Detection ofNeospora caninumin aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China

Parasitology ◽  
2009 ◽  
Vol 136 (11) ◽  
pp. 1251-1256 ◽  
Author(s):  
L. YAO ◽  
N. YANG ◽  
Q. LIU ◽  
M. WANG ◽  
W. ZHANG ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Diagnostic Tool ◽  
Nested Pcr ◽  
Neospora Caninum ◽  
Primary Diagnosis ◽  
Dairy Herds ◽  
Pcr Assay ◽  
Blood Samples ◽  
Bovine Fetuses ◽  
The Brain

SUMMARYNeospora caninuminfection is a significant cause of abortion in cattle. We investigated the tissue distribution ofN. caninumin aborted bovine fetuses and dam blood samples by a nested PCR assay, and compared the nested PCR with ELISA in the diagnosis ofN. caninuminfection. In total, 26 aborted fetuses and 813 blood samples were collected from 8 dairy herds in Beijing (n=212) and Tianjin (n=601), China. Fifteen fetuses (57·7%) were testedN. caninum-positive by the nested PCR.N. caninumDNA was detected from the brain of 52%, kidneys of 22%, skeletal muscle of 18%, and heart of 4% of the aborted fetuses. The PCR-positive cases (55%, 11/20) were higher than seropositive cows (40%, 8/20) in a subset of 20 fetuses, but the PCR results of blood samples of the 20 cows were all negative. The seroprevalence of the 813 samples was 15·5% (43·4% of samples from Beijing, 5·7% of samples from Tianjin), compared to the PCR-positive blood samples of 0·9%. Our study showed that the nested PCR is a valuable diagnostic tool for the primary diagnosis ofN. caninumin aborted fetuses, while ELISA is the preferred assay for testing blood samples collected from cows. The two assays are complementary in determining whether abortions are associated withN. caninuminfection in cattle.

scholarly journals Neospora caninum in bovine fetuses of Minas Gerais, Brazil: genetic characteristics of rDNA

2011 ◽  
Vol 20 (4) ◽  
pp. 281-288 ◽  
Author(s):  
Domingos Sávio dos Santos ◽  
Michele Placedino Andrade ◽  
Mary Suzan Varaschin ◽  
Antônio Marcos Guimarães ◽  
Christian Hirsch
Keyword(s):  
Skeletal Muscle ◽  
Genetic Differentiation ◽  
Phylogenetic Tree ◽  
Neospora Caninum ◽  
Minas Gerais ◽  
Genetic Characteristics ◽  
Three Samples ◽  
Bovine Fetuses ◽  
Host Parasite ◽  
The Brain

Neosporosis is an important cause of abortion in cattle and information on their genetics and host parasite relationships are desirable. Neospora caninum samples obtained from 24 bovine fetuses from Minas Gerais, were genetically analyzed in part of the rDNA region, coding for rRNAs. Previously, brain, heart, liver, skeletal muscle, lung, kidney, spleen, thymus, lymph nodes, ovary or testis, uterus and skin of the ear were analyzed by conventional histopathology and immunohistochemistry. Of these, eight had lesions compatible in the brain and heart and two also in skeletal muscle and liver. Three brains showed antigen identification in immunohistochemistry. Brain and heart tissues were subjected to DNA extraction for PCR, whose product of 588 bp of ITS-1 region was sequenced in three samples. We obtained 96% similarity with dozens of sequences N. caninum deposited in GenBank. The phylogenetic tree showed great conservation among isolates of N. caninum in this study and those deposited in GenBank, while well-defined and specific branches were generated against other Apicomplexa. PCR for this region is useful as a diagnosis, with good analytical specificity, but the ITS-1 region is not suitable for genetic differentiation intra species because the sequences obtained were identical to the others analyzed.

