Invasion of chicken caecal and intestinal lamina propria by crypt epithelial cells infected with coccidia

Parasitology ◽  
1983 ◽  
Vol 86 (3) ◽  
pp. 391-398 ◽  
Author(s):  
M. A. Fernando ◽  
A. M. Lawn ◽  
M. Elaine Rose ◽  
M. A. Al-Attar
Keyword(s):  
Electron Microscope ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Lamina Propria ◽  
First Generation ◽  
Second Generation ◽  
Intestinal Lamina Propria ◽  
Epithelial Origin ◽  
Infected Cells ◽  
Crypt Cells

SUMMARYThe development of second generation schizonts of Eimeria necatrix and E. tenella was studied with the electron microscope. Invasion of the crypt epithelial cells by merozoites of the first generation schizonts caused changes in the morphology of the infected cells and stimulated their migration into the lamina propria through breaks which appeared in the basement membrane of the crypts. Second generation schizonts developed in the lamina propria within these crypt cells whose epithelial origin was confirmed by their interconnection by desmosomes and tight junctions and by their possession of characteristic microvilli. A comparison is made between this invasion of the lamina propria by parasitized cells and invasion of connective tissue by malignant epithelial cells; the possible mechanisms involved are discussed.

Fine structural aspects of an embryo-adapted strain of Eimeria tenella in the epithelial cells of the chorio-allantoic membrane

Parasitology ◽  
1978 ◽  
Vol 76 (2) ◽  
pp. 185-191 ◽  
Author(s):  
I. G. Lücht ◽  
B. J. Millard ◽  
E. G. Scholtyseck
Keyword(s):  
Epithelial Cells ◽  
First Generation ◽  
Second Generation ◽  
Developmental Stages ◽  
Eimeria Tenella ◽  
Ultrastructural Changes ◽  
Strain Development ◽  
Circular Structure ◽  
Structural Aspects ◽  
Endogenous Stages

SummaryThe fine structure and development of the endogenous stages of an embryo-adapted strain of Eimeria tenella in chicken embryos is described. First-generation merozoites were formed by endodyogeny in uninucleate developmental stages as well as schizogony and the endoplasmic reticulum of merozoites of the second-generation schizonts appeared as a circular structure, often enclosing other organelles. In contrast to the parent strain, development of the second-generation schizonts was restricted to cells of epithelial origin. These observations confirm that the complete endogenous cycle of the embryo-adapted strain of E. tenella is restricted to epithelial cells of the chorio-allantoic membrane (CAM) and that no major ultrastructural changes have occurred as a result of repeated embryo passage.

Hymenolepis citelli, H. diminuta and H. microstoma: immunoglobulin-containing cells in the lamina propria of the mouse gut during primary and secondary infections

1987 ◽  
Vol 61 (4) ◽  
pp. 329-340 ◽  
Author(s):  
Sidi T. O. Alghali
Keyword(s):  
Epithelial Cells ◽  
Immune Responses ◽  
Lamina Propria ◽  
Uninfected Control ◽  
Intestinal Lamina Propria ◽  
Secondary Infections ◽  
Functional Immunity ◽  
Immunofluorescent Technique ◽  
The Mean ◽  
Indirect Immunofluorescent

ABSTRACTThe indirect immunofluorescent technique was used to determine the occurrence of IgA, IgM and IgG1 immunoglobulin-containing cells in local intestinal mucosal immune responses to Hymenolepis citelli, H. diminuta and H. microstoma infections in mice. In the intestinal lamina propria of H. citelli and H. diminuta infected mice there was no increase in the mean numbers of immunoglobulin-containing cells when compared with uninfected control mice, but there was in H. microstoma infected mice. The numbers of IgG1 – positive cells in both infected and uninfected mice were very small relative to IgA and IgM immunocytes. The distribution of immunocytes in the lamina propria of infected and uninfected mice was essentially similar and the localization of isotypes in duodenal sections showed no immunoglobulins in the villous epithelial cells. There was also no marked difference between primary and secondary infections indicating that immunoglobulin-containing cells play no major role in functional immunity against hymenolepid infections in the mouse. The presence of IgA and IgM was also demonstrated on the tegument of the tapeworms, although the distribution was patchy and more abundant on H. microstoma than on H. diminuta or H. citelli. The time of appearance of both isotypes was latest on H. citelli.

