positive immunostaining reaction with HLA-DR, but a negative one with OKT6 and LAG. Thus, they differ in their immunohistochemical properties from typical epidermal LCs. At the ultrastructural level, DCs could also be identified, but without the typical Birbeck granules. This explains the negative reaction with the LAG antibody. These findings were extended and supported by a tissue culture examination of three normal tympanic membranes. After 3 days, typical “veiled” cells (ie, mature DCs), showing positive immunostaining with HLA-DR, could be recovered from the culture medium. In an oxidative mitogenesis assay, these cells displayed strong stimulatory capacity for resting T lymphocytes. The presence of DCs in the normal tympanic membrane is an important clue for a better understanding of the immune status of the middle ear.

scholarly journals Tympanic membrane changes in experimental acute otitis media and myringotomy

Medicina ◽  
2008 ◽  
Vol 44 (4) ◽  
pp. 313 ◽  
Author(s):  
Giedrė Alzbutienė ◽  
Ann Hermansson ◽  
Per Cayè-Thomasen ◽  
Vytenis Kinduris
Keyword(s):  
Calcium Phosphate ◽  
Haemophilus Influenzae ◽  
Tympanic Membrane ◽  
Otitis Media ◽  
Acute Otitis Media ◽  
Polymorphonuclear Neutrophil ◽  
Sprague Dawley ◽  
Nontypeable Haemophilus Influenzae ◽  
Temporal Bones ◽  
Tympanic Membranes

Objective. The present experimental study explored pathomorphological changes and calcium depositions in the tympanic membrane during experimental acute otitis media caused by nontypeable Haemophilus influenzae in myringotomized and nonmyringotomized ears. Material and methods. A rat model of experimental acute otitis media caused by nontypeable Haemophilus influenzae was employed. Sixteen Sprague-Dawley rats were used. Four days following middle ear inoculation, a bilateral myringotomy was performed in six randomly selected animals. Another group of 10 animals was inoculated only. On days 4, 7, 14, and 28 after inoculation, two animals from each group were sacrificed. The temporal bones were removed and the tympanic membranes were dissected, followed by paraffin embedding. Adjacent sections were stained with PAS-alcian blue for basic histopathological observations and by von Kossa method for determination of calcium phosphate depositions. Results. Particularly intense invasion of polymorphonuclear neutrophil leukocytes was seen on day 4 after inoculation. The highest infiltration of macrophages was observed on day 7. The peak number of lymphocytes was seen on day 14. No difference occurred in the number of polymorphonuclear leukocytes in myringotomized and nonmyringotomized tympanic membranes. The infiltration with lymphocytes and activated macrophages in all parts of the myringotomized tympanic membranes was statistically significantly higher than in the nonmyringotomized animals. The total amount of interstitial calcium phosphate depositions during days 7, 14, and 28 of study was statistically higher in the sections of pars tensa from myringotomized membranes compared to the nonmyringotomized membranes. Conclusion. Nontypeable Haemophilus influenzae-induced acute otitis media and myringotomy provoke more extensive inflammatory reaction with microcalcification in the tympanic membranes.

Tympanic membrane thickness in mucoid otitis media

1999 ◽  
Vol 121 (2_suppl) ◽  
pp. P258-P258
Author(s):  
Muhammet Tekin ◽  
Cemil Mutlu ◽  
Michael M Paprella ◽  
Patricia A Schachern ◽  
Chap T Le
Keyword(s):  
Tympanic Membrane ◽  
Otitis Media ◽  
Membrane Thickness

Epithelial migration in the atelectatic tympanic membrane

2009 ◽  
Vol 123 (12) ◽  
pp. 1321-1324 ◽  
Author(s):  
I P Tang ◽  
N Prepageran ◽  
R Raman ◽  
T Sharizhal
Keyword(s):  
Methylene Blue ◽  
Tympanic Membrane ◽  
Migration Rate ◽  
Blue Staining ◽  
Blue Dye ◽  
Retraction Pocket ◽  
Epithelial Migration ◽  
Significant Difference ◽  
And Control ◽  
Tympanic Membranes

AbstractObjective:To determine whether epithelial migration in the atelectatic tympanic membrane (secondary to any pathology) occurs in a similar fashion to that in the normal (non-pathological) tympanic membrane, by calculating and comparing the epithelial migration rate and pattern.Study design:Prospective, non-randomised, case–control study. This study was a pilot study. We included patients with an atelectatic pars tensa region of the tympanic membrane and a healthy contralateral tympanic membrane (the latter used as the control).Setting:Otorhinolaryngology out-patient clinic. The study was conducted from January 2006 to January 2008.Intervention:A total of 40 patients (80 ears) were included based on their otoscopic appearance and tympanography findings. All patients' ears were examined under an operating microscope. A dot of methylene blue dye was applied in the immediate vicinity of the umbo of the atelectatic tympanic membrane, and in the same position on the tympanic membrane of the control ear. Patients were then followed up weekly until the dye dot had migrated to reach the annulus.Main outcome measures:Direction and rate of epithelial migration.Results:In the atelectatic tympanic membranes, epithelial migration proceeded from the centre of the retraction pocket toward the annulus in a lateral, radiating manner. Thirty-three (82.5 per cent) of the 40 patients showed a similar migratory pattern in both the study and control ears. Sixty per cent of the retracted tympanic membranes showed no migration in the first week after methylene blue staining. After the first week, the migration rate was nearly constant from the umbo towards the periphery, in both the study and control ears. The mean daily epithelial migration rate in the study and control ears was 62.6 and 64.7 µm/day, respectively; however, this difference was statistically insignificant (p = 0.202, independent samplest-test).Conclusion:There is no difference in the epithelial migration rate or pattern, comparing atelectatic tympanic membranes and normal tympanic membranes. However, there may be a significant difference in the epithelial migration rate with increased grades of tympanic membrane atelectasis.