A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals

2009 ◽  
Vol 106 (2) ◽  
pp. 357-365 ◽  
Author(s):  
David O. Odongo ◽  
Jack D. Sunter ◽  
Henry K. Kiara ◽  
Robert A. Skilton ◽  
Richard P. Bishop
Keyword(s):  
Nested Pcr ◽  
Theileria Parva ◽  
Bovine Blood ◽  
Pcr Assay ◽  
Blood Samples ◽  
Enhanced Sensitivity

scholarly journals Outbreak of caprine abortion by Toxoplasma gondii in Midwest Brazil

2011 ◽  
Vol 31 (11) ◽  
pp. 933-937 ◽  
Author(s):  
Flávio Henrique Bravim Caldeira ◽  
Daniel Guimarães Ubiali ◽  
Isabela de Godoy ◽  
Valéria Dutra ◽  
Daniel Moura de Aguiar ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Brucella Abortus ◽  
Nested Pcr ◽  
Neospora Caninum ◽  
Pcr Assay ◽  
Serum Samples ◽  
Pathology Laboratory ◽  
Mato Grosso ◽  
Veterinary Pathology ◽  
Gross Lesions

An outbreak of abortion by Toxoplasma gondii in goats on a farm in the Brazilian Midwest is reported. Gross lesions were not observed in seven aborted fetuses submitted to the Veterinary Pathology Laboratory, Federal University of Mato Grosso, for necropsy investigation. The main histologic lesions were mononuclear cell pneumonia and necrotizing encephalitis in varying degrees of intensity. PCR for Brucella abortus and Neospora caninum and aerobic cultures were negative in all cases. Antibody titles against T. gondii varying from 1:1024 to 1:32.768 were detected in serum samples from four aborted goats. Nested-PCR assay for T. gondii were positive in brain samples of all cases submitted. These findings indicate that T. gondii infection should be considered in the diagnosis of abortion in goats in Midwest Brazil.

scholarly journals Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR

2009 ◽  
Vol 18 (04) ◽  
pp. 14-19 ◽  
Author(s):  
A. D. Cabral ◽  
C. N. Camargo ◽  
N. T. C. Galleti ◽  
L. H. Okuda ◽  
E. M. Pituco ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Neospora Caninum ◽  
Bovine Fetuses

scholarly journals Current trends in bovine abortion in Argentina

2019 ◽  
Vol 39 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Eleonora L. Morrell ◽  
Carlos M. Campero ◽  
Germán J. Cantón ◽  
Anselmo C. Odeón ◽  
Dadín P. Moore ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Economic Losses ◽  
Dairy Herds ◽  
Diarrhea Virus ◽  
Current Trends ◽  
Infectious Origin ◽  
Bovine Fetuses ◽  
Bovine Abortion

ABSTRACT: Bovine abortion is an important cause of significant economic losses in beef and dairy herds. This syndrome is usually difficult to diagnose. The aim of this study was to characterize bovine abortion causes in Argentina by standard diagnosis procedures (histology, bacterial and viral isolation) and other diagnostic tests like direct fluorescent antibody test (DFAT), fetal serology, immunohistochemistry (IHC), and PCR, showing their specific advantages and limitations. Necropsies were performed in 150 aborted bovine fetuses submitted to the diagnostic laboratories of Instituto Nacional de Tecnología Agropecuaria (INTA) Balcarce, Argentina. Etiological diagnosis was confirmed in 78 fetuses (52% of the cases). Most causes of abortion were of infectious origin, being Neospora caninum (14.67%), Campylobacter fetus sp. (9.33%), Leptospira spp. (7.33%) and Brucella abortus (6.65%) the main microorganisms identified. Bovine viral diarrhea virus (BVDV) and bovine herpes virus (BHV) were diagnosed in 2 (1.33%) and 3 (2%) cases, respectively. This study showed a better characterization of bovine abortion compared with previous researches done on this topic.

scholarly journals The occurrence of anti-Neospora caninum in bovine female animals and bovine fetuses in Nossa Senhora do Livramento County, Mato Grosso

