Larval morphologies and potential developmental modes of eight sea spider species (Arthropoda: Pycnogonida) from the southern Oregon coast

2010 ◽  
Vol 91 (4) ◽  
pp. 845-855 ◽  
Author(s):  
Z.P. Burris
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Characteristics ◽  
Postembryonic Development ◽  
Oregon Coast ◽  
Spider Species ◽  
Sea Spider ◽  
Mode Of Development ◽  
First Time ◽  
Scanning Electron

Larvae of eight sea spiders from three families (Ammotheidae, Pycnogonidae and Phoxichilidiidae) are described for the first time. The external morphology of the first larval stage of each species is presented in detail using scanning electron microscopy photographs in order to determine the mode of postembryonic development. Three types of larval development are apparent in the species examined. The speciesAchelia gracilipes, Eurycyde spinosa, Pycnogonum rickettsiandPycnogonum stearnsi(families Ammotheidae and Pycnogonidae) have larval morphologies indicative of an ‘ectoparasitic’ development. Morphological characteristics ofAchelia simplissimaandAchelia chelata(family Ammotheidae) larvae suggest an ‘endoparasitic’ development, while larvae of the speciesAnoplodactylus viridintestinalis(family Phoxichilidiidae) have traits implying an ‘encysting’ postembryonic mode of development. Larvae of the speciesNymphopsis spinosissimahave unusual morphological characteristics that may be indicative of a new developmental mode.

scholarly journals Morphology of pollen in Ferula genus (Apiaceae)

PhytoKeys ◽  
2021 ◽  
Vol 179 ◽  
pp. 111-128
Author(s):  
Birol Başer ◽  
Mehmet Sagıroglu ◽  
Gulden Dogan ◽  
Hayri Duman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Morphological Characteristics ◽  
Pollen Grains ◽  
Polar Axis ◽  
Average Linkage ◽  
Pollen Shape ◽  
First Time ◽  
Scanning Electron

In this study, the pollen morphology of all Ferula species distributed throughout the country of Turkey was studied with light and scanning electron microscopy for the first time. The aim is to identify the pollen morphological characteristics of 23 Ferula species. The pollen is radially symmetrical, isopolar and tricolporate in all examined species. Pollen grains are prolate and perprolate with the polar axis ranging from 22.28 to 40.47 µm and the equatorial axis from 13.70 to 18.73 µm. Their polar shapes are triangular, triangular to subtriangular and circular to subcircular. Several types of exine ornamentations have been observed on pollen through the use of scanning electron microscopy. The dendrogram constructed by using Average Linkage of the examined data revealed two main groups. It was determined that some pollen characteristics are more useful for classification than others. In particular, P, E, the ratio of P/E (pollen shape) and ornamentation in the polar and equatorial views are the most valuable variables for discrimination the Ferula species.

Morphology of the first zoeal stage of the Mediterranean bumblebee shrimpGnathophyllum elegansstudied with both light microscopy and scanning EM

2013 ◽  
Vol 94 (1) ◽  
pp. 151-158 ◽  
Author(s):  
Roland Meyer ◽  
Tobias Lehmann ◽  
Roland R. Melzer ◽  
Hannes Geiselbrecht
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Differential Diagnosis ◽  
Morphological Characteristics ◽  
Zoeal Stage ◽  
High Similarity ◽  
Systematic Relationships ◽  
Scanning Em ◽  
First Time ◽  
Scanning Electron

The morphology of the first zoea ofGnathophyllum elegansis described from laboratory-reared material for the first time and analysed in detail with light and scanning electron microscopy. For systematic reasons, morphological characteristics inG. elegansare compared with those inGnathophyllum americanum, Periclimenes amethysteus, a representative of the subfamily Pontoniinae and the closely relatedHymenocera picta(Decapoda: Caridea: Hymenoceridae). We observed differences in the morphology of bothGnathophyllum-larvae, such as the number and arrangement of certain setae. Thus, larvae of the twoGnathophyllumspecies can be readily distinguished from each other. Further differential diagnosis withP. amethysteusconfirms a high similarity betweenGnathophyllum-larvae and larvae in the subfamily Pontoniinae, as already mentioned in earlier publications. The systematic relationships of the Gnathophyllidae, Hymenoceridae and Pontoniinae are discussed based on zoeal characters.

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

scholarly journals Labellum and gynostemium micromorphology in Polystachya (Orchidaceae)

2020 ◽  
Vol 307 (1) ◽  
Author(s):  
Mytnik Joanna ◽  
Davies L. Kevin ◽  
Narajczyk Magdalena ◽  
Łuszczek Dorota ◽  
Kubiak Joanna ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sub Saharan Africa ◽  
Capitate Trichomes ◽  
Sub Saharan ◽  
First Time ◽  
Scanning Electron

AbstractPolystachya is a large, pantropical orchid genus of 200 species, most of which occur as epiphytes in sub-saharan Africa. The three-lobed labellum of most Polystachya species possesses a fleshy callus and various types of trichomes and papillae. In this paper, we present the results of micromorphological studies on the labellum of 20 species, representing eight of the 13 sections in the genus, using scanning electron microscopy (SEM). Our results show the scale of infrageneric diversity of trichomes and papillae relative to the sampled sections. They also demonstrate the taxonomic value of labellar micromorphology at the sectional level. The study revealed seven types of papillae and five types of trichomes (uni- and multi-cellular) in Polystachya, some of which, are described here for the first time. Clavate trichomes are the most common and are present in 60% of the species studied. Moniliform trichomes mainly occur in sect. Polystachya and are strongly characteristic of the section. Pseudopollen is formed by fragmentation of moniliform trichomes or the detachment of other trichomes as bicellular units. We provide, for the first time, evidence for the detachment of the terminal cells of capitate trichomes.