Platelet-rich plasma improves healing of tympanic membrane perforations: experimental study

2008 ◽  
Vol 123 (5) ◽  
pp. 482-487 ◽  
Author(s):  
E Erkilet ◽  
M Koyuncu ◽  
S Atmaca ◽  
M Yarim
Keyword(s):  
Tympanic Membrane ◽  
Histopathological Examination ◽  
Platelet Rich Plasma ◽  
Healing Time ◽  
Tympanic Membrane Perforation ◽  
Control Group ◽  
Tympanic Membrane Perforations ◽  
Significant Difference ◽  
Membrane Perforation ◽  
Tympanic Membranes

AbstractObjective:The aim of this study was to investigate the effect of local application of platelet-rich plasma to perforated rat tympanic membranes, in terms of healing time and histopathological outcome.Methods:Eighty-eight tympanic membranes of 44 rats were given a standard 3 mm perforation, and platelet-rich plasma was applied to the right tympanic membrane perforations. The left tympanic membranes were left to heal spontaneously, as controls. The 44 rats were divided into two groups. In group one, comprising 20 rats, daily otomicroscopic examination of the tympanic membrane perforations was performed. The 24 rats in group two were subdivided into four subgroups of six rats each; these subgroups were sacrificed sequentially on days three, seven, 14 and 28 for histopathological examination, regardless of tympanic membrane healing stage.Results:In group one, the mean tympanic membrane healing times for tympanic membrane perforations receiving platelet-rich plasma and controls were respectively 10.2 ± 2.1 and 13.0 ± 2.9 days (mean ± standard deviation). This difference was statistically significant (p < 0.001). In group two, histopathological evaluation of tympanic membrane perforation healing at days three, seven, 14 and 28 did not reveal any statistically significant difference, individually or within the four groups as a whole.Conclusion:These findings suggest that earlier healing of tympanic membrane perforations occurred in the platelet-rich plasma group compared with the control group. These findings suggest that platelet-rich plasma is effective in accelerating tympanic membrane perforation healing, and that it may be effective in human subjects, particularly as it is an autologous material.

A Calibration Procedure for the Assessment of Thresholds above 8000 Hz

1982 ◽  
Vol 25 (4) ◽  
pp. 618-623 ◽  
Author(s):  
Patricia G. Stelmacttowicz ◽  
Michael P. Gorga ◽  
John K. Cullen
Keyword(s):  
Frequency Response ◽  
Flat Plate ◽  
Tympanic Membrane ◽  
Sound Pressure Level ◽  
Potential Application ◽  
Sound Pressure ◽  
Calibration Procedure ◽  
Pressure Level ◽  
Frequency Dependent ◽  
Tympanic Membranes

A technique is described to estimate the sound pressure level developed by a broad frequency response transducer at the tympanic membrane. Real-ear probe tube measurements near the tympanic membranes of 10 subjects were used to obtain frequency-dependent correction values for a custom-designed flat-plate coupler. These latter measures can be used tot routine calibration of the transducer. Audiometric thresholds from 250 to 16000 Hz were obtained on 14 children (5–18 years).Threshold estimates were found to be comparable to previouslv reported values. Potential application and limitations of this technique are discussed.

Comparative Histology of the Tympanic Membrane and its Relationship to Cholesteatoma

1989 ◽  
Vol 98 (10) ◽  
pp. 761-766 ◽  
Author(s):  
Richard A. Chole ◽  
Kevin Kodama
Keyword(s):  
Mast Cells ◽  
Tympanic Membrane ◽  
Lamina Propria ◽  
Guinea Pigs ◽  
Pars Flaccida ◽  
Pars Tensa ◽  
Retraction Pockets ◽  
Comparative Histology ◽  
Tympanic Membranes

The purpose of this study was to determine whether anatomic differences in the tympanic membranes of various species could explain differences in the propensity to form aural cholesteatomas and retraction pockets. Tympanic membranes from humans, dogs, cats, rabbits, guinea pigs, rats, gerbils, and mice were examined histologically. The pars flaccida and pars tensa varied greatly among the species studied. The guinea pig's pars flaccida was very small and had a thin lamina propria. In contrast, the lamina propria of the rabbit and cat pars flaccida were thick. The amount of collagen, elastin, mast cells, and macrophages varied widely. The human and gerbilline tympanic membranes were anatomically dissimilar; for example, the human pars flaccida and pars tensa contained more and denser collagen than did those of the gerbil. The presence of macrophages or mast cells did not correlate with the propensity to develop cholesteatomas. Therefore, anatomic differences among these species do not explain why some develop aural cholesteatomas and others do not.

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