2016 ◽  
Vol 37 (6) ◽  
pp. 4161
Author(s):  
Robson Souza Rodrigues ◽  
Michelle Igarashi ◽  
Livia Saab Muraro ◽  
Ana Helena Benetti Gomes ◽  
Daniel Moura Aguiar ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Bos Indicus ◽  
Parasitic Disease ◽  
Immunofluorescence Technique ◽  
Mato Grosso ◽  
Blood Samples ◽  
Obligate Intracellular ◽  
Bovine Fetuses

Neosporosis is a parasitic disease caused by the obligate intracellular protozoan Neospora caninum, which is responsible for miscarriage cases in cattle countrywide. The vertical or transplacental route with prevalent miscarriages between the 3rd and 6rd months of pregnancyis the main transmission form in cattle. The aim of the present study is to investigate the presence of anti-N.caninum antibodies in (Bos indicus) Nellore female animals and fetuses in Nossa Senhora do Livramento County - MT. Blood samples from 489 female bovines and from 128 bovine fetuses over three months of pregnancy were colleted. The research about the anti N. caninum antibodies in bovine females and fetuses was conducted through the Immunofluorescence technique (IFA). We found 2.45% (12) seropositive and 3.91% (5) seropositive to N. caninum in the present study.

Survey of nine abortifacient infectious agents in aborted bovine fetuses from dairy farms in Beijing, China, by PCR

2012 ◽  
Vol 60 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Na Yang ◽  
Xia Cui ◽  
Weifeng Qian ◽  
Shanshan Yu ◽  
Qun Liu
Keyword(s):  
Neospora Caninum ◽  
Bovine Viral Diarrhoea Virus ◽  
Economic Losses ◽  
Dairy Herds ◽  
Infectious Agents ◽  
Infectious Bovine Rhinotracheitis ◽  
Diarrhoea Virus ◽  
Bovine Fetuses ◽  
Bovine Abortion ◽  
Infectious Bovine Rhinotracheitis Virus

Abortion in dairy cattle causes considerable economic losses to the dairy industry. Aborted fetuses and samples from the corresponding aborting dams from 12 dairy herds in Beijing were tested for 9 abortifacient infectious pathogens by PCR between 2008 and 2010. From a total of 80 abortion cases collected during this period, infectious agents were detected in 45 (56.3%) cases, 22 (48.9%) of which represented co-infections with two or three infectious agents. The detected pathogens included infectious bovine rhinotracheitis virus (36.3%) andNeospora caninum(31.3%), followed by bovine viral diarrhoea virus (7.5%),Brucella abortus(6.3%),Tritrichomonas foetus(5%) andToxoplasma gondii(1.3%).Campylobacter fetus, Coxiella burnetiiandChlamydophila psittaciwere not detected in any abortion case. Findings from this study indicated that infectious bovine rhinotracheitis virus andNeospora caninumwere the main potential causes of abortions in Beijing dairy herds, whereas the bacterial pathogens were not, in contrast to reports from other countries. This is the first study to test nine abortifacient infectious agents by PCR at the same time, and it is also the first time to report the involvement of a variety of infectious agents in bovine abortion cases in China.

scholarly journals An abortion storm in dairy cattle associated with neosporosis in southern Brazil

2021 ◽  
Vol 30 (2) ◽  
Author(s):  
João Henrique Perotta ◽  
Bárbara Barbi de Freitas ◽  
Nicoly Nayana Marcom ◽  
Caroline Argenta Pescador ◽  
Cláudia Carnielli Pereira ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Dairy Herd ◽  
Eastern Region ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Southern Brazil ◽  
Antibody Technique ◽  
Blood Samples ◽  
The Brain

Abstract Between December 2016 and April 2017, a spate of abortions occurred in a closed dairy herd from the central eastern region of Paraná, Brazil, in which 75 cows aborted. To identify its cause, organ fragments were collected from an aborted fetus for histopathology, and the blood samples from a stillborn, 4 aborted fetuses, and 9 farm dogs for indirect fluorescent antibody technique (IFAT). These tests found multifocal non-suppurative encephalitis, periportal hepatitis, and multifocal lymphoplasmacytic myocarditis, and detected anti-Neospora antibodies in all aborted fetuses, and in 5 of the 9 dogs. DNA of Neospora caninum was detected in the brain tissue of an aborted fetus. Blood samples of 340 cows and 146 heifers showed 33.5% and 30.8% seropositivity, respectively. In this closed herd, the parasite was probably introduced by infected domesticated or wild carnivores inhabiting the farm, through the infective oocysts present in their stool.