Scanning Electron Microscopy Coupled with Energy Dispersive Spectrometric Analysis Reveals for the First Time Weddellite and Sylvite Crystals on the Surface of Involucral Bracts and Petals of two Xeranthemum L. (Compositae) Species

2017 ◽  
Vol 23 (3) ◽  
pp. 679-686 ◽  
Author(s):  
Milan Gavrilović ◽  
Suzana Erić ◽  
Petar D. Marin ◽  
Núria Garcia-Jacas ◽  
Alfonso Susanna ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glandular Trichomes ◽  
Energy Dispersive ◽  
Spectrometric Analysis ◽  
Eds Analysis ◽  
Taxonomic Studies ◽  
First Time ◽  
Tetragonal Bipyramid ◽  
Scanning Electron

AbstractIn this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.

scholarly journals Capture and scanning electron microscopy of individual snow crystals

1994 ◽  
Vol 40 (134) ◽  
pp. 195-197
Author(s):  
E. W. Wolff ◽  
A. P. Reid
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Liquid Nitrogen Temperature ◽  
Experimental Methods ◽  
Snow Crystal ◽  
Snow Crystals ◽  
First Time ◽  
Scanning Electron

AbstractA snow crystal has been successfully collected on to a scanning electron microscope (SEM) stub in central Greenland. It was preserved at liquid-nitrogen temperature for 5 months, prior to examination in the SEM. This is believed to be the first time a snow crystal has been observed directly in the SEM and offers some new experimental methods for understanding crystals and their chemistry.

Ascospore sculpturing and generic concepts in the Testudinaceae (syn. Zopfiaceae)

1979 ◽  
Vol 57 (2) ◽  
pp. 91-99 ◽  
Author(s):  
D. L. Hawksworth
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Combination ◽  
The Family ◽  
First Time ◽  
Scanning Electron

Generic concepts in the Testudinaceae (Ascomycotina, Loculoascomycetes) are reviewed with particular emphasis on the sculpturing of the ascospores which has been examined by scanning electron microscopy (SEM) for the first time. A key to the nine genera accepted in the family is provided of which two are described as new: Ulospora for U. bilgramii comb.nov. (= Zopfia bilgramii D. Hawksw. et al.), the ascospores of which have three to six deep fissures in each cell, and Zopfiofoveola for Z. punctata comb.nov. (= Zopfia punctata D. Hawksw. & C. Booth), with distinctly and regularly foveolate ascospores. One further new combination is made: Rechingeriella boudieri comb.nov. (= Zopfia boudieri Arnaud). The positions of three additional genera referred to the family by earlier authors but which are excluded from it here are also briefly discussed.

Statoblast morphology and systematics of the freshwater bryozoan Hyalinella orbisperma (Kellicott, 1882)

1992 ◽  
Vol 70 (8) ◽  
pp. 1536-1540 ◽  
Author(s):  
Anthony Ricciardi ◽  
Timothy S. Wood
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Relationship ◽  
Georgian Bay ◽  
Close Phylogenetic Relationship ◽  
First Time ◽  
Scanning Electron

The freshwater bryozoan Hyalinella orbisperma (Ectoprocta: Phylactolaemata), previously known only from Michigan, is added to the list of Canadian fauna from a specimen collected at Georgian Bay, Ontario. Floatoblasts match the appearance of those described from Michigan. The sessoblast of this species is recorded and illustrated for the first time. The case for other species with sessoblasts in the genus Hyalinella is extremely weak. Scanning electron microscopy of the floatoblast and sessoblast reveals a raised reticulation with interstitial tubercles covering the capsule periblast, suggesting a close phylogenetic relationship with certain Plumatella species. It is proposed that H. orbisperma be reassigned to the genus Plumatella.

New perspectives on Nephridiacanthus major (Acanthocephala: Oligacanthorhynchidae) collected from hedgehogs in Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
O.M. Amin ◽  
M. Sharifdini ◽  
R.A. Heckmann ◽  
M. Zarean
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Chemical Analysis ◽  
Central Asia ◽  
Molecular Profile ◽  
X Ray ◽  
The Family ◽  
First Time ◽  
Scanning Electron

We describe morphological features not previously reported for this old acanthocephalan Nephridiacanthus major (Bremser, 1811 in Westrumb, 1821) Golvan, 1962 first described over 200 years ago. Our specimens were collected from long-eared hedgehog Hemiechinus auritus (Gmelin, 1770) (Erinaceidae) in Iran. We compare the morphometrics of our material with others previously reported from the same host in Iran, Russia, central Asia and Europe. Our specimens had markedly smaller proboscides, proboscis hooks and lemnisci than those reported from Russia and central Asia, but comparable measurements of other structures with specimens previously described from other collections. We document our new observations with scanning electron microscopy features not previously demonstrable by other observers and provide a chemical analysis of proboscis hooks using energy-dispersive X-ray analysis for the first time. The molecular profile of this acanthocephalan, based on 18S rDNA and cox1 genes, was generated for the first time. The phylogenetic analysis showed that N. major is placed in a clade of the family Oligacanthorhynchidae, well separated from the families Moniliformidae and Gigantorhynchidae.

scholarly journals Petal and Sepal Epidermal Micromorphology of Six Lathyrus Taxa (Fabaceae) and their Systematic Value

2012 ◽  
Vol 40 (1) ◽  
pp. 35 ◽  
Author(s):  
Hüseyin CILDIR ◽  
Ahmet KAHRAMAN ◽  
Musa DOGAN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Glandular Trichomes ◽  
Systematic Significance ◽  
Trichome Types ◽  
First Time ◽  
Scanning Electron

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.

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