scholarly journals Morphological and histochemical features of the cloaca of Turkey hen Meleagris Gallopavo

2017 ◽  
Vol 41 (1) ◽  
pp. 28-33
Author(s):  
Lamees Ezaldeen Mohammed
Keyword(s):  
Epithelial Cells ◽  
Connective Tissue ◽  
Lamina Propria ◽  
Positive Reaction ◽  
Alcian Blue ◽  
Smooth Muscles ◽  
Meleagris Gallopavo ◽  
Loose Connective Tissue ◽  
The Mean ◽  
Columnar Cells

     The study includes six adult healthy hen turkey. The cloaca has three compartments separated by two circular folds and next to rectum is the coprodeum which the largest and measured (2.5±0.2cm) in length and (2.1±0.1cm) in diameter. The middle and smallest is the urodeum that measured about (1.01±0.2cm) in length and (1.2±1cm.) in diameter, that receives the ureter and left oviduct. Posteriorly, the proctodeum that measured about (1.4±0.1cm) in length and (1.6±1cm.) in diameter. The coprodeum mucosa has short and flat villi. The urodeum mucosa has tall branching pyramidal mucosal villi. The mucosa of proctodeum has lost the villi pattern. The three compartments were lined with mucous secreting columnar cells, most of these epithelial cells were positive for Alcian blue (2.5pH) stain and few were positive for PAS stain. The epithelial tubular glands showed positive reaction for Alcian blue satin only. The lamina propria of these compartments was cellular loose connective tissue displayed numerous of lymphatic nodules. The muscular mucosa is lost in proctodeum only. Tunica muscular is composed of two layers of smooth muscles fibers in coprodeum and urodeum and turned into skeletal type in proctodeum. The mean height of mucosal villi has significantly increased in urodeum.

scholarly journals Distribution of urokinase-type plasminogen activator immunoreactivity in the mouse.

1984 ◽  
Vol 98 (3) ◽  
pp. 894-903 ◽  
Author(s):  
L I Larsson ◽  
L Skriver ◽  
L S Nielsen ◽  
J Grøndahl-Hansen ◽  
P Kristensen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Connective Tissue ◽  
Plasminogen Activator ◽  
Lamina Propria ◽  
Protein Band ◽  
Good Correspondence ◽  
Ductus Deferens ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Decidual Cells

Immunocytochemistry, using rabbit antibodies to a urokinase-type 48-Kdalton Mr mouse plasminogen activator, showed that enzyme immunoreactivity is widely distributed in the normal mouse. Strong staining was obtained in widely disseminated connective tissue cells with a fibroblast-like morphology. Such cells occurred in high numbers in the lamina propria mucosae of the gastrointestinal tract, and in moderate numbers in the connective tissue septa of the pancreas. A few such cells were detected around the larynx, trachea, and bronchi. Immunoreactivity also occurred in epithelial cells of the proximal and distal kidney tubules, the ductus deferens, and in pulmonary pneumocytes. In addition, presumably extracellular staining was seen irregularly along the basement membrane and fibrillar structures in the lamina propria of the small and large intestines. Moreover, decidual cells of the mouse placenta stained strongly, and a moderate staining was observed in epithelial cells of involuting mammary glands, but not in those of noninvoluting glands. No immunoreactivity was observed in endothelial cells. Control experiments included absorption of the antibodies against highly-purified mouse plasminogen activator and the corresponding proenzyme, and the finding of a good correspondence between the number of immunoreactive cells and measurable enzymatic activity determined in adjacent tissue sections. Separation by SDS PAGE followed by immunoblotting revealed only one immunochemically stainable protein band with Mr approximately 48 Kdaltons in extracts from tissues showing immunoreactivity.

Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

1967 ◽  
Vol 25 ◽  
pp. 110-111
Author(s):  
W. G. Banfield ◽  
G. Kasnic ◽  
J. H. Blackwell
Keyword(s):  
Electron Microscope ◽  
Infected Cell ◽  
Microscopic Study ◽  
Intestinal Epithelium ◽  
Ultrastructural Study ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

Adenomatoid Tumor of the Testis, A Mesonephric Hamartoma

1971 ◽  
Vol 29 ◽  
pp. 318-319
Author(s):  
Raoul Fresco ◽  
Mary Chang-Lo
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Fibrous Tissue ◽  
Salient Feature ◽  
Luminal Surface ◽  
Adenomatoid Tumor ◽  
Ultrastructural Studies ◽  
Epithelial Origin ◽  
Brush Borders ◽  
Cell Processes

Confusion surrounds the nature of the “adenomatoid tumor” of the testis, as evidenced by the large number of synonyms which have been ascribed to it. Various authors have considered the tumor to be of endothelial, mesothelial or epithelial origin. There appears to be no controversy as to the stromal elements of the tumor, which consists mainly of smooth muscle and fibrous tissue. It is the irregular gland-like spaces which have given rise to the numerous theories as to its histogenesis, and even recent ultrastructural studies fail to agree on the origin of these structures.Electron microscopy of a typical intrascrotal adenomatoid tumor showed the gland-like spaces to be lined by epithelial cells (Fig. 1), rich in cytoplasmic tonofibrils and united to each other by numerous desmosomes (Fig. 2). The most salient feature of these epithelial cells was the presence on their luminal surface of numerous long and repeatedly branching microvillous structures of the type known as stereocilia (Fig. 3). These are extremely long slender cell processes which are as much as three to four times the length of those in brush borders.

The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

1995 ◽  
Vol 53 ◽  
pp. 818-819
Author(s):  
D.S. Friend ◽  
N. Ghildyal ◽  
M.F. Gurish ◽  
K.F. Austen ◽  
R.L. Stevens
Keyword(s):  
Small Intestine ◽  
Mast Cells ◽  
Epithelial Cells ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial ◽  
Carboxypeptidase A ◽  
C3h Mice ◽  
Granule Morphology ◽  
Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

Ultrastructure Research of the Gastritis from Returning Bile

1990 ◽  
Vol 48 (3) ◽  
pp. 202-203
Author(s):  
Shaopeng Hu ◽  
Jianhua Wang ◽  
Zhen Li ◽  
Huei Chen ◽  
Fei Cu ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Gastric Mucosa ◽  
Scanning Electron Microscope ◽  
Lamina Propria ◽  
Transmission Electron Microscope ◽  
Ultrastructural Change ◽  
Common Disease ◽  
Epithelial Surface ◽  
Transmission Electron ◽  
Scanning Electron

Gastritis from returning bile is a common disease, but the reason for the disease is not clear. As the pathologic ultrastructure research progresses, it has drawn attention to the ultrastructural change of cells in gastric mucosa by clinical workers. We observed gastric mucosa tissues of 15 patients suffering from gastritis with a transmission electron microscope (TEM) and a scanning electron microscope (SEM). It is the first report in China that fungus exists in the lamina propria of gastric mucosa tissue. The result is as follows.The gastric mucosa tissues of 15 patients suffering from gastritis were acquired by stomachoscopy. Both TEM and SEM specimens were prepared by the usual methods. Under the TEM, the epithelial surface became higher and larger. Mitochondria of the cells were swollen and cristae were disrupted. There were vacuoles in the cells. The nucleus showed disorder, heterochromatin became darker, and nucleolae could be observed.

Sign in / Sign up

Export Citation Format

Share Document