scholarly journals Bovine Fetal Neosporosis: A Comparison of Epizootic and Sporadic Abortion Cases and Different Age Classes with Regard to Lesion Severity and Immunohistochemical Identification of Organisms in Brain, Heart, and Liver

1997 ◽  
Vol 9 (2) ◽  
pp. 180-185 ◽  
Author(s):  
W. Wouda ◽  
A. R. Moen ◽  
I. J. R. Visser ◽  
F. van Knapen
Keyword(s):  
Neospora Caninum ◽  
Age Classes ◽  
Hepatocellular Necrosis ◽  
Age Related ◽  
Bovine Fetuses ◽  
Hepatic Lesions ◽  
The Brain ◽  
Caninum Tachyzoites ◽  
Lesion Severity ◽  
Immunohistochemical Identification

Eighty bovine fetuses with confirmed neosporosis were used to score lesion severity and presence of parasites in brain, heart, and liver. A comparison was made between epizootic and sporadic abortion cases. The possible influence of fetal age was also investigated. Histologic lesions of multifocal encephalitis, myocarditis, and periportal hepatitis with or without focal hepatocellular necrosis were almost always observed. Neospora caninum tachyzoites were identified immunohistochemically in 85% of the brains, 14% of the hearts, and 26% of the livers. Tissue cysts were observed in 21% of the brains. Significant differences between epizootic and sporadic abortion cases were found only in the liver. Hepatic lesions were more prominent and N. caninum tachyzoites were observed more frequently and in higher numbers in epizootic cases. Examination by immunohistochemistry of the liver in addition to the brain can be highly contributive diagnostically, particularly in epizootic cases. There were no significant age-related differences except for a higher presence of tachyzoites in the hearts of younger fetuses (3-4 months gestational age).

scholarly journals Molecular Identification of Neospora caninum Infection in Abort-ed Fetuses of Sheep, Cattle, and Goats in Mazandaran Province, Northern Iran

2021 ◽  
Author(s):  
Behnaz Salehi ◽  
Afsaneh Amouei ◽  
Samira Dodangeh ◽  
Ahmad Daryani ◽  
Shahabeddin Sarvi ◽  
...  
Keyword(s):  
Prevalence Rate ◽  
Neospora Caninum ◽  
Isoamyl Alcohol ◽  
Economic Loss ◽  
Pcr Assay ◽  
Mazandaran Province ◽  
Livestock Farming ◽  
Northern Iran ◽  
Caninum Infection ◽  
The Brain

Background: We aimed to identify Neospora caninum DNA in the brain samples of aborted fetuses of cattle, goats, and sheep in Mazandaran, northern Iran, using PCR. Methods: In total, 133 aborted fetuses (51 sheep, 78 cattle, and 4 goats) were randomly collected from different stages of gestation in various regions of Mazandaran, Iran, from Mar 2016 to May 2017. The DNA was extracted from all the brain samples using phenol chloroform isoamyl alcohol instructions. The Nc-5 gene was used for the detection of N. caninum DNA by nested-PCR assay. Results: The detection of N. caninum DNA was confirmed by the observation of a 227 bp band in 24 samples of 133 aborted fetuses (18.1%). The highest prevalence rate of N. caninum was detected in the cattle (20.5%) followed by the sheep (15.6%); however, no positive cases were reported in the goats. The highest and lowest prevalence rates of the infection were reported as 23.8% and 8.6% in Qaemshahr, and Behshahr, respectively. The prevalence rate of infection (32%) in the early gestational period was higher than those in the middle (15%) and late (3.8%) gestational periods. Conclusion: The obtained data of the present study indicated that N. caninum infection may partly be responsible for abortion and economic loss in livestock farming in Mazandaran Province.

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